ES2565180T3 - Proceso para la preparación de ácido polisiálico de gran pureza - Google Patents
Proceso para la preparación de ácido polisiálico de gran pureza Download PDFInfo
- Publication number
- ES2565180T3 ES2565180T3 ES07849680.9T ES07849680T ES2565180T3 ES 2565180 T3 ES2565180 T3 ES 2565180T3 ES 07849680 T ES07849680 T ES 07849680T ES 2565180 T3 ES2565180 T3 ES 2565180T3
- Authority
- ES
- Spain
- Prior art keywords
- amount
- acid
- polysalic
- polysalic acid
- coli
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002253 acid Substances 0.000 title abstract 9
- 238000000034 method Methods 0.000 title abstract 3
- 241000894006 Bacteria Species 0.000 abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 2
- 235000015097 nutrients Nutrition 0.000 abstract 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 abstract 1
- 238000006640 acetylation reaction Methods 0.000 abstract 1
- 150000007513 acids Chemical class 0.000 abstract 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 1
- 229940041514 candida albicans extract Drugs 0.000 abstract 1
- 229910000365 copper sulfate Inorganic materials 0.000 abstract 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 abstract 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 abstract 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019797 dipotassium phosphate Nutrition 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 abstract 1
- 235000019341 magnesium sulphate Nutrition 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- 239000001301 oxygen Substances 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000000600 sorbitol Substances 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 239000012138 yeast extract Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Un proceso para la producción de ácido polisiálico con una pureza superior al 95 % y un peso molecular de 70.000 Da a 100.000 Da, en que el proceso comprende: (a) la obtención de un medio nutriente que comprende sorbitol en una cantidad de 8 g/l a 12 g/l, extracto de levadura en una cantidad de 0,4 g/l a 0,6 g/l, casaminoácidos en una cantidad de 8 g/l, hidrogenofosfato de potasio en una cantidad de 8 g/l a 12 g/l, sulfato de cobre en una cantidad de 0,001 g/l a 0,003 g/l y sulfato de magnesio en una cantidad de 0,4 g/l a 0,6 g/l; (b) la inoculación de bacterias productoras de ácido polisiálico pertenecientes al serogrupo O7:K1 de E. coli, que no muestran O-acetilación del ácido polisiálico en dicho medio nutriente, opcionalmente donde la bacteria productora de ácido polisiálico es E. coli LP 1674; (c) la incubación del medio inoculado a una temperatura de 30 °C a 40 °C, a un pH de 6-8, un porcentaje de oxígeno disuelto del 35 % al 45 % y una agitación de 100 a 150 rpm; (d) la recogida del ácido polisiálico producido en la etapa (c); (e) la purificación del ácido polisiálico obtenido en la etapa (d); y (f) opcionalmente la concentración del ácido polisiálico obtenido en la etapa (e).
Description
Claims (1)
-
imagen1 imagen2
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN1110MU2006 | 2006-07-13 | ||
| INMU11102006 | 2006-07-13 | ||
| INMU20832006 | 2006-12-20 | ||
| IN2083MU2006 | 2006-12-20 | ||
| PCT/IN2007/000291 WO2008035373A2 (en) | 2006-07-13 | 2007-07-13 | Highly pure polysialic acid and process for preperation thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2565180T3 true ES2565180T3 (es) | 2016-04-01 |
Family
ID=39200974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07849680.9T Active ES2565180T3 (es) | 2006-07-13 | 2007-07-13 | Proceso para la preparación de ácido polisiálico de gran pureza |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US8097437B2 (es) |
| EP (1) | EP2049692B1 (es) |
| CY (1) | CY1117328T1 (es) |
| DK (1) | DK2049692T3 (es) |
| ES (1) | ES2565180T3 (es) |
| HU (1) | HUE027178T2 (es) |
| PL (1) | PL2049692T3 (es) |
| PT (1) | PT2049692E (es) |
| SI (1) | SI2049692T1 (es) |
| WO (1) | WO2008035373A2 (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2670618C (en) | 2006-12-15 | 2016-10-04 | Baxter International Inc. | Factor viia- (poly) sialic acid conjugate having prolonged in vivo half-life |
| JP2010520320A (ja) | 2007-02-28 | 2010-06-10 | リポクセン テクノロジーズ リミテッド | ポリシアル酸におけるエンドトキシンの低減 |
| US8642737B2 (en) | 2010-07-26 | 2014-02-04 | Baxter International Inc. | Nucleophilic catalysts for oxime linkage |
| KR101912335B1 (ko) | 2009-07-27 | 2018-10-26 | 리폭센 테크놀로지즈 리미티드 | 비혈액 응고 단백질의 글리코폴리시알화 |
| US8809501B2 (en) | 2009-07-27 | 2014-08-19 | Baxter International Inc. | Nucleophilic catalysts for oxime linkage |
| CN102497884A (zh) | 2009-07-27 | 2012-06-13 | 巴克斯特国际公司 | 凝血蛋白缀合物 |
| CN106110311A (zh) | 2009-07-27 | 2016-11-16 | 百深公司 | 凝血蛋白缀合物 |
| HUE049352T2 (hu) | 2010-12-22 | 2020-09-28 | Baxalta GmbH | Anyagok és módszerek egy vízoldható zsírsavszármazéknak egy fehérjéhez való konjugálására |
| CN104450978B (zh) * | 2014-12-24 | 2017-04-12 | 江南大学 | 一种提高大肠杆菌聚唾液酸产量的专家控制系统 |
| EP3283102A4 (en) | 2015-04-16 | 2018-10-03 | Inventprise, LLC. | Bordetella pertussis immunogenic vaccine compositions |
| CN108588152B (zh) * | 2018-05-14 | 2021-06-04 | 中国科学院合肥物质科学研究院 | 聚唾液酸发酵培养基、聚唾液酸的生产方法和聚唾液酸制品 |
| CN109182423B (zh) * | 2018-09-26 | 2020-07-07 | 武汉中科光谷绿色生物技术有限公司 | 促进大肠杆菌发酵生产聚唾液酸的方法 |
| CN112553120B (zh) * | 2020-12-25 | 2021-09-14 | 华熙生物科技股份有限公司 | 一株大肠埃希氏菌及其在产聚唾液酸中的应用 |
| CN114621892B (zh) * | 2021-12-17 | 2024-04-05 | 嘉必优生物技术（武汉）股份有限公司 | 高产聚唾液酸的大肠杆菌及其应用 |
| CN114878705B (zh) * | 2022-04-20 | 2023-10-27 | 中轻食品检验认证有限公司 | 一种测定燕窝制品中唾液酸稳定碳同位素比值的方法 |
| CN115028669A (zh) * | 2022-07-15 | 2022-09-09 | 山东润德生物科技有限公司 | 一种采用发酵法制备唾液酸的工艺及其应用 |
| CN119912505A (zh) * | 2025-04-02 | 2025-05-02 | 浙江熙正霖生物科技有限公司 | 一种从发酵液中提纯唾液酸的方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2620795B2 (ja) * | 1987-11-30 | 1997-06-18 | 丸金醤油株式会社 | コロミン酸の製造法 |
-
2007
- 2007-07-13 WO PCT/IN2007/000291 patent/WO2008035373A2/en not_active Ceased
- 2007-07-13 US US12/373,571 patent/US8097437B2/en active Active
- 2007-07-13 DK DK07849680.9T patent/DK2049692T3/en active
- 2007-07-13 EP EP07849680.9A patent/EP2049692B1/en active Active
- 2007-07-13 SI SI200731743T patent/SI2049692T1/sl unknown
- 2007-07-13 ES ES07849680.9T patent/ES2565180T3/es active Active
- 2007-07-13 HU HUE07849680A patent/HUE027178T2/en unknown
- 2007-07-13 PT PT78496809T patent/PT2049692E/pt unknown
- 2007-07-13 PL PL07849680T patent/PL2049692T3/pl unknown
-
2016
- 2016-03-01 CY CY20161100180T patent/CY1117328T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP2049692B1 (en) | 2016-01-06 |
| EP2049692A4 (en) | 2013-01-02 |
| PL2049692T3 (pl) | 2016-08-31 |
| HUE027178T2 (en) | 2016-10-28 |
| SI2049692T1 (sl) | 2016-04-29 |
| DK2049692T3 (en) | 2016-04-04 |
| WO2008035373A3 (en) | 2009-02-26 |
| WO2008035373A4 (en) | 2009-04-16 |
| CY1117328T1 (el) | 2017-04-26 |
| PT2049692E (pt) | 2016-02-22 |
| US8097437B2 (en) | 2012-01-17 |
| US20100086974A1 (en) | 2010-04-08 |
| WO2008035373A2 (en) | 2008-03-27 |
| EP2049692A2 (en) | 2009-04-22 |
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