ATE346861T1 - Verfahren zur reinigung und isolierung von viren, aus pflanzenquellen - Google Patents

Verfahren zur reinigung und isolierung von viren, aus pflanzenquellen

Info

Publication number
ATE346861T1
ATE346861T1 AT05006021T AT05006021T ATE346861T1 AT E346861 T1 ATE346861 T1 AT E346861T1 AT 05006021 T AT05006021 T AT 05006021T AT 05006021 T AT05006021 T AT 05006021T AT E346861 T1 ATE346861 T1 AT E346861T1
Authority
AT
Austria
Prior art keywords
viruses
green juice
plant
isolation
purification
Prior art date
Application number
AT05006021T
Other languages
English (en)
Inventor
Stephen J Garger
Michael J Mcculloch
R Barry Holtz
Thomas H Turpen
Original Assignee
Large Scale Biology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Large Scale Biology Corp filed Critical Large Scale Biology Corp
Application granted granted Critical
Publication of ATE346861T1 publication Critical patent/ATE346861T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/16—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/13—Immunoglobulins specific features characterized by their source of isolation or production isolated from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/00051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00051—Methods of production or purification of viral material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Detergent Compositions (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
AT05006021T 1998-03-10 1999-03-09 Verfahren zur reinigung und isolierung von viren, aus pflanzenquellen ATE346861T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/037,751 US6037456A (en) 1998-03-10 1998-03-10 Process for isolating and purifying viruses, soluble proteins and peptides from plant sources

Publications (1)

Publication Number Publication Date
ATE346861T1 true ATE346861T1 (de) 2006-12-15

Family

ID=21896116

Family Applications (2)

Application Number Title Priority Date Filing Date
AT99912327T ATE310017T1 (de) 1998-03-10 1999-03-09 Verfahren zur reinigung und isolierung von wasserlöslichen peptiden und proteinen aus pflanzenquellen
AT05006021T ATE346861T1 (de) 1998-03-10 1999-03-09 Verfahren zur reinigung und isolierung von viren, aus pflanzenquellen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT99912327T ATE310017T1 (de) 1998-03-10 1999-03-09 Verfahren zur reinigung und isolierung von wasserlöslichen peptiden und proteinen aus pflanzenquellen

Country Status (9)

Country Link
US (5) US6037456A (de)
EP (2) EP1062235B1 (de)
JP (1) JP2002506080A (de)
KR (1) KR20010034565A (de)
AT (2) ATE310017T1 (de)
AU (1) AU747647B2 (de)
CA (1) CA2322616C (de)
DE (2) DE69928379T2 (de)
WO (1) WO1999046288A2 (de)

Families Citing this family (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6846968B1 (en) * 1988-02-26 2005-01-25 Large Scale Biology Corporation Production of lysosomal enzymes in plants by transient expression
US6660500B2 (en) * 1988-02-26 2003-12-09 Large Scale Biology Corporation Production of peptides in plants as viral coat protein fusions
US7378506B2 (en) * 1997-07-21 2008-05-27 Ohio University Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins
US6639050B1 (en) 1997-07-21 2003-10-28 Ohio University Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins
US20020197249A1 (en) * 2001-04-10 2002-12-26 Renal Tech International Devices, systems, and methods for reducing levels of pro-inflammatory or anti-inflammatory stimulators or mediators in blood products
US20020164585A1 (en) * 1998-01-16 2002-11-07 Sean Chapman Method for enhancing RNA or protein production using non-native 5' untranslated sequences in recombinant viral nucleic acids
US20030027173A1 (en) * 1998-01-16 2003-02-06 Della-Cioppa Guy Method of determining the function of nucleotide sequences and the proteins they encode by transfecting the same into a host
US6426185B1 (en) 1998-01-16 2002-07-30 Large Scale Biology Corporation Method of compiling a functional gene profile in a plant by transfecting a nucleic acid sequence of a donor plant into a different host plant in an anti-sense orientation
US6303848B1 (en) 1998-01-16 2001-10-16 Large Scale Biology Corporation Method for conferring herbicide, pest, or disease resistance in plant hosts
US6906172B2 (en) 1998-03-10 2005-06-14 Large Scale Biology Corporation Flexible processing apparatus for isolating and purifying viruses, soluble proteins and peptides from plant sources
US6037456A (en) * 1998-03-10 2000-03-14 Biosource Technologies, Inc. Process for isolating and purifying viruses, soluble proteins and peptides from plant sources
US6303575B1 (en) * 1998-05-12 2001-10-16 The Regents Of The University Of California Indolicidin analogs and methods of using same
AU5142000A (en) * 1999-09-16 2001-04-17 Large Scale Biology Corporation A process for isolating and purifying viruses, soluble proteins and peptides from plant sources
US20020061309A1 (en) * 2000-03-08 2002-05-23 Garger Stephen J. Production of peptides in plants as N-terminal viral coat protein fusions
US6730306B1 (en) * 2000-03-08 2004-05-04 Large Scale Biology Corporation Parvovirus vaccine as viral coat protein fusions
JP3936661B2 (ja) 2001-01-18 2007-06-27 アイコン・ジェネティクス，インコーポレイテッド タンパク質を検出または精製するためのウィルスの使用
JP2005525081A (ja) 2001-06-05 2005-08-25 カレン ケイ． オオイシ 植物からの生物活性ミュラー管抑制物質を含むＴＧＦ−βタンパク質の遺伝子発現および産生法
EP1461620A4 (de) * 2001-12-05 2005-02-02 Large Scale Biology Corp Flexibles verfahren und gerät zur produktion und reinigung mehrerer proteine mit hohem durchsatz
US7442391B2 (en) 2002-01-25 2008-10-28 Integrated Botanical Technologies, Llc Bioactive botanical cosmetic compositions and processes for their production
US8277852B2 (en) 2002-01-25 2012-10-02 Akzo Nobel Surface Chemistry Llc Bioactive botanical cosmetic compositions and processes for their production
CA2486118A1 (en) * 2002-06-07 2003-12-18 Large Scale Biology Corporation Flexible vaccine assembly and vaccine delivery platform
US6920521B2 (en) * 2002-10-10 2005-07-19 International Business Machines Corporation Method and system of managing virtualized physical memory in a data processing system
WO2004044161A2 (en) * 2002-11-06 2004-05-27 Fraunhofer Usa Expression of foreign sequences in plants using trans-activation system
US7692063B2 (en) * 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
US7491509B2 (en) 2003-02-03 2009-02-17 Fraunhofer Usa, Inc. System for expression of genes in plants
US7683238B2 (en) * 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
ES2427641T3 (es) 2003-05-22 2013-10-31 Ibio, Inc. Molécula vehículo recombinante para la expresión, el suministro y la purificación de polipéptidos diana
PL1722805T3 (pl) * 2004-01-12 2014-08-29 Akzo Nobel Surface Chemistry Llc Biologicznie czynne kompozycje z roślin Theacea oraz sposoby ich wytwarzania i zastosowanie
EP2133427B1 (de) * 2004-01-14 2013-12-11 Ohio University Verfahren zur Herstellung von Peptiden/Proteinen in Pflanzen und damit hergestellte Peptide/Proteine
EP1769068B1 (de) 2004-02-20 2014-12-31 iBio, Inc. Systeme und verfahren zur klonalen expression in pflanzen
CA2573918A1 (en) * 2004-04-19 2005-11-24 Ohio University Cross-linkable glycoproteins and methods of making the same
US20060288449A1 (en) * 2004-10-12 2006-12-21 Garger Stephen J Process for purifying target compounds from plant sources using ceramic filtration
US7157644B2 (en) * 2004-12-16 2007-01-02 General Cable Technology Corporation Reduced alien crosstalk electrical cable with filler element
AR051853A1 (es) * 2004-12-30 2007-02-14 Schering Plough Ltd Vacuna para aumentar el crecimiento basada en epitopes neutralizantes
US20080242834A1 (en) * 2005-07-08 2008-10-02 Ohio University Methods of Predicting Hyp-Glycosylation Sites For Proteins Expressed and Secreted in Plant Cells, and Related Methods and Products
CN101378781B (zh) * 2005-08-03 2013-08-07 美国弗劳恩霍夫股份有限公司 制造免疫球蛋白的组合物和方法
WO2008048344A2 (en) * 2006-02-13 2008-04-24 Fraunhofer Usa, Inc. Bacillus anthracis antigens, vaccine compositions, and related methods
AU2007215082B2 (en) * 2006-02-13 2012-07-12 Ibio, Inc. HPV antigens, vaccine compositions, and related methods
EP1984405A4 (de) * 2006-02-13 2010-06-30 Fraunhofer Usa Inc Influenza-antigene, impfstoffzusammensetzungen und zugehörige verfahren
GB0605684D0 (en) * 2006-03-21 2006-05-03 Sicor Biotech Uab Method For Purifying Granulocyte-Colony Stimulating Factor
CN102026645B (zh) 2006-09-15 2016-01-27 渥太华医院研究机构 溶瘤弹状病毒
WO2008100578A2 (en) * 2007-02-14 2008-08-21 Amgen Inc. Method of isolating antibodies by precipitation
US8778348B2 (en) * 2007-04-28 2014-07-15 Ibio Inc. Trypanosoma antigens, vaccine compositions, and related methods
US8404252B2 (en) 2007-07-11 2013-03-26 Fraunhofer Usa, Inc. Yersinia pestis antigens, vaccine compositions, and related methods
CA2696764A1 (en) * 2007-08-20 2009-02-26 Fraunhofer Usa, Inc. Prophylactic and therapeutic influenza vaccines, antigens, compositions, and methods
US8734803B2 (en) 2008-09-28 2014-05-27 Ibio Inc. Humanized neuraminidase antibody and methods of use thereof
EP2483307A1 (de) 2009-09-29 2012-08-08 Fraunhofer USA, Inc. Influenza-hämagglutinin-antikörper, zusammensetzungen daraus und zugehörige verfahren
JP2013523175A (ja) 2010-04-14 2013-06-17 イーエムディー・ミリポア・コーポレーション 高力価、高純度のウイルスストックの作製方法及びその使用方法
US9220295B2 (en) * 2010-12-01 2015-12-29 R.J. Reynolds Tobacco Company Tobacco separation process for extracting tobacco-derived materials, and associated extraction systems
EP3219313B1 (de) * 2011-04-18 2020-10-28 National Center of Neurology and Psychiatry Arzneimittelabgabepartikel und verfahren zur herstellung davon
US9315831B2 (en) 2012-03-30 2016-04-19 Danisco Us Inc. Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids
WO2013148207A2 (en) 2012-03-30 2013-10-03 Danisco Us Inc. Direct starch to fermentable sugar
US9301544B2 (en) 2013-03-14 2016-04-05 R.J. Reynolds Tobacco Company Protein-enriched tobacco-derived composition
US9175052B2 (en) * 2013-05-17 2015-11-03 R.J. Reynolds Tobacco Company Tobacco-derived protein compositions
CN103543264B (zh) * 2013-11-05 2015-10-07 广西大学 淮山药x病毒的多克隆抗体制备、检测及其应用
PL3201336T3 (pl) 2014-10-01 2021-12-13 Impossible Foods Inc. Sposoby ekstrakcji i oczyszczania białek niezdenaturowanych
US11124848B2 (en) * 2015-07-17 2021-09-21 Nunhems B.V. Species of tobamovirus
US11612183B2 (en) 2015-12-10 2023-03-28 R.J. Reynolds Tobacco Company Protein-enriched tobacco composition
US20170165252A1 (en) 2015-12-10 2017-06-15 Niconovum Usa Inc. Protein-enriched therapeutic composition
US10499684B2 (en) 2016-01-28 2019-12-10 R.J. Reynolds Tobacco Company Tobacco-derived flavorants
US10155176B1 (en) 2016-11-03 2018-12-18 Healer, LLC Process for the production of a concentrated cannabinoid product
US11091446B2 (en) 2017-03-24 2021-08-17 R.J. Reynolds Tobacco Company Methods of selectively forming substituted pyrazines
US20180362957A1 (en) 2017-06-14 2018-12-20 R. J. Reynolds Tobacco Company RuBisCO Protein-Based Films
US10745682B2 (en) 2017-06-14 2020-08-18 R.J. Reynolds Tobacco Company Method of producing RuBisCO protein fibers
US10757964B2 (en) 2017-07-20 2020-09-01 R.J. Reynolds Tobacco Company Purification of tobacco-derived protein compositions
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
US11529413B2 (en) 2018-06-12 2022-12-20 Kbio Holdings Limited Virus and antigen purification and conjugation
US11690907B2 (en) 2018-06-12 2023-07-04 Kbio Holdings Limited Vaccines formed by virus and antigen conjugation
US11696948B2 (en) 2018-06-12 2023-07-11 Kbio Holdings Limited Vaccines formed by virus and antigen conjugation
AU2019286406B2 (en) 2018-06-12 2023-04-13 Kbio Holdings Limited Virus and antigen purification and conjugation
US11523623B2 (en) 2019-01-18 2022-12-13 R.J. Reynolds Tobacco Company Plant-derived protein purification
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions
WO2023039419A2 (en) 2021-09-08 2023-03-16 Plantible Foods Inc. Systems and methods for measuring mat density of aquatic biomass
CN116284476B (zh) * 2023-02-24 2025-05-16 复旦大学 鸭跖草多糖及其制备方法与应用

Family Cites Families (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2593540A (en) * 1945-09-01 1952-04-22 American Viscose Corp Semipermeable membrane
US2610932A (en) * 1950-07-27 1952-09-16 Laval Separator Co De Clarification of sugar cane juice
BE608328A (de) * 1960-09-19
GB1128336A (en) * 1964-09-28 1968-09-25 Budapesti Mueszaki Egyetem Plant extract
US3342729A (en) * 1964-12-09 1967-09-19 Dow Chemical Co Permeability separatory cell and apparatus and method of using the same
US4083732A (en) * 1965-01-11 1978-04-11 Paley Lewis A Sugar juice treatment
US3298865A (en) * 1966-04-18 1967-01-17 Bode Harold Eli Crude sugar liquor defecation process
GB1350261A (en) * 1970-10-16 1974-04-18 Hitachi Shipbuilding Eng Co Sugar refining process
SU654149A3 (ru) 1971-05-28 1979-03-25 Kohler George O Способ получени протеиновой кормовой добавки из зеленой массы
US3914410A (en) * 1972-06-13 1975-10-21 Bristol Myers Co Method for preparing sucrose composition containing chromium III salt
ZA752191B (en) * 1974-04-17 1976-11-24 Dorr Oliver Inc Treatment of raw sugar juice
US4006078A (en) 1974-07-08 1977-02-01 The United States Of America As Represented By The Secretary Of Agriculture Preparation of soluble edible protein from leafy green crops
JPS5912278B2 (ja) * 1976-04-01 1984-03-22 三井製糖株式会社 甘庶汁から栄養糖を製造する方法
US4006633A (en) 1976-04-22 1977-02-08 Bethlehem Steel Corporation Method and apparatus for determining heat removal from a continuous caster
HU174943B (hu) * 1976-06-30 1980-04-28 Vepex Foevallalkozasi Iroda Rt Sposob poluchenija koncentratov rastitel'nogo belka i pigmenta
US4156618A (en) * 1976-09-07 1979-05-29 Asahi-Dow Limited Process for separating ketoses and aldoses
CA1028552A (en) * 1976-09-30 1978-03-28 General Foods Protein product and process for preparing same
US4250197A (en) * 1976-10-25 1981-02-10 Vepex Fovallalkopasi Iroda Rt. Method for processing plant protein
US4130553A (en) * 1977-01-05 1978-12-19 Batley Jr William R Process for improving the nutritional value of green plant protein
US4234350A (en) * 1979-05-07 1980-11-18 Davies Hamakua Sugar Co., A Division Of Theo. H. Davies, Ltd. Process for the purification of evaporated sugar solutions
US4347324A (en) * 1979-09-24 1982-08-31 Leaf Proteins, Inc. Process for isolation of proteins from plant leaves
US4268632A (en) * 1979-09-24 1981-05-19 Leaf Proteins, Inc. Process for isolation of ribulose 1,5-diphosphate carboxylase from plant leaves
US4289147A (en) * 1979-11-15 1981-09-15 Leaf Proteins, Inc. Process for obtaining deproteinized tobacco freed of nicotine and green pigment, for use as a smoking product
US4359530A (en) * 1980-09-08 1982-11-16 Aluminum Company Of America Protein extraction from green crops
FR2490676B1 (fr) * 1980-09-19 1985-07-19 Rhone Poulenc Spec Chim Procede d'epuration des jus de canne a sucre
US4334024A (en) * 1980-11-03 1982-06-08 Sarjit Johal Preparation and crystallization of fraction I protein from plant sources
US4400471A (en) * 1980-11-03 1983-08-23 Johal Sarjit S Preparation and crystallization of Fraction I protein from plant sources
US4396763A (en) * 1981-01-14 1983-08-02 Meiji Milk Products Company Limited High molecular polysaccharide MPS-80
FI61988C (fi) * 1981-01-28 1982-11-10 Vehnae Ab Oy Foerfarande foer fraktionering av spannmaolsmjoel till fraktioner av livsmedelskvalitet
SU946488A1 (ru) * 1981-02-27 1982-07-30 Центральный Научно-Исследовательский И Проектно-Технологический Институт Механизации И Электрификации Животноводства Южной Зоны Ссср Способ получени протеинового концентрата из сока зеленых растений
US4370267A (en) * 1981-08-10 1983-01-25 A. E. Staley Manufacturing Company Fractionation and isolation of 7S and 11S protein from isoelectrically precipitated vegetable protein mixtures
US4430849A (en) 1981-11-03 1984-02-14 Powell Manufacturing Co. Harvester for tea or the like
US5320953A (en) * 1982-08-12 1994-06-14 Howard Florey Institute Of Experimental Physiology And Medicine Process for synthesizing human H1-prorelaxin, H1-relaxin and fusion proteins thereof
US4963356A (en) * 1982-10-13 1990-10-16 Minnesota Mining And Manufacturing Company Stable antigenic extracts methods
US4885248A (en) * 1984-02-15 1989-12-05 Lubrizol Genetics, Inc. Transfer vector
US5173410A (en) * 1984-02-15 1992-12-22 Lubrizol Genetics Inc. Transfer vector
DE3407364A1 (de) * 1984-02-29 1985-08-29 Pfeifer & Langen, 5000 Köln Naturbelassener vollrohrzucker und verfahren zu seiner herstellung
CA1288073C (en) * 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US5466788A (en) * 1985-03-07 1995-11-14 Mycogen Plant Science, Inc. Subgenomic promoter
JPS61212217A (ja) 1985-03-18 1986-09-20 日本たばこ産業株式会社 タバコ葉収穫用脱葉装置
US5597945A (en) * 1986-07-25 1997-01-28 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Plants genetically enhanced for disease resistance
ATE108828T1 (de) * 1987-02-09 1994-08-15 Lubrizol Genetics Inc Hybrides rns-virus.
US5316931A (en) * 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US4950332A (en) * 1988-03-17 1990-08-21 The Dow Chemical Company Process for decolorizing aqueous sugar solutions via adsorbent resins, and desorption of color bodies from the adsorbent resins
US4871397A (en) * 1988-05-09 1989-10-03 The Dow Chemical Company Process for decolorizing aqueous sugar solution
GB2221696B (en) * 1988-07-15 1991-10-02 Itoh Sugar Co Ltd C Method for refining sugar liquor
US5498544A (en) 1988-11-10 1996-03-12 Regents Of The University Of Minnesota Method and an acetyl CoA carboxylase gene for conferring herbicide tolerance
US5077390A (en) * 1989-04-04 1991-12-31 Genelabs, Incorporated Purified trichosanthin and method of preparation
PT92072B (pt) * 1989-10-23 1995-06-30 Rar Refinarias Acucar Reunidas Processo para descoloracao e descalcificacao de solucoes de acucar
US5281279A (en) * 1991-11-04 1994-01-25 Gil Enrique G Process for producing refined sugar from raw juices
US5301694A (en) * 1991-11-12 1994-04-12 Philip Morris Incorporated Process for isolating plant extract fractions
CA2140891A1 (en) * 1992-07-24 1994-02-03 David C. Parmelee Peptides useful as internal standards for microsequencing and methods for their use
JP2787539B2 (ja) * 1993-02-26 1998-08-20 松森　　昭 ウイルス性疾患の予防または治療剤
FR2707997B1 (fr) * 1993-07-19 1995-09-29 Applexion Ste Nle Rech Applic Procédé de raffinage d'un sucre brut, notamment de sucre roux provenant de l'industrie sucrière de la canne à sucre.
US5554227A (en) * 1993-11-12 1996-09-10 Societe Nouvelle De Recherches Et D'applications Industrielles D'echangeurs D'ions Applexion Process of manufacturing crystal sugar from an aqueous sugar juice such as cane juice or sugar beet juice
US5693506A (en) * 1993-11-16 1997-12-02 The Regents Of The University Of California Process for protein production in plants
US5468300A (en) * 1994-04-07 1995-11-21 International Food Processing Incorporated Process for producing refined sugar directly from sugarcane
US6096136A (en) * 1996-10-18 2000-08-01 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Method for producing white sugar
RU2114177C1 (ru) * 1997-06-02 1998-06-27 Товарищество с ограниченной ответственностью Центр содействия развитию новых технологий "Кантэк" Способ производства сахарного сиропа из сахаросодержащего сырья
US6037456A (en) * 1998-03-10 2000-03-14 Biosource Technologies, Inc. Process for isolating and purifying viruses, soluble proteins and peptides from plant sources
JPH11313634A (ja) * 1998-03-12 1999-11-16 Internatl Flavors & Fragrances Inc <Iff> 呈味剤とその製造方法
US6194636B1 (en) * 1999-05-14 2001-02-27 Dekalb Genetics Corp. Maize RS324 promoter and methods for use thereof
US6174378B1 (en) * 1999-08-19 2001-01-16 Tate Life Industries, Limited Process for production of extra low color cane sugar
US6730306B1 (en) * 2000-03-08 2004-05-04 Large Scale Biology Corporation Parvovirus vaccine as viral coat protein fusions
US6479636B1 (en) 2000-04-11 2002-11-12 Honiron Corporation (A Louisiana Corporation) Sugarcane fractioning system
WO2002083715A1 (en) 2001-04-10 2002-10-24 Honiron Corporation Method and apparatus for fractional separation of proteins from plant material

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AU3072599A (en) 1999-09-27
DE69928379T2 (de) 2006-08-03
DE69928379D1 (de) 2005-12-22
AU747647B2 (en) 2002-05-16
WO1999046288A3 (en) 2000-01-20
EP1062235B1 (de) 2005-11-16
US20040171813A1 (en) 2004-09-02
CA2322616A1 (en) 1999-09-16
US6037456A (en) 2000-03-14
EP1561758A1 (de) 2005-08-10
DE69934269D1 (de) 2007-01-11
JP2002506080A (ja) 2002-02-26
EP1062235A2 (de) 2000-12-27
CA2322616C (en) 2008-05-27
US20030049813A1 (en) 2003-03-13
ATE310017T1 (de) 2005-12-15
EP1561758B1 (de) 2006-11-29
US6740740B2 (en) 2004-05-25
KR20010034565A (ko) 2001-04-25
US6033895A (en) 2000-03-07
DE69934269T2 (de) 2007-09-20
US6303779B1 (en) 2001-10-16
WO1999046288A2 (en) 1999-09-16

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