ES8502548A1 - Un metodo para determinar ligandos en una muestra. - Google Patents

Un metodo para determinar ligandos en una muestra.

Info

Publication number
ES8502548A1
ES8502548A1 ES527083A ES527083A ES8502548A1 ES 8502548 A1 ES8502548 A1 ES 8502548A1 ES 527083 A ES527083 A ES 527083A ES 527083 A ES527083 A ES 527083A ES 8502548 A1 ES8502548 A1 ES 8502548A1
Authority
ES
Spain
Prior art keywords
reagents
disclosure
relates
carboxyfluoresceins
substituted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES527083A
Other languages
English (en)
Other versions
ES527083A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of ES8502548A1 publication Critical patent/ES8502548A1/es
Publication of ES527083A0 publication Critical patent/ES527083A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/78—Ring systems having three or more relevant rings
    • C07D311/80—Dibenzopyrans; Hydrogenated dibenzopyrans
    • C07D311/82—Xanthenes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Endocrinology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Pyrane Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

METODO PARA DETERMINAR LIGANDOS EN UNA MUESTRA, EN PARTICULAR EN FLUIDOS BIOLOGICOS TALES COMO SUERO, PLASMA, FLUIDO ESPINAL, FLUIDO AMNIOTICO Y ORINA.COMPRENDE LAS SIGUIENTES OPERACIONES: PRIMERA, SE SOMETE A UNA REACCION COMPETITIVA LA MUESTRA CON UN TRAZADOR DE FORMULA (I) O CON SUS SALES FISIOLOGICAMENTE ACEPTABLES Y CON UN ANTICUERPO CAPAZ DE RECONOCER ESPECIFICAMENTE EL LIGANDO Y EL TRAZADOR, PARA OBTENER LOS CORRESPONDIENTES COMPLEJOS LIGANDO-ANTICUERPO Y TRAZADOR-ANTICUERPO; SEGUNDA, SE EXCITA LA MEZCLA DE REACCION CON LUZ POLARIZADA; Y POR ULTIMO, SE MIDE LA POLARIZACION DE LA FLUORESCENCIA EMITIDA PARA DETERMINAR LA CONCENTRACION DE LIGANDO PRESENTE EN DICHA MUESTRA.
ES527083A 1982-11-08 1983-11-07 Un metodo para determinar ligandos en una muestra. Granted ES527083A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/440,067 US4476229A (en) 1982-11-08 1982-11-08 Substituted carboxyfluoresceins

Publications (2)

Publication Number Publication Date
ES8502548A1 true ES8502548A1 (es) 1985-01-01
ES527083A0 ES527083A0 (es) 1985-01-01

Family

ID=23747282

Family Applications (1)

Application Number Title Priority Date Filing Date
ES527083A Granted ES527083A0 (es) 1982-11-08 1983-11-07 Un metodo para determinar ligandos en una muestra.

Country Status (6)

Country Link
US (1) US4476229A (es)
EP (1) EP0108399B1 (es)
JP (1) JPS59101480A (es)
CA (1) CA1195996A (es)
DE (1) DE3373842D1 (es)
ES (1) ES527083A0 (es)

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Also Published As

Publication number Publication date
CA1195996A (en) 1985-10-29
ES527083A0 (es) 1985-01-01
JPS59101480A (ja) 1984-06-12
DE3373842D1 (en) 1987-10-29
EP0108399A3 (en) 1984-06-13
JPH0475465B2 (es) 1992-11-30
EP0108399A2 (en) 1984-05-16
EP0108399B1 (en) 1987-09-23
US4476229A (en) 1984-10-09

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