ES2192618T3 - Metodo de seleccion. - Google Patents

Metodo de seleccion.

Info

Publication number
ES2192618T3
ES2192618T3 ES96938085T ES96938085T ES2192618T3 ES 2192618 T3 ES2192618 T3 ES 2192618T3 ES 96938085 T ES96938085 T ES 96938085T ES 96938085 T ES96938085 T ES 96938085T ES 2192618 T3 ES2192618 T3 ES 2192618T3
Authority
ES
Spain
Prior art keywords
compound
domain
fluorescence
selection method
fluorescing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96938085T
Other languages
English (en)
Inventor
Brian Arthur Hemmings
Matthias Frech
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9525703.6A external-priority patent/GB9525703D0/en
Priority claimed from GBGB9525705.1A external-priority patent/GB9525705D0/en
Application filed by Novartis AG filed Critical Novartis AG
Application granted granted Critical
Publication of ES2192618T3 publication Critical patent/ES2192618T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Eye Examination Apparatus (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

LA INVENCION TRATA DE UN PROCEDIMIENTO PARA SELECCIONAR UN COMPUESTO QUE ES UN CANDIDATO A MODULADOR DE LA RESPUESTA A UNA SEÑAL, QUE INCLUYE LAS ETAPAS DE: (A) INCUBAR EL COMPUESTO CON EL DOMINIO PH DE UNA MOLECULA SEÑALIZADORA QUE ES CAPAZ DE FLUORESCER; (B) DETERMINAR LA MODULACION INDUCIDA POR FOSFOLIPIDOS EN LA FLUORESCENCIA DEL DOMINIO PH, SIENDO UNA ALTERACION DE LA FLUORESCENCIA EN PRESENCIA DEL COMPUESTO INDICATIVA DE UNA INTERACCION FUNCIONAL ENTRE EL COMPUESTO Y EL DOMINIO PH.
ES96938085T 1995-12-15 1996-11-05 Metodo de seleccion. Expired - Lifetime ES2192618T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9525703.6A GB9525703D0 (en) 1995-12-15 1995-12-15 Screening method
GBGB9525705.1A GB9525705D0 (en) 1995-12-15 1995-12-15 Method for producing a protein

Publications (1)

Publication Number Publication Date
ES2192618T3 true ES2192618T3 (es) 2003-10-16

Family

ID=26308312

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96938085T Expired - Lifetime ES2192618T3 (es) 1995-12-15 1996-11-05 Metodo de seleccion.

Country Status (9)

Country Link
EP (1) EP0871764B1 (es)
JP (1) JP3695760B2 (es)
AT (1) ATE232911T1 (es)
AU (1) AU7564796A (es)
DE (1) DE69626310T2 (es)
DK (1) DK0871764T3 (es)
ES (1) ES2192618T3 (es)
PT (1) PT871764E (es)
WO (1) WO1997022717A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998038509A1 (en) * 1997-02-26 1998-09-03 Thyreos Corporation Drug screen targeting lipoprotein-membrane anchorage
US6461610B1 (en) 1997-07-18 2002-10-08 Novo Nordisk A/S Methods for modifying cell motility using factor VIIa or inactivated factor VIIa
ES2339196T3 (es) 1997-07-18 2010-05-17 Novo Nordisk Health Care Ag Uso de fviia o fviiai para el tratamiento de disfuncion endotelial y para la inhibicion de angiogenesis, respectivamente.
JP2002250730A (ja) * 2001-02-27 2002-09-06 Shinwa Kako Kk 分子センサー及びその製造方法

Also Published As

Publication number Publication date
EP0871764A1 (en) 1998-10-21
DE69626310T2 (de) 2003-10-16
WO1997022717A1 (en) 1997-06-26
EP0871764B1 (en) 2003-02-19
ATE232911T1 (de) 2003-03-15
JP2000502255A (ja) 2000-02-29
JP3695760B2 (ja) 2005-09-14
PT871764E (pt) 2003-06-30
DK0871764T3 (da) 2003-06-02
DE69626310D1 (de) 2003-03-27
AU7564796A (en) 1997-07-14

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