ES2191709T3 - Procedimiento de produccion de la proteasa del virus herpes simplex 1 o 2. - Google Patents

Procedimiento de produccion de la proteasa del virus herpes simplex 1 o 2.

Info

Publication number
ES2191709T3
ES2191709T3 ES95927255T ES95927255T ES2191709T3 ES 2191709 T3 ES2191709 T3 ES 2191709T3 ES 95927255 T ES95927255 T ES 95927255T ES 95927255 T ES95927255 T ES 95927255T ES 2191709 T3 ES2191709 T3 ES 2191709T3
Authority
ES
Spain
Prior art keywords
herpes simplex
simplex virus
procedure
production
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95927255T
Other languages
English (en)
Inventor
Paul L Darke
Dawn L Hall
Lawrence C Kuo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Application granted granted Critical
Publication of ES2191709T3 publication Critical patent/ES2191709T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

SE INVESTIGA UN METODO DE PURIFICACION DE PROTEASAS DEL HERPES SIMPLEX VIRUS 1 Y 2. SE DUPLICA UNA PROTEASA PRECURSORA EN UNA CELULA HUESPED Y A CONTINUACION SE SEPARA DEL CULTIVO DE CELULA MEDIANTE CROMATOGRAFIA DE INTERCAMBIO DE CATION. LA PROTEASA RESULTANTE SE SOMETE A CONDICIONES DE AUTOPROCESAMIENTO Y OCURRE UNA SEPARACION AUTOLITICA, DANDO COMO RESULTADO UNA PROTEASA MADURA.
ES95927255T 1994-07-21 1995-07-17 Procedimiento de produccion de la proteasa del virus herpes simplex 1 o 2. Expired - Lifetime ES2191709T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/279,754 US5486470A (en) 1994-07-21 1994-07-21 Purified herpes simplex virus protease and methods of purification

Publications (1)

Publication Number Publication Date
ES2191709T3 true ES2191709T3 (es) 2003-09-16

Family

ID=23070306

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95927255T Expired - Lifetime ES2191709T3 (es) 1994-07-21 1995-07-17 Procedimiento de produccion de la proteasa del virus herpes simplex 1 o 2.

Country Status (8)

Country Link
US (1) US5486470A (es)
EP (1) EP0777727B1 (es)
JP (1) JPH10503648A (es)
AT (1) ATE233315T1 (es)
CA (1) CA2193851A1 (es)
DE (1) DE69529754T2 (es)
ES (1) ES2191709T3 (es)
WO (1) WO1996003498A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6083711A (en) * 1996-05-15 2000-07-04 Smithkline Beecham Corporation Proteases compositions capable of binding to said site, and methods of use thereof
US5965358A (en) * 1998-08-26 1999-10-12 Genvec, Inc. Method for assessing the relative purity of viral gene transfer vector stocks
AU3731400A (en) 1999-03-05 2000-09-21 Trustees Of University Technology Corporation, The Methods and compositions useful in inhibiting apoptosis
AU3864000A (en) * 1999-03-05 2000-09-21 Trustees Of University Technology Corporation, The Inhibitors of serine protease activity, methods and compositions for treatment of herpes viruses
US6489308B1 (en) 1999-03-05 2002-12-03 Trustees Of University Of Technology Corporation Inhibitors of serine protease activity, methods and compositions for treatment of nitric-oxide-induced clinical conditions
US6849605B1 (en) 1999-03-05 2005-02-01 The Trustees Of University Technology Corporation Inhibitors of serine protease activity, methods and compositions for treatment of viral infections
ITBO20010426A1 (it) * 2001-07-06 2003-01-06 Alfa Wassermann Spa Processo per la purificazione di proteine farmacologicamente attive mediante cromatografia in scambio cationico
US20040220242A1 (en) * 2003-05-02 2004-11-04 Leland Shapiro Inhibitors of serine protease activity, methods and compositions for treatment of nitric oxide induced clinical conditions
US20130156884A1 (en) * 2011-12-19 2013-06-20 Triarco Industries Protease enzymes for increased protein digestion rate and absorption and methods of using the same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ242739A (en) * 1991-05-24 1994-12-22 Arch Dev Corp Identification and purification of herpes protease nucleic acid segments and their use in the production of this protease
US5434074A (en) * 1991-07-05 1995-07-18 Gibson; D. Wade Cytomegalovirus proteinase

Also Published As

Publication number Publication date
US5486470A (en) 1996-01-23
ATE233315T1 (de) 2003-03-15
DE69529754D1 (de) 2003-04-03
WO1996003498A1 (en) 1996-02-08
EP0777727B1 (en) 2003-02-26
CA2193851A1 (en) 1996-02-08
EP0777727A1 (en) 1997-06-11
JPH10503648A (ja) 1998-04-07
EP0777727A4 (en) 1999-01-27
DE69529754T2 (de) 2003-10-16

Similar Documents

Publication Publication Date Title
DE3587168D1 (de) Glucoamylasegen.
AU638133B2 (en) Ribosome inactivating proteins, inactive precursor forms thereof, a process for making and a method of using
FI922434A0 (fi) Produktion av rekombinant human interleukin-1-inhibitor.
ES2001542A6 (es) Procedimiento de sulfatacion de glicosaminoglicanos
GR3019492T3 (en) Process for self-cleaning of a reactor chamber
ES2128067T3 (es) Detergente en bloque que contiene acido nitrilotriacetico.
IL85954A0 (en) Novel proteolytic enzymes
DE3574396D1 (en) Solid soap and a process for the production thereof
ES8400143A1 (es) Procedimiento para preparar 1,2-dihidroxi-ciclohexadienos.
ATE233315T1 (de) Produktionsmethoden für herpes simplex virus 1 oder 2 protease
BG106768A (en) Process for the purification of pharmacologically active proteins by cation-exchange chromatography
DE3784728D1 (de) Reinigung von rekombinantem interleukin-1.
AU3037192A (en) Recombinant gene coding for human alpha interferon and expression vector thereof, etc.
BR9916627A (pt) Processo para produzir transglutaminase tendoatividade enzimática, e, protéina
EP0228274A3 (en) Method for producing 2-keto-d-glucaric-acid
WO1994026925A3 (en) Processes for producing an enzyme
ES2058287T3 (es) Ureasa acida y su produccion.
BR8506226A (pt) Processo para aperfeicoar a filtrabilidade de um caldo microbial
ES547765A0 (es) Procedimiento para la hidrolisis enzimatica de una amida de d-alfa-aminoacido.
TR27072A (tr) Sodyum hidroksid imali yöntemi.
IL90947A (en) Method for production of hiv protease in transformed cells, dna and vectors comprising said dna used in said production
ES8700652A1 (es) Un metodo de preparar n-4-(3-aminopropil)-aminobutil-2,2-di-hidroxietanoamida
IT1269977B (it) Purificazione del propofol
DE69031928D1 (de) Nachweis von heparinbindenden Seminalproteinen
GR3005611T3 (es)