ES2237184T3 - Deposicion catalizada de mensajeros potenciada. - Google Patents

Deposicion catalizada de mensajeros potenciada.

Info

Publication number: ES2237184T3
Authority: ES; Spain
Prior art keywords: inorganic salt; cooh; compound; consisting essentially; group
Prior art date: 1998-11-09
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

ES99958831T

Other languages

English (en)

Spanish (es)

Inventor

Mark Norman Bobrow

Karl Edwin Adler

Kevin Aaron Roth

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Revvity Health Sciences Inc

Original Assignee

NEN Life Science Products Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-11-09

Filing date

1999-11-08

Publication date

2005-07-16

1999-11-08 Application filed by NEN Life Science Products Inc filed Critical NEN Life Science Products Inc

2005-07-16 Application granted granted Critical

2005-07-16 Publication of ES2237184T3 publication Critical patent/ES2237184T3/es

2019-11-08 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

230000008021 deposition Effects 0.000 title claims description 13
239000000758 substrate Substances 0.000 claims abstract description 40
239000003153 chemical reaction reagent Substances 0.000 claims abstract description 25
238000000034 method Methods 0.000 claims abstract description 20
102000003992 Peroxidases Human genes 0.000 claims abstract description 14
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims abstract description 14
150000001875 compounds Chemical class 0.000 claims abstract description 14
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims abstract description 13
229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 12
238000006243 chemical reaction Methods 0.000 claims abstract description 9
150000002894 organic compounds Chemical class 0.000 claims abstract description 8
229910052799 carbon Inorganic materials 0.000 claims abstract description 6
125000004432 carbon atom Chemical group C* 0.000 claims abstract description 6
229910052736 halogen Inorganic materials 0.000 claims abstract description 6
150000002367 halogens Chemical class 0.000 claims abstract description 6
125000005842 heteroatom Chemical group 0.000 claims abstract description 6
239000000203 mixture Substances 0.000 claims abstract description 6
229910052760 oxygen Inorganic materials 0.000 claims abstract description 6
229910052717 sulfur Inorganic materials 0.000 claims abstract description 6
125000001424 substituent group Chemical group 0.000 claims abstract description 5
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims abstract description 4
239000003623 enhancer Substances 0.000 claims description 30
235000002639 sodium chloride Nutrition 0.000 claims description 21
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
125000000217 alkyl group Chemical group 0.000 claims description 14
WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 10
239000011780 sodium chloride Substances 0.000 claims description 8
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 6
239000001103 potassium chloride Substances 0.000 claims description 5
230000003321 amplification Effects 0.000 claims description 4
238000003199 nucleic acid amplification method Methods 0.000 claims description 4
108040007629 peroxidase activity proteins Proteins 0.000 claims description 4
USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims description 3
239000005695 Ammonium acetate Substances 0.000 claims description 3
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
229940043376 ammonium acetate Drugs 0.000 claims description 3
235000019257 ammonium acetate Nutrition 0.000 claims description 3
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
239000001110 calcium chloride Substances 0.000 claims description 3
229910001628 calcium chloride Inorganic materials 0.000 claims description 3
235000011148 calcium chloride Nutrition 0.000 claims description 3
229910001629 magnesium chloride Inorganic materials 0.000 claims description 3
235000011147 magnesium chloride Nutrition 0.000 claims description 3
239000001488 sodium phosphate Substances 0.000 claims description 3
229910000162 sodium phosphate Inorganic materials 0.000 claims description 3
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 3
-1 tyramine compound Chemical class 0.000 claims description 3
239000001632 sodium acetate Substances 0.000 claims description 2
235000017281 sodium acetate Nutrition 0.000 claims description 2
108010001336 Horseradish Peroxidase Proteins 0.000 claims 2
230000002378 acidificating effect Effects 0.000 claims 2
DZGWFCGJZKJUFP-UHFFFAOYSA-N Tyramine Natural products NCCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-N 0.000 claims 1
239000003054 catalyst Substances 0.000 claims 1
230000002255 enzymatic effect Effects 0.000 claims 1
229960003732 tyramine Drugs 0.000 claims 1
230000002708 enhancing effect Effects 0.000 abstract description 5
229910052757 nitrogen Inorganic materials 0.000 abstract description 2
125000001626 borono group Chemical group [H]OB([*])O[H] 0.000 abstract 1
238000001514 detection method Methods 0.000 description 40
102100034763 Peroxiredoxin-2 Human genes 0.000 description 34
239000000047 product Substances 0.000 description 19
QGKMIGUHVLGJBR-UHFFFAOYSA-M (4z)-1-(3-methylbutyl)-4-[[1-(3-methylbutyl)quinolin-1-ium-4-yl]methylidene]quinoline;iodide Chemical compound [I-].C12=CC=CC=C2N(CCC(C)C)C=CC1=CC1=CC=[N+](CCC(C)C)C2=CC=CC=C12 QGKMIGUHVLGJBR-UHFFFAOYSA-M 0.000 description 17
230000003647 oxidation Effects 0.000 description 17
238000007254 oxidation reaction Methods 0.000 description 17
101710185562 Peroxiredoxin 2 Proteins 0.000 description 15
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 14
150000003839 salts Chemical class 0.000 description 13
238000000151 deposition Methods 0.000 description 12
RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 12
230000027455 binding Effects 0.000 description 10
239000000872 buffer Substances 0.000 description 8
230000000694 effects Effects 0.000 description 8
239000012634 fragment Substances 0.000 description 8
239000007976 Tris-NaCl-Tween buffer Substances 0.000 description 7
102000004190 Enzymes Human genes 0.000 description 6
108090000790 Enzymes Proteins 0.000 description 6
STOOUUMSJPLRNI-UHFFFAOYSA-N 5-amino-4-hydroxy-3-[[4-[4-[(4-hydroxyphenyl)diazenyl]phenyl]phenyl]diazenyl]-6-[(4-nitrophenyl)diazenyl]naphthalene-2,7-disulfonic acid Chemical compound OS(=O)(=O)C1=CC2=CC(S(O)(=O)=O)=C(N=NC=3C=CC(=CC=3)C=3C=CC(=CC=3)N=NC=3C=CC(O)=CC=3)C(O)=C2C(N)=C1N=NC1=CC=C([N+]([O-])=O)C=C1 STOOUUMSJPLRNI-UHFFFAOYSA-N 0.000 description 5
SXEHKFHPFVVDIR-UHFFFAOYSA-N [4-(4-hydrazinylphenyl)phenyl]hydrazine Chemical compound C1=CC(NN)=CC=C1C1=CC=C(NN)C=C1 SXEHKFHPFVVDIR-UHFFFAOYSA-N 0.000 description 5
239000000427 antigen Substances 0.000 description 5
102000036639 antigens Human genes 0.000 description 5
108091007433 antigens Proteins 0.000 description 5
238000006555 catalytic reaction Methods 0.000 description 4
KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 4
210000002307 prostate Anatomy 0.000 description 4
230000009870 specific binding Effects 0.000 description 4
KGLPWQKSKUVKMJ-UHFFFAOYSA-N 2,3-dihydrophthalazine-1,4-dione Chemical compound C1=CC=C2C(=O)NNC(=O)C2=C1 KGLPWQKSKUVKMJ-UHFFFAOYSA-N 0.000 description 3
241000283973 Oryctolagus cuniculus Species 0.000 description 3
239000002253 acid Substances 0.000 description 3
239000003085 diluting agent Substances 0.000 description 3
239000007790 solid phase Substances 0.000 description 3
210000001519 tissue Anatomy 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
PHOLIFLKGONSGY-CSKARUKUSA-N (e)-(3-methyl-1,3-benzothiazol-2-ylidene)hydrazine Chemical compound C1=CC=C2S\C(=N\N)N(C)C2=C1 PHOLIFLKGONSGY-CSKARUKUSA-N 0.000 description 2
GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 2
YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 2
LVSPDZAGCBEQAV-UHFFFAOYSA-N 4-chloronaphthalen-1-ol Chemical compound C1=CC=C2C(O)=CC=C(Cl)C2=C1 LVSPDZAGCBEQAV-UHFFFAOYSA-N 0.000 description 2
NGSWKAQJJWESNS-UHFFFAOYSA-N 4-coumaric acid Chemical compound OC(=O)C=CC1=CC=C(O)C=C1 NGSWKAQJJWESNS-UHFFFAOYSA-N 0.000 description 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
241000147041 Guaiacum officinale Species 0.000 description 2
WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
230000004913 activation Effects 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
229940091561 guaiac Drugs 0.000 description 2
LHGVFZTZFXWLCP-UHFFFAOYSA-N guaiacol Chemical compound COC1=CC=CC=C1O LHGVFZTZFXWLCP-UHFFFAOYSA-N 0.000 description 2
230000001900 immune effect Effects 0.000 description 2
230000002163 immunogen Effects 0.000 description 2
239000012120 mounting media Substances 0.000 description 2
150000002989 phenols Chemical class 0.000 description 2
102000004169 proteins and genes Human genes 0.000 description 2
108090000623 proteins and genes Proteins 0.000 description 2
239000007787 solid Substances 0.000 description 2
PELJYVULHLKXFF-UHFFFAOYSA-N (4-iodophenyl)boronic acid Chemical compound OB(O)C1=CC=C(I)C=C1 PELJYVULHLKXFF-UHFFFAOYSA-N 0.000 description 1
150000003923 2,5-pyrrolediones Chemical class 0.000 description 1
NEGFNJRAUMCZMY-UHFFFAOYSA-N 3-(dimethylamino)benzoic acid Chemical compound CN(C)C1=CC=CC(C(O)=O)=C1 NEGFNJRAUMCZMY-UHFFFAOYSA-N 0.000 description 1
VZWXNOBHWODXCW-ZOBUZTSGSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-n-[2-(4-hydroxyphenyl)ethyl]pentanamide Chemical compound C1=CC(O)=CC=C1CCNC(=O)CCCC[C@H]1[C@H]2NC(=O)N[C@H]2CS1 VZWXNOBHWODXCW-ZOBUZTSGSA-N 0.000 description 1
ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
235000011330 Armoracia rusticana Nutrition 0.000 description 1
240000003291 Armoracia rusticana Species 0.000 description 1
WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
102000014914 Carrier Proteins Human genes 0.000 description 1
ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
239000007832 Na2SO4 Substances 0.000 description 1
108700020962 Peroxidase Proteins 0.000 description 1
PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
239000000654 additive Substances 0.000 description 1
150000001412 amines Chemical class 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
125000003118 aryl group Chemical group 0.000 description 1
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical group [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
108091008324 binding proteins Proteins 0.000 description 1
125000004057 biotinyl group Chemical group [H]N1C(=O)N([H])[C@]2([H])[C@@]([H])(SC([H])([H])[C@]12[H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C(*)=O 0.000 description 1
230000000903 blocking effect Effects 0.000 description 1
GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
229910052794 bromium Inorganic materials 0.000 description 1
125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
230000003197 catalytic effect Effects 0.000 description 1
239000003638 chemical reducing agent Substances 0.000 description 1
229910052801 chlorine Inorganic materials 0.000 description 1
239000000460 chlorine Substances 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
239000012084 conversion product Substances 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
239000000975 dye Substances 0.000 description 1
238000007824 enzymatic assay Methods 0.000 description 1
125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
229910052731 fluorine Inorganic materials 0.000 description 1
239000011737 fluorine Substances 0.000 description 1
229940014144 folate Drugs 0.000 description 1
239000011724 folic acid Substances 0.000 description 1
229960001867 guaiacol Drugs 0.000 description 1
125000004051 hexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
230000001744 histochemical effect Effects 0.000 description 1
125000001183 hydrocarbyl group Chemical group 0.000 description 1
229910052739 hydrogen Inorganic materials 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
150000002431 hydrogen Chemical class 0.000 description 1
239000000852 hydrogen donor Substances 0.000 description 1
239000003112 inhibitor Substances 0.000 description 1
239000011630 iodine Substances 0.000 description 1
229910052740 iodine Inorganic materials 0.000 description 1
125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 description 1
125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
150000002540 isothiocyanates Chemical class 0.000 description 1
239000006166 lysate Substances 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
239000001301 oxygen Substances 0.000 description 1
125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 1
239000002953 phosphate buffered saline Substances 0.000 description 1
235000011164 potassium chloride Nutrition 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
230000009257 reactivity Effects 0.000 description 1
239000000523 sample Substances 0.000 description 1
125000002914 sec-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
239000011734 sodium Substances 0.000 description 1
235000011008 sodium phosphates Nutrition 0.000 description 1
229910052938 sodium sulfate Inorganic materials 0.000 description 1
235000011152 sodium sulphate Nutrition 0.000 description 1
238000010186 staining Methods 0.000 description 1
239000000126 substance Substances 0.000 description 1
239000011593 sulfur Substances 0.000 description 1
125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
125000003396 thiol group Chemical group [H]S* 0.000 description 1
125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C235/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms
- C07C235/02—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton
- C07C235/04—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton being acyclic and saturated
- C07C235/18—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton being acyclic and saturated having at least one of the singly-bound oxygen atoms further bound to a carbon atom of a six-membered aromatic ring, e.g. phenoxyacetamides
- C07C235/20—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton being acyclic and saturated having at least one of the singly-bound oxygen atoms further bound to a carbon atom of a six-membered aromatic ring, e.g. phenoxyacetamides having the nitrogen atoms of the carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Immunology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Analytical Chemistry (AREA)
Biotechnology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Inert Electrodes (AREA)
Carbon And Carbon Compounds (AREA)
Reinforced Plastic Materials (AREA)
Luminescent Compositions (AREA)

ES99958831T 1998-11-09 1999-11-08 Deposicion catalizada de mensajeros potenciada. Expired - Lifetime ES2237184T3 (es)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
US10765498P	1998-11-09	1998-11-09
US107654P		1998-11-09
US434742		1999-11-05
US09/434,742 US6372937B1 (en)	1998-11-09	1999-11-05	Enhanced catalyzed reporter deposition

Publications (1)

Publication Number	Publication Date
ES2237184T3 true ES2237184T3 (es)	2005-07-16

Family

ID=26805004

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ES99958831T Expired - Lifetime ES2237184T3 (es)	1998-11-09	1999-11-08	Deposicion catalizada de mensajeros potenciada.

Country Status (8)

Country	Link
US (2)	US6372937B1 (de)
EP (1)	EP1129214B1 (de)
AT (1)	ATE288497T1 (de)
AU (1)	AU768695B2 (de)
CA (1)	CA2351579C (de)
DE (1)	DE69923580T2 (de)
ES (1)	ES2237184T3 (de)
WO (1)	WO2000028077A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
SE9400034D0 (sv) *	1994-01-06	1994-01-06	Glycorex Ab	Laktosaminderivat
US6518036B1 (en) *	1999-03-17	2003-02-11	Nen Life Science Products, Inc.	Method of permanent fluorescent assay
JP2002280842A (ja) *	2001-03-21	2002-09-27	Hitachi Ltd	電力増幅器モジュール
WO2002087530A1 (en) *	2001-04-27	2002-11-07	The General Hospital Corporation	Tyrosinase assay
US6617125B2 (en) *	2001-06-29	2003-09-09	Perkinelmer Life Sciences, Inc.	Compositions for enhanced catalyzed reporter deposition
US20090163375A1 (en)	2003-09-09	2009-06-25	Bowman Christopher N	Use of Photopolymerization for Amplification and Detection of a Molecular Recognition Event
US7354706B2 (en) *	2003-09-09	2008-04-08	The Regents Of The University Of Colorado, A Body Corporate	Use of photopolymerization for amplification and detection of a molecular recognition event
PL209602B1 (pl)	2005-10-20	2011-09-30	Inst Chemii Bioorg Pan	Sposób otrzymywania amidu kwasu 3-(4-hydroksyfenylo)propanowego
ES2624450T3 (es)	2007-09-18	2017-07-14	Dako Denmark A/S	Método rápido y sensible para la detección de dianas biológicas
US20090137405A1 (en) *	2007-11-16	2009-05-28	Christopher Bowman	Detection of nucleic acid biomarkers using polymerization-based amplification
EP2398908B1 (de)	2009-02-19	2013-08-21	Dako Denmark A/S	Verfahren und verbindungen zum nachweis molekularer ziele
CN104714007B (zh) *	2009-10-20	2017-04-12	丹麦达科有限公司	单个靶实体的免疫化学检测
AU2011274369A1 (en)	2010-07-02	2012-12-06	Ventana Medical Systems, Inc.	Hapten conjugates for target detection
SG190892A1 (en)	2010-12-30	2013-07-31	Ventana Med Syst Inc	Enhanced deposition of chromogens utilizing pyrimidine analogs
ES2678211T3 (es)	2012-03-27	2018-08-09	Ventana Medical Systems, Inc.	Conjugados de señalización y procedimientos de uso
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- 1999-11-08 CA CA002351579A patent/CA2351579C/en not_active Expired - Lifetime
- 1999-11-08 EP EP99958831A patent/EP1129214B1/de not_active Expired - Lifetime
- 1999-11-08 ES ES99958831T patent/ES2237184T3/es not_active Expired - Lifetime
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Also Published As

Publication number	Publication date
EP1129214A4 (de)	2002-06-19
AU1611500A (en)	2000-05-29
EP1129214A1 (de)	2001-09-05
CA2351579C (en)	2010-01-19
EP1129214B1 (de)	2005-02-02
US6372937B1 (en)	2002-04-16
WO2000028077A1 (en)	2000-05-18
US6593100B2 (en)	2003-07-15
DE69923580D1 (de)	2005-03-10
CA2351579A1 (en)	2000-05-18
AU768695B2 (en)	2004-01-08
DE69923580T2 (de)	2006-01-05
ATE288497T1 (de)	2005-02-15
US20010039035A1 (en)	2001-11-08

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