ES2192080T3 - Caracterizacion de polipeptidos por escision y espectrometria de masa. - Google Patents

Caracterizacion de polipeptidos por escision y espectrometria de masa.

Info

Publication number
ES2192080T3
ES2192080T3 ES99947722T ES99947722T ES2192080T3 ES 2192080 T3 ES2192080 T3 ES 2192080T3 ES 99947722 T ES99947722 T ES 99947722T ES 99947722 T ES99947722 T ES 99947722T ES 2192080 T3 ES2192080 T3 ES 2192080T3
Authority
ES
Spain
Prior art keywords
polypeptides
mass spectrometry
polypeptide
excision
characterization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99947722T
Other languages
English (en)
Inventor
Gunter Schmidt
Andrew Hugin Thompson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xzillion GmbH and Co KG
Original Assignee
Xzillion GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xzillion GmbH and Co KG filed Critical Xzillion GmbH and Co KG
Application granted granted Critical
Publication of ES2192080T3 publication Critical patent/ES2192080T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método para caracterización de una población de polipéptidos cuyo método comprende: (a) poner en contacto una muestra que comprende polipéptidos con un primer agente de disociación para generar fragmentos de polipéptidos; (b) aislar uno o más fragmentos de polipéptidos, comprendiendo cada fragmento el término N o el término C del polipéptido a partir del cual se fragmentó; (c) identificar los fragmentos aislados por espectrometría de masas; (d) repetir las etapas (a)-(c) sobre la muestra utilizando un segundo agente de disociación que se disocia en un sitio diferente del primer agente de disociación; y (e) caracterizar uno o más polipéptidos en la muestra a partir de los fragmentos utilizados en las etapas (c) y (d).
ES99947722T 1998-10-01 1999-10-01 Caracterizacion de polipeptidos por escision y espectrometria de masa. Expired - Lifetime ES2192080T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9821393.7A GB9821393D0 (en) 1998-10-01 1998-10-01 Protein profiling 2

Publications (1)

Publication Number Publication Date
ES2192080T3 true ES2192080T3 (es) 2003-09-16

Family

ID=10839813

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99947722T Expired - Lifetime ES2192080T3 (es) 1998-10-01 1999-10-01 Caracterizacion de polipeptidos por escision y espectrometria de masa.

Country Status (16)

Country Link
US (1) US6846679B1 (es)
EP (1) EP1117999B1 (es)
JP (1) JP3730121B2 (es)
CN (1) CN1411555A (es)
AT (1) ATE232300T1 (es)
AU (1) AU768902B2 (es)
CA (1) CA2344642C (es)
DE (1) DE69905265T2 (es)
DK (1) DK1117999T3 (es)
ES (1) ES2192080T3 (es)
GB (1) GB9821393D0 (es)
IL (2) IL142314A0 (es)
NO (1) NO20011552L (es)
NZ (1) NZ510873A (es)
PT (1) PT1117999E (es)
WO (1) WO2000020870A1 (es)

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US6003511A (en) 1996-11-18 1999-12-21 Medlis Corp. Respiratory circuit terminal for a unilimb respiratory device
WO2001094935A2 (en) 2000-06-09 2001-12-13 Mds Proteomics, Inc. Labeling of proteomic samples during proteolysis for quantitation and sample multiplexing
EP1295123A4 (en) 2000-06-12 2005-02-02 Univ Washington SELECTIVE MARKING AND ISOLATION OF PHOSPHOPEPTIDES AND APPLICATIONS TO PROTEOMIC ANALYSIS
GB0014342D0 (en) * 2000-06-13 2000-08-02 Scient Analysis Instr Limited Method of analysis
JP2002168869A (ja) * 2000-12-05 2002-06-14 Sumitomo Chem Co Ltd ペプチドのアミノ酸配列の決定方法
JP4604345B2 (ja) * 2000-12-21 2011-01-05 住友化学株式会社 タンパク質のｎ末端のアミノ酸配列決定方法
US20020106700A1 (en) * 2001-02-05 2002-08-08 Foote Robert S. Method for analyzing proteins
DE10108892B4 (de) * 2001-02-23 2005-08-18 Graffinity Pharmaceuticals Ag Synthesevorrichtung und Verfahren zu deren Herstellung
EP1267170A1 (en) * 2001-06-07 2002-12-18 Xzillion GmbH & CO.KG Method for characterising polypeptides
DE60215498T2 (de) * 2001-06-07 2007-04-26 Electrophoretics Ltd., Cobham Verfahren zur bestimmung von polypeptiden
EP1265072A1 (en) * 2001-06-07 2002-12-11 Xzillion GmbH & CO.KG Method for characterising polypeptides
US20030064359A1 (en) * 2001-09-21 2003-04-03 Joel Lanoix Preparation of highly-purified plasma membranes
CA2460773C (en) 2001-09-24 2012-04-10 Atsuo F. Fukunaga Breathing circuits having unconventional respiratory conduits and systems and methods for optimizing utilisation of fresh gases
US7717109B2 (en) 2001-09-24 2010-05-18 F-Concepts Llc Breathing systems with post-inspiratory valve fresh gas flow input, components for implementing same, and methods of use
US7261105B2 (en) 2001-09-24 2007-08-28 F-Concepts Llc Breathing circuits having unconventional respiratory conduits and systems and methods for optimizing utilization of fresh gases
CN1623000A (zh) * 2001-11-29 2005-06-01 塞莫费尼根股份有限公司 多肽的定量分析方法
EP2317307B1 (en) 2002-02-14 2013-11-20 Ajinomoto Co., Inc. Method of analyzing amino- and/or imino-functional compounds, and analytical reagent
WO2003087839A1 (en) * 2002-04-04 2003-10-23 Xzillion Gmbh & Co. Kg Method for characterising analytes
CN1314968C (zh) * 2004-03-11 2007-05-09 复旦大学 痕量多肽或蛋白质富集及其直接分析方法
US7230235B2 (en) * 2005-05-05 2007-06-12 Palo Alto Research Center Incorporated Automatic detection of quality spectra
US20060249668A1 (en) * 2005-05-05 2006-11-09 Palo Alto Research Center Incorporated Automatic detection of quality spectra
GB0515323D0 (en) 2005-07-26 2005-08-31 Electrophoretics Ltd Mass labels
GB0518585D0 (en) 2005-09-12 2005-10-19 Electrophoretics Ltd Mass labels
CN101379074A (zh) * 2006-02-08 2009-03-04 日本电气株式会社 修饰肽的方法和鉴定肽的方法
EP1983346A4 (en) * 2006-02-08 2010-03-17 Nec Corp METHOD FOR CLEAVING A PEPTIDE BOND AT THE C-TERMINAL END OF A PEPTIDE, AND A METHOD FOR DETERMINING THE C-TERMINAL END-ACE AMINO ACID SEQUENCE OF A PEPTIDE
US20080319678A1 (en) * 2006-02-15 2008-12-25 University Of Virginia Patent Foundation Mass Tagging for Quantitative Analysis of Biomolecules using 13C Labeled Phenylisocyanate
EP2710380B1 (en) 2011-05-18 2017-06-28 Rudjer Boskovic Institute Method of detection of amino acid sequence and/or identification of peptides and proteins, by use of a new derivatization reagent and synthesis of 5-formyl-benzene-1,3-disulphonic acid as derivatization reagent
CN111208237B (zh) * 2018-11-21 2021-12-24 中国科学院大连化学物理研究所 基于肽段c末端化学衍生提高质谱碎裂效率和响应的方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2168478A (en) * 1984-11-26 1986-06-18 Scan Limited M Analysis of protein etc using mass spectrometry
US5792664A (en) * 1992-05-29 1998-08-11 The Rockefeller University Methods for producing and analyzing biopolymer ladders
JPH07507394A (ja) * 1992-05-29 1995-08-10 ザ ロックフェラー ユニバーシティ 質量スペクトロメータを用いてペプチドの配列決定を行う為の方法及び物質
US5470703A (en) * 1992-10-21 1995-11-28 Shimadzu Corporation Method for peptide C-terminal fragment sequence analysis and apparatus for collecting peptide fragment
JPH06172380A (ja) * 1992-12-04 1994-06-21 Shimadzu Corp ペプチドフラグメント分取装置
PT700521E (pt) * 1993-05-28 2003-10-31 Baylor College Medicine Metodo e espectrometro de massa para dessorcao e ionizacao de analitos
US5538897A (en) * 1994-03-14 1996-07-23 University Of Washington Use of mass spectrometry fragmentation patterns of peptides to identify amino acid sequences in databases
US5869240A (en) * 1995-05-19 1999-02-09 Perseptive Biosystems, Inc. Methods and apparatus for sequencing polymers with a statistical certainty using mass spectrometry
US5827659A (en) * 1995-05-19 1998-10-27 Perseptive Biosystems, Inc. Methods and apparatus for sequencing polymers using mass spectrometry
JP2001500606A (ja) 1995-05-19 2001-01-16 パーセプティブ バイオシステムズ，インコーポレーテッド 質量スペクトル分析を用いた統計的に確実性のあるポリマー配列決定のための方法および装置
US6245331B1 (en) * 1997-01-02 2001-06-12 New York Univ. Medical Center Early detection of mycobacterial disease
US6156527A (en) * 1997-01-23 2000-12-05 Brax Group Limited Characterizing polypeptides
US5824556A (en) * 1997-06-11 1998-10-20 Tarr; George E. Peptide mass ladders generated using carbon disulfide
US6379971B1 (en) * 1998-02-24 2002-04-30 Target Discovery, Inc. Methods for sequencing proteins
CA2303758C (en) * 1999-04-06 2004-01-06 Micromass Limited Improved methods of identifying peptides and proteins by mass spectrometry

Also Published As

Publication number Publication date
US6846679B1 (en) 2005-01-25
IL142314A0 (en) 2002-03-10
DE69905265D1 (de) 2003-03-13
GB9821393D0 (en) 1998-11-25
NZ510873A (en) 2002-10-25
JP3730121B2 (ja) 2005-12-21
JP2002526778A (ja) 2002-08-20
NO20011552L (no) 2001-06-01
DK1117999T3 (da) 2003-05-26
CA2344642C (en) 2004-06-22
CA2344642A1 (en) 2000-04-13
WO2000020870A1 (en) 2000-04-13
ATE232300T1 (de) 2003-02-15
AU6109699A (en) 2000-04-26
IL142314A (en) 2006-07-05
DE69905265T2 (de) 2003-11-27
NO20011552D0 (no) 2001-03-27
EP1117999A1 (en) 2001-07-25
CN1411555A (zh) 2003-04-16
PT1117999E (pt) 2003-06-30
EP1117999B1 (en) 2003-02-05
AU768902B2 (en) 2004-01-08

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