ES2501015T3 - Procedimiento de preparación y purificación de fosfatidilserina - Google Patents

Procedimiento de preparación y purificación de fosfatidilserina Download PDF

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Publication number
ES2501015T3
ES2501015T3 ES11171047.1T ES11171047T ES2501015T3 ES 2501015 T3 ES2501015 T3 ES 2501015T3 ES 11171047 T ES11171047 T ES 11171047T ES 2501015 T3 ES2501015 T3 ES 2501015T3
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ES
Spain
Prior art keywords
phosphatidylserine
purification
preparation procedure
pld
serine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES11171047.1T
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English (en)
Inventor
Anna Maria Zanellato
Mara Pittarello
Antonio Gambillara
Susanna Vaccaro
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fidia Farmaceutici SpA
Original Assignee
Fidia Farmaceutici SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fidia Farmaceutici SpA filed Critical Fidia Farmaceutici SpA
Application granted granted Critical
Publication of ES2501015T3 publication Critical patent/ES2501015T3/es
Anticipated expiration legal-status Critical
Active legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J7/00—Phosphatide compositions for foodstuffs, e.g. lecithin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/66—Phosphorus compounds
    • A61K31/683—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols
    • A61K31/685—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols one of the hydroxy compounds having nitrogen atoms, e.g. phosphatidylserine, lecithin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/08—Esters of oxyacids of phosphorus
    • C07F9/09—Esters of phosphoric acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/14—Liposomes; Vesicles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/55—Phosphorus compounds
    • A61K8/553—Phospholipids, e.g. lecithin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/08—Esters of oxyacids of phosphorus
    • C07F9/09—Esters of phosphoric acids
    • C07F9/10—Phosphatides, e.g. lecithin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Birds (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Neurosurgery (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Neurology (AREA)
  • Medicinal Chemistry (AREA)
  • Dermatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Hospice & Palliative Care (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Psychiatry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicinal Preparation (AREA)
  • Cosmetics (AREA)

Abstract

Procedimiento de separación y purificación de fosfatidilserina (PS) de fórmula en la que R1 y R2 representan independientemente un acilo C10-C30, saturado, mono-insaturado o poliinsaturado, X>=OH u OM, en la que M >= metal alcalino o alcalinotérreo, amonio, alquilamonio (incluyendo la sal interna) de serina en forma D, L o racémica, a partir de un compuesto de fórmula en la que R1 y R2 y X tienen los significados especificados anteriormente, R3>=CH2-CH2-NH2 o CH2-CH2-N+(CH3)3 y de una enzima fosfolipasa D (PLD) que queda en un medio en el que la reacción de transfofatidilación ha tenido lugar, estando dicho medio seleccionado de: -un medio hidroalcohólico que contiene un alcohol alifático y en presencia de óxido de metal bivalente; -un medio aprótico que consiste en disolventes acuosos/polares y en presencia de un óxido de metal bivalente; o un sistema de dos fases formado por disolvente acuoso/orgánico y en presencia de un óxido de metal bivalente, implicando dicho procedimiento las siguientes etapas: I) añadir una solución salina de cloruro de sodio al medio de reacción que contiene la PS que se ha producido, con la subsiguiente mezcla y separación de la PS; II) recogida y eliminación del sobrenadante; III) las etapas I y II pueden repetirse modificando la concentración de partida de solución de cloruro de sodio y eliminando el sobrenadante; IV) añadir una solución de EDTA para quelar los iones presentes en la solución y la mezcla subsiguiente; V) añadir una solución de etanol que consiste en etanol en un porcentaje del 50 al 100 % o una mezcla de agua/acetona que consiste en acetona a un porcentaje del 50 al 95 % con mezclado y sedimentación de la PS subsiguientes; VI) la etapa V se puede repetir modificando el porcentaje de etanol; VII) añadir una solución de etanol que consiste en etanol a un porcentaje del 90 al 100 %; VIII) recogida y eliminación del sobrenadante; IX) secado del producto final.

Description

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Claims (1)

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ES11171047.1T 2005-05-30 2006-05-26 Procedimiento de preparación y purificación de fosfatidilserina Active ES2501015T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
ITPD20050164 2005-05-30
IT000164A ITPD20050164A1 (it) 2005-05-30 2005-05-30 Processo per la preparazione e l'isolamento di fosfatidi
US70387005P 2005-08-01 2005-08-01
US703870P 2005-08-01

Publications (1)

Publication Number Publication Date
ES2501015T3 true ES2501015T3 (es) 2014-10-01

Family

ID=39449427

Family Applications (2)

Application Number Title Priority Date Filing Date
ES06753893T Active ES2382748T3 (es) 2005-05-30 2006-05-26 Procedimiento de preparación y aislamiento de fosfátidos
ES11171047.1T Active ES2501015T3 (es) 2005-05-30 2006-05-26 Procedimiento de preparación y purificación de fosfatidilserina

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES06753893T Active ES2382748T3 (es) 2005-05-30 2006-05-26 Procedimiento de preparación y aislamiento de fosfátidos

Country Status (17)

Country Link
US (1) US7759095B2 (es)
EP (2) EP1890706B1 (es)
JP (1) JP5113042B2 (es)
KR (1) KR101298560B1 (es)
CN (1) CN101184494B (es)
AT (1) ATE546145T1 (es)
AU (1) AU2006254376B2 (es)
BR (1) BRPI0611316B8 (es)
CA (1) CA2610575C (es)
ES (2) ES2382748T3 (es)
IL (1) IL187749A (es)
IT (1) ITPD20050164A1 (es)
MX (1) MX2007015064A (es)
NO (1) NO337009B1 (es)
PL (2) PL1890706T3 (es)
PT (2) PT2431020E (es)
WO (1) WO2006128639A2 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITPD20050164A1 (it) 2005-05-30 2006-11-30 Fidia Farmaceutici Processo per la preparazione e l'isolamento di fosfatidi
WO2008148201A1 (en) 2007-06-05 2008-12-11 Bank Of Canada Ink or toner compositions, methods of use, and products derived therefrom
CN102439125B (zh) * 2010-12-03 2014-04-30 上海赢贝生物技术有限公司 一种制备富含多不饱和双键脂肪酰基的磷脂酰丝氨酸的高品质南极磷虾油的方法
CN102485899A (zh) * 2010-12-03 2012-06-06 张永志 一种制备富含多不饱和双键脂肪酰基的磷脂酰丝氨酸的高品质南极磷虾油的方法
ITPD20120021A1 (it) 2012-01-26 2013-07-27 Fidia Farmaceutici "nuove composizioni farmaceutiche contenenti fosfatidilserina e curcumina".
CN104327114A (zh) * 2014-11-06 2015-02-04 江南大学 一种磷脂酰丝氨酸的制备方法
CA3137012A1 (en) * 2019-04-16 2020-10-22 Helixbind, Inc. Methods and devices for ultrasensitive direct detection of microorganisms
WO2021134439A1 (zh) * 2019-12-31 2021-07-08 邦泰生物工程(深圳)有限公司 一种提高磷脂酶d转脂活性的方法以及用其生产磷脂酰丝氨酸的方法

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3869482A (en) * 1972-07-31 1975-03-04 Etapharm Chem Pharm Lab G M B Method of producing highly purified phosphatides
AT315359B (de) * 1972-07-31 1974-05-27 Etapharm Chem Pharm Lab Ges M Verfahren zur Herstellung von hochgereinigten Phosphatiden aus tierischen Organen
JPS63245684A (ja) * 1987-03-31 1988-10-12 Japanese Res & Dev Assoc Bio Reactor Syst Food Ind ホスフアチジン酸誘導体の製造法
ES2063194T3 (es) * 1989-05-26 1995-01-01 Kao Corp Procedimiento de produccion de acido fosfatidico.
IT1260149B (it) 1992-04-17 1996-03-28 Fidia Spa Metodo per la preparazione e purificazione di miscele di fosfolipidi prive di contaminanti da virus non convenzionali
JP3053537B2 (ja) * 1994-11-08 2000-06-19 株式会社ヤクルト本社 脳機能改善剤
US5700668A (en) 1995-12-08 1997-12-23 Italfarmaco Sud S.P.A. Process for the industrial preparation of phosphatidylserine
DE19917249C2 (de) 1999-02-26 2001-09-27 Meyer Lucas Gmbh & Co Verfahren zur Herstellung von Phosphatidylserin-Produkten
IT1311929B1 (it) 1999-04-28 2002-03-20 Chemi Spa Procedimento per la preparazione di fosfatidilserine.
DE19926770A1 (de) 1999-06-11 2000-12-14 Basf Ag Nukleinsäurefragment und Vektor, enthaltend eine Halogenase, sowie ein Verfahren zur Halogenierung chemischer Verbindungen
AU780828B2 (en) * 1999-06-15 2005-04-21 Yissum Research Development Company Of The Hebrew University Of Jerusalem Enzymatic preparation of phospholipids in aqueous media
WO2001088835A1 (en) * 2000-05-15 2001-11-22 Identix Incorporated Fingerprint imaging device
JP4298902B2 (ja) 2000-08-09 2009-07-22 株式会社ヤクルト本社 リン脂質の製造法
IT1319679B1 (it) 2000-12-05 2003-10-23 Chemi Spa Processo di purificazione di fosfatidilserina.
ITPD20010031A1 (it) 2001-02-09 2002-08-09 Fidia Farmaceutici Procedimento per la preparazione di fosfatidi puri e loro impiego in campo cosmetico, farmaceutico ed alimentare.
DE10142014B4 (de) * 2001-08-28 2004-11-11 Degussa Bioactives Deutschland Gmbh & Co. Kg Verfahren zur Herstellung von Phosphatidylserin
DE10149108A1 (de) * 2001-10-05 2003-04-30 Degussa Bioactives Deutschland Verwendung von Phosphatidylserin zur Behandlung des Aufmerksamkeits-Defizit-Syndroms (ADHS)
AU2002348971A1 (en) * 2001-10-12 2003-04-28 Stefana M. Petrescu Ph-sensitive liposomes for targeted drug delivery
DE10217555A1 (de) 2002-04-19 2004-02-19 Degussa Bioactives Deutschland Gmbh Physiologisch verträgliche, Phospholipid-haltige, stabile und harte Matrix
DE10340740A1 (de) * 2003-09-04 2005-03-31 Degussa Food Ingredients Gmbh Physiologisch aktive Zusammensetzung auf Phosphatidylserin-Basis
DE102004002053A1 (de) * 2004-01-15 2005-08-04 Bioghurt Biogarde Gmbh & Co. Kg Verfahren zur Herstellung von Phosphatidylserin und dessen Reinigung durch Extraktion
ITPD20050164A1 (it) 2005-05-30 2006-11-30 Fidia Farmaceutici Processo per la preparazione e l'isolamento di fosfatidi

Also Published As

Publication number Publication date
BRPI0611316B1 (pt) 2020-04-07
CN101184494A (zh) 2008-05-21
PT1890706E (pt) 2012-05-25
WO2006128639A3 (en) 2007-08-09
BRPI0611316A2 (pt) 2010-08-31
PL2431020T3 (pl) 2015-01-30
BRPI0611316B8 (pt) 2021-05-25
NO337009B1 (no) 2015-12-21
KR20080011678A (ko) 2008-02-05
US7759095B2 (en) 2010-07-20
IL187749A (en) 2014-04-30
ITPD20050164A1 (it) 2006-11-30
CN101184494B (zh) 2012-03-21
PL1890706T3 (pl) 2012-07-31
ES2382748T3 (es) 2012-06-13
PT2431020E (pt) 2014-09-16
NO20076158L (no) 2007-11-29
AU2006254376A1 (en) 2006-12-07
JP5113042B2 (ja) 2013-01-09
CA2610575C (en) 2014-09-30
US20080275006A1 (en) 2008-11-06
CA2610575A1 (en) 2006-12-07
EP1890706B1 (en) 2012-02-22
MX2007015064A (es) 2008-02-19
EP1890706A2 (en) 2008-02-27
ATE546145T1 (de) 2012-03-15
EP2431020B1 (en) 2014-07-16
KR101298560B1 (ko) 2013-08-22
IL187749A0 (en) 2008-04-13
WO2006128639A2 (en) 2006-12-07
JP2008545420A (ja) 2008-12-18
AU2006254376B2 (en) 2011-04-21
HK1119584A1 (en) 2009-03-13
EP2431020A1 (en) 2012-03-21

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