ES2684856R1 - Método para producir una matriz de tejido descelularizado - Google Patents

Método para producir una matriz de tejido descelularizado Download PDF

Info

Publication number
ES2684856R1
ES2684856R1 ES201890038A ES201890038A ES2684856R1 ES 2684856 R1 ES2684856 R1 ES 2684856R1 ES 201890038 A ES201890038 A ES 201890038A ES 201890038 A ES201890038 A ES 201890038A ES 2684856 R1 ES2684856 R1 ES 2684856R1
Authority
ES
Spain
Prior art keywords
adipose tissue
tissue
decellularized
decelularized
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES201890038A
Other languages
English (en)
Other versions
ES2684856B1 (es
ES2684856A2 (es
Inventor
Iratxe Madarieta Pardo
Nerea GARCÍA URQUIA
Rubén FERNANDEZ GARCÍA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fundacion Tecnalia Research and Innovation
Original Assignee
Fundacion Tecnalia Research and Innovation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fundacion Tecnalia Research and Innovation filed Critical Fundacion Tecnalia Research and Innovation
Publication of ES2684856A2 publication Critical patent/ES2684856A2/es
Publication of ES2684856R1 publication Critical patent/ES2684856R1/es
Application granted granted Critical
Publication of ES2684856B1 publication Critical patent/ES2684856B1/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/35—Fat tissue; Adipocytes; Stromal cells; Connective tissues
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P41/00—Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Epidemiology (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Zoology (AREA)
  • Dermatology (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Surgery (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Abstract

Método para la producción de una matriz de tejido descelularizado.#Se proporciona un método de descelularización de tejido adiposo que comprende las etapas de: a) laminar el tejido adiposo; b) tratar el tejido adiposo resultante de la etapa a) con una lipoproteína lipasa a 32-42 grados Celsius y a una concentración de 10-55 u/100 mg; y c) tratar el tejido adiposo resultante de la etapa b) con una nucleasa, a 32-42 grados Celsius y a una concentración de 709-1433 u/mg; durante un periodo de tiempo necesario para que el tejido adiposo tenga un contenido de ADN total igual o inferior a 50 ng/mg.#También se proporciona el tejido adiposo descelularizado obtenible por el método, productos adicionales, tales como revestimientos y armazones que comprenden el tejido descelularizado y sus usos en ingeniería tisular y terapia regenerativa.
ES201890038A 2015-12-30 2016-12-29 Metodo para producir una matriz de tejido descelularizado Active ES2684856B1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15203113 2015-12-30
EP15203113 2015-12-30
PCT/EP2016/082849 WO2017114902A1 (en) 2015-12-30 2016-12-29 Method for producing a decellularized tissue matrix

Publications (3)

Publication Number Publication Date
ES2684856A2 ES2684856A2 (es) 2018-10-04
ES2684856R1 true ES2684856R1 (es) 2018-10-09
ES2684856B1 ES2684856B1 (es) 2019-07-31

Family

ID=55168107

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201890038A Active ES2684856B1 (es) 2015-12-30 2016-12-29 Metodo para producir una matriz de tejido descelularizado

Country Status (2)

Country Link
ES (1) ES2684856B1 (es)
WO (1) WO2017114902A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108079375B (zh) 2018-02-08 2021-01-15 北京桀亚莱福生物技术有限责任公司 一种内置生物套及其制备方法与应用
CN118846235A (zh) * 2019-12-30 2024-10-29 广东泓志生物科技有限公司 大网膜脱细胞基质材料的制备方法及软骨组织的构建方法
CN114796614B (zh) * 2021-09-13 2024-02-06 暨南大学 一种人脂肪组织脱细胞外基质及其制备与应用
EP4687927A2 (en) * 2023-03-31 2026-02-11 Sinuse Corp. Allogeneic and xenogeneic subcutaneous adipose tissue and methods for preserving same
EP4704933A1 (en) 2023-05-03 2026-03-11 Volumina Medical SA Devices and methods for preparing and conditioning biological assemblies for minimally invasive delivery for tissue engineering, cell therapies and other therapeutic applications
CN116850347B (zh) * 2023-09-01 2025-01-07 北京药有为医药科技有限公司 一种可注射型脂肪脱细胞外基质复合材料的水凝胶
CN118370871B (zh) * 2024-06-24 2024-09-06 北京帝康医药投资管理有限公司 一种脱细胞生物网膜制备方法及由其制得的脱细胞生物网膜
CN118641745B (zh) * 2024-08-13 2024-10-22 浙江大学 一种基于厚切技术的全组织三维结构脱细胞基质制备方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996032905A1 (en) * 1995-04-19 1996-10-24 St. Jude Medical, Inc. Matrix substrate for a viable body tissue-derived prosthesis and method for making the same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996032905A1 (en) * 1995-04-19 1996-10-24 St. Jude Medical, Inc. Matrix substrate for a viable body tissue-derived prosthesis and method for making the same

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
Adam Young D et al. INJECTABLE HYDROGEL SCAFFOLD FROM DECELLULARIZED HUMAN LIPOASPIRATE. Acta Biomaterialia, 20100924 ELSEVIER, AMSTERDAM, NL. Kilian Kristopher; Moghe Prabhas, 24/09/2010, Vol. 7, Nº 3, Páginas 1040 - 1049 ISSN 1742-7061, (DOI: doi:10.1016/j.actbio.2010.09.035) página 1041, columna izquierda, tercer párrafo; apartado 2.4; página 1048, párrafos primero-tercero, resumen. *
Bryan N Brown et al. COMPARISON OF THREE METHODS FOR THE DERIVATION OF A BIOLOGIC SCAFFOLD COMPOSED OF ADIPOSE TISSUE EXTRACELLULAR MATRIX. Tissue Engineering Part C: Methods, 20110401 Mary Ann Liebert, Inc. , 01/04/2011, Vol. 17, Nº 4, Páginas 411 - 421 ISSN 1937-3384, (DOI: doi:10.1089/ten.tec.2010.0342) página 413, columna izquierda. *
Flynn L E. THE USE OF DECELLULARIZED ADIPOSE TISSUE TO PROVIDE AN INDUCTIVE MICROENVIRONMENT FOR THE ADIPOGENIC DIFFERENTIATION OF HUMAN ADIPOSE-DERIVED STEM CELLS. Biomaterials, 20100601 Elsevier. , 01/06/2010, Vol. 31, Nº 17, Páginas 4715 - 4724 ISSN 0142-9612, (DOI: doi:10.1016/j.biomaterials.2010.02.046) pagina 4716, columna derecha, apartado 2.3. *
Guoping chen et al. DECELLULARIZATION TECHNIQUES FOR TISSUE ENGINEERING. 12.03.2015, XP055274973, DOI: 10.1002/9780470027318.a9472. Recuperado de Internet URL: .[ Recuperado el 26.09.2018], página 4, columna izquierda, tabla 1. *
Porzionato A et al. DECELLULARIZED OMENTUM AS NOVEL BIOLOGIC SCAFFOLD FOR RECONSTRUCTIVE SURGERY AND REGENERATIVE MEDICINE. European Journal of Histochemistry: EJH Luigi Ponzio e Figlio Editori, Italy. , 30/11/0002, Vol. 57, Nº 1, Páginas 4 ISSN 1121-760X, (DOI: doi:10.4081/ejh.2013.e4) página 24, columna izquierda y derecha, último párrafo; página 25, columna izquierda; página 28, columna izquierda. *
Thomas W Gilbert. STRATEGIES FOR TISSUE AND ORGAN DECELLULARIZATION. Journal of Cellular Biochemistry, 20120701 A.R. Liss. , 01/07/2012, Vol. 113, Nº 7, Páginas 2217 - 2222 ISSN 0730-2312, (DOI: doi:10.1002/jcb.24130) página 2218, columna izquierda, párrafo 2; página 2219, columna derecho, párrafo 2-página 2220, columna izquierda, párrafo 1. *

Also Published As

Publication number Publication date
WO2017114902A1 (en) 2017-07-06
ES2684856B1 (es) 2019-07-31
ES2684856A2 (es) 2018-10-04

Similar Documents

Publication Publication Date Title
ES2684856R1 (es) Método para producir una matriz de tejido descelularizado
PE20150223A1 (es) Anticuerpos antagonistas mejorados contra factor-8 de crecimiento y diferenciacion y usos de los mismos
MX387753B (es) Proteínas de unión a calicreína en plasma y usos de las mismas para tratar el angioedema hereditario.
MX2018005829A (es) Composiciones para tratar el cabello.
MX2021008305A (es) Anticuerpos anti-cd40 para el uso en el tratamiento de hidradenitis supurativa.
CL2015000699A1 (es) Métodos para tratamiento de cáncer.
MX384240B (es) Bacterias probioticas recombinantes.
HK1252568A1 (zh) 一种治疗方法
EP4282479A3 (en) Use of pridopidine to treat depression or anxiety
EP3156081A4 (en) Cell structure for use in treatment of brain injury, method for producing same, and therapeutic agent for brain injury
CL2017001960A1 (es) Métodos de tratamiento de enfermedades inflamatorias
CL2018001742A1 (es) Compuestos indolinones y sus usos en el tratamiento de enfermedades fibroticas.
ZA202211665B (en) Ammonia oxidizing bacteria for treatment of acne
MX2014011855A (es) Sistemas y metodos para tratar una respuesta farmacodinamica adversa inducida por opioides.
BR112016030758B8 (pt) Uso de células estromais multipotentes derivadas de tecido adiposo mesenquimal
SG10201804034QA (en) Methods for treating hypotension
LT2859092T (lt) Terapinė vakcina, skirta vaikų 1 tipo diabeto gydymui, ląstelių sorterio taikymas ir treg ląstelių dauginimo būdas, siekiant gaminti terapinę vakciną 1 tipo diabeto gydymui
MX382044B (es) Oxabicicloheptanos y oxabicicloheptenos para el tratamiento de trastornos depresivos y de estrés.
EA201791455A1 (ru) Способ лечения
BR112017011513A2 (pt) tratamento de condições oculares usando células progenitoras
BR112017014552A2 (pt) ?análogo de calcitonina, e, método para produzir uma diminuição nos triglicerídeos no fígado/redução de acúmulo de gordura no fígado?
TW201613569A (en) Composition consisting of kukoamine A and/or kukoamine B and method of using thereof
MX2015012513A (es) Reactor de esterilizacion y metodo.
MX2019004804A (es) Tratamiento para el prurigo nodula.
MX2018011379A (es) Tratamiento del prurito uremico.

Legal Events

Date Code Title Description
BA2A Patent application published

Ref document number: 2684856

Country of ref document: ES

Kind code of ref document: A2

Effective date: 20181004

EC2A Search report published

Ref document number: 2684856

Country of ref document: ES

Kind code of ref document: R1

Effective date: 20181002

FG2A Definitive protection

Ref document number: 2684856

Country of ref document: ES

Kind code of ref document: B1

Effective date: 20190731