ES2194022T3 - Metodo para determinar la presencia de un organismo en una muestra fisiologica. - Google Patents

Metodo para determinar la presencia de un organismo en una muestra fisiologica.

Info

Publication number
ES2194022T3
ES2194022T3 ES94308809T ES94308809T ES2194022T3 ES 2194022 T3 ES2194022 T3 ES 2194022T3 ES 94308809 T ES94308809 T ES 94308809T ES 94308809 T ES94308809 T ES 94308809T ES 2194022 T3 ES2194022 T3 ES 2194022T3
Authority
ES
Spain
Prior art keywords
nucleic acid
probe
methods
nucleic acids
microorganism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94308809T
Other languages
English (en)
Inventor
Kathleen A Clark
Daniel L Kacian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ES2194022T3 publication Critical patent/ES2194022T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE DESCRIBEN METODOS PARA EXTRACCION DE ACIDOS NUCLEICOS POR CALENTAMIENTO DE CELULAS DE MUESTRA, POR EJ., CELULAS DE BACTERIAS, A TEMPERATURAS ENTRE 80 Y 100 REACTIVO DE PERMEABILIZACION QUE COMPRENDE UN DETERGENTE NOIONICO Y UN AGENTE DE METAL. LOS METODOS DEL INVENTO LIBERAN LARGOS FRAGMENTOS DE ACIDOS NUCLEICOS NO DEGRADADOS DE MICROORGANISMOS SIN DIVIDIR FISICAMENTE LA PARED TOTAL DE LA CELULA. SE PUEDEN DETECTAR EN MUESTRAS CLINICAS LA PRESENCIA O AUSENCIA DE UN MICROORGANISMO MEDIANTE EL EMPLEO DE UN METODO DE EXTRACCION DEL INVENTO Y AÑADIENDO SUBSECUENTEMENTE UNA SONDA DE ACIDO NUCLEICO ESPECIFICA PARA UN ACIDO NUCLEICO DEL MICROORGANISMO SELECCIONADO, INCUBANDO BAJO CONDICIONES QUE PERMITEN A LA SONDA HIBRIDAR EL MENCIONADO ACIDO NUCLEICO Y DETECTANDO SI ESTA PRESENTE CUALQUIER SONDA HIBRIDADA.
ES94308809T 1993-11-29 1994-11-29 Metodo para determinar la presencia de un organismo en una muestra fisiologica. Expired - Lifetime ES2194022T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US15894093A 1993-11-29 1993-11-29

Publications (1)

Publication Number Publication Date
ES2194022T3 true ES2194022T3 (es) 2003-11-16

Family

ID=22570371

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94308809T Expired - Lifetime ES2194022T3 (es) 1993-11-29 1994-11-29 Metodo para determinar la presencia de un organismo en una muestra fisiologica.

Country Status (11)

Country Link
US (2) US5786208A (es)
EP (1) EP0657530B1 (es)
JP (1) JP3866762B2 (es)
KR (1) KR100230909B1 (es)
AT (1) ATE234352T1 (es)
AU (1) AU693836B2 (es)
CA (1) CA2176496C (es)
DE (1) DE69432242T2 (es)
DK (1) DK0657530T3 (es)
ES (1) ES2194022T3 (es)
WO (1) WO1995014768A2 (es)

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Also Published As

Publication number Publication date
US5786208A (en) 1998-07-28
DE69432242T2 (de) 2003-08-28
JP3866762B2 (ja) 2007-01-10
WO1995014768A3 (en) 1995-08-03
ATE234352T1 (de) 2003-03-15
EP0657530A3 (en) 1995-08-30
US5837452A (en) 1998-11-17
WO1995014768A2 (en) 1995-06-01
EP0657530B1 (en) 2003-03-12
CA2176496C (en) 1999-09-28
EP0657530A2 (en) 1995-06-14
KR100230909B1 (ko) 1999-12-01
KR960705922A (ko) 1996-11-08
CA2176496A1 (en) 1995-06-01
JPH09505479A (ja) 1997-06-03
AU693836B2 (en) 1998-07-09
DK0657530T3 (da) 2003-06-10
DE69432242D1 (de) 2003-04-17
AU1183795A (en) 1995-06-13

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