ATE232300T1 - Charakterisierung von polypeptiden durch spaltung und massenspektrometrie - Google Patents
Charakterisierung von polypeptiden durch spaltung und massenspektrometrieInfo
- Publication number
- ATE232300T1 ATE232300T1 AT99947722T AT99947722T ATE232300T1 AT E232300 T1 ATE232300 T1 AT E232300T1 AT 99947722 T AT99947722 T AT 99947722T AT 99947722 T AT99947722 T AT 99947722T AT E232300 T1 ATE232300 T1 AT E232300T1
- Authority
- AT
- Austria
- Prior art keywords
- polypeptides
- mass spectrometry
- cleavage
- polypeptide
- characterization
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 8
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 8
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 8
- 238000003776 cleavage reaction Methods 0.000 title abstract 4
- 230000007017 scission Effects 0.000 title abstract 4
- 238000004949 mass spectrometry Methods 0.000 title abstract 2
- 238000012512 characterization method Methods 0.000 title 1
- 239000012634 fragment Substances 0.000 abstract 5
- 239000003795 chemical substances by application Substances 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 2
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Genetics & Genomics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9821393.7A GB9821393D0 (en) | 1998-10-01 | 1998-10-01 | Protein profiling 2 |
| PCT/GB1999/003258 WO2000020870A1 (en) | 1998-10-01 | 1999-10-01 | Characterising polypeptides through cleavage and mass spectrometry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE232300T1 true ATE232300T1 (de) | 2003-02-15 |
Family
ID=10839813
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99947722T ATE232300T1 (de) | 1998-10-01 | 1999-10-01 | Charakterisierung von polypeptiden durch spaltung und massenspektrometrie |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US6846679B1 (de) |
| EP (1) | EP1117999B1 (de) |
| JP (1) | JP3730121B2 (de) |
| CN (1) | CN1411555A (de) |
| AT (1) | ATE232300T1 (de) |
| AU (1) | AU768902B2 (de) |
| CA (1) | CA2344642C (de) |
| DE (1) | DE69905265T2 (de) |
| DK (1) | DK1117999T3 (de) |
| ES (1) | ES2192080T3 (de) |
| GB (1) | GB9821393D0 (de) |
| IL (2) | IL142314A0 (de) |
| NO (1) | NO20011552L (de) |
| NZ (1) | NZ510873A (de) |
| PT (1) | PT1117999E (de) |
| WO (1) | WO2000020870A1 (de) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6003511A (en) | 1996-11-18 | 1999-12-21 | Medlis Corp. | Respiratory circuit terminal for a unilimb respiratory device |
| WO2001094935A2 (en) * | 2000-06-09 | 2001-12-13 | Mds Proteomics, Inc. | Labeling of proteomic samples during proteolysis for quantitation and sample multiplexing |
| AU6689401A (en) | 2000-06-12 | 2001-12-24 | Univ Washington | Selective labeling and isolation of phosphopeptides and applications to proteomeanalysis |
| GB0014342D0 (en) * | 2000-06-13 | 2000-08-02 | Scient Analysis Instr Limited | Method of analysis |
| JP2002168869A (ja) * | 2000-12-05 | 2002-06-14 | Sumitomo Chem Co Ltd | ペプチドのアミノ酸配列の決定方法 |
| JP4604345B2 (ja) * | 2000-12-21 | 2011-01-05 | 住友化学株式会社 | タンパク質のｎ末端のアミノ酸配列決定方法 |
| US20020106700A1 (en) * | 2001-02-05 | 2002-08-08 | Foote Robert S. | Method for analyzing proteins |
| DE10108892B4 (de) * | 2001-02-23 | 2005-08-18 | Graffinity Pharmaceuticals Ag | Synthesevorrichtung und Verfahren zu deren Herstellung |
| JP4163103B2 (ja) * | 2001-06-07 | 2008-10-08 | エレクトロフォレティクス リミテッド | ポリペプチドの特徴分析方法 |
| EP1267170A1 (de) * | 2001-06-07 | 2002-12-18 | Xzillion GmbH & CO.KG | Verfahren zur Charakterisierung von Polypeptiden |
| EP1265072A1 (de) * | 2001-06-07 | 2002-12-11 | Xzillion GmbH & CO.KG | Verfahren zur Charakterisierung von Polypeptiden |
| JP2005502727A (ja) * | 2001-09-21 | 2005-01-27 | カプリオン ファーマシューティカルズ インコーポレーティッド | 高度に精製された原形質膜の調製方法 |
| US7261105B2 (en) | 2001-09-24 | 2007-08-28 | F-Concepts Llc | Breathing circuits having unconventional respiratory conduits and systems and methods for optimizing utilization of fresh gases |
| US7717109B2 (en) | 2001-09-24 | 2010-05-18 | F-Concepts Llc | Breathing systems with post-inspiratory valve fresh gas flow input, components for implementing same, and methods of use |
| NZ532166A (en) | 2001-09-24 | 2007-01-26 | Atsuo F Fukunaga | Breathing circuits having unconventional respiratory conduits and systems and methods for optimising utilisation of fresh gases |
| CA2468161A1 (en) * | 2001-11-29 | 2003-06-05 | Thermo Finnigan Llc | Polypeptide quantitation |
| EP2336763A1 (de) | 2002-02-14 | 2011-06-22 | Ajinomoto Co., Inc. | Methode zur Analyse von Substanzen, welche Aminogruppen enthalten und analytische Reagenzien dafür. |
| EP1490693A1 (de) | 2002-04-04 | 2004-12-29 | Xzillion GmbH & CO.KG | Verfahren zur bestimmung von analyten |
| CN1314968C (zh) * | 2004-03-11 | 2007-05-09 | 复旦大学 | 痕量多肽或蛋白质富集及其直接分析方法 |
| US7230235B2 (en) * | 2005-05-05 | 2007-06-12 | Palo Alto Research Center Incorporated | Automatic detection of quality spectra |
| US20060249668A1 (en) * | 2005-05-05 | 2006-11-09 | Palo Alto Research Center Incorporated | Automatic detection of quality spectra |
| GB0515323D0 (en) | 2005-07-26 | 2005-08-31 | Electrophoretics Ltd | Mass labels |
| GB0518585D0 (en) | 2005-09-12 | 2005-10-19 | Electrophoretics Ltd | Mass labels |
| JP4771296B2 (ja) * | 2006-02-08 | 2011-09-14 | 日本電気株式会社 | ペプチドのｃ末端のペプチド結合を切断する方法、及びペプチドのｃ末端アミノ酸配列の決定方法 |
| EP1982991A4 (de) * | 2006-02-08 | 2009-10-21 | Nec Corp | Verfahren zur modifizierung von peptid und verfahren zur identifizierung von peptid |
| US20080319678A1 (en) * | 2006-02-15 | 2008-12-25 | University Of Virginia Patent Foundation | Mass Tagging for Quantitative Analysis of Biomolecules using 13C Labeled Phenylisocyanate |
| WO2012156764A1 (en) | 2011-05-18 | 2012-11-22 | Rudjer Boskovic Institute | Method of detection of amino acid sequence and/or identification of peptides and proteins, by use of a new derivatization reagent and synthesis of 5-formyl-benzene-1,3-disulphonic acid as derivatization reagent |
| CN111208237B (zh) * | 2018-11-21 | 2021-12-24 | 中国科学院大连化学物理研究所 | 基于肽段c末端化学衍生提高质谱碎裂效率和响应的方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2168478A (en) * | 1984-11-26 | 1986-06-18 | Scan Limited M | Analysis of protein etc using mass spectrometry |
| US6271037B1 (en) * | 1992-05-29 | 2001-08-07 | The Rockefeller University | Method and product for the sequence determination of peptides using a mass spectrometer |
| US5792664A (en) * | 1992-05-29 | 1998-08-11 | The Rockefeller University | Methods for producing and analyzing biopolymer ladders |
| US5470703A (en) * | 1992-10-21 | 1995-11-28 | Shimadzu Corporation | Method for peptide C-terminal fragment sequence analysis and apparatus for collecting peptide fragment |
| JPH06172380A (ja) * | 1992-12-04 | 1994-06-21 | Shimadzu Corp | ペプチドフラグメント分取装置 |
| WO1994028418A1 (en) * | 1993-05-28 | 1994-12-08 | Baylor College Of Medicine | Method and apparatus for desorption and ionization of analytes |
| US5538897A (en) | 1994-03-14 | 1996-07-23 | University Of Washington | Use of mass spectrometry fragmentation patterns of peptides to identify amino acid sequences in databases |
| WO1996036986A1 (en) | 1995-05-19 | 1996-11-21 | Perseptive Biosystems, Inc. | Methods and apparatus for sequencing polymers with a statistical certainty using mass spectrometry |
| US5869240A (en) * | 1995-05-19 | 1999-02-09 | Perseptive Biosystems, Inc. | Methods and apparatus for sequencing polymers with a statistical certainty using mass spectrometry |
| US5827659A (en) * | 1995-05-19 | 1998-10-27 | Perseptive Biosystems, Inc. | Methods and apparatus for sequencing polymers using mass spectrometry |
| US6245331B1 (en) * | 1997-01-02 | 2001-06-12 | New York Univ. Medical Center | Early detection of mycobacterial disease |
| ES2205437T3 (es) | 1997-01-23 | 2004-05-01 | XZILLION GMBH & CO.KG | Procedimiento que permite caracterizar polipeptidos. |
| US5824556A (en) * | 1997-06-11 | 1998-10-20 | Tarr; George E. | Peptide mass ladders generated using carbon disulfide |
| US6379971B1 (en) * | 1998-02-24 | 2002-04-30 | Target Discovery, Inc. | Methods for sequencing proteins |
| DE60031030T2 (de) * | 1999-04-06 | 2007-05-10 | Micromass UK Ltd., Simonsway | Verfahren zur Identifizierung von Peptiden und Proteinen mittels Massenspektromterie |
-
1998
- 1998-10-01 GB GBGB9821393.7A patent/GB9821393D0/en not_active Ceased
-
1999
- 1999-10-01 AT AT99947722T patent/ATE232300T1/de not_active IP Right Cessation
- 1999-10-01 AU AU61096/99A patent/AU768902B2/en not_active Ceased
- 1999-10-01 DK DK99947722T patent/DK1117999T3/da active
- 1999-10-01 PT PT99947722T patent/PT1117999E/pt unknown
- 1999-10-01 US US09/806,564 patent/US6846679B1/en not_active Expired - Fee Related
- 1999-10-01 NZ NZ510873A patent/NZ510873A/xx unknown
- 1999-10-01 WO PCT/GB1999/003258 patent/WO2000020870A1/en not_active Ceased
- 1999-10-01 IL IL14231499A patent/IL142314A0/xx not_active IP Right Cessation
- 1999-10-01 CN CN99812504.0A patent/CN1411555A/zh active Pending
- 1999-10-01 DE DE69905265T patent/DE69905265T2/de not_active Expired - Fee Related
- 1999-10-01 ES ES99947722T patent/ES2192080T3/es not_active Expired - Lifetime
- 1999-10-01 EP EP99947722A patent/EP1117999B1/de not_active Expired - Lifetime
- 1999-10-01 CA CA002344642A patent/CA2344642C/en not_active Expired - Fee Related
- 1999-10-01 JP JP2000574937A patent/JP3730121B2/ja not_active Expired - Fee Related
-
2001
- 2001-03-27 NO NO20011552A patent/NO20011552L/no not_active Application Discontinuation
- 2001-03-28 IL IL142314A patent/IL142314A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2344642A1 (en) | 2000-04-13 |
| AU6109699A (en) | 2000-04-26 |
| JP3730121B2 (ja) | 2005-12-21 |
| WO2000020870A1 (en) | 2000-04-13 |
| IL142314A (en) | 2006-07-05 |
| ES2192080T3 (es) | 2003-09-16 |
| NO20011552D0 (no) | 2001-03-27 |
| EP1117999B1 (de) | 2003-02-05 |
| CN1411555A (zh) | 2003-04-16 |
| GB9821393D0 (en) | 1998-11-25 |
| NO20011552L (no) | 2001-06-01 |
| PT1117999E (pt) | 2003-06-30 |
| CA2344642C (en) | 2004-06-22 |
| DE69905265D1 (de) | 2003-03-13 |
| NZ510873A (en) | 2002-10-25 |
| US6846679B1 (en) | 2005-01-25 |
| IL142314A0 (en) | 2002-03-10 |
| DK1117999T3 (da) | 2003-05-26 |
| JP2002526778A (ja) | 2002-08-20 |
| EP1117999A1 (de) | 2001-07-25 |
| DE69905265T2 (de) | 2003-11-27 |
| AU768902B2 (en) | 2004-01-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1117999 Country of ref document: EP |
|
| EEIH | Change in the person of patent owner | ||
| REN | Ceased due to non-payment of the annual fee |