ES2644883T3 - Enzimas y métodos para producir ácidos grasos omega-3 - Google Patents

Enzimas y métodos para producir ácidos grasos omega-3 Download PDF

Info

Publication number
ES2644883T3
ES2644883T3 ES09827035.8T ES09827035T ES2644883T3 ES 2644883 T3 ES2644883 T3 ES 2644883T3 ES 09827035 T ES09827035 T ES 09827035T ES 2644883 T3 ES2644883 T3 ES 2644883T3
Authority
ES
Spain
Prior art keywords
dha
desaturase
fatty acids
total
epa
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES09827035.8T
Other languages
English (en)
Inventor
James Robertson Petrie
Anne Maree Mackenzie
Qing Liu
Pushkar Shrestha
Peter David Nichols
Susan Irene Ellis Blackburn
Maged Peter Mansour
Stanley Suresh Robert
Dion Matthew Frederick Frampton
Xue-Rong Zhou
Surinder Pal Singh
Craig Christopher Wood
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commonwealth Scientific and Industrial Research Organization CSIRO
Grains Research and Development Corp
Original Assignee
Commonwealth Scientific and Industrial Research Organization CSIRO
Grains Research and Development Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42197742&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2644883(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Commonwealth Scientific and Industrial Research Organization CSIRO, Grains Research and Development Corp filed Critical Commonwealth Scientific and Industrial Research Organization CSIRO
Application granted granted Critical
Publication of ES2644883T3 publication Critical patent/ES2644883T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/006—Refining fats or fatty oils by extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/06—Antiasthmatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/02—Drugs for disorders of the urinary system of urine or of the urinary tract, e.g. urine acidifiers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/06—Antipsoriatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/24—Antidepressants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
    • A61P25/32—Alcohol-abuse
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/02—Nutrients, e.g. vitamins, minerals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/24—Drugs for disorders of the endocrine system of the sex hormones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/06—Antiarrhythmics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/12—Antihypertensives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19003—Linoleoyl-CoA desaturase (1.14.19.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01003—Long-chain-fatty-acid-CoA ligase (6.2.1.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Neurology (AREA)
  • Diabetes (AREA)
  • Neurosurgery (AREA)
  • Virology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Nutrition Science (AREA)
  • Rheumatology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Hematology (AREA)
  • Psychiatry (AREA)
  • Cardiology (AREA)

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8
imagen9
imagen10
imagen11
imagen12
imagen13
imagen14
imagen15
imagen16
imagen17
imagen18
imagen19
imagen20
imagen21
imagen22
imagen23
imagen24
"ácidos grasos poliinsaturados de cadena muy larga" se refieren al ácido graso en un estado libre (no esterificado) o en una forma esterificada tal como formando parte de un triglicérido, diacilglicérido, monoacilglicérido, unido a acil-CoA u otra forma unida. El ácido graso puede estar esterificado como un fosfolípido tal como en las formas de fosfatidilcolina (PC), fosfatidiletanolamina, fosfatidilserina, fosfatidilglicerol, fosfatidilinositol o difosfatidilglicerol. Por 5 consiguiente, los LC-PUFA pueden estar presentes como una mezcla de formas en el lípido de una célula o un aceite purificado o lípido extraído de células, tejidos u organismos. En formas de realización preferidas, la invención proporciona un aceite que comprende al menos un 75 % o 85 % de triacilgliceroles, siendo el resto otras formas de lípidos tales como los ya mencionados, donde al menos dichos triacilgliceroles comprenden a los LC-PUFA. El aceite se puede purificar o tratar adicionalmente, por ejemplo, mediante hidrólisis con una base fuerte para liberar el ácido
10 graso libre, o mediante fraccionamiento, destilación o semejantes.
Las proteínas desaturasa, elongasa y acil transferasa, y los genes que codifican las mismas, que se pueden usar en la invención son cualquiera de los conocidos en el arte o bien, homólogos o derivados de los mismos. Los ejemplos de tales genes y los tamaños de las proteínas codificadas se enumeran en la Tabla 1. Las enzimas desaturasa cuya
15 participación en la biosíntesis de los LC-PUFA ha sido demostrada, pertenecen todas al grupo de las denominadas desaturasas “front-end”.
Tabla 1. Genes clonados relacionados con la biosíntesis de LC-PUFA
Enzima
Tipo de organismo Especie Nº Acceso Tamaño de la proteína (aa) Referencias
Δ4desaturasa
Protista Euglena gracilis AY278558 541 Meyer et al., 2003
Algas
Pavlova lutherii AY332747 445 Tonon et al., 2003
Isochrysis galbana
AAV33631 433 Pereira et al., 2004b
Pavlova salina
AAY15136 447 Zhou et al., 2007
Traustoquitriales
Thraustochytrium aureum AAN75707 AAN75708 AAN75709 AAN75710 515 N/D
Thraustochytrium sp. ATCC21685
AAM09688 519 Qiu et al. 2001
Δ5desaturasa
Mamíferos Homo sapiens AF199596 444 Cho et al., 1999b Leonard et al., 2000b
Nematodos
Caenorhabditis elegans AF11440, NM_069350 447 Michaelson et al., 1998b; Watts y Browse, 1999b
Hongos
Mortierella alpina AF067654 446 Michaelson et al., 1998a; Knutzon et al., 1998
Pythium irregulare
AF419297 456 Hong et al., 2002a
Dictyostelium discoideum
AB022097 467 Saito et al., 2000
Saprolegnia diclina
470 WO02081668
Diatomeas
Phaeodactilum tricornutum AY082392 469 Domergue et al., 2002
Algas
Thraustochytrium sp AF489588 439 Qiu et al., 2001
Thraustochytrium aureum
439 WO02081668
Isochrysis galbana
442 WO02081668
Musgo
Marchantia polimorpha AY583465 484 Kajikawa et al., 2004
Δ6desaturasa
Mamíferos Homo sapiens NM_013402 444 Cho et al., 1999a; Leonard et al., 2000
Mus musculus
NM_019699 444 Cho et al., 1999a
Nematodos
Caenorhabditis elegans Z70271 443 Napier et al., 1998
Plantas
Borago officinales U79010 448 Sayanova et al., 1997
Echium
AY055117 AY055118 Garcia-Maroto et al., 2002
26
Enzima
Tipo de organismo Especie Nº Acceso Tamaño de la proteína (aa) Referencias
Primula vialii
AY234127 453 Sayanova et al., 2003
Anemone leveillei
AF536525 446 Whitney et al., 2003
Musgos
Ceratodon purpureus AJ250735 520 Sperling et al., 2000
Marchantia polimorpha
AY583463 481 Kajikawa et al., 2004
Physcomitrella patens
CAA11033 525 Girke et al., 1998
Hongos
Mortierella alpina AF110510 AB020032 457 Huang et al., 1999; Sakuradani et al., 1999
Pythium irregulare
AF419296 459 Hong et al., 2002a
Mucor circinelloides
AB052086 467 NCBI*
Rhizopus sp.
AY320288 458 Zhang et al., 2004
Saprolegnia diclina
453 WO02081668
Diatomeas
Phaeodactilum tricornutum AY082393 477 Domergue et al., 2002
Bacteria
Synechocystis L11421 359 Reddy et al., 1993
Algas
Thraustochytrium aureum 456 WO02081668
Δ5/Δ6 desaturasa bifuncional
Peces Danio rerio AF309556 444 Hastings et al., 2001
C20 Δ8desaturasa
Algas Euglena gracilis AF139720 419 Wallis y Browse, 1999
Plantas
Borago officinales AAG43277 446 Sperling et al., 2001
Δ6-elongasa
Nematodos Caenorhabditis elegans NM_069288 288 Beaudoin et al., 2000
Musgos
Physcomitrella patens AF428243 290 Zank et al., 2002
Marchantia polimorpha
AY583464 290 Kajikawa et al., 2004
Hongos
Mortierella alpina AF206662 318 Parker-Barnes et al., 2000
Algas
Pavlova lutheri** 501 WO 03078639
Thraustochytrium
AX951565 271 WO 03093482
Thraustochytrium sp**
AX214454 271 WO 0159128
PUFAelongasa
Mamíferos Homo sapiens AF231981 299 Leonard et al., 2000b; Leonard et al., 2002
Rattus norvegicus
AB071985 299 Inagaki et al., 2002
Rattus norvegicus**
AB071986 267 Inagaki et al., 2002
Mus musculus
AF170907 279 Tvrdik et al., 2000
Mus musculus
AF170908 292 Tvrdik et al., 2000
Peces
Danio rerio AF532782 291 (282) Agaba et al., 2004
Danio rerio**
NM_199532 266 Lo et al., 2003
Gusanos
Caenorhabditis elegans Z68749 309 Abbott et al 1998 Beaudoin et al 2000
Algas
Thraustochytrium aureum** AX464802 272 WO 0208401-A2
Pavlova lutheri**
320 WO 03078639
Δ9-elongasa
Algas Isochrysis galbana AF390174 263 Qi et al., 2002
Euglena gracilis
258 WO 08/128241
Δ5-elongasa
Algas Ostreococcus tauri AAV67798 300 Meyer et al., 2004
Pyramimonas cordata
268 este trabajo
Pavlova sp. CCMP459
AAV33630 277 Pereira et al., 2004b
Pavlova salina
AAY15135 302 Robert et al., 2009
27
imagen25
imagen26
imagen27
imagen28
imagen29
imagen30
imagen31
imagen32
imagen33
imagen34
imagen35
imagen36
imagen37
imagen38
imagen39
imagen40
imagen41
imagen42
imagen43
imagen44
imagen45
imagen46
imagen47
imagen48
imagen49
imagen50
imagen51
imagen52
imagen53
imagen54
imagen55
imagen56
imagen57
5
10
15
20
25
30
35
El análisis por BLASTP usando la secuencia de aminoácidos de la desaturasa de Micromonas CCMP1545 SEQ ID NO:8 como búsqueda para otras proteínas en el banco de datos Genbank mostró que la proteína tuvo una homología con las ∆6-desaturasas. El mayor grado de identidad fue 66 % respecto de la longitud completa con la secuencia de aminoácidos de número de acceso AAW70159, la secuencia de una ∆6-desaturasa de Ostreococcus tauri. En la Figura 6 se provee un árbol de relación de secuencia basándose en el alineamiento múltiple de secuencias similar a la desaturasa de Micromonas CCMP1545. Esta desaturasa front-end contiene un dominio de citocromo b5 (dominio conservado pfam00173 en NCBI) en los aminoácidos entre 54 y 104 y el dominio conservado de similar a ∆6-FADS (Dominio conservado cd03506 en NCBI) en los aminoácidos entre 172 y 428. Las tres cajas de histidina indicativas de una desaturasa front-end están presentes en esta secuencia en 190-195, 227-232 y 401405, respectivamente. Las proteínas que contienen ambos de estos dominios son generalmente desaturasas frontend requeridas para la síntesis de ácidos grasos altamente insaturados. Interesantemente, estas desaturasas se agrupan estrechamente con AAW70159, la única desaturasa de acil-CoA similar a plantas bioquímicamente confirmada publicada hasta la fecha.
Caracterización funcional de la ∆6-desaturasa de Micromonas CCMP1545 en células de levaduras
La región codificante entera de la desaturasa de Micromonas, contenida en un fragmento KpnI-SacI del plásmido pGA4 se insertó en el vector para levaduras pYES2 en el sitio KpnI-SacI, generando pYES2+Micd6D para la introducción y caracterización funcional en levaduras. Las células de la cepa INVSC1 de levaduras se transformaron con pYES2+Micd6D y los transformantes se seleccionaron en medio sin uracilo. Las células de levaduras conteniendo pYES2+Micd6D se hicieron crecer en cultivo y luego se indujeron con galactosa. Luego de la adición de 0,5 mM de LA, ALA, ETrA, DGLA o ETA al medio de cultivo y 48 horas de cultivo adicional a 30oC, se analizaron los ácidos grasos en los lípidos celulares totales. Cuando se agregó LA al medio, la presencia de GLA en los lípidos celulares de las levaduras transformantes se detectó como el 3,9 % de FA totales, representando una eficacia de conversión ∆6-desaturación del 11,4 %. Cuando se agregó ALA al medio, la presencia de SDA en los lípidos celulares de las levaduras transformantes se detectó como el 13,9 % de FA totales, representando una eficacia de conversión ∆6-desaturación del 39,0 %. Esto es, la eficacia de conversión para sustratos ácidos grasos ω3 fue 3,5 veces mayor que para el correspondiente sustrato ácido graso ω6. Cuando se agregó ETrA al medio, la presencia de ETA en los lípidos celulares de las levaduras transformantes se detectó como el 0,21 % de FA totales, representando una eficacia de conversión ∆8-desaturación del 8,0 %. Sin embargo, cuando se agregó tanto DGLA o ETA al medio, la presencia de ARA o EPA, respectivamente, no se detectó. Esto indicó la ausencia de cualquier actividad de ∆5-desaturación (Tabla 7).
Tabla 7.Conversión de ácidos grasos en células de levaduras transformadas con construcciones genéticas que
expresan desaturasas aisladas de Micromonas CCMP1545, Ostreococcus lucimarinus y Pyramimonas CS-0140.
Clon
Precursor de ácido graso / % de FA totales Ácido graso formado / % de FA totales Tasa de conversión
pYES2+ Mic-d6D
LA, 18:2ω6 / 30,3 % GLA, 18:3ω6 / 3,9 % 11,4 %
pYES2+ Mic-d6D
ALA, 18:3ω3 / 21,7 % SDA, 18:4ω3 / 13,9 % 39,0 %
pYES2+ Mic-d6D
ETrA, 20:3ω3 / 2,4 % ETA, 20:4ω3 / 0,21 % 8,0 %
pYES2+ Mic-d6D
DGLA, 20:3ω6 / 2,6 % ARA, 20:4ω6 / 0 % -
pYES2+ Mic-d6D
ETA, 20:4ω3 / 6,2 % EPA, 20:5ω3 / 0 % -
pYES2+ Ostlu-d6D
LA, 18:2ω6 / 29,5 % GLA, 18:3ω6 / 2,1 % 6,6 %
pYES2+ Ostlu-d6D
ALA, 18:3ω3 / 21,8 % SDA, 18:4ω3 / 13,8 % 38,8 %
pYES2+ Ostlu-d6D
ETrA, 20:3ω3 / 2,2 % ETA, 20:4ω3 / 0 % -
pYES2+ Ostlu-d6D
GLA, 18:3ω6 / 29,2 % 18:4ω6 / 0 % -
pYES2+ Ostlu-d6D
SDA, 18:4ω3 / 41,7 % 18:5ω3 / 0 % -
pYES2+ Ostlu-d6D
DGLA, 20:3ω6 / 2,3 % ARA, 20:4ω6 / 0 % -
pYES2+ Ostlu-d6D
ETA, 20:4ω3 / 4,9 % EPA, 20:5ω3 / 0 % -
pYES2+ Pyrco-d5D
LA, 18:2ω6 / 35,1 % GLA, 18:3ω6 / 0 % -
61
imagen58
imagen59
imagen60
imagen61
imagen62
imagen63
imagen64
imagen65
imagen66
imagen67
imagen68
18:3 ω3*
0,0 0 0
18:1 ω9
21,4 13,9 59,5
18:1 ω7
3,3 3 7,9
18:0
4,3 8,5 14,7
20:4 ω6
21,0 6,7 19,1
20:5 ω3
1,3 7,1 22,2
20:3 ω6
1,1 0,3 1,5
20:4 ω3
0,2 0,5 2,8
20:2 ω6
2,6 0,4 1,8
20:3 ω3*
0,2 0 0
20:1 ω9/ ω11
1,6 2,2 21,1
20:0
0,7 0,4 4,2
22:4 ω6
0,8 1 4,4
22:5 ω3
0,0 2,4 14,9
22:0
0,0 0,2 0,7
24:1 ω9/11/13*
0,2 0 0
24:0
0,2 0,2 1,6
Suma de PUFA ω6
56 10 41
% de conversiones
∆9E
LA-->20:2 ω6
45 82
ALA-->20:3 ω3
100 100
∆8D
20:2ω6-->20:3 ω6
90 95
20:3ω3-->20:4 ω3
88 100
∆5D
20:3ω6-->20:4 ω6
95 96
20:4ω3-->20:5 ω3
90 95
Vale la pena señalar que la ∆8-desaturasa de Pavlova salina fue considerablemente más eficiente en la conversión de ETrA a ETA en comparación con las otras ∆8-desaturasas publicadas, en particular cuando se coexpresa con ∆9 elongasa y ∆5 desaturasa. Por ejemplo, se ha informado que cuando la ∆8-desaturasa de Euglena gracilis se
5 coexpresó o bien con la ∆9-elongasa de Euglena gracilis o bien con la de Isochrysis galbana en embriones de soja, las eficiencias de conversión de sustratos ω3y ω6 fueron 64 % y 73 %, respectivamente. La eficiencia de cada paso observada en el experimento que se describió previamente y la eficiencia de conversión total de ALA a EPA fue también mucho mayor que la informada por Qi et al. (2004) en hojas de Arabidopsis, en donde los mismos observaron solo 3,0 % de EPA y niveles sustanciales de intermediarios indeseables que incluyen ETrA (4,6 %).
10 Las elongasas se sabe que solo tienen acceso a sustratos en el conjunto de los acil-CoA. El hecho de que los subsiguientes pasos de la ∆8-desaturasa y la ∆5-desaturasa se hayan observado como funcionando con eficiencias extremadamente altas en las semillas transformadas, aun cuando el producto ∆9-elongado fue indudablemente producido en el conjunto de los acil-CoA, fue una fuerte indicación de que ambas desaturasas de Pavlova salina
15 eran capaces de acceder a sustratos acil-CoA con altas eficiencias.
Biosíntesis de altos niveles de ARA y EPA usando la ruta metabólica de ∆9-elongasa
A partir de estos datos y las observaciones de la eficiencia de los pasos individuales, se predijo que sería posible
20 generar altos niveles de ARA y EPA y subsiguientemente de DPA y DHA en una planta transgénica tal como Arabidopsis, canola, soja, lino o algodón usando una ruta metabólica de ∆9-elongasa modificada. Además se predijo que se pueden hacer niveles incluso más altos con agregado adicional de cualquiera de las tres funciones enzimáticas, o sea con la función acil-CoA ∆12-desaturasa, para incrementar la cantidad de sustrato LA disponible en el conjunto de los acil-CoA para la ∆9-elongación, segundo con el agregado de una ∆15-desaturasa para
25 incrementar el nivel de ALA para la conversión directa a EPA, y tercero con una ∆17-desaturasa que puede convertir
73
imagen69
imagen70
imagen71
imagen72
imagen73
imagen74
imagen75
5
10
15
20
25
30
0804673_Pyrco-elo1_pGA18, contenida en un fragmento SwaI, en 35S-pORE04 en el sitio SmaI-EcoRV, generando pJP2060. Se hizo una construcción genética 35S:Pavsa-d5D que codifica Δ5-desaturasa mediante la inserción de la región codificante completa de 0804674_Pavsa-d5D_pGA15, contenida en un fragmento SwaI, en 35S-pORE04 en el sitio SmaI-EcoRV, generando pJP2067. Se hizo una construcción genética 35S:Pyrco-d5E que codifica Δ5elongasa mediante la inserción de la región codificante completa de 0804675_Pyrco-elo2_pGA4, contenida en un fragmento SwaI, en 35S-pORE04 en el sitio SmaI-EcoRV, generando pJP2061. Se hizo una construcción genética 35S:Pavsa-d4D que codifica Δ4-desaturasa mediante la inserción de la región codificante completa de 0804676_Pavsa-d4D_pGA15, contenida en un fragmento SwaI, en 35S-pORE04 en el sitio SmaI-EcoRV, generando pJP2068. Se hizo una construcción genética 35S:Arath-DGAT1 que codifica la enzima DGAT1 mediante la inserción de la región codificante completa de pXZP513E, contenida en un fragmento BamHI-EcoRV, en 35S-pORE04 en el sitio BamHI-EcoRV, generando pJP2078.
Estos vectores quiméricos se introdujeron en forma individual en Agrobacterium tumefaciens cepa AGL1 y se mezclaron las células transgénicas de los cultivos de estas y se infiltró la mezcla en el tejido de hojas de plantas de Nicotiana benthamiana en el invernadero. Se hicieron crecer las plantas durante cinco días adicionales luego de la infiltración antes de tomar discos de hoja para análisis por GC lo que reveló que estos genes estaban funcionando para producir DHA en Nicotiana benthamiana. El tejido de hoja transformado con estos genes contuvo SDA (2,3 %), ETA (0,7 %), EPA (0,8 %), DPA (2,8 %) y DHA (4,4 %) (Tabla 14). El tejido de hoja también contuvo niveles traza de GLA, ETA y ARA. Las eficiencias de conversión fueron como sigue: 17,4 % del ALA que se produjo en la célula se convirtió a EPA (incluyendo EPA subsiguientemente convertido a DPA o DHA); 15,5 % del ALA se convirtió a DPA o DHA; 9,6 % del ALA que se produjo en la célula se convirtió a DHA; mientras que 40 % del ALA que se produjo en la célula que estaba ∆6-desaturó subsiguientemente se convirtió a DHA. Debido a la expresión transitoria de los transgenes en este experimento, se esperarían mayores eficiencias que las anteriores en células transformadas en forma estable.
Cuando se fraccionaron los lípidos totales extraídos de tejido de hoja mediante TLC para separar las clases lipídicas, y se analizaron las fracciones de TAG y de lípidos polares para composición de ácidos grasos por FAME, se observó que el nivel de DHA en los TAG era 7 % como porcentaje de los ácidos grasos totales, y en los lípidos polares el nivel de DHA era de 2,8 %. El menor nivel en la clase de lípidos polares se cree que fue debido a la contribución relativa de los lípidos de cloroplasto en las hojas, favoreciendo a los lípidos polares, y a la expresión transitoria de los genes en lugar de la inserción estable de los transgenes en el genoma de las células hospedadoras.
Tabla 14. Composición de ácidos grasos de lípidos de hojas transformadas con una combinación de desaturasas y elongasas.
Ácido graso
Ácido graso
Células no transformadas Células transformadas
16:0
palmítico 17,1 20,4
16:1d7
0,1 0,4
16:1d9
0,2 0,2
C6:1d?
0,5 0,4
16:1d?
0,5 0,4
17:1d9
0,9 0,7
16:2
0,9 0,9
16:3
6,6 5,4
18:0
esteárico 2,1 3,6
18:1d7
0,0 0,0
18:1d9
oleico 0,8 2,6
18:1d11
0,0 0,0
18:1d12
0,3 0,6
18:1d13
0,2 0,2
18:2n6
LA 5,1 10,8
18:3n6
GLA 0,7 1,9
18:3n3
ALA 57,8 35,0
20:0
0,4 0,7
20:1d5
0,4 0,3
18:4n3
SDA 0,4 2,3
20:1d8
0,0 0,0
20:1d11
0,0 0,0
20:2n6
EDA 0,1 0,2
20:3n6
DGLA 0,3 0,4
81
imagen76
Cuando se fraccionaron los lípidos totales extraídos de tejido de hoja mediante TLC para separar las clases lipídicas, y se analizaron las fracciones de TAG y de lípidos polares para composición de ácidos grasos por FAME, se observó que el nivel de DHA en los TAG era de 15,9 % como porcentaje de los ácidos grasos totales, y en los lípidos polares el nivel de DHA era de 4,4 %. El menor nivel en la clase de lípidos polares se cree que fue debido a la contribución relativa de los lípidos de cloroplasto en las hojas, favoreciendo a los lípidos polares, y a la expresión transitoria de los genes en lugar de la inserción estable de los transgenes en el genoma de las células hospedadoras.

Tabla 15. Composición de ácidos grasos de lípidos de hojas transformadas con una combinación de desaturasas y elongasas.
Control
Ruta Metabólica Pavsa-D5E DHA Ruta Metabólica Pyrco-D5E DHA
Ácido graso
Lípidos Totales Lípidos Totales TAG Lípidos Totales TAG
AG usuales
16:0
15,9±0,2 17,0±0,1 20,2 16,6±0,1 21,6
16:1∆3t
1,7±0,1 1,5±0,2 0,3 1,5±0,1 0,3
16:3 ∆9,12,15
6,3±0,3 5,2±0,1 0,4 5,6±0,1 0,6
18:0
3,6±0,3 3,5±0,1 5,9 3,3±0,1 6,5
18:1 ∆9
2,8±0,1 3,4±0,1 5,1 2,8±0,2 5,6
18:2 ∆9,12
18,7±0,1 14,1±0,4 15,2 13,0±0,1 17,3
18:3 ∆9,12,15
45,6±1,4 39,1±0,4 6,9 40,2±0,5 6,7
20:0
1,3±0,4 0,7±0 1,8 0,6±0 2,0
Otros minoritarios
4,1 2,5 6,3 2,3 6,2
Total
100 87,0 62,1 85,9 66,8
Nuevos PUFA w6
18:3 ∆6,9,12
- 1,6±0,1 3,3 2,1±0,2 4,3
20:3 ∆8,11,14
- - - - -
20:4 ∆5,8,11,14
- 0,3±0,1 0,5 0,2±0 -
Total
0 1,9 3,8 2,3 4,3
Nuevos PUFA ω3
18:4 ∆6,9,12,15
- 1,5±0,1 (22 % D6D) 3,8 2,0±0 (23 % D6D) 6,4
20:4 ∆8,11,14,17
- 0,5±0 (86 % D6E) 1,5 0,4±0 (83 % D6E) 1,6
20:5 ∆5,8,11,14,17
- 4,1±0,2 (95 % D5D) 14,2 0,7±0 (96 % D5D) 1,5
22:5 ∆7,10,13,16,19
- 2,4±0,1 (55 % D5E 1,6 4,3±0 (93 % D5E) 3,5
22:6 ∆4,7,10,13,16,19
- 2,6±0,1 (52 % D4D) 13,0 4,4±0,1 (51 % D4D) 15,9
Total
0 11,1 34,1 11,8 28,9
Total de nuevos ácidos grasos
0 13,0 37,9 14,0 33,2
Total de Ácidos grasos
100 100 100 100 100
Tabla 16. Eficiencias de conversión de hojas transformadas con una combinación de desaturasas y elongasas.
Ácido Graso
Total Enzima Eficiencia de Conversión
LA
13
GLA
2,1 15,0 % d6D
DGLA
0 8,7 % d6E
AA
0,2 100,0 % d5D
83 5
Ácido Graso
Total Enzima Eficiencia de Conversión
ALA
40,2
SDA
2 22,7 % d6D 18,1 % d6D a EPA+DPA+DHA
ETA
0,4 83,1 % d6E 16,7 % d6D a DPA+DHA
EPA
0,7 95,9 % d5D 8,5 % ALA a DHA
DPA
4,3 92,6 % d5E 46,8 % EPA a DHA
DHA
4,4 50,6 % d4D
TAG
LA
17,3
GLA
4,3 19,9 % d6D
DGLA
0 0,0 % d6E
AA
0 0,0 % d5D
ALA
6,7
SDA
6,4 81,2 % d6D 58,7 % d6D a EPA+DPA+DHA
ETA
1,6 77,9 % d6E 54,5 % d6D a DPA+DHA
EPA
1,5 92,9 % d5D 44,7 % ALA a DHA
DPA
3,5 92,8 % d5E 76,1 % EPA a DHA
DHA
15,9 82,0 % d4D
10
15
20
25
30
35
Discusión: Síntesis más eficiente de DHA en tejido de hoja y semilla
Este resultado pareciera facilitar el terreno para avances similares en el campo de los TAG de semilla debido a la sustancial conservación de los mecanismos extraplastídicos de síntesis de lípidos entre tejidos de hoja y semilla (Ohlrogge y Browse, 2004; Bates et al., 2007). Los inventores postulan que probablemente varios elementos sean responsables por este gran incremento de producción: 1. el uso de una acil-CoA ∆6-desaturasa específica para ω3 incrementa el flujo hacia la ruta metabólica para ω3 y disminuye la competición con sustratos ω6 paralelos por los subsiguientes pasos metabólicos; 2. una ∆5-elongasa altamente eficiente incrementa claramente la cantidad de DPA disponible para la ∆4-desaturación a DHA; 3. la reducción del silenciamiento génico mediante el uso de unidades transcripcionales independientes y el uso de una proteína supresora vírico (P19).
La fuerte preferencia hacia ω3 mostrada por la ∆6-desaturasa es claramente deseable cuando se intenta construir una planta de suelo que acumule los LC-PUFA ω3 EPA y DHA, debido a que la actividad ∆17-desaturasa adicional que se requeriría para convertir AA (20:4D5,8,11,14) a EPA no se requiere, simplificando de eso modo tanto la construcción metabólica como los desafíos regulatorios.
Además del paso optimizado previamente, el uso de la ∆5-elongasa de P. cordata altamente eficiente resultó en un perfil de ácidos grasos de la fracción TAG (aceite) que se asemeja mucho al aceite de atún, un aceite de pescado notable por su alto contenido en DHA y bajo contenido de intermediarios (Figura 18). Además, la actividad exhibida por la ∆5-elongasa de P. cordata en N. benthamiana es por lejos la ∆5-elongación más eficiente que hayan visto los inventores, y el uso de este gen supera en forma eficaz el gran cuello de botella de la ∆5-elongación que se ha experimentado en otros intentos de producción transgénica de DHA.
Finalmente, a pesar de que se requiere claramente el uso de genes óptimos, los inventores consideran probable que el método por el cual se introdujeron estos transgenes (o sea como casetes de expresión independiente y en presencia de un supresor de silenciamiento génico) jugó un importante rol para conseguir los altos niveles de DHA en este estudio. La manipulación metabólica para la producción de LC-PUFA se ha basado hasta ahora en construcciones multigénicas relativamente grandes que se insertan al azar en el genoma del huésped y, mientras muchos grupos han tenido buenos resultados con este método, existen indicaciones de que es difícil de obtener eventos que exhiban una misma expresión para todos los transgenes (WO 2004/017467). Además, los efectos de silenciamiento pueden reducir la eficiencia con el paso de generaciones (Matzke et al., 2001). Es posible que las estrategias de transformación alternativas tales como los cromosomas artificiales que involucran formación de centrómeros de novo sobre una unidad ensamblada en forma independiente y la manipulación de mini-cromosomas se puedan requerir finalmente para una exitosa manipulación metabólica estable de LC-PUFA (Yu et al., 2007).
84
imagen77
imagen78
imagen79
imagen80
imagen81
imagen82
células transgénicas de cultivos separados de AGL1 ya sea con pJP3057 o con 35S:LEC2 y se infiltró la mezcla en tejido de hoja de plantas de Nicotiana benthamiana. Se hicieron crecer las plantas por otros cuatro días luego de la infiltración antes de tomar discos de hoja para análisis por GC de los ácidos grasos totales en los lípidos de hoja, y en las fracciones lipídicas separadas. Esto reveló que estos genes estaban funcionando para producir DHA en
5 Nicotiana benthamiana (Tabla 19). El tejido de hoja transformado con estos genes contuvo SDA (1,2 %), ETA (2,0 %), EPA (0,6 %), DPA (1,7 %) y DHA (2,5 %). El tejido de hoja también contuvo GLA (2,4 %) y niveles traza de otros ácidos grasos ω6 de cadena larga.
Se introdujeron los vectores quiméricos pJP3115 y pJP3116 (Ejemplo 17) en Agrobacterium tumefaciens cepa
10 AGL1. Se mezclaron células transgénicas de cuatro cultivos separados de AGL1 con uno de pJP3115, pJP3116, 35S:P19 y 35S:LEC2 y se infiltró la mezcla en tejido de hoja de plantas de Nicotiana benthamiana. Se hicieron crecer las plantas por otros cuatro días luego de la infiltración antes de tomar discos de hoja para análisis por GC lo que reveló que estos genes estaban funcionando para producir DHA en Nicotiana benthamiana (Tabla 19). El tejido de hoja transformado con estos genes contuvo SDA (5,6 %), ETA (1,4 %), EPA (0,2 %), DPA (1,7 %) y DHA (2,4 %).
15 El tejido de hoja también contuvo niveles traza de ácidos grasos ω6 de cadena larga.
Este experimento confirmó que las construcciones duales pJP3115 y pJP3116 estaban funcionando en combinación para producir DHA en forma tan eficiente como una construcción individual que contiene los ocho genes.
20 Tabla 19. Composición de ácidos grasos (porcentaje de ácidos grasos totales) de tejido de hoja de Nicotiana benthamiana transformado en forma transitoria con diferentes construcciones. Los errores muestran la desviación estándar entre infiltraciones separadas llevadas a cabo por triplicado.
Ácidos grasos usuales
P19 sola 35S:LEC2 pJP3057 pJP3057 + 35S:LEC2
16:0
13,2 ± 0,5 12,8 ± 1,0 13,3 ± 0,1 13,2 ± 0,6
16:1∆3t
1,5 ± 0,1 1,4 ± 0,2 1,3 ± 0,1 1,1 ± 0,0
16:3 ∆ 9,12,15
7,6 ± 0,3 8,2 ± 0,4 7,1 ± 0,3 7,5 ± 0,4
18:0
1,7 ± 0,2 2,0± 0,4 1,8 ± 0,1 2,4 ± 0,3
18:1 ∆ 9
0,9 ± 0,1 0,9 ± 0,1 1,1 ± 0,1 1,5 ± 0,2
18:2 ∆ 9,12
12,6 ± 1,1 12,8 ± 0,6 13,8 ± 0,1 12,7 ± 0,4
18:3 ∆ 9,12,15
58,3 ± 1,9 56,3 ± 2,6 56,3 ± 0,7 44,8 ± 2,1
20:0
0,3 ± 0,0 0,4 ± 0,1 0,3 ± 0,0 0,5 ± 0,1
Otros minoritarios
3,8 4,6 3,9 4,8
Total
99,9 99,6 98,9 88,5
Nuevos PUFA ω6
18:3 ∆ 6,9,12
− 0,1 ± 0,0 − 2,4 ± 0,1
20:3 ∆ 8,11,14
0,1 ± 0,1 0,3 ± 0,1 0,2 ± 0,1 0,2 ± 0,1
20:4 ∆ 5,8,11,14
− − − −
22:4∆7,10,13,16
− − − 0,6 ± 0,1
22:5 ∆ 4,7,10,13,16
− − − 0,3 ± 0,0
Total
0,1 0,4 0,2 3,5
Nuevos PUFA ω3
18:4 ∆ 6,9,12,15
− − 0,9 ± 0,1 1,2 ± 0,1
20:4 ∆ 8,11,14,17
− − − 2,0 ± 0,1
20:5 ∆ 5,8,11,14,17
− − − 0,6 ± 0,0
22:5 ∆ 7,10,13,16,19
− − − 1,7 ± 0,1
22:6 ∆ 4,7,10,13,16,19
− − − 2,5 ± 0,2
Total
− − 0,9 8,0
Total de nuevos ácidos grasos
0,1 0,4 1,1 11,5
Total de ácidos grasos
100,0 100,0 100,0 100,0
Discusión: Verificación rápida de fallas y validación de construcciones específicas de semillas
Los experimentos que usan un factor de transcripción, en este caso LEC2, en combinación con un juego apropiado de genes, cada uno bajo el control de un promotor específico de tejido tal como un promotor específico de semilla, mostraron que dichas construcciones se pueden ensayar en un sistema heterólogo, tal como hojas, en donde el promotor específico de tejido normalmente no se expresaría, y siendo predictivos de la expresión en semillas. La
91
imagen83
imagen84
imagen85
imagen86
imagen87

Claims (1)

  1. imagen1
ES09827035.8T 2008-11-18 2009-11-17 Enzimas y métodos para producir ácidos grasos omega-3 Active ES2644883T3 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US19966908P 2008-11-18 2008-11-18
US199669P 2008-11-18
US27071009P 2009-07-09 2009-07-09
US270710P 2009-07-09
PCT/AU2009/001488 WO2010057246A1 (en) 2008-11-18 2009-11-17 Enzymes and methods for producing omega-3 fatty acids

Publications (1)

Publication Number Publication Date
ES2644883T3 true ES2644883T3 (es) 2017-11-30

Family

ID=42197742

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09827035.8T Active ES2644883T3 (es) 2008-11-18 2009-11-17 Enzimas y métodos para producir ácidos grasos omega-3

Country Status (14)

Country Link
US (12) US8809559B2 (es)
EP (2) EP3260544B1 (es)
JP (4) JP6068800B2 (es)
CN (5) CN113957105B (es)
AR (1) AR074364A1 (es)
AU (1) AU2009317860B2 (es)
BR (3) BRPI0921467B1 (es)
CA (3) CA2743880C (es)
CL (1) CL2011001162A1 (es)
DK (1) DK2358882T3 (es)
ES (1) ES2644883T3 (es)
NO (1) NO2358882T3 (es)
NZ (1) NZ593097A (es)
WO (1) WO2010057246A1 (es)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2166067B1 (de) 2003-08-01 2015-07-01 BASF Plant Science GmbH Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Organismen
US9458436B2 (en) 2004-02-27 2016-10-04 Basf Plant Science Gmbh Method for producing polyunsaturated fatty acids in transgenic plants
EP1720988B1 (de) 2004-02-27 2011-10-12 BASF Plant Science GmbH Verfahren zur herstellung von ungesättigten omega-3-fettsäuren in transgenen organismen
CN103451246B (zh) 2004-04-22 2018-02-16 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
CA3023314C (en) 2004-04-22 2019-12-10 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
AR059376A1 (es) 2006-02-21 2008-03-26 Basf Plant Science Gmbh Procedimiento para la produccion de acidos grasos poliinsaturados
CN105123532B (zh) 2006-07-14 2018-12-18 联邦科学技术研究组织 改变水稻的脂肪酸组成
EP2059588A4 (en) 2006-08-29 2010-07-28 Commw Scient Ind Res Org FATTY ACID SYNTHESIS
US20110218348A1 (en) * 2008-04-25 2011-09-08 Commonwealth Scientific Industrial Research Organisation Polypeptides and methods for producing triacylglycerols comprising modified fatty acids
US20110126325A1 (en) * 2008-04-25 2011-05-26 Commonwealth Scientific Industrial Research Organisation Recombinant cells and methods for hydroxylating fatty acids
CN102202498B (zh) 2008-07-21 2016-09-07 澳大利亚联邦科学与工业研究组织 改良的棉籽油及应用
CA2768737A1 (en) 2008-07-21 2010-01-28 Commonwealth Scientific And Industrial Research Organisation Improved vegetable oils and uses therefor
US8809559B2 (en) * 2008-11-18 2014-08-19 Commonwelath Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
CA3161358A1 (en) 2009-07-17 2011-01-20 Basf Plant Science Company Gmbh Novel fatty acid desaturases and elongases and uses thereof
ES2640100T3 (es) 2010-06-28 2017-10-31 Commonwealth Scientific And Industrial Research Organisation Métodos para producir lípidos
WO2012052468A2 (en) * 2010-10-21 2012-04-26 Basf Plant Science Company Gmbh Novel fatty acid desaturases, elongases, elongation components and uses therof
CA2853849C (en) * 2011-08-04 2015-10-13 Suntory Holdings Limited Protein exhibiting fatty acid elongation promoting activity, gene encoding same and use thereof
US8809026B2 (en) 2011-12-27 2014-08-19 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
US9347067B2 (en) 2011-12-27 2016-05-24 Commonwealth Scientific And Industrial Research Organisation Production of dihydrosterculic acid and derivatives thereof
US10822615B2 (en) 2011-12-27 2020-11-03 Commonwealth Scientific And Industrial Research Organisation Simultaneous gene silencing and suppressing gene silencing in the same cell
US11639507B2 (en) 2011-12-27 2023-05-02 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
CA3203397A1 (en) 2011-12-27 2013-07-04 Nuseed Global Innovation Ltd Processes for producing lipids
CA3148246A1 (en) * 2012-04-12 2013-10-17 Rothamsted Research Ltd Production of omega-3 long chain polyunsaturated fatty acids
CA2871503C (en) 2012-04-25 2023-08-29 Commonwealth Scientific And Industrial Research Organisation High oleic acid oils
BR122019026149A8 (pt) 2012-06-15 2022-06-28 Commw Scient Ind Res Org Célula hospedeira, processo de produzir um lipídio de planta extraído, método de produzir semente, farinha de semente, método para produzir um produto alimentício, uso e processo para produzir ésteres de etila
US20140090108A1 (en) * 2012-09-18 2014-03-27 Freydoun Garabagi Vectors and Methods For Enhancing Recombinant Protein Expression in Plants
GB201217524D0 (en) * 2012-10-01 2012-11-14 Rothamsted Res Ltd Recombinant organisms
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
CN103173445B (zh) * 2013-04-15 2014-07-30 南京江辉生物科技有限公司 一种欧米伽3的合成基因、含有合成基因的大肠菌及其构建方法
AU2014278053A1 (en) * 2013-06-14 2016-01-28 J.R. Simplot Company Protein production in plants
US11718577B2 (en) 2013-12-18 2023-08-08 Commonwealth Scientific And Industrial Research Organisation Lipid compositions comprising triacylglycerol with long-chain polyunsaturated fatty acids
PE20170253A1 (es) 2013-12-18 2017-04-14 Nuseed Pty Ltd Lipido que comprende acidos grasos poliinsaturados de cadena larga
EA037184B1 (ru) * 2014-06-27 2021-02-16 Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн Липид, содержащий докозапентаеновую кислоту
AU2015281791A1 (en) * 2014-06-27 2017-01-12 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
UA122770C2 (uk) * 2014-06-27 2021-01-06 Коммонвелз Сайнтіфік Енд Індастріал Рисерч Организейшн Ліпід, що містить докозапентаєнову кислоту
WO2016004473A1 (en) 2014-07-07 2016-01-14 Commonwealth Scientific And Industrial Research Organisation Processes for producing industrial products from plant lipids
WO2016075327A2 (en) 2014-11-14 2016-05-19 Basf Plant Science Company Gmbh Production of pufas in plants
US20180122021A1 (en) * 2015-03-03 2018-05-03 Uop Llc Chemical refinery performance optimization
JP2019129705A (ja) * 2016-03-31 2019-08-08 日本たばこ産業株式会社 植物に物質を導入する方法
ES2938626T3 (es) * 2016-05-03 2023-04-13 Lonza Ag Modulación del metabolismo de los lípidos para la producción de proteínas
AR109245A1 (es) * 2016-05-12 2018-11-14 Basf Plant Science Co Gmbh Métodos para optimizar la producción de metabolitos en plantas genéticamente modificadas y para procesar estas plantas
US12336552B2 (en) 2016-06-01 2025-06-24 Cargill, Incorporated Fish feed prepared from oilseed plants producing omega-3 fatty acids
EA201892783A1 (ru) 2016-06-16 2019-07-31 НЬЮСИД ПиТиВай ЛТД. Имбредная трансгенная линия канолы ns-b50027-4 и ее семена
US11859193B2 (en) 2016-09-02 2024-01-02 Nuseed Global Innovation Ltd. Plants with modified traits
EP3516066A1 (en) 2016-09-23 2019-07-31 Wake Forest University Modified microorganisms as sustainable sources of omega-3 polyunsaturated fatty acid production
US11913006B2 (en) 2018-03-16 2024-02-27 Nuseed Global Innovation Ltd. Plants producing modified levels of medium chain fatty acids
CN108220167A (zh) * 2018-04-10 2018-06-29 中盐工程技术研究院有限公司 盐藻纯化分离方法及微藻纯化分离方法
US10632211B2 (en) * 2018-06-11 2020-04-28 The Regents Of The University Of California Demethylation to treat eye disease
EP3586642A1 (en) 2018-06-21 2020-01-01 Nuseed Pty Ltd Ala enriched polyunsaturated fatty acid compositions
EP3586641A1 (en) 2018-06-21 2020-01-01 Nuseed Pty Ltd Dha enriched polyunsaturated fatty acid compositions
EP3586640A1 (en) 2018-06-21 2020-01-01 Nuseed Pty Ltd Dha enriched polyunsaturated fatty acid compositions
EP3586643A1 (en) 2018-06-21 2020-01-01 Nuseed Pty Ltd Dha enriched polyunsaturated fatty acid compositions
CA3132683A1 (en) 2019-03-08 2020-09-17 Commonwealth Scientific And Industrial Research Organisation Expression of nitrogenase polypeptides in plant cells
US10916099B2 (en) * 2019-06-04 2021-02-09 Igt Gaming system and method with guaranteed symbol generation
US10896579B2 (en) * 2019-06-04 2021-01-19 Igt Gaming system and method with dynamically determined guaranteed awards
CN110218777B (zh) * 2019-06-14 2023-05-16 苏州天合优才生物科技有限公司 Pcr预混液
TW202523283A (zh) * 2019-10-25 2025-06-16 美商努賽德營養美國股份有限公司 富集的多元不飽和脂肪酸組成物
WO2022015699A1 (en) * 2020-07-14 2022-01-20 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods to produce very long chain fatty acids
WO2022168958A1 (ja) 2021-02-05 2022-08-11 日本水産株式会社 超長鎖多価不飽和脂肪酸組成物
WO2023115116A1 (en) * 2021-12-20 2023-06-29 Commonwealth Scientific And Industrial Research Organisation Plants for producing cannabinoids
JP7809524B2 (ja) * 2022-01-11 2026-02-02 花王株式会社 脂質の製造方法
CN119614625A (zh) * 2025-02-17 2025-03-14 中国热带农业科学院三亚研究院 芜菁皱缩病毒蛋白p38在调控拟南芥双链结合蛋白上的应用

Family Cites Families (258)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
WO1984002913A1 (en) 1983-01-17 1984-08-02 Monsanto Co Chimeric genes suitable for expression in plant cells
US5504200A (en) 1983-04-15 1996-04-02 Mycogen Plant Science, Inc. Plant gene expression
ATE100141T1 (de) 1984-03-06 1994-01-15 Mgi Pharma Inc Herbizide resistenz in pflanzen.
US5420034A (en) 1986-07-31 1995-05-30 Calgene, Inc. Seed-specific transcriptional regulation
CA1338902C (en) 1986-02-27 1997-02-11 Howard M. Goodman Plant cells resistant to herbicidal glutamine synthetase inhibitors
MA20977A1 (fr) 1986-05-19 1987-12-31 Ciba Geigy Ag Plantes tolerant les herbicides contenant le gene de gluthathione S-Transferase
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
SE455438B (sv) 1986-11-24 1988-07-11 Aga Ab Sett att senka en brennares flamtemperatur samt brennare med munstycken for oxygen resp brensle
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
CN87100603A (zh) 1987-01-21 1988-08-10 昂科公司 抗黑素瘤疫苗
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US5932479A (en) 1988-09-26 1999-08-03 Auburn University Genetic engineering of plant chloroplasts
HU218465B (hu) 1989-08-09 2000-09-28 Dekalb Genetics Corporation Eljárások és kompozíciók stabilan transzformált, termő egyszikű növények és sejtjeik előállítására
ES2163392T3 (es) 1990-03-16 2002-02-01 Calgene Llc Nuevas secuencias expresadas preferentemente en el desarrollo de semillas precoces, y procedimientos relacionados con las mismas.
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5877402A (en) 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5861187A (en) 1990-08-30 1999-01-19 Cargill, Incorporated Oil from canola seed with altered fatty acid profiles and a method of producing oil
US7135614B1 (en) 1990-08-30 2006-11-14 Cargill, Incorporated Brassica or helianthus plants having mutant delta-12 or delta-15 sequences
US5518908A (en) 1991-09-23 1996-05-21 Monsanto Company Method of controlling insects
US6355861B1 (en) 1991-10-10 2002-03-12 Rhone-Poulenc Agrochimie Production of gamma linolenic acid by a Δ6-desaturase
US6683232B1 (en) 1991-10-10 2004-01-27 Rhone-Poulenc Agrochimie Production of γ linolenic acid by a Δ6-desaturase
US5614393A (en) 1991-10-10 1997-03-25 Rhone-Poulenc Agrochimie Production of γ-linolenic acid by a Δ6-desaturase
PH31293A (en) 1991-10-10 1998-07-06 Rhone Poulenc Agrochimie Production of y-linolenic acid by a delta6-desaturage.
JPH07501701A (ja) 1991-12-04 1995-02-23 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 植物由来の脂肪酸不飽和化酵素遺伝子
US5593874A (en) 1992-03-19 1997-01-14 Monsanto Company Enhanced expression in plants
US5683898A (en) 1992-05-15 1997-11-04 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
US5798259A (en) 1992-05-15 1998-08-25 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
CA2113557A1 (en) 1992-05-15 1993-11-25 Kazunaga Yazawa Gene which codes for eicosapentaenoic acid synthetase group and process for producing eicosapentaenoic acid
US6372965B1 (en) 1992-11-17 2002-04-16 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants
US6872872B1 (en) 1992-11-17 2005-03-29 E. I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
EP0688160B1 (en) 1993-03-11 1998-05-13 National Research Council Of Canada Enhanced regeneration system for cereals
CA2092588C (en) 1993-03-26 2008-07-08 Narender S. Nehra Enhanced regeneration system for cereals
US5362865A (en) 1993-09-02 1994-11-08 Monsanto Company Enhanced expression in plants using non-translated leader sequences
GB9324707D0 (en) 1993-12-02 1994-01-19 Olsen Odd Arne Promoter
GB9403512D0 (en) 1994-02-24 1994-04-13 Olsen Odd Arne Promoter
US5545818A (en) 1994-03-11 1996-08-13 Calgene Inc. Expression of Bacillus thuringiensis cry proteins in plant plastids
GB9515941D0 (en) 1995-08-03 1995-10-04 Zeneca Ltd DNA constructs
US5939288A (en) 1995-06-07 1999-08-17 Iowa State University Research Foundation, Inc. Plant secretory signal peptides and nectarins
EP0880312B1 (en) 1995-12-14 2006-03-08 Cargill Incorporated Plants having mutant sequences that confer altered fatty acid profiles
US7541519B2 (en) 1995-12-14 2009-06-02 Cargill, Incorporated Fatty acid desaturases and mutant sequences thereof
US6342658B1 (en) 1995-12-14 2002-01-29 Cargill, Incorporated Fatty acid desaturases and mutant sequences thereof
AU750363B2 (en) 1997-06-12 2002-07-18 Cargill Incorporated Fatty acid desaturases and mutant sequences thereof
EP0856060A2 (en) 1996-06-21 1998-08-05 Monsanto Company METHODS FOR THE PRODUCTION OF STABLY-TRANSFORMED, FERTILE WHEAT EMPLOYING $i(AGROBACTERIUM)-MEDIATED TRANSFORMATION AND COMPOSITIONS DERIVED THEREFROM
EP0913473A4 (en) 1996-07-10 2000-12-06 Sagami Chem Res METHOD FOR PRODUCING EICOSAPENTIC ACID BY GENETIC RECOMBINATION
US7109392B1 (en) 1996-10-09 2006-09-19 Cargill, Incorporated Methods for increasing oleic acid content in seeds from transgenic plants containing a mutant delta 12 desaturase
WO1998018952A1 (en) 1996-10-30 1998-05-07 Nippon Suisan Kaisha, Ltd. Process for producing fats containing highly unsaturated fatty acids containing selectively concentrated docosahexaenoic acid
US6194167B1 (en) 1997-02-18 2001-02-27 Washington State University Research Foundation ω-3 fatty acid desaturase
JP4127860B2 (ja) 1997-03-04 2008-07-30 サントリー株式会社 高度不飽和脂肪酸及びこれを含有する脂質の製造方法
US5977436A (en) 1997-04-09 1999-11-02 Rhone Poulenc Agrochimie Oleosin 5' regulatory region for the modification of plant seed lipid composition
US6051754A (en) 1997-04-11 2000-04-18 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US6428990B1 (en) 1997-04-11 2002-08-06 Abbott Laboratories Human desaturase gene and uses thereof
US6432684B1 (en) 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
US5968809A (en) 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US6075183A (en) 1997-04-11 2000-06-13 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
IL132148A0 (en) 1997-04-11 2001-03-19 Calgene Llc Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants
US7589253B2 (en) 1997-04-15 2009-09-15 Commonwealth Scientific And Industrial Research Organisation Fatty acid epoxygenase genes from plants and uses therefor in modifying fatty acid metabolism
US6235529B1 (en) 1997-04-29 2001-05-22 The Regents Of The University Of California Compositions and methods for plant transformation and regeneration
US6566583B1 (en) 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
US6140486A (en) 1997-06-04 2000-10-31 Calgene Llc Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants
WO1999005265A2 (en) 1997-07-23 1999-02-04 Sanford Scientific, Inc. Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance
WO1999014314A1 (en) 1997-09-12 1999-03-25 Commonwealth Scientific And Industrial Research Organisation Regulation of gene expression in plants
CA2305628C (en) 1997-09-30 2008-08-26 The Regents Of The University Of California Production of proteins in plant seeds
EP1037837A4 (en) 1997-10-01 2004-03-10 Busse Bros Inc MACHINING SYSTEM FOR CONTAINERS
GB9724783D0 (en) 1997-11-24 1998-01-21 Inst Arable Crops Research Novel polypeptides
US20030152983A1 (en) 1997-12-23 2003-08-14 University Of Bristol Desaturase
PL344868A1 (en) 1997-12-23 2001-11-19 Univ Bristol Desaturase
US6100447A (en) 1998-02-12 2000-08-08 Applied Phytologics, Inc. Method of barley transformation
US6838594B1 (en) 1998-03-20 2005-01-04 E. I. Du Pont De Nemours And Company Limnanthes oil genes
CA2319727C (en) 1998-03-20 2012-09-25 E.I. Du Pont De Nemours And Company Limnanthes oil genes
US6492108B1 (en) 1998-03-26 2002-12-10 Incyte Genomics, Inc. Delta-6 desaturase homologs
WO1999061602A1 (en) 1998-05-29 1999-12-02 Ohio University Compositions and methods for the synthesis of fatty acids, their derivatives and downstream products
DK1086236T3 (da) 1998-06-12 2007-12-03 Calgene Llc Polyumættede fedtsyrer i planter
AU4564399A (en) 1998-06-12 1999-12-30 Abbott Laboratories Polyunsaturated fatty acids in plants
EP1105486B1 (en) 1998-08-20 2011-04-27 E.I. Du Pont De Nemours And Company Genes for plant fatty acid modifying enzymes associated with conjugated double bond formation
AU5785199A (en) 1998-08-24 2000-03-14 Rutgers, The State University Of New Jersey Synthetic fatty acid desaturase gene for expression in plants
US20030163845A1 (en) 1998-09-02 2003-08-28 Pradip Mukerji Elongase genes and uses thereof
US6913916B1 (en) 1998-09-02 2005-07-05 Abbott Laboratories Elongase genes and uses thereof
US6677145B2 (en) 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
US6403349B1 (en) 1998-09-02 2002-06-11 Abbott Laboratories Elongase gene and uses thereof
WO2000020603A1 (en) 1998-10-05 2000-04-13 Abbott Laboratories Altered fatty acid biosynthesis in insect cells using delta five desaturase
WO2000020602A2 (en) 1998-10-05 2000-04-13 Abbott Laboratories Delta 6 and delta 12 desaturases and modified fatty acid biosynthesis and products produced therefrom
WO2000021557A1 (en) 1998-10-09 2000-04-20 Merck & Co., Inc. Delta 6 fatty acid desaturase
JP4182192B2 (ja) 1998-11-26 2008-11-19 独立行政法人産業技術総合研究所 イコサペンタエン酸及び／又はドコサヘキサエン酸の生合成に関与する酵素群をコードする遺伝子
US6864077B1 (en) 1998-12-03 2005-03-08 Edgar B. Cahoon Membrane-bound desaturases
JP2001095588A (ja) 1998-12-04 2001-04-10 Research Institute Of Innovative Technology For The Earth 緑藻のω３脂肪酸不飽和化酵素遺伝子及びその利用
US6825017B1 (en) 1998-12-07 2004-11-30 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
US7217856B2 (en) 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7247461B2 (en) 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US20070244192A1 (en) 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7271315B2 (en) 1999-01-14 2007-09-18 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7211418B2 (en) 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
AU3516100A (en) * 1999-03-05 2000-09-21 Monsanto Technology Llc Multigene expression vectors for the biosynthesis of products via multienzyme biological pathways
EP1035207A1 (en) 1999-03-09 2000-09-13 MultiGene Biotech GmbH cDNA molecules of the members of gene family encoding human fatty acid desaturases and their use in diagnosis and therapy
JP2002538826A (ja) 1999-03-18 2002-11-19 ザ ユニバーシティ オブ ブリストル カエノラブジチス・エレガンス由来の多飽和脂肪酸（ｐｕｆａ）エロンガーゼ
IL146782A0 (en) 1999-06-07 2002-07-25 Basf Plant Science Gmbh 6-acetylenase and 6-desaturase from ceratodon purpureus
AU776417B2 (en) 1999-07-06 2004-09-09 Basf Plant Science Gmbh Plants expressing delta6-desaturase genes and oils from these plants containing pufas and method for producing unsaturated fatty acids
WO2001004636A1 (en) 1999-07-12 2001-01-18 Ohio University Mammalian cells expressing desaturases and elongases
US7070970B2 (en) 1999-08-23 2006-07-04 Abbott Laboratories Elongase genes and uses thereof
US7067722B2 (en) 1999-08-26 2006-06-27 Monsanto Technology Llc Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
EP1206558B1 (en) 1999-08-26 2008-01-09 Calgene LLC Plants with modified polyunsaturated fatty acids
US7531718B2 (en) 1999-08-26 2009-05-12 Monsanto Technology, L.L.C. Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
DE10044468A1 (de) 1999-09-10 2001-03-15 Axiva Gmbh Neue Nukleinsäure aus Tetrahymena kodierend für eine delta-6Desaturase, ihre Herstellung und ihre Verwendung
FR2798391B1 (fr) 1999-09-15 2001-12-14 Inst Nat Sante Rech Med Enzyme thyroidienne nadph oxydase, acide nucleique codant pour cette enzyme et leurs applications
DE19950589A1 (de) 1999-10-20 2001-05-23 Gvs Ges Fuer Erwerb Und Verwer Elongasepromotoren für gewebespezifische Expression von Transgenen in Pflanzen
JP4221476B2 (ja) 1999-11-19 2009-02-12 独立行政法人産業技術総合研究所 イコサペンタエン酸生合成遺伝子群をクローニングしたプラスミド及びイコサペンタエン酸を産生するラン藻
EP1232274B1 (en) * 1999-11-22 2010-03-10 Plant Bioscience Limited Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs)
WO2001038541A1 (en) 1999-11-25 2001-05-31 Basf Plant Science Gmbh Moss genes from physcomitrella patents encoding proteins involved in the synthesis of polyunsaturated fatty acids and lipids
JP2001169780A (ja) 1999-12-15 2001-06-26 Natl Inst Of Advanced Industrial Science & Technology Meti ドコサヘキサエン酸生産細菌の遺伝子
GB9929897D0 (en) 1999-12-18 2000-02-09 Slabas Antoni R Improvements in or relating to conjugated fatty acids and related compounds
US20030172398A1 (en) 1999-12-21 2003-09-11 Browse John A. Novel delta-12 desaturase and methods of using it for synthesis of polyunsaturated fatty acids
PL207026B1 (pl) 2000-02-09 2010-10-29 Basf Ag Wyizolowany kwas nukleinowy, konstrukt genowy zawierający ten kwas, sekwencja aminokwasowa kodowana przez wyizolowaną sekwencję tego kwasu nukleinowego, wektor zawierający kwas nukleinowy lub konstrukt genowy, roślina transgeniczna lub mikroorganizm transgeniczny zawierający kwas nukleinowy, konstrukt genowy lub wektor, przeciwciało specyficznie wiążące się z polipeptydem kodowanym przez kwas nukleinowy, cząsteczka antysensownego kwasu nukleinowego oraz sposób wytwarzania wielonienasyconych kwasów tłuszczowych (PUFA)
US20020009779A1 (en) 2000-05-19 2002-01-24 Meyers Rachel A. 50365, a novel hexokinase family member and uses therefor
US6686185B1 (en) 2000-03-07 2004-02-03 Millennium Pharmaceuticals, Inc. 25934, a novel fatty acid desaturase and uses therefor
US7411054B2 (en) 2000-03-07 2008-08-12 Millennium Pharmaceuticals, Inc. 25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 and 49933 molecules and uses therefor
US20040157221A9 (en) 2000-03-07 2004-08-12 Millennium Pharmaceuticals, Inc. Novel 25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 and 49933 molecules and uses therefor
US6492577B1 (en) 2000-03-16 2002-12-10 The Regents Of The University Of California Leafy cotyledon2 genes and their uses
WO2001073060A2 (en) 2000-03-24 2001-10-04 Millennium Pharmaceuticals, Inc. 18221, dual specificity phosphatase and uses thereof
WO2002000859A2 (en) 2000-06-23 2002-01-03 Millennium Pharmaceuticals, Inc. 46508, a novel human peptidyl-trna hydrolase family member and uses thereof
CA2301158A1 (en) 2000-03-24 2001-09-24 Stephen J. Allen Screening methods for compounds useful for modulating lipid metabolism in disease
AU2000234588A1 (en) 2000-03-31 2001-10-15 Idemitsu Petrochemical Co. Ltd. Process for producing lipids and lipid-secreting microorganisms
WO2001079499A1 (en) 2000-04-18 2001-10-25 Commonwealth Scientific And Industrial Research Organisation Method of modifying the content of cottonseed oil
US7091005B2 (en) 2000-05-16 2006-08-15 Merck & Co., Inc. Gene responsible for Stargardt-like dominant macular dystrophy
WO2001087140A2 (en) 2000-05-16 2001-11-22 Crosetto Dario B Method and apparatus for anatomical and functional medical imaging
WO2001092489A2 (en) 2000-05-26 2001-12-06 Washington State University Research Foundation Palmitate desaturase gene
WO2001096363A1 (en) 2000-06-12 2001-12-20 Boyce Thompson Institute For Plant Research Fatty acid desaturase gene and protein for modulating activation of defense signaling pathways in plants
AU2001271732A1 (en) 2000-06-29 2002-01-14 Millennium Pharmaceuticals, Inc. 25869, a human carboxylesterase and uses thereof
EP1354049A2 (en) 2000-07-17 2003-10-22 Millenium Pharmaceuticals, Inc. 16816 and 16839, human phospholipase c molecules and uses therefor
GB0316629D0 (en) 2003-07-16 2003-08-20 Univ York Transgenic cell
EP1339839A2 (en) 2000-08-21 2003-09-03 Millennium Pharmaceuticals, Inc. Human transporter family members and uses thereof
DE60134995D1 (de) 2000-09-26 2008-09-04 Xenon Pharmaceuticals Inc Verfahren und zusammensetzungen, die eine stearoyl-coa desuturase-hscd5 verwenden
JP4071622B2 (ja) 2000-09-28 2008-04-02 バイオリジナル フード アンド サイエンス コーポレーション 脂肪酸不飽和化酵素ファミリーのメンバーであるｆａｄ４、ｆａｄ５、ｆａｄ５−２およびｆａｄ６、ならびにそれらの使用方法
US7297523B2 (en) 2000-11-29 2007-11-20 Xenon Pharmaceuticals Inc. Human elongase genes and uses thereof
DE10102337A1 (de) 2001-01-19 2002-07-25 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren, neue Biosynthesegene sowie neue pflanzliche Expressionskonstrukte
DE10102338A1 (de) 2001-01-19 2002-07-25 Basf Plant Science Gmbh Verfahren zur Expression von Biosynthesegenen in pflanzlichen Samen unter Verwendung von neuen multiplen Expressionskonstrukten
EP1392823B1 (en) 2001-01-25 2011-11-30 Abbott Laboratories Desaturase genes and uses thereof
US6635451B2 (en) 2001-01-25 2003-10-21 Abbott Laboratories Desaturase genes and uses thereof
DE10106660A1 (de) 2001-02-12 2002-08-29 Celanese Ventures Gmbh Verfahren zur Herstellung von gamma-Linolensäure aus einer Ciliaten-Kultur durch Zusatz geeigneter Vorläufermoleküle zu dem Kulturmedium
GB0107510D0 (en) 2001-03-26 2001-05-16 Univ Bristol New elongase gene and a process for the production of -9-polyunsaturated fatty acids
WO2002081702A1 (fr) 2001-04-05 2002-10-17 Idemitsu Technofine Co., Ltd. Genes de desaturase en position $g(d)6 d'acide gras, plasmides et transformants contenant ces genes
DE50209495D1 (de) 2001-04-11 2007-03-29 Bosch Gmbh Robert Handwerkzeugmaschine mit vibrationsgedämpftem handgriff
TWI337619B (en) 2001-04-16 2011-02-21 Martek Biosciences Corp Pufa polyketide synthase systems and uses thereof
TWI324181B (en) 2001-04-16 2010-05-01 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US7045683B2 (en) 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
US20030082754A1 (en) 2001-05-04 2003-05-01 Pradip Mukerji Delta4 - desaturase genes and uses thereof
AU2002309856B2 (en) * 2001-05-14 2008-10-23 Dsm Ip Assets B.V. Production and use of a polar lipid-rich fraction containing omega-3 and/or omega-6 highly unsaturated fatty acids from microbes, genetically modified plant seeds and marine organisms
EP1423521B1 (en) 2001-06-06 2008-08-13 Bioriginal Food & Science Corp. Flax (linum usitatissimum l.) seed-specific sequences
DE10134660A1 (de) 2001-07-20 2003-02-06 Basf Plant Science Gmbh Fettsäure-Desaturase-Gene aus Granatapfel und Verfahren zur Herstellung von ungesättigten Fettsäuren
US20050089879A1 (en) 2001-07-31 2005-04-28 Ivo Feussner Method for producing arachidonic acid in transgenic organisms
US6875595B2 (en) 2001-09-13 2005-04-05 Divergence, Inc. Nematode fatty acid desaturase-like sequences
JP2003116566A (ja) 2001-10-12 2003-04-22 Osamu Suzuki ｎ−３系ドコサペンタエン酸の製造方法
US7211656B2 (en) 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
AU2003206714B2 (en) 2002-01-30 2007-09-06 Basf Plant Science Gmbh Elongase gene and method for producing polyunsaturated fatty acids
GB2385852A (en) 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
US7700833B2 (en) 2002-03-01 2010-04-20 Cornell University Process for the production of unsaturated fatty acids
CA2519169C (en) 2002-03-16 2013-04-30 The University Of York Transgenic plants expressing enzymes involved in fatty acid biosynthesis
DE10219203A1 (de) 2002-04-29 2003-11-13 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in Pflanzen
BRPI0311324B1 (pt) 2002-05-22 2020-01-28 Monsanto Technology Llc polinucleotídeo, vetor, polipeptídeo com atividade dessasturase, métodos para produção de planta e óleo de milho contendo ácidos graxos de ômega-3, para aumentar o valor nutricional de um produto comestível, de fabricação de alimento ou alimentação, bem como composição comestível
WO2004005442A1 (de) 2002-07-03 2004-01-15 Basf Plant Science Gmbh Verfahren zur herstellung von konjugierten mehrfach ungesättigten fettsäuren mit mindestens zwei doppelbindungen in pflanzen
JP2006516348A (ja) 2002-08-19 2006-06-29 アンダーソン パワー プロダクツ 電気コネクタ用のハンドル固定システムおよびその方法
AU2003296638B2 (en) 2002-12-19 2009-06-11 University Of Bristol Method for the production of polyunsaturated fatty acids
GB0229578D0 (en) * 2002-12-19 2003-01-22 Univ Bristol Novel method for the production of polyunsaturated fatty acids
US8084074B2 (en) 2003-02-12 2011-12-27 E. I. Du Pont De Nemours And Company Production of very long chain polyunsaturated fatty acids in oil seed plants
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
ATE517984T1 (de) * 2003-02-27 2011-08-15 Basf Plant Science Gmbh Verfahren zur herstellung mehrfach ungesättigter fettsäuren
US7855321B2 (en) 2003-03-31 2010-12-21 University Of Bristol Plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
DE10315026A1 (de) 2003-04-02 2004-10-14 Bioplanta Arzneimittel Gmbh Zusammensetzungen enthaltend omega-3-fettsäurehaltige Öle und Pflanzenextrakte
WO2004090123A2 (de) 2003-04-08 2004-10-21 Basf Plant Science Gmbh Δ-4-desaturasen aus euglena gracilis, exprimierende pflanzen und pufa enthaltende öle
DE602004013569D1 (de) 2003-04-16 2008-06-19 Basf Plant Science Gmbh N pflanzen
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US8313911B2 (en) 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US20110059496A1 (en) 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
EP2166067B1 (de) * 2003-08-01 2015-07-01 BASF Plant Science GmbH Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Organismen
ATE430802T1 (de) 2003-08-21 2009-05-15 Monsanto Technology Llc Fettsäuredesaturasen aus primula
US7504259B2 (en) 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
EP1682566B1 (en) 2003-11-12 2013-06-05 E.I. Du Pont De Nemours And Company Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous plants and yeast
MY140210A (en) 2003-12-22 2009-11-30 Suntory Holdings Ltd Marchantiales-derived unsaturated fatty acid synthetase genes and use of the same
US7951994B2 (en) 2004-02-17 2011-05-31 The University Of York Desaturase enzymes
EP1720988B1 (de) 2004-02-27 2011-10-12 BASF Plant Science GmbH Verfahren zur herstellung von ungesättigten omega-3-fettsäuren in transgenen organismen
WO2005083093A2 (de) 2004-02-27 2005-09-09 Basf Plant Science Gmbh Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen
US9458436B2 (en) * 2004-02-27 2016-10-04 Basf Plant Science Gmbh Method for producing polyunsaturated fatty acids in transgenic plants
US7217956B2 (en) 2004-03-29 2007-05-15 Articulated Technologies, Llc. Light active sheet material
DE102004017369A1 (de) 2004-04-08 2005-11-03 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Screeningverfahren zur Identifizierung von PUFA-PKS in Proben
DE102004017370A1 (de) 2004-04-08 2005-10-27 Nutrinova Nutrition Specialties & Food Ingredients Gmbh PUFA-PKS Gene aus Ulkenia
CN1968688A (zh) 2004-04-16 2007-05-23 孟山都技术有限公司 脂肪酸去饱和酶在玉米中的表达
CA3023314C (en) 2004-04-22 2019-12-10 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
CN101018862B (zh) * 2004-04-22 2013-10-09 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
CN103451246B (zh) 2004-04-22 2018-02-16 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
AU2005235627B2 (en) 2004-04-22 2011-07-07 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
EP1766023B1 (en) 2004-06-04 2010-09-01 Fluxome Sciences A/S Metabolically engineered cells for the production of polyunsaturated fatty acids
WO2006012325A1 (en) 2004-06-25 2006-02-02 E.I. Dupont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
DE102004060340A1 (de) 2004-07-16 2006-02-09 Basf Plant Science Gmbh Verfahren zur Erhöhung des Gehalts an mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
US7270315B2 (en) 2004-09-28 2007-09-18 Amt International, Inc. Exchange column, perforated tray assembly
GB0421937D0 (en) 2004-10-02 2004-11-03 Univ York Acyl CoA synthetases
US7192762B2 (en) 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7198937B2 (en) 2004-11-04 2007-04-03 E. I. Du Pont De Nemours And Company Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US8685679B2 (en) 2004-11-04 2014-04-01 E I Du Pont De Nemours And Company Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms
US20060094102A1 (en) 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
US7273746B2 (en) 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7189559B2 (en) 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7588931B2 (en) 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
WO2006064317A1 (en) 2004-12-14 2006-06-22 Avestha Gengraine Technologies Pvt. Ltd. Recombinant production docosahexaenoic acid (dha) in yeast
DE102004063326A1 (de) 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Organismen
DE102004062294A1 (de) 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
DE102005013779A1 (de) 2005-03-22 2006-09-28 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten C20- und C22-Fettsäuren mit mindestens vier Doppelbindungen in transgenen Pflanzen
WO2006135866A2 (en) 2005-06-10 2006-12-21 Martek Biosciences Corporation Pufa polyketide synthase systems and uses thereof
WO2007005882A2 (en) * 2005-07-05 2007-01-11 North Carolina State University Methods and compositions for expressing proteins in plants
DE102005038036A1 (de) 2005-08-09 2007-02-15 Basf Plant Science Gmbh Verfahren zur Herstellung von Arachidonsäure und/oder Eicosapentaensäure in transgenen Nutzpflanzen
GB2431158A (en) 2005-10-13 2007-04-18 Rothamsted Res Ltd Process for the production of arachidonic and/or eicosapentaenoic acid
DE102005052551A1 (de) 2005-11-02 2007-05-16 Rothamsted Res Harpenden Verfahren zur Herstellung von y-Linolensäure und/oder Stearidonsäure in transgenen Brassicaceae und Linaceae
KR20080071190A (ko) 2005-11-23 2008-08-01 이 아이 듀폰 디 네모아 앤드 캄파니 델타-９ 일롱가제, 및 다중불포화 지방산 생성에 있어서의이들의 용도
CA2640473A1 (en) * 2006-02-03 2007-08-16 The Texas A & M University System Enhancing expression of value-added genes by transgenic expression of tombusvirus-based p19 gene mutants
GB0603160D0 (en) 2006-02-16 2006-03-29 Rothamsted Res Ltd Nucleic acid
AR059376A1 (es) 2006-02-21 2008-03-26 Basf Plant Science Gmbh Procedimiento para la produccion de acidos grasos poliinsaturados
KR101524398B1 (ko) 2006-03-15 2015-06-04 디에스엠 아이피 어셋츠 비.브이. Ｐｕｆａ 폴리케티드 신타제 시스템을 이용한 이종 생물체내 다불포화 지방산의 제조
US7943823B2 (en) 2006-04-28 2011-05-17 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
JP5172828B2 (ja) 2006-05-29 2013-03-27 アイコン・ジェネティクス・ゲーエムベーハー 植物ウイルスに基づいた誘導性発現システム
JP4230494B2 (ja) 2006-06-06 2009-02-25 日立建機株式会社 電気駆動ダンプトラックの駆動システム
CA2656786C (en) * 2006-07-11 2019-07-02 Bioriginal Food & Science Corporation Fatty acid desaturases and uses thereof
DE102006034313A1 (de) * 2006-07-21 2008-01-24 Basf Plant Science Gmbh Verfahren zur Herstellung von Arachidonsäure und/oder Eicosapentaensäure
EP2500420B1 (en) 2006-08-24 2016-06-22 BASF Plant Science GmbH Pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains
EP2059588A4 (en) 2006-08-29 2010-07-28 Commw Scient Ind Res Org FATTY ACID SYNTHESIS
PL2177605T3 (pl) * 2006-10-06 2015-05-29 Basf Plant Science Gmbh Delta-5 desaturazy i sposób wytwarzania wielokrotnie nienasyconych kwasów tłuszczowych w organizmach transgenicznych innych niż człowiek
US8916361B2 (en) 2006-11-17 2014-12-23 Abbott Laboratories Elongase gene and uses thereof
US7709239B2 (en) 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
WO2008104559A1 (de) * 2007-02-27 2008-09-04 Norddeutsche Pflanzenzucht Verfahren zur herstellung von mehrfach ungesättigten fettsäuren in transgenen organismen
MX2009010574A (es) 2007-04-03 2009-10-22 Du Pont Multienzimas y su uso en la fabricacion de acidos grasos poliinsaturados.
US7794701B2 (en) 2007-04-16 2010-09-14 E.I. Du Pont De Nemours And Company Δ-9 elongases and their use in making polyunsaturated fatty acids
US8957280B2 (en) 2007-05-03 2015-02-17 E. I. Du Pont De Nemours And Company Delta-5 desaturases and their use in making polyunsaturated fatty acids
ATE528412T1 (de) 2007-07-31 2011-10-15 Basf Plant Science Gmbh Desaturasen und verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen organismen
US8318914B2 (en) 2007-07-31 2012-11-27 Bioriginal Food & Science Corp. Elongases and methods for producing polyunsaturated fatty acids in transgenic organisms
US8119784B2 (en) 2008-04-02 2012-02-21 E. I. Du Pont De Nemours And Company Delta-4 desaturase and its use in making polyunsaturated fatty acids
JP2011519552A (ja) 2008-04-25 2011-07-14 ビーエーエスエフ プラント サイエンス ゲーエムベーハー 植物種子油
US20110218348A1 (en) 2008-04-25 2011-09-08 Commonwealth Scientific Industrial Research Organisation Polypeptides and methods for producing triacylglycerols comprising modified fatty acids
BRPI0911892A8 (pt) 2008-04-30 2018-06-26 Rothamsted Res Ltd polinucleotídeo, vetor, célula hospedeira, polipeptídeo e organismo não humano, transgênico, e uso dos mesmos, processo de geração de um polipeptídio com atividade de desaturase ou elongase, anticorpo e processo para a produção de uma composição de óleo, lipídio ou ácido graxo
DE112009001385T5 (de) 2008-06-03 2011-04-14 Basf Plant Science Gmbh Fettsäure-Dehydratasen und Anwendungen davon
US8168858B2 (en) 2008-06-20 2012-05-01 E. I. Du Pont De Nemours And Company Delta-9 fatty acid elongase genes and their use in making polyunsaturated fatty acids
AU2009265795B2 (en) 2008-07-01 2014-12-11 Basf Plant Science Gmbh Promoters from Brassica napus for seed specific gene expression
CA2768737A1 (en) 2008-07-21 2010-01-28 Commonwealth Scientific And Industrial Research Organisation Improved vegetable oils and uses therefor
CN102202498B (zh) 2008-07-21 2016-09-07 澳大利亚联邦科学与工业研究组织 改良的棉籽油及应用
JP5757870B2 (ja) 2008-08-26 2015-08-05 ビーエーエスエフ プラント サイエンス ゲーエムベーハー デサチュラーゼをコードする核酸および改変植物油
WO2010045324A1 (en) 2008-10-14 2010-04-22 Monsanto Technology Llc Utilization of fatty acid desaturases from hemiselmis spp.
US8809559B2 (en) 2008-11-18 2014-08-19 Commonwelath Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
EP2669380B1 (en) 2008-12-12 2017-09-27 BASF Plant Science GmbH Desaturases and process for the production of polyunsaturated fatty acids in transgenic organisms
US9212371B2 (en) 2009-05-13 2015-12-15 Basf Plant Science Company Gmbh Acyltransferases and uses thereof in fatty acid production
US8188335B2 (en) 2009-07-17 2012-05-29 Abbott Laboratories Δ9-elongase for production of polyunsaturated fatty acid-enriched oils
CA3161358A1 (en) 2009-07-17 2011-01-20 Basf Plant Science Company Gmbh Novel fatty acid desaturases and elongases and uses thereof
CA2772267A1 (en) 2009-08-31 2011-03-03 Basf Plant Science Company Gmbh Regulatory nucleic acid molecules for enhancing seed-specific gene expression in plants promoting enhanced polyunsaturated fatty acid synthesis
ES2640100T3 (es) 2010-06-28 2017-10-31 Commonwealth Scientific And Industrial Research Organisation Métodos para producir lípidos
US9311457B1 (en) 2011-11-02 2016-04-12 Google Inc. Platform for cloud application software
BR122019026149A8 (pt) 2012-06-15 2022-06-28 Commw Scient Ind Res Org Célula hospedeira, processo de produzir um lipídio de planta extraído, método de produzir semente, farinha de semente, método para produzir um produto alimentício, uso e processo para produzir ésteres de etila
PE20170253A1 (es) 2013-12-18 2017-04-14 Nuseed Pty Ltd Lipido que comprende acidos grasos poliinsaturados de cadena larga
AU2015281791A1 (en) 2014-06-27 2017-01-12 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid

Also Published As

Publication number Publication date
US20240150779A1 (en) 2024-05-09
US8809559B2 (en) 2014-08-19
BR122021003835B1 (pt) 2022-02-08
CN113957105B (zh) 2024-11-01
US20120016144A1 (en) 2012-01-19
US20240150781A1 (en) 2024-05-09
JP2019022493A (ja) 2019-02-14
EP2358882B1 (en) 2017-07-26
EP3260544A1 (en) 2017-12-27
NZ593097A (en) 2013-02-22
US12195739B2 (en) 2025-01-14
US20240150782A1 (en) 2024-05-09
CA2743880A1 (en) 2010-05-27
CN114045301A (zh) 2022-02-15
US20180087004A1 (en) 2018-03-29
EP2358882A1 (en) 2011-08-24
BRPI0921467B1 (pt) 2021-12-14
BRPI0921467A2 (pt) 2015-08-18
AU2009317860A1 (en) 2010-05-27
CN113957054B (zh) 2024-07-16
US11976287B2 (en) 2024-05-07
JP2012509059A (ja) 2012-04-19
BRPI0921467A8 (pt) 2018-06-26
CA2743880C (en) 2020-08-18
CA3015426C (en) 2020-01-14
US9976107B2 (en) 2018-05-22
JP6068800B2 (ja) 2017-01-25
US20240360464A1 (en) 2024-10-31
WO2010057246A1 (en) 2010-05-27
AR074364A1 (es) 2011-01-12
NO2358882T3 (es) 2017-12-23
CA3015426A1 (en) 2010-05-27
EP3260544B1 (en) 2024-12-25
JP6749978B2 (ja) 2020-09-02
US20180002633A1 (en) 2018-01-04
US10648046B2 (en) 2020-05-12
CN107858204B (zh) 2021-10-29
US20200385822A1 (en) 2020-12-10
DK2358882T3 (da) 2017-11-06
US12203087B2 (en) 2025-01-21
CA3082380A1 (en) 2010-05-27
US9994792B2 (en) 2018-06-12
US20170327767A1 (en) 2017-11-16
JP2016013126A (ja) 2016-01-28
US20180010141A1 (en) 2018-01-11
US20150018571A1 (en) 2015-01-15
US9938486B2 (en) 2018-04-10
CL2011001162A1 (es) 2011-08-19
CN102317459A (zh) 2012-01-11
JP2019022492A (ja) 2019-02-14
AU2009317860B2 (en) 2014-03-27
JP6746648B2 (ja) 2020-08-26
CN113957105A (zh) 2022-01-21
BR122021003836B1 (pt) 2022-02-01
CN107858204A (zh) 2018-03-30
CA3082380C (en) 2026-04-07
US20240150780A1 (en) 2024-05-09
EP3260544C0 (en) 2024-12-25
CN113957054A (zh) 2022-01-21
EP2358882A4 (en) 2012-05-02

Similar Documents

Publication Publication Date Title
ES2636487T3 (es) Producción de ácidos grasos poliinsaturados de cadena larga en células vegetales
US20240150781A1 (en) Enzymes and methods for producing omega-3 fatty acids
Singh et al. Metabolic engineering of new fatty acids in plants
EP1086236B1 (en) Polyunsaturated fatty acids in plants
Meesapyodsuk et al. The front-end desaturase: structure, function, evolution and biotechnological use
Vanhercke et al. Metabolic engineering of plant oils and waxes for use as industrial feedstocks
US7893320B2 (en) Method for producing multiple unsaturated fatty acids in plants
Abbadi et al. Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation
Graham et al. Rational metabolic engineering of transgenic plants for biosynthesis of omega-3 polyunsaturates
Zhou et al. Isolation and characterization of genes from the marine microalga Pavlova salina encoding three front-end desaturases involved in docosahexaenoic acid biosynthesis
ES2388490T3 (es) Desaturasas
ES2323644T3 (es) Desaturasas de acidos grasos procedentes de primula.
US20210290577A1 (en) Production of Omega-3 Long Chain Polyunsaturated Fatty Acids
Chen et al. Expression of Δ6, Δ5 desaturase and GLELO elongase genes from Mortierella alpina for production of arachidonic acid in soybean [Glycine max (L.) Merrill] seeds
CN101146911A (zh) 用于在转基因生物中产生具有至少四个双键的多不饱和c20-和c22-脂肪酸的方法
ES2550623T3 (es) Cártamo con ácido gamma linolénico elevado
Kang et al. Coexpression of elo-like enzyme and Δ5, Δ4-desaturases derived from Thraustochytrium aureum ATCC 34304 and the production of DHA and DPA in Pichia pastoris
Li et al. Heterologous reconstitution the polyunsaturated fatty acid biosynthetic pathway of Phaeodactylum tricornutum in Arabidopsis thaliana
Naim Enhanced metabolic engineering of lipid biosynthesis in leaves and seeds with the use of viral silencing-suppressor proteins