ES2722930T3 - Procedimiento de aislamiento para células madre pluripotentes derivadas de sangre umbilical que expresan ZNF281 - Google Patents

Procedimiento de aislamiento para células madre pluripotentes derivadas de sangre umbilical que expresan ZNF281 Download PDF

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Publication number
ES2722930T3
ES2722930T3 ES10753642T ES10753642T ES2722930T3 ES 2722930 T3 ES2722930 T3 ES 2722930T3 ES 10753642 T ES10753642 T ES 10753642T ES 10753642 T ES10753642 T ES 10753642T ES 2722930 T3 ES2722930 T3 ES 2722930T3
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stem cells
cells derived
pluripotent stem
isolation procedure
umbilical blood
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Kyung Sun Kang
Kyoung Hwan Roh
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SNU R&DB Foundation
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SNU R&DB Foundation
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
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  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
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  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Physical Education & Sports Medicine (AREA)
  • Neurosurgery (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Un procedimiento para aislar células madre pluripotentes o multipotentes derivadas de la sangre de cordón umbilical que expresan ZNF281, que comprende los pasos de (a) cultivar células mononucleares aisladas de la sangre de cordón umbilical en un recipiente de cultivo cubierto con fibronectina y que contiene un medio EGM-2™ o SNU-1, como se define en la Tabla 1, con suero bovino fetal (FBS), bFGF, ácido ascórbico, EGF, hidrocortisona, IGF-I, VEGF y heparina; y (b) recolectar las células madre cultivadas del cultivo, en el que la célula madre está en un estado no diferenciado.
ES10753642T 2009-03-20 2010-03-03 Procedimiento de aislamiento para células madre pluripotentes derivadas de sangre umbilical que expresan ZNF281 Active ES2722930T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090023821A KR100950195B1 (ko) 2009-03-20 2009-03-20 Ｚｎｆ281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법
PCT/KR2010/001338 WO2010107192A2 (ko) 2009-03-20 2010-03-03 Ｚｎｆ281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법

Publications (1)

Publication Number Publication Date
ES2722930T3 true ES2722930T3 (es) 2019-08-20

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ES10753642T Active ES2722930T3 (es) 2009-03-20 2010-03-03 Procedimiento de aislamiento para células madre pluripotentes derivadas de sangre umbilical que expresan ZNF281

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Country Link
US (1) US10584312B2 (es)
EP (1) EP2410046B1 (es)
JP (2) JP5995720B2 (es)
KR (1) KR100950195B1 (es)
CN (1) CN102395673B (es)
AU (1) AU2010225612B2 (es)
ES (1) ES2722930T3 (es)
MY (1) MY160164A (es)
PL (1) PL2410046T3 (es)
RU (1) RU2511417C2 (es)
WO (2) WO2010107159A1 (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100950195B1 (ko) 2009-03-20 2010-03-29 서울대학교산학협력단 Ｚｎｆ281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법
US9290739B2 (en) 2010-09-08 2016-03-22 Kang Stem Biotech Co., Ltd. Equine amniotic fluid-derived multipotent stem cells and a method for producing the same
US8940294B2 (en) 2012-03-02 2015-01-27 Tissuetech, Inc. Methods of isolating and culturing stem cells
CN103396995B (zh) * 2013-07-16 2015-09-23 广州赛哲生物科技有限公司 一种筛选乳腺癌干细胞的三维培养方法
DK3064572T3 (da) * 2013-11-01 2020-05-04 Bbhc Co Ltd Fremgangsmåde til fremstilling af inducerede pluripotente stamceller fra mesenchymale stamceller og inducerede pluripotente stamceller produceret ved fremgangsmåden
KR20180027568A (ko) * 2015-07-14 2018-03-14 메디뮨 엘엘씨 암을 치료하기 위한 조성물 및 방법
US10384207B2 (en) 2015-07-21 2019-08-20 Neuro Probe Incorporated Assay apparatus and methods
US20210355437A1 (en) 2015-09-02 2021-11-18 Restem Llc Factor rich product from umbilical cord mesenchymal stem cells
CN105087494A (zh) * 2015-09-11 2015-11-25 中国人民解放军第四军医大学 一种乳腺癌干细胞的培养方法
EP3385368B1 (en) * 2015-12-03 2023-03-01 Takara Bio Inc. Method for producing mesenchymal stem cells
JP6912789B2 (ja) * 2016-03-16 2021-08-04 株式会社日本触媒 神経幹細胞の培養方法、およびニューロスフェロイドの形成方法
AU2017307639A1 (en) * 2016-08-01 2019-03-21 Embryogenesis Pty Ltd Skin care formulation
ES3042057T3 (en) 2016-08-26 2025-11-18 Restem Llc Composition and methods of using umbilical cord lining stem cells
KR101798217B1 (ko) 2016-10-21 2017-11-15 차의과학대학교 산학협력단 만능줄기세포로부터 유래된 중간엽줄기세포를 선별하는 방법
RU2645255C1 (ru) * 2016-12-16 2018-02-19 Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр акушерства, гинекологии и перинатологии имени академика В.И.Кулакова" Министерства здравоохранения Российской Федерации Способ получения биобезопасной культуры мезенхимальных стволовых клеток из ворсин хориона человека
CN109666630A (zh) * 2017-10-17 2019-04-23 澳门大学 多能干细胞分化为间充质干细胞的方法及其培养基和应用
CN109112099A (zh) * 2018-08-30 2019-01-01 丰泽康生物医药（深圳）有限公司 一种提高单核细胞向多潜能细胞转化的无血清培养基
CN111424014B (zh) * 2019-11-22 2022-03-29 上海交通大学医学院附属第九人民医院 人颈静脉球副神经节瘤永生化细胞株培养基
TW202208613A (zh) * 2020-05-05 2022-03-01 美商加速生物科學有限公司 非永生的多潛能幹細胞
CN112430626A (zh) * 2020-11-27 2021-03-02 成都康景生物科技有限公司 一种基因修饰的脐间充质干细胞、制备方法及应用
CN116848232A (zh) * 2020-12-04 2023-10-03 株式会社普里莫里斯治疗 高表达gdf-3的脐带血干细胞分离和培养方法及gdf-3的用途
US11674090B1 (en) 2021-11-30 2023-06-13 Honeywell International Inc. Energy optimization in fluid catalytic cracking and dehydrogenation units
CN118891359A (zh) 2022-02-25 2024-11-01 康干细胞生物科技有限公司 使用无血清培养基的干细胞培养方法
WO2023163560A1 (ko) 2022-02-25 2023-08-31 주식회사 강스템바이오텍 마이크로캐리어를 이용한 줄기세포 배양 방법

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA01005564A (es) * 1998-12-04 2003-07-14 Naval Medical Res Ct Celulas del endotelio cerebral humano en medio de crecimiento y metodo para expansion de celulas de tallo de medula osea cd34+cd38-nativas.
US6610535B1 (en) 2000-02-10 2003-08-26 Es Cell International Pte Ltd. Progenitor cells and methods and uses related thereto
US20040005559A1 (en) * 2000-07-24 2004-01-08 Loring Jeanne F. Markers of neuronal differentiation and morphogenesis
CA2438501C (en) * 2001-02-14 2014-09-16 Leo T. Furcht Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof
US7736892B2 (en) * 2002-02-25 2010-06-15 Kansas State University Research Foundation Cultures, products and methods using umbilical cord matrix cells
TWI288779B (en) * 2002-03-28 2007-10-21 Blasticon Biotech Forschung Dedifferentiated, programmable stem cells of monocytic origin, and their production and use
WO2004033396A2 (en) * 2002-10-08 2004-04-22 American National Red Cross Method for enriching adherent monocyte populations
US20030224411A1 (en) * 2003-03-13 2003-12-04 Stanton Lawrence W. Genes that are up- or down-regulated during differentiation of human embryonic stem cells
JPWO2005056778A1 (ja) 2003-12-11 2007-07-12 独立行政法人理化学研究所 造血幹細胞の分化抑制又は増殖方法
WO2006012404A2 (en) * 2004-07-20 2006-02-02 Case Western Reserve University Novel cell populations and uses thereof
ES2621847T3 (es) * 2004-12-23 2017-07-05 DePuy Synthes Products, Inc. Células posparto derivadas de tejido de cordón umbilical, y métodos de elaboración y uso de las mismas
KR100697326B1 (ko) * 2005-12-02 2007-03-20 재단법인서울대학교산학협력재단 Ｏｃｔ４ 발현능을 가지는 제대혈 유래 다분화능 성체줄기세포 및 그의 제조방법
WO2008110570A1 (en) 2007-03-13 2008-09-18 Medizinische Universität Graz Method to study proliferation of endothelial progenitor cells and the potential influence of compounds on their proliferation behaviour
RU2343928C1 (ru) * 2007-04-03 2009-01-20 Открытое акционерное общество "Институт стволовых клеток человека" Способ получения ядросодержащих клеток из пуповинной крови
WO2008149129A1 (en) 2007-06-08 2008-12-11 Nova Thera Limited Cell expansion
KR100950195B1 (ko) 2009-03-20 2010-03-29 서울대학교산학협력단 Ｚｎｆ281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법

Also Published As

Publication number Publication date
US10584312B2 (en) 2020-03-10
KR100950195B1 (ko) 2010-03-29
WO2010107192A3 (ko) 2011-02-24
WO2010107192A2 (ko) 2010-09-23
MY160164A (en) 2017-02-28
JP5995720B2 (ja) 2016-09-21
EP2410046A2 (en) 2012-01-25
RU2011142170A (ru) 2013-04-27
WO2010107159A1 (ko) 2010-09-23
JP2015091254A (ja) 2015-05-14
AU2010225612B2 (en) 2014-06-26
EP2410046A4 (en) 2013-02-27
JP6038962B2 (ja) 2016-12-07
CN102395673B (zh) 2015-07-22
AU2010225612A1 (en) 2011-11-10
US20120021509A1 (en) 2012-01-26
CN102395673A (zh) 2012-03-28
RU2511417C2 (ru) 2014-04-10
EP2410046B1 (en) 2019-01-23
JP2012520671A (ja) 2012-09-10
PL2410046T3 (pl) 2019-10-31

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