ES8305934A1 - "metodo para la determinacion de las proteinas glicosiladas presentes en fluidos biologicos". - Google Patents
"metodo para la determinacion de las proteinas glicosiladas presentes en fluidos biologicos".Info
- Publication number
- ES8305934A1 ES8305934A1 ES514387A ES514387A ES8305934A1 ES 8305934 A1 ES8305934 A1 ES 8305934A1 ES 514387 A ES514387 A ES 514387A ES 514387 A ES514387 A ES 514387A ES 8305934 A1 ES8305934 A1 ES 8305934A1
- Authority
- ES
- Spain
- Prior art keywords
- biological fluids
- glycosylated proteins
- kit
- acid
- determining
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000013060 biological fluid Substances 0.000 title abstract 2
- 102000035122 glycosylated proteins Human genes 0.000 title abstract 2
- 108091005608 glycosylated proteins Proteins 0.000 title abstract 2
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 abstract 3
- 150000001720 carbohydrates Chemical class 0.000 abstract 2
- 235000014633 carbohydrates Nutrition 0.000 abstract 2
- 239000007791 liquid phase Substances 0.000 abstract 2
- RVBUGGBMJDPOST-UHFFFAOYSA-N 2-thiobarbituric acid Chemical compound O=C1CC(=O)NC(=S)N1 RVBUGGBMJDPOST-UHFFFAOYSA-N 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000002452 interceptive effect Effects 0.000 abstract 1
- 235000006408 oxalic acid Nutrition 0.000 abstract 1
- 239000007790 solid phase Substances 0.000 abstract 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 abstract 1
- 229960004319 trichloroacetic acid Drugs 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/723—Glycosylated haemoglobin
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
METODO PARA LA DETERMINACION DE LAS PROTEINAS GLICOSILADAS PRESENTES EN FLUIDOS BIOLOGICOS. COMPRENDE LAS SIGUIENTES OPERACIONES: PRIMERA, SE SOMETE LA MUESTRA EN EXAMEN A UN TRATAMIENTO EN AMBIENTE ACIDO Y A UNA TEMPERATURA SUPERIOR A 80 GRADOS, PRECEDIDO AL MENOS DE UNA PREINCUBACION UNIDA A LA ELIMINACION DE LOS CARBOHIDRATOS Y OTRAS SUSTANCIAS INTERFERENTES; SEGUNDA, SE REALIZA SUCESIVAMENTE LA PRECIPITACION DE LAS PROTEINAS, LA SEPARACION DE LA FASE LIQUIDA, LA ADICION A DICHA FASE LIQUIDA DE UN REACTIVO REVELADOR DE CARBOHIDRATOS O DE SUS DERIVADOS, Y LA ADICION DE AGUA O DE UNA SOLUCION ADECUADA A UNA PARTE ALICUOTA DE LA FASE LIQUIDA; Y POR ULTIMO, SE PROCEDE A LA LECTURA FINAL DE LAS DENSIDADES OPTICAS DE LA MUESTRA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT8122582A IT1167460B (it) | 1981-06-26 | 1981-06-26 | Dosaggio delle proteine glicosilate in fluidi biologici e mezzi adatti allo scopo |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES514387A0 ES514387A0 (es) | 1983-05-01 |
| ES8305934A1 true ES8305934A1 (es) | 1983-05-01 |
Family
ID=11198083
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES514387A Expired ES8305934A1 (es) | 1981-06-26 | 1982-06-23 | "metodo para la determinacion de las proteinas glicosiladas presentes en fluidos biologicos". |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS5821167A (es) |
| DE (1) | DE3223837A1 (es) |
| ES (1) | ES8305934A1 (es) |
| FR (1) | FR2508646A1 (es) |
| GB (1) | GB2101740A (es) |
| IT (1) | IT1167460B (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU186976B (en) * | 1982-10-01 | 1985-10-28 | Reanal Finomvegyszergyar | Process for determation of glucose containt of glucolized in non-ensimatic way proteins and reagent lutes for this process |
| US5002893A (en) * | 1985-09-19 | 1991-03-26 | Isolab, Inc. | Single color reading method for determining fructosamine |
| JPS62215398A (ja) * | 1986-01-06 | 1987-09-22 | アイソラブ，インク． | 医療診断用指示要素として羊水中のホスフアチジルグリセロ−ルの量を測定する方法とそのキツト |
| US4876188A (en) * | 1986-11-18 | 1989-10-24 | Scripps Clinic And Research Foundation | Novel immunochemical method for assaying stable glycosylated hemoglobin |
| GB9024771D0 (en) * | 1990-11-14 | 1991-01-02 | Axis Research | Assay |
| US5571723A (en) * | 1991-02-07 | 1996-11-05 | Evans; Cody A. | Method of testing for diabetes that reduces the effect of interfering substances |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4268270A (en) * | 1979-04-30 | 1981-05-19 | Children's Hospital Medical Center | Glycosylated hemoglobin measurement |
| CA1146769A (en) * | 1980-02-04 | 1983-05-24 | Beat E. Glatthaar | Process for the determination of diabetes |
-
1981
- 1981-06-26 IT IT8122582A patent/IT1167460B/it active
-
1982
- 1982-06-21 GB GB08217960A patent/GB2101740A/en not_active Withdrawn
- 1982-06-23 ES ES514387A patent/ES8305934A1/es not_active Expired
- 1982-06-25 JP JP57109652A patent/JPS5821167A/ja active Pending
- 1982-06-25 FR FR8211192A patent/FR2508646A1/fr not_active Withdrawn
- 1982-06-25 DE DE19823223837 patent/DE3223837A1/de not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| ES514387A0 (es) | 1983-05-01 |
| IT8122582A0 (it) | 1981-06-26 |
| FR2508646A1 (fr) | 1982-12-31 |
| IT1167460B (it) | 1987-05-13 |
| DE3223837A1 (de) | 1983-01-13 |
| GB2101740A (en) | 1983-01-19 |
| JPS5821167A (ja) | 1983-02-07 |
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