ES2525799T3 - Anticuerpos contra troponina I y métodos de uso de los mismos - Google Patents

Anticuerpos contra troponina I y métodos de uso de los mismos Download PDF

Info

Publication number
ES2525799T3
ES2525799T3 ES10704731.8T ES10704731T ES2525799T3 ES 2525799 T3 ES2525799 T3 ES 2525799T3 ES 10704731 T ES10704731 T ES 10704731T ES 2525799 T3 ES2525799 T3 ES 2525799T3
Authority
ES
Spain
Prior art keywords
antibodies
troponin
antibody
methods
constant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES10704731.8T
Other languages
English (en)
Inventor
Susan E. Brophy
Bailin Tu
Dagang Huang
Robert N. Ziemann
Joan D. Tyner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2525799T3 publication Critical patent/ES2525799T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4716—Muscle proteins, e.g. myosin, actin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Vascular Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cardiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Urology & Nephrology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Línea celular de ovario de hámster chino (CHO), referida como TnI 19C7 AM1 hG1 CHO 204, designada por la American Type Culture Collection (ATCC) con el número de depósito PTA-9816.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8
E10704731
10-12-2014
[0048] El término "constructo de anticuerpo", tal como se utiliza en el presente documento, se refiere a un polipéptido que comprende una o más de partes de unión a antígeno de la presente invención unidas a un polipéptido enlazador
o un dominio constante de inmunoglobulina. Los polipéptidos enlazadores comprenden dos o más residuos de
5 aminoácidos unidos por enlaces peptídicos y se utilizan para enlazar una o más partes de unión a antígeno. Dichos polipéptidos enlazadores son bien conocidos en la técnica (véase, por ejemplo, Holliger, P., et al. (1993) Proc. Natl. Acad. Sci. USA 90: 6444-6448; Poljak, RJ, et al. (1994) Structure 2:1121-1123). Un dominio constante de inmunoglobulina se refiere a un dominio constante de cadena pesada o ligera. Las secuencias de aminoácidos del dominio constante de cadena pesada y de cadena ligera de IgG humana se conocen en la técnica, y los ejemplos se
10 presentan en la Tabla 3.
TABLA 3 SEQUENCIA DEL DOMINIO CONSTANTE DE CADENA PESADA Y DEL DOMINIO CONSTANTE DE CADENA LIGERA DE IgG HUMANA
Proteína
Identificador de secuencia Secuencia
12345678901234567890123456789012
Región constante de Ig gamma-1
SEQ ID NO: 50 ASTKGPSVFFLAPSSKSTSGGTAALGCLVKDYFP EPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSV VTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS CDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNA KTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKC KVSNKALPAPIEKTISKAKGQPREPQVYTLPPSR EEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPEN NYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVF SCSVMHEALHNHYTQKSLSLSPGK
Mutante de la región
SEQ ID NO: 51 STKGPSVFPLAPSSKSTSGGTAALGCLVKDYFP
constante de Ig gamma-1
EPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSV VTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS CDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNA KTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKC KVSNKALPAPIEKTISKAKGQPREPQVYTLPPSR EEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPEN NYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVF SCSVMHEALHNHYTQKSLSLSPGK
Región constante de IG kappa
SEQ ID NO: 61 TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPR EAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLS STLTLSKADYEKHKVYACEVTHQGLSSPVTKSFN RGEC
Región constante de IG lambda
SEQ ID NO: 62 QPKAAPSVTLFPPSSEELQANKATLVCLISDFYP GAVTVAWKADSSPVKAGVETTTPSKQSNNKYAAS SYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT ECS
15 [0049] Todavía adicionalmente, un anticuerpo o parte de unión a antígeno del mismo puede ser parte de una molécula de inmunoadhesión más grande, formada mediante asociación covalente o no covalente del anticuerpo o parte de anticuerpo con una o más de otras proteínas o péptidos. Ejemplos de dichas moléculas de inmunoadhesión incluyen el uso de la región del núcleo de estreptavidina para formar una molécula scFv tetramérica (Kipriyanov, SM,
20 et al. (1995) Human antibodies and Hybridomes 6: 93-101) y el uso de un residuo de cisteína, un péptido marcador y una etiqueta de polihistidina C-terminal para formar moléculas scFv bivalentes y biotiniladas (Kipriyanov, SM, et al (1994) Mol. Immunol. 31: 1047-1058). Las partes de anticuerpo, tales como fragmentos Fab y F(ab')2, se pueden preparar a partir de anticuerpos enteros usando técnicas convencionales, tales como la digestión con papaína o pepsina, respectivamente, de anticuerpos enteros. Además, los anticuerpos, partes de anticuerpo y moléculas de
25 inmunoadhesión pueden obtenerse utilizando técnicas estándar de ADN recombinante, tal como se describe en el presente documento.
[0050] Un "anticuerpo aislado", tal como se usa en el presente documento, pretende referirse a un anticuerpo que está sustancialmente libre de otros anticuerpos que tienen especificidades antigénicas diferentes (por ejemplo, un
30 anticuerpo aislado que se une específicamente a troponina I está sustancialmente libre de anticuerpos que se unen específicamente a antígenos distintos de troponina I). Un anticuerpo aislado que se une específicamente a troponina I puede, sin embargo, tener reactividad cruzada con otros antígenos, tales como moléculas de troponina I de otras especies. Además, un anticuerpo aislado puede estar sustancialmente libre de otro material celular y/o productos químicos.
35
10
imagen9
imagen10
imagen11
imagen12
imagen13
imagen14
imagen15
imagen16
imagen17
imagen18
imagen19
imagen20
imagen21
imagen22
imagen23
imagen24
imagen25
imagen26
imagen27
imagen28

Claims (1)

  1. imagen1
    imagen2
ES10704731.8T 2009-02-24 2010-02-23 Anticuerpos contra troponina I y métodos de uso de los mismos Active ES2525799T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/391,937 US8030026B2 (en) 2009-02-24 2009-02-24 Antibodies to troponin I and methods of use thereof
US391937 2009-02-24
PCT/US2010/024979 WO2010099079A1 (en) 2009-02-24 2010-02-23 Antibodies to troponin i and methods of use thereof

Publications (1)

Publication Number Publication Date
ES2525799T3 true ES2525799T3 (es) 2014-12-30

Family

ID=42102721

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10704731.8T Active ES2525799T3 (es) 2009-02-24 2010-02-23 Anticuerpos contra troponina I y métodos de uso de los mismos

Country Status (9)

Country Link
US (4) US8030026B2 (es)
EP (2) EP2401292B1 (es)
JP (3) JP6055593B2 (es)
CN (1) CN102421795B (es)
BR (1) BRPI1007867B8 (es)
CA (1) CA2753541C (es)
ES (1) ES2525799T3 (es)
MX (2) MX359949B (es)
WO (1) WO2010099079A1 (es)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8030026B2 (en) * 2009-02-24 2011-10-04 Abbott Laboratories Antibodies to troponin I and methods of use thereof
UY33826A (es) * 2010-12-22 2012-07-31 Abbott Lab Proteínas de unión con dominios trivariables y sus usos
WO2013084371A1 (ja) * 2011-12-06 2013-06-13 パナソニック株式会社 ヒト心筋由来トロポニンｉに特異的にかつ速やかに結合できる組み換えタンパク質
US8603759B2 (en) 2011-12-06 2013-12-10 Panasonic Corporation Recombinant protein capable of binding specifically and quickly to troponin I derived from human myocardium
US8663933B2 (en) * 2011-12-16 2014-03-04 Panasonic Corporation Recombinant protein capable of binding specifically and quickly to troponin I derived from human myocardium
US8603760B2 (en) * 2011-12-20 2013-12-10 Panasonic Corporation Recombinant protein capable of binding specifically and quickly to troponin I derived from human myocardium
WO2013175678A1 (ja) * 2012-05-24 2013-11-28 パナソニック株式会社 ヒト心筋由来トロポニンｉに特異的にかつ速やかに結合できる変異体タンパク質
US20160116472A1 (en) * 2013-02-04 2016-04-28 The General Hospital Corporation Biomarkers for stroke diagnosis
MX362075B (es) 2013-03-14 2019-01-07 Abbott Lab Ensayo de combinación de antígeno-anticuerpo del virus de la hepatitis c (vhc) y métodos y composiciones para usarlo.
WO2014143343A1 (en) 2013-03-14 2014-09-18 Abbott Laboratories Hcv core lipid binding domain monoclonal antibodies
JP2016512241A (ja) 2013-03-14 2016-04-25 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ 改良された抗体検出のためのｈｃｖｎｓ３組換え抗原およびこの突然変異体
WO2014192202A1 (ja) * 2013-05-29 2014-12-04 パナソニックヘルスケア株式会社 ヒト心筋由来トロポニンｉに特異的にかつ速やかに結合できる組み換えタンパク質
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
WO2017075268A1 (en) 2015-10-27 2017-05-04 Abbott Laboratories Troponin i and soluble urokinase receptor detection for determining the risk of cardiovascular disease
ES2968349T3 (es) 2016-03-31 2024-05-09 Abbott Lab Sistemas basados en árboles de decisión y métodos para estimar el riesgo de síndrome coronario agudo
US20170299612A1 (en) 2016-04-13 2017-10-19 Abbott Laboratories Cardiac troponin i detection during pregnancy for cardiovascular disease identification and risk assessment
WO2017218911A1 (en) 2016-06-17 2017-12-21 Abbott Laboratories BIOMARKERS TO PREDICT NEW ONSET HEART FAILURE WITH PRESERVED EJECTION FRACTION (HFpEF)
EP3279667A1 (en) 2016-08-02 2018-02-07 Abbott Laboratories Cardiac troponin i and copeptin as biomarkers for short-term mortality in acute exacerbation of chronic obstructive pulmonary disease (aecopd)
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
BR112020017941A2 (pt) * 2018-03-14 2021-02-17 F. Hoffmann-La Roche Ag anticorpos, moléculas de ácido nucleico, vetor, composição e método de determinação da troponina t cardíaca humana
WO2019213624A1 (en) * 2018-05-04 2019-11-07 The Regents Of The University Of California Spiked primers for enrichment of pathogen nucleic acids among background of nucleic acids
CN119264211A (zh) 2018-08-27 2025-01-07 瑞泽恩制药公司 拉曼光谱在下游纯化中的应用
CN111018976B (zh) * 2018-10-10 2022-04-01 东莞市朋志生物科技有限公司 一种抗人心肌肌钙蛋白i的重组抗体
CN111018980B (zh) * 2018-10-10 2021-12-03 东莞市朋志生物科技有限公司 一种抗人心肌肌钙蛋白i的抗体及其应用
CN111018974B (zh) 2018-10-10 2022-04-01 东莞市朋志生物科技有限公司 一种抗人心肌肌钙蛋白i的重组抗体
JP7692892B2 (ja) * 2019-08-09 2025-06-16 エフ． ホフマン－ラ ロシュ アーゲー 新規抗トロポニンｔ抗体
JP2025530730A (ja) 2022-08-26 2025-09-17 アボット・ラボラトリーズ Ｉ型及びｉｉ型の心筋梗塞を識別するための心トロポニン及びガレクチン－３の使用
WO2024102383A1 (en) 2022-11-10 2024-05-16 Abbott Laboratories Methods of identifying macrotroponin in biological samples
CN118725099A (zh) * 2023-03-31 2024-10-01 菲鹏生物股份有限公司 cTnI抗体及其用途
CN119504988B (zh) * 2023-08-25 2025-11-18 东莞市朋志生物科技有限公司 一种抗心肌肌钙蛋白i抗体及其应用

Family Cites Families (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4383817A (en) 1982-02-11 1983-05-17 E. I. Du Pont De Nemours And Company Spinneret plate
EP0092918B1 (en) 1982-04-22 1988-10-19 Imperial Chemical Industries Plc Continuous release formulations
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5807715A (en) 1984-08-27 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods and transformed mammalian lymphocyte cells for producing functional antigen-binding protein including chimeric immunoglobulin
US5128326A (en) 1984-12-06 1992-07-07 Biomatrix, Inc. Drug delivery systems based on hyaluronans derivatives thereof and their salts and methods of producing same
US6492107B1 (en) 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
DE229046T1 (de) 1985-03-30 1987-12-17 Marc Genf/Geneve Ballivet Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren.
US5618920A (en) 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
DE3600905A1 (de) 1986-01-15 1987-07-16 Ant Nachrichtentech Verfahren zum dekodieren von binaersignalen sowie viterbi-dekoder und anwendungen
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
JP3101690B2 (ja) 1987-03-18 2000-10-23 エス・ビィ・２・インコーポレイテッド 変性抗体の、または変性抗体に関する改良
US4880078A (en) 1987-06-29 1989-11-14 Honda Giken Kogyo Kabushiki Kaisha Exhaust muffler
US5006309A (en) 1988-04-22 1991-04-09 Abbott Laboratories Immunoassay device with liquid transfer between wells by washing
US5089424A (en) 1988-06-14 1992-02-18 Abbott Laboratories Method and apparatus for heterogeneous chemiluminescence assay
AU4058489A (en) 1988-08-08 1990-03-05 Michael A. Iffiu Collapsible recumbent bicycle with streamlined three-part folding canopy
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
EP0368684B2 (en) 1988-11-11 2004-09-29 Medical Research Council Cloning immunoglobulin variable domain sequences.
GB8826530D0 (en) 1988-11-12 1988-12-14 Ped Capacitors Ltd Electrical capacitors
US5063081A (en) 1988-11-14 1991-11-05 I-Stat Corporation Method of manufacturing a plurality of uniform microfabricated sensing devices having an immobilized ligand receptor
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
GB8901334D0 (en) 1989-01-21 1989-03-15 Oakland Design Products Ltd Improvements relating to broadhead arrows
AU652539B2 (en) 1989-05-16 1994-09-01 Medical Research Council Co-expression of heteromeric receptors
CA2016841C (en) 1989-05-16 1999-09-21 William D. Huse A method for producing polymers having a preselected activity
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
AU6430190A (en) 1989-10-10 1991-05-16 Pitman-Moore, Inc. Sustained release composition for macromolecular proteins
US5139400A (en) 1989-10-11 1992-08-18 Ide Russell D Progressive cavity drive train
CA2071867A1 (en) 1989-11-06 1991-05-07 Edith Mathiowitz Method for producing protein microspheres
GB8928874D0 (en) 1989-12-21 1990-02-28 Celltech Ltd Humanised antibodies
FR2656431B1 (fr) 1989-12-22 1994-06-10 Essilor Int Procede et solution pour decontaminer une lentille souple, en particulier du type hydrophile.
US5006209A (en) * 1990-02-13 1991-04-09 Electrochemical Technology Corp. Electrolytic reduction of alumina
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
DK0585287T3 (da) 1990-07-10 2000-04-17 Cambridge Antibody Tech Fremgangsmåde til fremstilling af specifikke bindingsparelementer
CA2095633C (en) 1990-12-03 2003-02-04 Lisa J. Garrard Enrichment method for variant proteins with altered binding properties
GB9101134D0 (en) 1991-01-18 1991-02-27 R B S Improvements in and relating to accommodation for animals
EP1820858B1 (en) 1991-03-01 2009-08-12 Dyax Corporation Chimeric protein comprising micro-protein having two or more disulfide bonds and embodiments thereof
ATE414768T1 (de) 1991-04-10 2008-12-15 Scripps Research Inst Bibliotheken heterodimerer rezeptoren mittels phagemiden
CA2109528A1 (en) 1991-05-01 1992-11-02 Gregory A. Prince A method for treating infectious respiratory diseases
EP0519596B1 (en) 1991-05-17 2005-02-23 Merck & Co. Inc. A method for reducing the immunogenicity of antibody variable domains
DE4122599C2 (de) 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid zum Screenen von Antikörpern
EP0605522B1 (en) 1991-09-23 1999-06-23 Medical Research Council Methods for the production of humanized antibodies
US5565332A (en) 1991-09-23 1996-10-15 Medical Research Council Production of chimeric antibodies - a combinatorial approach
EP0571613B1 (en) 1991-12-13 2003-09-17 Xoma Corporation Methods and materials for preparation of modified antibody variable domains and therapeutic uses thereof
GB9201755D0 (en) 1992-01-28 1992-03-11 British Bio Technology Compounds
US5714350A (en) 1992-03-09 1998-02-03 Protein Design Labs, Inc. Increasing antibody affinity by altering glycosylation in the immunoglobulin variable region
US5912015A (en) 1992-03-12 1999-06-15 Alkermes Controlled Therapeutics, Inc. Modulated release from biocompatible polymers
US5912120A (en) 1992-04-09 1999-06-15 The United States Of America As Represented By The Department Of Health And Human Services, Cloning, expression and diagnosis of human cytochrome P450 2C19: the principal determinant of s-mephenytoin metabolism
ES2126650T3 (es) 1992-07-10 1999-04-01 Horst Warneke Linea de produccion para la fabricacion de artesones de acero para la construccion de techos y/o paredes a partir de planchas de chapa.
US5639641A (en) 1992-09-09 1997-06-17 Immunogen Inc. Resurfacing of rodent antibodies
US5934272A (en) 1993-01-29 1999-08-10 Aradigm Corporation Device and method of creating aerosolized mist of respiratory drug
US5565352A (en) 1993-11-24 1996-10-15 Arch Development Corporation Deubiquitinating enzyme: compositions and methods
ZA955550B (en) 1994-07-08 1996-02-16 Gillette Co Aqueous correction fluids
GB9425232D0 (en) 1994-12-14 1995-02-08 Secr Defence Method of authenticating watermarked paper
ATE252894T1 (de) 1995-01-05 2003-11-15 Univ Michigan Oberflächen-modifizierte nanopartikel und verfahren für ihre herstellung und verwendung
US5705330A (en) 1995-04-14 1998-01-06 Abbott Laboratories Chemiluminescent immunoassay for antibody detection
US6019968A (en) 1995-04-14 2000-02-01 Inhale Therapeutic Systems, Inc. Dispersible antibody compositions and methods for their preparation and use
WO1997007788A2 (en) 1995-08-31 1997-03-06 Alkermes Controlled Therapeutics, Inc. Composition for sustained release of an agent
MX336813B (es) 1996-02-09 2016-02-02 Abbvie Biotechnology Ltd Anticuerpos humanos que ligan el tnfa humano.
ATE508733T1 (de) 1996-03-04 2011-05-15 Penn State Res Found Materialien und verfahren zur steigerung der zellulären internalisierung
US5714352A (en) 1996-03-20 1998-02-03 Xenotech Incorporated Directed switch-mediated DNA recombination
US5985309A (en) 1996-05-24 1999-11-16 Massachusetts Institute Of Technology Preparation of particles for inhalation
US5855913A (en) 1997-01-16 1999-01-05 Massachusetts Instite Of Technology Particles incorporating surfactants for pulmonary drug delivery
US5874064A (en) 1996-05-24 1999-02-23 Massachusetts Institute Of Technology Aerodynamically light particles for pulmonary drug delivery
US6699658B1 (en) 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US7285418B2 (en) 1996-06-25 2007-10-23 Hytest Ltd. Method and kit for the diagnosis of troponin I
FR2754461B1 (fr) 1996-10-16 1999-02-12 Husson Olivier Fixation demontable manuellement pour patin en ligne avec une jambiere de maintien qui commande un systeme de freinage
CA2277801C (en) 1997-01-16 2002-10-15 Massachusetts Institute Of Technology Preparation of particles for inhalation
US6870033B1 (en) * 1997-02-21 2005-03-22 Genentech, Inc. Antibody fragment-polymer conjugates and humanized anti-IL-8 monoclonal antibodies
JPH10324699A (ja) * 1997-03-21 1998-12-08 Sankyo Co Ltd 抗ヒトＦａｓヒト化抗体
IL123756A0 (en) * 1997-03-21 1998-10-30 Sankyo Co Humanized anti-human FAS antibody
US5989463A (en) 1997-09-24 1999-11-23 Alkermes Controlled Therapeutics, Inc. Methods for fabricating polymer-based controlled release devices
SE512663C2 (sv) 1997-10-23 2000-04-17 Biogram Ab Inkapslingsförfarande för aktiv substans i en bionedbrytbar polymer
US6194222B1 (en) * 1998-01-05 2001-02-27 Biosite Diagnostics, Inc. Methods for monitoring the status of assays and immunoassays
DK1071700T3 (da) 1998-04-20 2010-06-07 Glycart Biotechnology Ag Glykosylerings-modifikation af antistoffer til forbedring af antistofafhængig cellulær cytotoksicitet
FR2779526A1 (fr) 1998-06-09 1999-12-10 Pasteur Sanofi Diagnostics Nouveau procede de dosage de la troponine i cardiaque
WO1999066903A2 (en) 1998-06-24 1999-12-29 Advanced Inhalation Research, Inc. Large porous particles emitted from an inhaler
HUP0200575A3 (en) * 1999-03-25 2004-11-29 Abbott Gmbh & Co Kg Human antibodies that bind human il-12 and methods for producing
CA2704600C (en) 1999-04-09 2016-10-25 Kyowa Kirin Co., Ltd. A method for producing antibodies with increased adcc activity
CN1247615C (zh) * 2000-03-21 2006-03-29 上海润东生物科技有限公司 人体心肌肌钙蛋白ⅰ的纯化和其单克隆抗体的制备方法
AU5943201A (en) 2000-05-03 2001-11-12 Amgen Inc Modified peptides as therapeutic agents
ES2252261T3 (es) 2000-06-28 2006-05-16 Glycofi, Inc. Metodos para producir glicoproteinas modificadas.
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
KR20030074693A (ko) 2000-12-28 2003-09-19 알투스 바이올로직스 인코포레이티드 전항체 및 이의 단편의 결정과 이의 제조 및 사용 방법
CA2451998A1 (en) 2001-08-17 2003-02-27 Eli Lilly And Company Anti-a.beta. antibodies
DK2093286T3 (da) 2001-10-01 2013-05-13 Dyax Corp Flerkædede, eukaryote displayvektorer og anvendelser deraf
CA2463879C (en) 2001-10-25 2012-12-04 Genentech, Inc. Glycoprotein compositions
US7419821B2 (en) 2002-03-05 2008-09-02 I-Stat Corporation Apparatus and methods for analyte measurement and immunoassay
WO2003086458A1 (en) * 2002-04-12 2003-10-23 Medimmune, Inc. Recombinant anti-interleukin-9 antibodies
US20040018577A1 (en) 2002-07-29 2004-01-29 Emerson Campbell John Lewis Multiple hybrid immunoassay
CN1802388B (zh) 2003-05-09 2011-01-05 杜克大学 Cd20特异抗体及使用它们的方法
US7682833B2 (en) 2003-09-10 2010-03-23 Abbott Point Of Care Inc. Immunoassay device with improved sample closure
US7723099B2 (en) 2003-09-10 2010-05-25 Abbott Point Of Care Inc. Immunoassay device with immuno-reference electrode
AU2005233387B2 (en) 2004-04-15 2011-05-26 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
WO2006004910A2 (en) 2004-06-28 2006-01-12 Transtarget Inc. Improved bispecific antibodies
CN1644685A (zh) * 2004-12-23 2005-07-27 复旦大学 抗人心肌肌钙蛋白i单克隆抗体及其应用
CN1982337A (zh) * 2005-12-14 2007-06-20 中国医学科学院放射医学研究所 抗人心肌肌钙蛋白i合成多肽抗体及其制备方法与应用
EP2014302A1 (en) 2007-07-12 2009-01-14 Institut Curie An antibody specific for the Tn antigen for the treatment of cancer
JP5470817B2 (ja) 2008-03-10 2014-04-16 日産自動車株式会社 電池用電極およびこれを用いた電池、並びにその製造方法
US8030026B2 (en) 2009-02-24 2011-10-04 Abbott Laboratories Antibodies to troponin I and methods of use thereof
JP6136279B2 (ja) 2013-01-15 2017-05-31 株式会社ジェイテクト 転がり軸受装置
TWI503850B (zh) 2013-03-22 2015-10-11 Polytronics Technology Corp 過電流保護元件
TWI510996B (zh) 2013-10-03 2015-12-01 Acer Inc 控制觸控面板的方法以及使用該方法的可攜式電腦
US9816280B1 (en) 2016-11-02 2017-11-14 Matthew Reitnauer Portable floor

Also Published As

Publication number Publication date
BRPI1007867B1 (pt) 2021-01-26
BRPI1007867A2 (pt) 2018-03-06
US8030026B2 (en) 2011-10-04
JP6055593B2 (ja) 2016-12-27
CN102421795A (zh) 2012-04-18
BRPI1007867B8 (pt) 2021-05-25
JP2018038392A (ja) 2018-03-15
CN102421795B (zh) 2015-11-25
JP2012518418A (ja) 2012-08-16
US20120076803A1 (en) 2012-03-29
USRE45763E1 (en) 2015-10-20
MX2011008938A (es) 2012-04-02
JP6199935B2 (ja) 2017-09-20
EP2401292B1 (en) 2014-10-29
US20150024457A1 (en) 2015-01-22
CA2753541A1 (en) 2010-09-02
JP2016039804A (ja) 2016-03-24
EP2853540A1 (en) 2015-04-01
US20100216720A1 (en) 2010-08-26
WO2010099079A1 (en) 2010-09-02
MX359949B (es) 2018-10-17
EP2401292A1 (en) 2012-01-04
CA2753541C (en) 2018-07-17

Similar Documents

Publication Publication Date Title
ES2525799T3 (es) Anticuerpos contra troponina I y métodos de uso de los mismos
Trier et al. Production and characterization of peptide antibodies
ES2608835T3 (es) Proteínas de fusión Fc que comprenden nuevos enlazadores o disposiciones
CN105408357A (zh) 新型抗体
US20210317233A1 (en) Super Versatile Method for Imparting New Binding Specificity to Antibody
CN103298836A (zh) 用于获得抗疏水性肽抗体的抗原的制备方法
CN118580323B (zh) Rsv融合前f蛋白的突变体及其制备和应用
EP3943940A1 (en) Target detection using a monovalent antibody
US20180236065A1 (en) Coiled-coil connector
SA520420339B1 (ar) أجسام مضادة مُطعمة ببتيد أذيني مدر للصوديوم
JPWO2019026920A1 (ja) 環状ペプチドをタンパク質構造に提示させる超汎用法
Mard-Soltani et al. The effect of differentially designed fusion proteins to elicit efficient anti-human thyroid stimulating hormone immune responses
JP2015512247A5 (es)
US20240002475A1 (en) Compositions and methods for rapid production of versatile single domain antibody repertoires
CN118496324A (zh) RSV pre-F突变体及其生产方法和用途
AU707030B2 (en) High level expression and facile purification of proteins, peptides and conjugates for immunization, purification and detection applications
Aston et al. Antigenic structure of bovine growth hormone: location of a growth enhancing region
AR125980A1 (es) Anticuerpos específicamente unidos a la proteína asm
US20250115638A1 (en) Peptide antigens for formylmethionine antibody production
Manea et al. Design, structural, and immuno‐analytical properties of antigenic bioconjugates comprising a β‐amyloid‐plaque specific epitope
KR102140557B1 (ko) 단백질-단백질 결합체를 형성 매개 펩타이드 및 이를 이용한 단백질-단백질 결합체 형성 방법
JP2022181707A (ja) 非変性チャネルロドプシン検出キット
Hisae et al. Repeated helical epitopes of defined amino acid sequence in human type III collagen identified by monoclonal antibodies
Parkkinen et al. Structural Basis for Trivalent Cross‐Linking of a Patient‐Derived IgE Antibody by the Major Peanut Allergen Ara h 2.0201
Kleine et al. Type-specific immunodetection of human heart fatty acid-binding protein with polyclonal anti-peptide antibodies