ES2539254T3 - Método de secuenciación de ADN mediante polimerización - Google Patents

Método de secuenciación de ADN mediante polimerización Download PDF

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Publication number
ES2539254T3
ES2539254T3 ES11722796.7T ES11722796T ES2539254T3 ES 2539254 T3 ES2539254 T3 ES 2539254T3 ES 11722796 T ES11722796 T ES 11722796T ES 2539254 T3 ES2539254 T3 ES 2539254T3
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Prior art keywords
nucleic acid
stranded nucleic
acid molecule
double stranded
double
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ES11722796.7T
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Inventor
David Bensimon
Vincent Croquette
Jean-François Allemand
Maria Manosas
Fang-Yuan Ding
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Centre National de la Recherche Scientifique CNRS
Universite Pierre et Marie Curie
Ecole Normale Superieure de Paris
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Centre National de la Recherche Scientifique CNRS
Universite Pierre et Marie Curie
Ecole Normale Superieure de Paris
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/319—Exonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método para la determinación de una secuencia de ácido nucleico, comprendiendo dicho método las etapas de: a) desnaturalizar una molécula de ácido nucleico bicatenario correspondiente a dicha secuencia de ácido nucleico, en el que al menos una de las bases de una de las hebras del ácido nucleico bicatenario está unida directa o indirectamente a un soporte, y en el que al menos una de las bases de la otra hebra del ácido nucleico bicatenario está unida a un soporte móvil; b) hibridar una molécula de ácido nucleico monocatenario, "el cebador", con dicha molécula de ácido nucleico bicatenario desnaturalizada; c) aplicar una tensión al cebador hibridado/molécula de ácido nucleico bicatenario obtenido en b); d) incubar el cebador hibridado/molécula de ácido nucleico bicatenario obtenido en la etapa b) con una polimerasa en condiciones que conducirán a por lo menos una pausa en la replicación; y e) determinar la posición de dicha pausa en la replicación con respecto a un extremo del ácido nucleico bicatenario, comprendiendo dicha determinación las etapas de: - medir la distancia (z) entre los dos extremos de la molécula de ácido nucleico bicatenario que están unidos al soporte; - medir la distancia (zhigh) entre los dos extremos de la molécula de ácido nucleico bicatenario que están unidos al soporte, cuando dicha molécula de ácido nucleico bicatenario está desnaturalizada; y - comparar z y zhigh, y - determinar la posición de la pausa, en el que dicha molécula de ácido nucleico bicatenario es una horquilla.

Description

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Claims (1)

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ES11722796.7T 2010-05-27 2011-05-26 Método de secuenciación de ADN mediante polimerización Active ES2539254T3 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP10305563 2010-05-27
EP10305563A EP2390350A1 (en) 2010-05-27 2010-05-27 Method of DNA sequencing by polymerisation
US37762110P 2010-08-27 2010-08-27
US377621P 2010-08-27
PCT/EP2011/058664 WO2011147929A1 (en) 2010-05-27 2011-05-26 Method of dna sequencing by polymerisation

Publications (1)

Publication Number Publication Date
ES2539254T3 true ES2539254T3 (es) 2015-06-29

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ES11722796.7T Active ES2539254T3 (es) 2010-05-27 2011-05-26 Método de secuenciación de ADN mediante polimerización

Country Status (11)

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US (2) US9493829B2 (es)
EP (2) EP2390350A1 (es)
JP (2) JP2013528380A (es)
KR (1) KR101848377B1 (es)
CN (1) CN103097551B (es)
AU (1) AU2011257227B2 (es)
CA (1) CA2800637C (es)
DK (1) DK2576822T3 (es)
ES (1) ES2539254T3 (es)
IL (1) IL223256A (es)
WO (1) WO2011147929A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
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EP2390350A1 (en) * 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by polymerisation
EP2390351A1 (en) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by hybridisation
US9862987B2 (en) * 2013-01-16 2018-01-09 The Regents Of The University Of California Label free molecular detection methods, systems and devices
WO2014113598A2 (en) 2013-01-16 2014-07-24 The Regents Of The University Of California Microfluidic devices to extract, concentrate and isolate molecules
SG11201505618XA (en) * 2013-01-22 2015-08-28 Centre Nat Rech Scient Process for detection of dna modifications and protein binding by single molecule manipulation
EP3292217B1 (en) 2015-05-07 2019-07-10 Paris Sciences et Lettres - Quartier Latin Formation of hairpins in situ using force-induced strand invasion
EP3090803B1 (en) 2015-05-07 2019-08-07 Paris Sciences et Lettres - Quartier Latin Improved device for the analysis of nucleic acid molecules
US10344326B2 (en) 2016-05-25 2019-07-09 International Business Machines Corporation Magnetic flux density based DNA sequencing
AU2018272302A1 (en) * 2017-05-26 2019-12-19 Nuclera Nucleics Ltd Use of terminal transferase enzyme in nucleic acid synthesis
EP3625356B1 (en) 2017-08-08 2021-05-19 Depixus In vitro isolation and enrichment of nucleic acids using site-specific nucleases
CN113330122A (zh) 2018-11-16 2021-08-31 德皮克斯公司 使用位点特异性核酸酶优化核酸的体外分离
IL283866B2 (en) 2018-12-12 2023-02-01 Depixus Nucleic acid enrichment method using site-specific nuclei, which appear by capture
EP4160199B1 (en) 2021-10-04 2025-02-12 Depixus Apparatus for biomolecule analysis with a well and a cavity below the well
EP4481057A1 (en) 2023-06-20 2024-12-25 Depixus Sample preparation methods using modified bases for rapid identification and sequencing

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IL73577A (en) 1983-12-12 1989-10-31 Miles Lab Method and reagent system for detecting dna or rna sequences in a test medium containing single stranded dna or rna using antibodies to intercalation complexes with double stranded nucleic acid
CA1223222A (en) 1984-02-22 1987-06-23 Nanibhushan Dattagupta Immobilized nucleic acid-containing probes
US4849077A (en) 1984-08-06 1989-07-18 Akademie Der Wissenschaften Der Ddr Process for solid phase-sequencing of nucleic acid fragments
JPH0830704B2 (ja) 1991-03-21 1996-03-27 イーストマン コダック カンパニー 核酸増幅のための要素および方法ならびに付着プローブを使用する検出方法
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Also Published As

Publication number Publication date
IL223256A (en) 2017-10-31
DK2576822T3 (en) 2015-06-29
JP2016214250A (ja) 2016-12-22
US9493829B2 (en) 2016-11-15
HK1183912A1 (en) 2014-01-10
US20170073749A1 (en) 2017-03-16
JP6325028B2 (ja) 2018-05-16
CA2800637C (en) 2018-08-28
WO2011147929A1 (en) 2011-12-01
AU2011257227A1 (en) 2013-01-24
KR101848377B1 (ko) 2018-04-13
IL223256A0 (en) 2013-02-03
EP2390350A1 (en) 2011-11-30
CN103097551B (zh) 2016-01-20
EP2576822B1 (en) 2015-03-18
AU2011257227B2 (en) 2015-07-09
JP2013528380A (ja) 2013-07-11
US20130171636A1 (en) 2013-07-04
CN103097551A (zh) 2013-05-08
KR20130123302A (ko) 2013-11-12
CA2800637A1 (en) 2011-12-01
US9738928B2 (en) 2017-08-22
EP2576822A1 (en) 2013-04-10

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