ES8708162A1 - Procedimiento de dosificar enzimas por bioluminiscencia y perfeccionamientos en los soportes para su realizacion - Google Patents
Procedimiento de dosificar enzimas por bioluminiscencia y perfeccionamientos en los soportes para su realizacionInfo
- Publication number
- ES8708162A1 ES8708162A1 ES556943A ES556943A ES8708162A1 ES 8708162 A1 ES8708162 A1 ES 8708162A1 ES 556943 A ES556943 A ES 556943A ES 556943 A ES556943 A ES 556943A ES 8708162 A1 ES8708162 A1 ES 8708162A1
- Authority
- ES
- Spain
- Prior art keywords
- substrates
- enzymes
- dosing
- bioluminescent
- inhibitors
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 5
- 102000004190 Enzymes Human genes 0.000 title abstract 5
- 239000000758 substrate Substances 0.000 title abstract 5
- 230000002255 enzymatic effect Effects 0.000 title abstract 4
- 239000003112 inhibitor Substances 0.000 title abstract 3
- 108060001084 Luciferase Proteins 0.000 abstract 2
- 239000005089 Luciferase Substances 0.000 abstract 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 2
- 238000001179 sorption measurement Methods 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 abstract 1
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 abstract 1
- 108020005199 Dehydrogenases Proteins 0.000 abstract 1
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 abstract 1
- 102000001253 Protein Kinase Human genes 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 abstract 1
- 229920002223 polystyrene Polymers 0.000 abstract 1
- 108060006633 protein kinase Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
MODIFICACIONES EN EL PROCEDIMIENTO DE DOSIFICAR ENZIMAS POR BIOLUMINISCENCIA. CONSISTENTES EN QUE: SE UTILIZA UN SOPORTE TRANSPARENTE A BASE DE UN MATERIAL PLASTICO SINTETICO TRATADO PREVIAMENTE CON UNA SUSTANCIA HIDROFILA CARGADA, PARA LA FIJACION POR ABSORCION DE UN SISTEMA ENZIMATICO QUE CONTIENE POR LO MENOS UNA LUCIFERASA; LA SUSTANCIA HIDROFILA CONTIENE UN POLIMERO DE ACIDOS AMINADOS COMO LA POLIARGININA Y POLIHISTIDINA SOLA Y/O UNO DE SUS COPOLIMEROS; EL SISTEMA ENZIMATICO CONTIENE LUCIFERASA Y NADH:FMN OXIDO-REDUCTASA PARA EL DOSIFICADO DE LA NADH O DE UNA DESHIDROGENASA CON NADH.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BE85215257A BE902745A (fr) | 1985-06-26 | 1985-06-26 | Methode de dosage par bioluminescence a l'aide d'enzymes immobilisees. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES556943A0 ES556943A0 (es) | 1987-10-01 |
| ES8708162A1 true ES8708162A1 (es) | 1987-10-01 |
Family
ID=3843907
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES556943A Expired ES8708162A1 (es) | 1985-06-26 | 1986-06-25 | Procedimiento de dosificar enzimas por bioluminiscencia y perfeccionamientos en los soportes para su realizacion |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0229085B1 (es) |
| JP (1) | JPS63500631A (es) |
| AU (1) | AU592096B2 (es) |
| DE (1) | DE3671646D1 (es) |
| ES (1) | ES8708162A1 (es) |
| WO (1) | WO1987000198A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2636970B1 (fr) * | 1988-09-26 | 1991-11-29 | Inst Nat Sante Rech Med | Procede de detection et de dosage d'une sequence specifique d'adn ou d'arn, reactif et necessaire pour sa mise en oeuvre |
| JP3236199B2 (ja) * | 1995-08-25 | 2001-12-10 | 日本電気株式会社 | 平面光導波路型バイオケミカルセンサ |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2165832A1 (en) * | 1971-04-20 | 1973-08-10 | Research Corp | Enzymatically-active membranes - immobilisation of enzymes by complex formation on protein membranes |
| US4412001A (en) * | 1981-01-30 | 1983-10-25 | Board Of Trustees Of The University Of Illinois | Isolation of bacterial luciferase |
| AU2582984A (en) * | 1983-03-17 | 1984-09-20 | Minnesota Mining And Manufacturing Company | Fluorometric assay of total ige levels |
-
1986
- 1986-06-19 DE DE8686903170T patent/DE3671646D1/de not_active Expired - Lifetime
- 1986-06-19 JP JP61503361A patent/JPS63500631A/ja active Pending
- 1986-06-19 EP EP86903170A patent/EP0229085B1/fr not_active Expired - Lifetime
- 1986-06-19 WO PCT/BE1986/000020 patent/WO1987000198A1/fr not_active Ceased
- 1986-06-19 AU AU59674/86A patent/AU592096B2/en not_active Ceased
- 1986-06-25 ES ES556943A patent/ES8708162A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| AU592096B2 (en) | 1990-01-04 |
| DE3671646D1 (de) | 1990-07-05 |
| AU5967486A (en) | 1987-01-30 |
| EP0229085A1 (fr) | 1987-07-22 |
| EP0229085B1 (fr) | 1990-05-30 |
| JPS63500631A (ja) | 1988-03-10 |
| ES556943A0 (es) | 1987-10-01 |
| WO1987000198A1 (fr) | 1987-01-15 |
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