ES2194903T3 - Proteina citosolica que se unea a fk-506. - Google Patents

Proteina citosolica que se unea a fk-506.

Info

Publication number
ES2194903T3
ES2194903T3 ES95910231T ES95910231T ES2194903T3 ES 2194903 T3 ES2194903 T3 ES 2194903T3 ES 95910231 T ES95910231 T ES 95910231T ES 95910231 T ES95910231 T ES 95910231T ES 2194903 T3 ES2194903 T3 ES 2194903T3
Authority
ES
Spain
Prior art keywords
protein
proline
binds
joins
binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95910231T
Other languages
English (en)
Inventor
Gregory J Wiederrecht
Tonya J Sewell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Application granted granted Critical
Publication of ES2194903T3 publication Critical patent/ES2194903T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9493—Immunosupressants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

UNA NUEVA PROTEINA DE ENLACE CITOSOLICA HOMOGENEA (FKBP12.6), QUE TIENE UNA ACTIVIDAD DE ENLACE ESPECIFICA DE ALREDEDOR DE 4,8 MG DE FK-506 POR MG DE UNA PROTEINA Y UN PESO MOLECULAR DE ALREDEDOR DE 10-12 KILODALTONS, SE UNE REVERSIBLEMENTE AL INMUNOSUPRESOR FK-506, PERO NO A LA CICLOESPONINA A (CSA). LA PROTEINA ES INESTABLE AL CALENTAMIENTO A 56 C DURANTE 30 MINUTOS PERDIENDO SU AFINIDAD DE ENLACE FK-506. LA PROTEINA FKBP12.6 ES AISLADA DEL CITOSOL DE LOS TEJIDOS DE MAMIFEROS, PREFERIBLEMENTE TEJIDO CEREBRAL BOVINO O HUMANO, Y PUEDE SER USADO EN LOS PROCEDIMIENTOS DE DIAGNOSTICO Y PURIFICACION QUE IMPLICAN INMUNOSUPRESORAS MACROLIDOS DEL TIPO FK-506. LA PROTEINA FKBP12.6 TAMBIEN TIENE ACTIVIDAD ENZIMATICA DE LA ISOMERASA PEPTIDILPROLINA, QUE CATALIZA LA CIS-TRANS ISOMERIZACION DE LOS ENLACES PEPTIDICOS QUE CONTIENEN PROLINA. ADEMAS, EL FKBP12.6 SE UNE E INHIBE LA CALCINEURINA DE FOSFATASA EN PRESENCIA DE FK-506.
ES95910231T 1994-02-15 1995-02-10 Proteina citosolica que se unea a fk-506. Expired - Lifetime ES2194903T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/197,795 US5457182A (en) 1994-02-15 1994-02-15 FK-506 cytosolic binding protein, FKBP12.6

Publications (1)

Publication Number Publication Date
ES2194903T3 true ES2194903T3 (es) 2003-12-01

Family

ID=22730799

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95910231T Expired - Lifetime ES2194903T3 (es) 1994-02-15 1995-02-10 Proteina citosolica que se unea a fk-506.

Country Status (9)

Country Link
US (1) US5457182A (es)
EP (1) EP0749443B1 (es)
JP (1) JP3747063B2 (es)
AT (1) ATE237690T1 (es)
CA (1) CA2181803A1 (es)
DE (1) DE69530378T2 (es)
DK (1) DK0749443T3 (es)
ES (1) ES2194903T3 (es)
WO (1) WO1995021861A1 (es)

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CA2042093C (en) * 1990-05-09 2002-12-24 Gyula Hadlaczky Cell line carrying an excess of mammalian centromeres
JPH07250686A (ja) * 1994-03-10 1995-10-03 Otsuka Pharmaceut Co Ltd Ｆｋ５０６結合蛋白遺伝子
MX9603838A (es) 1994-03-10 1997-03-29 Fujisawa Pharmaceutical Co Metodo para ensayar inmunosupresores.
US6187757B1 (en) 1995-06-07 2001-02-13 Ariad Pharmaceuticals, Inc. Regulation of biological events using novel compounds
JPH0924269A (ja) * 1995-07-10 1997-01-28 M Technic Kk リン脂質を使ったマイクロカプセルの製造方法
US6247995B1 (en) 1996-02-06 2001-06-19 Bruce Bryan Bioluminescent novelty items
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US6077697A (en) * 1996-04-10 2000-06-20 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US20020160970A1 (en) * 1996-04-10 2002-10-31 Gyula Hadlaczky Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6025155A (en) * 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US20030033617A1 (en) * 1996-04-10 2003-02-13 Gyula Hadlaczky Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
WO1998024886A1 (en) * 1996-12-04 1998-06-11 Brigham And Women's Hospital, Inc. Mast cell protease that cleaves fibrinogen
US5955431A (en) * 1997-02-05 1999-09-21 Brigham And Women's Hospital, Inc. Mast cell protease peptide inhibitors
CA2331382A1 (en) * 1998-06-18 1999-12-23 Curagen Corporation Interaction of p27(kip1) with fkbp-12
ES2226409T3 (es) 1998-07-17 2005-03-16 Agouron Pharmaceuticals, Inc. Compuestos, composiciones y procedimientos para estimular el crecimiento y elongacion neuronales.
DE19907598A1 (de) * 1999-02-22 2000-08-24 Schulz Burkhard DNA Sequenz kodierend ein FKBP ähnliches Protein, Plasmide, Bakterien, Hefen und Pflanzen enthaltend dieses Protein sowie Mutanten in Arabidopsis für dieses Gen, die in der Ausprägung der Pflanzenarchitektur, der Reaktion gegenüber Brassinosteroiden und ihren Verbindungen und durch Ethylen vermittelten Gravitropismus der Wurzel defekt sind
US7063857B1 (en) 1999-04-30 2006-06-20 Sucampo Ag Use of macrolide compounds for the treatment of dry eye
WO2001012790A2 (en) * 1999-08-17 2001-02-22 Incyte Genomics, Inc. Isomerase proteins
ATE264863T1 (de) * 1999-08-24 2004-05-15 Ariad Gene Therapeutics Inc 28-epirapaloge
US7067526B1 (en) 1999-08-24 2006-06-27 Ariad Gene Therapeutics, Inc. 28-epirapalogs
US7220552B1 (en) * 1999-11-19 2007-05-22 The Board Of Trustees Of The Leland Stanford Junior University Bifunctional molecules and their use in the disruption of protein-protein interactions
CN1301770A (zh) * 1999-12-29 2001-07-04 复旦大学 一种新的多肽——人fkbp蛋白11和编码这种多肽的多核苷酸
US6489335B2 (en) * 2000-02-18 2002-12-03 Gholam A. Peyman Treatment of ocular disease
US7393652B2 (en) * 2000-05-10 2008-07-01 The Trustees Of Columbia University In The City Of New York Methods for identifying a chemical compound that directly enhances binding of FKBP12.6 to PKA-phosphorylated type 2 ryanodine receptor (RyR2)
US8022058B2 (en) 2000-05-10 2011-09-20 The Trustees Of Columbia University In The City Of New York Agents for preventing and treating disorders involving modulation of the RyR receptors
US7879840B2 (en) 2005-08-25 2011-02-01 The Trustees Of Columbia University In The City Of New York Agents for preventing and treating disorders involving modulation of the RyR receptors
US7312044B2 (en) 2003-03-07 2007-12-25 The Trustees Of Columbia University In The City Of New York Type 1 ryanodine receptor-based methods
US7718644B2 (en) 2004-01-22 2010-05-18 The Trustees Of Columbia University In The City Of New York Anti-arrhythmic and heart failure drugs that target the leak in the ryanodine receptor (RyR2) and uses thereof
CN101845446B (zh) * 2000-09-28 2016-01-20 生物源食物及科学公司 脂肪酸去饱和酶家族成员fad4、fad5、fad5-2和fad6及它们的应用
CA2492153C (en) 2002-07-16 2012-05-08 Biotica Technology Limited Production of polyketide fkbp-ligand analogues
US6930127B2 (en) * 2002-08-15 2005-08-16 Eaton Veterinary Laboratories, Inc. Veterinary treatment of ophthalmic disease in animals using topical tacrolimus
US7544678B2 (en) 2002-11-05 2009-06-09 The Trustees Of Columbia University In The City Of New York Anti-arrythmic and heart failure drugs that target the leak in the ryanodine receptor (RyR2)
US7354574B2 (en) * 2002-11-07 2008-04-08 Advanced Ocular Systems Limited Treatment of ocular disease
AU2003300076C1 (en) 2002-12-30 2010-03-04 Angiotech International Ag Drug delivery from rapid gelling polymer composition
WO2004062669A1 (en) * 2003-01-16 2004-07-29 Sucampo Ag Use of a macrolide compound for treating dry eye
US7083802B2 (en) * 2003-07-31 2006-08-01 Advanced Ocular Systems Limited Treatment of ocular disease
US20050181018A1 (en) * 2003-09-19 2005-08-18 Peyman Gholam A. Ocular drug delivery
US7087237B2 (en) * 2003-09-19 2006-08-08 Advanced Ocular Systems Limited Ocular solutions
US7083803B2 (en) * 2003-09-19 2006-08-01 Advanced Ocular Systems Limited Ocular solutions
US8710045B2 (en) 2004-01-22 2014-04-29 The Trustees Of Columbia University In The City Of New York Agents for preventing and treating disorders involving modulation of the ryanodine receptors
GB0417852D0 (en) 2004-08-11 2004-09-15 Biotica Tech Ltd Production of polyketides and other natural products
US20060189551A1 (en) * 2004-10-04 2006-08-24 Duke University Combination therapies for fungal pathogens
EP1904056B1 (en) 2005-07-18 2009-04-29 Minu, L.L.C. Use of a macrolide to restore corneal sensation
US20070014760A1 (en) * 2005-07-18 2007-01-18 Peyman Gholam A Enhanced recovery following ocular surgery
US7704990B2 (en) 2005-08-25 2010-04-27 The Trustees Of Columbia University In The City Of New York Agents for preventing and treating disorders involving modulation of the RyR receptors
US7642059B2 (en) * 2005-09-07 2010-01-05 Roche Diagnostics Operations, Inc. Single receptor assays for immunosuppressive drugs
US8921642B2 (en) * 2008-01-11 2014-12-30 Massachusetts Eye And Ear Infirmary Conditional-stop dimerizable caspase transgenic animals
EP3008192B1 (en) 2013-06-11 2019-07-17 Takara Bio USA, Inc. Protein enriched microvesicles and methods of making and using the same

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3886265T2 (de) * 1987-06-05 1994-04-28 Fujisawa Pharmaceutical Co Anti-FR-900506-Stoffe-Antikörper und höchstempfindliches Enzym-Immunoassay-Verfahren.
US5109112A (en) * 1989-01-19 1992-04-28 Merck & Co., Inc. FK-506 cytosolic binding protein
WO1991004321A1 (en) * 1989-09-25 1991-04-04 President And Fellows Of Harvard College Receptor for fk-506
CA2063580C (en) * 1990-05-09 2002-11-19 Steven J. Soldin Receptor assay for fk-506
JP2982908B2 (ja) * 1990-07-11 1999-11-29 東燃株式会社 ヒトｆｋ５０６結合蛋白質をコードするｄｎａ
CA2052928A1 (en) * 1990-10-15 1992-04-16 John J. Siekierka Genes encoding the human fk-506 binding protein and a.s. cerevisiae homolog, fkb1 and their expression
AU2007192A (en) * 1991-05-08 1992-12-21 Vertex Pharmaceuticals Incorporated Rfkbp: a novel prolyl isomerase and rapamycin/fk506 binding protein
AU2803692A (en) * 1991-10-11 1993-05-03 Vertex Pharmaceuticals Incorporated Isolation of an mr 52,000 fk506 binding protein and molecular cloning of a corresponding human cdna
WO1993023548A2 (en) * 1992-05-20 1993-11-25 Vertex Pharmaceuticals Incorporated METHOD OF DETECTING TISSUE-SPECIFIC FK506 BINDING PROTEIN MESSENGER RNAs AND USES THEREOF
JPH07250686A (ja) * 1994-03-10 1995-10-03 Otsuka Pharmaceut Co Ltd Ｆｋ５０６結合蛋白遺伝子

Also Published As

Publication number Publication date
DE69530378D1 (de) 2003-05-22
EP0749443A4 (en) 1999-11-17
CA2181803A1 (en) 1995-08-17
ATE237690T1 (de) 2003-05-15
DK0749443T3 (da) 2003-06-02
JP3747063B2 (ja) 2006-02-22
EP0749443A1 (en) 1996-12-27
DE69530378T2 (de) 2003-12-18
EP0749443B1 (en) 2003-04-16
WO1995021861A1 (en) 1995-08-17
JPH09509160A (ja) 1997-09-16
US5457182A (en) 1995-10-10

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