ES2200705A1 - Elemento regulador que activa la expresion genica en condiciones de baja tension de oxigeno y represion por glucosa. - Google Patents
Elemento regulador que activa la expresion genica en condiciones de baja tension de oxigeno y represion por glucosa.Info
- Publication number
- ES2200705A1 ES2200705A1 ES200201930A ES200201930A ES2200705A1 ES 2200705 A1 ES2200705 A1 ES 2200705A1 ES 200201930 A ES200201930 A ES 200201930A ES 200201930 A ES200201930 A ES 200201930A ES 2200705 A1 ES2200705 A1 ES 2200705A1
- Authority
- ES
- Spain
- Prior art keywords
- regulatory element
- gene expression
- glucose
- low voltage
- oxygen conditions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000014509 gene expression Effects 0.000 title abstract 5
- 230000001105 regulatory effect Effects 0.000 title abstract 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title abstract 4
- 229910052760 oxygen Inorganic materials 0.000 title abstract 4
- 239000001301 oxygen Substances 0.000 title abstract 4
- 230000012666 negative regulation of transcription by glucose Effects 0.000 title 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 3
- 239000008103 glucose Substances 0.000 abstract 3
- 239000001963 growth medium Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Elemento regulador que activa la expresión génica en condiciones de baja tensión de oxígeno y represión por glucosa y aplicaciones. El elemento regulador activa la expresión del gen al que está operativamente fusionado en condiciones de baja tensión de oxígeno y alta concentración de glucosa en el medio de cultivo. Dicho elemento regulador puede utilizarse para construir cassettes de expresión, vectores y sistemas de expresión eucariotas susceptibles de ser regulados por acción del oxígeno y de no ser reprimidos por glucosa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200201930A ES2200705B1 (es) | 2002-08-14 | 2002-08-14 | Elemento regulador que activa la expresion genica en condiciones de baja tension de oxigeno y represion por glucosa y aplicaciones. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200201930A ES2200705B1 (es) | 2002-08-14 | 2002-08-14 | Elemento regulador que activa la expresion genica en condiciones de baja tension de oxigeno y represion por glucosa y aplicaciones. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2200705A1 true ES2200705A1 (es) | 2004-03-01 |
| ES2200705B1 ES2200705B1 (es) | 2005-04-16 |
Family
ID=31970624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200201930A Expired - Lifetime ES2200705B1 (es) | 2002-08-14 | 2002-08-14 | Elemento regulador que activa la expresion genica en condiciones de baja tension de oxigeno y represion por glucosa y aplicaciones. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2200705B1 (es) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR9202983A (pt) * | 1992-07-31 | 1994-02-01 | Univ Sao Paulo | Processo para melhoramento de producao de celulases atraves da aceleracao da inducao dos transcritos de celobiohidrolase i(cbh 1)e endoglucanase i(egl 1)em fungos celuloliticos |
| WO1994004673A1 (en) * | 1992-08-19 | 1994-03-03 | Alko Group Ltd. | Fungal promoters active in the presence of glucose |
| EP0814162A2 (en) * | 1996-06-21 | 1997-12-29 | Hoechst Aktiengesellschaft | Expression of the alpha-toxin gene (hla) of staphylococcus aureus using a chromosomally encoded hla-lacZ gene fusion |
| US5830692A (en) * | 1995-03-24 | 1998-11-03 | Consortium Fur Elektrochemische Industrie Gmbh | Expression system which can be regulated |
-
2002
- 2002-08-14 ES ES200201930A patent/ES2200705B1/es not_active Expired - Lifetime
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR9202983A (pt) * | 1992-07-31 | 1994-02-01 | Univ Sao Paulo | Processo para melhoramento de producao de celulases atraves da aceleracao da inducao dos transcritos de celobiohidrolase i(cbh 1)e endoglucanase i(egl 1)em fungos celuloliticos |
| WO1994004673A1 (en) * | 1992-08-19 | 1994-03-03 | Alko Group Ltd. | Fungal promoters active in the presence of glucose |
| US5830692A (en) * | 1995-03-24 | 1998-11-03 | Consortium Fur Elektrochemische Industrie Gmbh | Expression system which can be regulated |
| EP0814162A2 (en) * | 1996-06-21 | 1997-12-29 | Hoechst Aktiengesellschaft | Expression of the alpha-toxin gene (hla) of staphylococcus aureus using a chromosomally encoded hla-lacZ gene fusion |
Non-Patent Citations (2)
| Title |
|---|
| ABRAHAO-NETO J et al. Mitochondrial Functions Mediate Cellulase Gene Expression in Trichoderma reesei. Biochemistry, 1995, Vol. 34, Nº 33, paginas 10456-10462, figura 3B. * |
| CHAMBERGO FS et al. Elucidation of the metabolic fate of glucose in the filamentous fungus Trichoderma reesei using expressed sequence tag (EST) analysis and CDNA microarrays. J. Biol. Chem., Abril 2002, Vol. 277, Nº 16, paginas 13983-8. * |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2200705B1 (es) | 2005-04-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20040301 Kind code of ref document: A1 |
|
| FG2A | Definitive protection |
Ref document number: 2200705B1 Country of ref document: ES |
|
| FA2A | Application withdrawn |
Effective date: 20051117 |