ES2564455T3 - Método de autenticación de productos lácteos - Google Patents

Método de autenticación de productos lácteos Download PDF

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Publication number
ES2564455T3
ES2564455T3 ES10779483.6T ES10779483T ES2564455T3 ES 2564455 T3 ES2564455 T3 ES 2564455T3 ES 10779483 T ES10779483 T ES 10779483T ES 2564455 T3 ES2564455 T3 ES 2564455T3
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Spain
Prior art keywords
region
nucleic acid
absence
bacterial strain
pair
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ES10779483.6T
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English (en)
Inventor
Hélène BERTHOUD
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Agroscope Liebefeld Posieux ALP
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Agroscope Liebefeld Posieux ALP
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Publication of ES2564455T3 publication Critical patent/ES2564455T3/es
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0323—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin using only lactic acid bacteria, e.g. Pediococcus and Leuconostoc species; Bifidobacteria; Microbial starters in general
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/137—Delbrueckii
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/41—Pediococcus
    • A23V2400/413—Acidilactici

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Dairy Products (AREA)

Abstract

Método para identificar la presencia o la ausencia de una cepa bacteriana acidoláctica que comprende un elemento IS localizado de forma exclusiva en un producto lácteo, comprendiendo el método: (a) obtener una muestra de ácido nucleico de un producto lácteo, (b) proporcionar un par de cebadores específicos para una región de dicho elemento IS localizado de forma exclusiva y una región de una secuencia de ácido nucleico adyacente a dicho elemento IS localizado de forma univoca, (c) realizar una reacción de amplificación por PCR con dicho par de cebadores de la epata (b) en condiciones adecuadas para producir un producto de amplificación cuando dicho elemento IS localizado de forma exclusiva está presente en dicha muestra de ácido nucleico, y (d) identificar la presencia o la ausencia de una cepa bacteriana acidoláctica detectando la presencia o la ausencia de dicho producto de amplificación, en donde dicha cepa bacteriana acidoláctica es la cepa de Pediococcus, Pediococcus acidilactici, representada por el aislado depositado el 25 de septiembre de 2009 en la Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ) con el número de acceso DSM 22981, y en donde el par de cebadores específico para una región de dicho elemento IS localizado de forma exclusiva y una región de una secuencia de ácido nucleico adyacente a dicho elemento IS localizado de forma exclusiva comprende un primer cebador de la SEC ID N.º 1 y un segundo cebador de la SEC ID N.º 2.

Description

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Claims (1)

  1. imagen1
ES10779483.6T 2009-10-01 2010-10-01 Método de autenticación de productos lácteos Active ES2564455T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09172008 2009-10-01
EP09172008 2009-10-01
PCT/EP2010/064673 WO2011039359A2 (en) 2009-10-01 2010-10-01 Authentication method of dairy products

Publications (1)

Publication Number Publication Date
ES2564455T3 true ES2564455T3 (es) 2016-03-22

Family

ID=41257516

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10779483.6T Active ES2564455T3 (es) 2009-10-01 2010-10-01 Método de autenticación de productos lácteos

Country Status (8)

Country Link
US (1) US8883422B2 (es)
EP (1) EP2483423B1 (es)
CN (1) CN102884202B (es)
DK (1) DK2483423T3 (es)
ES (1) ES2564455T3 (es)
PL (1) PL2483423T3 (es)
RU (1) RU2567658C2 (es)
WO (1) WO2011039359A2 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2121721A4 (en) * 2006-09-07 2010-10-06 Boehringer Ingelheim Vetmed GENOTYPING ON PCR BASE
EP3543362B1 (en) * 2013-10-07 2021-09-08 Mitsui Chemicals, Inc. Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
CN103614469B (zh) * 2013-11-20 2016-03-23 南通市产品质量监督检验所 一种原料乳及牛奶中植物源性蛋白成分鉴别方法
CN108893549A (zh) * 2018-08-16 2018-11-27 山东探克生物科技股份有限公司 一种发酵乳制品中嗜热链球菌实时荧光定量pcr引物对及其应用
CN114277160A (zh) * 2020-09-27 2022-04-05 上海市质量监督检验技术研究院 用于检测杀菌型乳制品中乳酸乳球菌的引物对和探针及其方法和应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19818176A1 (de) 1998-04-23 1999-10-28 Basf Ag Verfahren zur Markierung von Flüssigkeiten mit mindestens zwei Markierstoffen und Verfahren zu deren Detektion
US4764290A (en) 1987-02-02 1988-08-16 National Identification Laboratories, Inc. Identification marking of oils
GB8802237D0 (en) 1988-02-02 1988-03-02 Shell Int Research Detection of chemicals by immunoassay
US5447844A (en) * 1991-03-14 1995-09-05 The United States Of America As Represented By The Secretary Of Agriculture Diagnostic assay for bacteria based on fragment amplification using insertion sequence location
CA2206486A1 (en) 1994-12-08 1996-06-13 Peter Alestrom Chemical labelling of objects
US20040029295A1 (en) 2002-02-14 2004-02-12 Brogger Brian J. Non-toxic biodegradable microtaggants
GB0225129D0 (en) * 2002-10-29 2002-12-11 Syngenta Participations Ag Improvements in or relating to organic compounds
BRPI0509801A (pt) * 2004-04-15 2007-09-18 Hansens Lab método para reduzir o teor de organismo patogênicos presentes em materiais alimentìcios
US20060035288A1 (en) 2004-07-30 2006-02-16 Green Lawrence R Methods for detection of counterfeit liquids and foods
US7972859B2 (en) 2006-06-27 2011-07-05 Authentix, Inc. Authentication of ingestible products using saccharides as markers
ES2322827B1 (es) * 2006-07-03 2010-04-23 Consejo Superior Investig. Cientificas Procedimiento para la deteccion e identificacion simultanea y especifica de bacterias lacticas y bifidobacterias en leches fermentadas y en cultivos iniciadores para leches fermentadas.
DE102006038968A1 (de) 2006-08-21 2008-02-28 Siemens Ag Verfahren zur Überwachung des Zustands eines Motorenöls in einem Verbrennungsmotor
CN101050437A (zh) * 2007-03-21 2007-10-10 华东理工大学 一种乳酸片球菌菌株和乳酸片球菌素的生产方法
GB0718934D0 (en) 2007-09-28 2007-11-07 Johnson Matthey Plc Tagging method

Also Published As

Publication number Publication date
EP2483423B1 (en) 2015-12-16
RU2567658C2 (ru) 2015-11-10
RU2012114730A (ru) 2013-11-10
DK2483423T3 (en) 2016-03-07
US20120329048A1 (en) 2012-12-27
CN102884202A (zh) 2013-01-16
US8883422B2 (en) 2014-11-11
WO2011039359A3 (en) 2011-09-29
PL2483423T3 (pl) 2017-07-31
WO2011039359A2 (en) 2011-04-07
EP2483423A2 (en) 2012-08-08
CN102884202B (zh) 2014-12-31

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