ES2571865T3 - Acilasas de beta-lactama de tipo II mutantes - Google Patents
Acilasas de beta-lactama de tipo II mutantesInfo
- Publication number
- ES2571865T3 ES2571865T3 ES14173437T ES14173437T ES2571865T3 ES 2571865 T3 ES2571865 T3 ES 2571865T3 ES 14173437 T ES14173437 T ES 14173437T ES 14173437 T ES14173437 T ES 14173437T ES 2571865 T3 ES2571865 T3 ES 2571865T3
- Authority
- ES
- Spain
- Prior art keywords
- seq
- acylase
- type
- beta
- lactam
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108700023418 Amidases Proteins 0.000 title abstract 6
- 102000005922 amidase Human genes 0.000 title abstract 6
- 150000003952 β-lactams Chemical class 0.000 title abstract 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 abstract 1
- 241000131407 Brevundimonas Species 0.000 abstract 1
- 241000589539 Brevundimonas diminuta Species 0.000 abstract 1
- 239000004471 Glycine Substances 0.000 abstract 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 abstract 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 abstract 1
- 241000589516 Pseudomonas Species 0.000 abstract 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 abstract 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 abstract 1
- 239000004473 Threonine Substances 0.000 abstract 1
- 150000001413 amino acids Chemical group 0.000 abstract 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Una acilasa de beta-lactama de tipo II mutante seleccionada del grupo que consiste en la acilasa SE83-acil de Pseudomonas según se representa en la SEQ ID NO: 1, N176 de Brevundimonas diminuta según se representa en la SEQ ID NO: 2 y la acilasa V22 de Brevundimonas diminuta según se representa en la SEQ ID NO: 3, y polipéptidos con actividad de acilasa de beta-lactama de tipo II que tienen una secuencia de aminoácidos que es al menos un 90% idéntica a lo largo de toda su longitud con SEQ ID NO: 1, SEQ ID NO: 2 o SEQ ID NO: 3, en donde dicha acilasa de beta-lactama de tipo II mutante se caracteriza por que leucina en la posición 161 ha sido modificada en glicina, serina o treonina.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05113024 | 2005-12-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2571865T3 true ES2571865T3 (es) | 2016-05-27 |
Family
ID=36584602
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES14173437T Active ES2571865T3 (es) | 2005-12-28 | 2006-12-28 | Acilasas de beta-lactama de tipo II mutantes |
Country Status (6)
| Country | Link |
|---|---|
| EP (2) | EP2851423B1 (es) |
| KR (1) | KR20080084986A (es) |
| CN (1) | CN101351549B (es) |
| BR (1) | BRPI0621278A2 (es) |
| ES (1) | ES2571865T3 (es) |
| WO (1) | WO2007073947A2 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103060298B (zh) * | 2012-12-31 | 2014-07-02 | 安徽丰原基因工程技术有限公司 | 一种头孢菌素c酰化酶突变体及其编码基因与应用 |
| KR101985911B1 (ko) * | 2017-12-28 | 2019-06-04 | 아미코젠주식회사 | Achromobacter sp. CCM 4824 유래 페니실린 G 아실라제 변이체 및 이의 이용 |
| CN109055475A (zh) * | 2018-08-03 | 2018-12-21 | 菏泽睿智科技开发有限公司 | 一种粪产碱杆菌的新用途 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4003894A (en) | 1971-08-17 | 1977-01-18 | Gist-Brocades N.V. | Preparation of 7-substituted amino-desacetoxycephalosporanic acid compounds |
| IE910956A1 (en) | 1990-03-23 | 1991-09-25 | Gist Brocades Nv | A method of modulating the production of secondary¹metabolites |
| WO1991016435A1 (en) | 1990-04-18 | 1991-10-31 | Gist-Brocades N.V. | Mutated beta-lactam acylase genes |
| US5318896A (en) | 1991-09-11 | 1994-06-07 | Merck & Co., Inc. | Recombinant expandase bioprocess for preparing 7-aminodesacetoxy cephalosporanic acid (7-ADCA) |
| GB9204439D0 (en) * | 1992-02-27 | 1992-04-15 | Fujisawa Pharmaceutical Co | A new cephalosporin c acylase |
| CN1087780C (zh) | 1993-07-30 | 2002-07-17 | 吉斯特·布罗卡迪斯公司 | 通过3－(羧乙硫基)丙酰－7－adca有效生产7－adca的方法 |
| WO1995004148A1 (en) | 1993-07-30 | 1995-02-09 | Gist-Brocades B.V. | Process for the efficient production of 7-adca via 2-(carboxyethylthio)acetyl-7-adca and 3-(carboxymethylthio)propionyl-7-adca |
| TW400384B (en) | 1993-11-01 | 2000-08-01 | Fujisawa Pharmaceutical Co | A new cephalosporin C acylase |
| GB9424945D0 (en) * | 1994-12-09 | 1995-02-08 | Fujisawa Pharmaceutical Co | A new cephalosporin C acylase |
| ATE417100T1 (de) | 1995-07-18 | 2008-12-15 | Dsm Ip Assets Bv | Eine verbesserte immobilisierte penicillin g acylase |
| ZA983396B (en) | 1997-04-22 | 1998-10-27 | Gist Brocades Bv | A method for controlling the solubility of a beta-lactam nucleus |
| CN1795197B (zh) | 2003-05-28 | 2010-06-02 | 帝斯曼知识产权资产管理有限公司 | 头孢菌素类化合物 |
| KR100530299B1 (ko) | 2003-08-11 | 2005-11-22 | 산도즈 게엠베하 | 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법 |
| ITMI20040016A1 (it) | 2004-01-12 | 2004-04-12 | Antibiotics S P A | Cefalosporina c acilasi |
-
2006
- 2006-12-28 ES ES14173437T patent/ES2571865T3/es active Active
- 2006-12-28 EP EP14173437.6A patent/EP2851423B1/en not_active Not-in-force
- 2006-12-28 CN CN2006800501155A patent/CN101351549B/zh active Active
- 2006-12-28 KR KR1020087015507A patent/KR20080084986A/ko not_active Withdrawn
- 2006-12-28 BR BRPI0621278-6A patent/BRPI0621278A2/pt not_active Application Discontinuation
- 2006-12-28 EP EP06841245A patent/EP1974029A2/en not_active Withdrawn
- 2006-12-28 WO PCT/EP2006/012654 patent/WO2007073947A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CN101351549A (zh) | 2009-01-21 |
| CN101351549B (zh) | 2013-03-20 |
| EP1974029A2 (en) | 2008-10-01 |
| EP2851423A1 (en) | 2015-03-25 |
| BRPI0621278A2 (pt) | 2011-12-06 |
| HK1128305A1 (en) | 2009-10-23 |
| KR20080084986A (ko) | 2008-09-22 |
| WO2007073947A3 (en) | 2007-11-08 |
| EP2851423B1 (en) | 2016-03-02 |
| WO2007073947A2 (en) | 2007-07-05 |
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