ES8801552A1 - Un procedimiento para preparar una proteina hibrida fibrinoliticamente activa. - Google Patents

Un procedimiento para preparar una proteina hibrida fibrinoliticamente activa.

Info

Publication number
ES8801552A1
ES8801552A1 ES557677A ES557677A ES8801552A1 ES 8801552 A1 ES8801552 A1 ES 8801552A1 ES 557677 A ES557677 A ES 557677A ES 557677 A ES557677 A ES 557677A ES 8801552 A1 ES8801552 A1 ES 8801552A1
Authority
ES
Spain
Prior art keywords
fibrinolytic enzyme
modified
region
growth factor
plasminogen activator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES557677A
Other languages
English (en)
Other versions
ES557677A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beecham Group PLC
Original Assignee
Beecham Group PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB858508972A external-priority patent/GB8508972D0/en
Priority claimed from GB868604852A external-priority patent/GB8604852D0/en
Application filed by Beecham Group PLC filed Critical Beecham Group PLC
Publication of ES557677A0 publication Critical patent/ES557677A0/es
Publication of ES8801552A1 publication Critical patent/ES8801552A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE PROTEINAS HIBRIDAS. CONSISTE EN FIJAR EL ADN QUE CODIFICA LA CADENA A DE T-PA MODIFICADA EN LA REGION DEL DOMINIO DEL FACTOR DE CRECIMIENTO, AL ADN QUE CODIFICA LA CADENA B DE UNA PROTEASA ACTIVA FIBRINOLITICAMENTE, EN PRESENCIA DE UNA LIGASA; EXPRESAR EL ADN FORMADO EN UNA CELULA HUESPED RECOMBINANTE, EN UN MEDIO ACUOSO NUTRIENTE QUE CONTIENE SALES INORGANICAS Y FUENTES DE CARBONO Y NITROGENO ASIMILABLES, A UNA TEMPERATURA COMPRENDIDA ENTRE LA AMBIENTE Y 45JC; Y RECUPERAR EL PRODUCTO PROTEICO MEDIANTE SEPARACION DEL MEDIO NUTRIENTE. ESTOS COMPUESTOS TIENEN APLICACIONES FARMACOLOGICAS PARA EL TRATAMIENTO DE ENFERMEDADES TROMBOTICAS.
ES557677A 1985-04-04 1987-08-17 Un procedimiento para preparar una proteina hibrida fibrinoliticamente activa. Expired ES8801552A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB858508972A GB8508972D0 (en) 1985-04-04 1985-04-04 Compounds
GB868604852A GB8604852D0 (en) 1986-02-27 1986-02-27 Compounds

Publications (2)

Publication Number Publication Date
ES557677A0 ES557677A0 (es) 1987-12-16
ES8801552A1 true ES8801552A1 (es) 1987-12-16

Family

ID=26289098

Family Applications (5)

Application Number Title Priority Date Filing Date
ES553651A Expired ES8801374A1 (es) 1985-04-04 1986-04-02 Un procedimiento para preparar un activante de plasminogeno.
ES557676A Expired ES8802537A1 (es) 1985-04-04 1987-08-17 Un procedimiento para preparar una celula huesped con un vector de expresion correspondiente a un activante de plasminogenos de tipo tejido modificado.
ES557677A Expired ES8801552A1 (es) 1985-04-04 1987-08-17 Un procedimiento para preparar una proteina hibrida fibrinoliticamente activa.
ES557675A Expired ES8801551A1 (es) 1985-04-04 1987-08-17 Un procedimiento para preparar un vector de expresion replicable.
ES557674A Expired ES8802442A1 (es) 1985-04-04 1987-08-17 Un procedimiento para preparar un polimero de adn.

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ES553651A Expired ES8801374A1 (es) 1985-04-04 1986-04-02 Un procedimiento para preparar un activante de plasminogeno.
ES557676A Expired ES8802537A1 (es) 1985-04-04 1987-08-17 Un procedimiento para preparar una celula huesped con un vector de expresion correspondiente a un activante de plasminogenos de tipo tejido modificado.

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES557675A Expired ES8801551A1 (es) 1985-04-04 1987-08-17 Un procedimiento para preparar un vector de expresion replicable.
ES557674A Expired ES8802442A1 (es) 1985-04-04 1987-08-17 Un procedimiento para preparar un polimero de adn.

Country Status (6)

Country Link
EP (1) EP0207589A1 (es)
DK (1) DK149786A (es)
ES (5) ES8801374A1 (es)
GR (1) GR860875B (es)
NZ (1) NZ215660A (es)
PT (1) PT82326B (es)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5106741A (en) * 1985-12-20 1992-04-21 The Upjohn Company Tissue plasminogen activator (TPA) analogs
WO1987003906A1 (en) * 1985-12-20 1987-07-02 The Upjohn Company Tissue plasminogen activator (tpa) analogs
US5258298A (en) * 1986-01-31 1993-11-02 Genetics Institute, Inc. Deletion and glycosylation mutant of human tissue plasminogen activator
US5002887A (en) * 1986-01-31 1991-03-26 Genetics Institute, Inc. Truncated thrombolytic proteins
US5837518A (en) * 1986-01-31 1998-11-17 Genetics Institute, Inc. Thrombolytic proteins
DE3682891D1 (de) * 1986-02-17 1992-01-23 Stichting Centraal Lab Gewebeplasminogen-aktivator-mutant, dafuer kodierende rekombinante genetische information und verfahren zur herstellung dieser mutanten, deren verwendung und pharmazeutische zusammensetzungen.
PT84588B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
NZ219829A (en) * 1986-04-02 1990-05-28 Beecham Group Plc Tissue-type plasminogen activator modified in the region of amino acids 67-69 and pharmaceutical compositions thereof
PT84589B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
DE3643158A1 (de) * 1986-04-21 1987-11-19 Boehringer Mannheim Gmbh Gewebs-plasminogen-aktivator(tpa) - derivat und seine herstellung
EP0266032A1 (en) * 1986-08-29 1988-05-04 Beecham Group Plc Modified fibrinolytic enzyme
US5242819A (en) * 1986-12-05 1993-09-07 Ciba-Geigy Corporation DNA molecules encoding hybrid proteins of tissue plasminogen activator and urokinase
US5580559A (en) * 1986-12-05 1996-12-03 Ciba-Geigy Corporation Hybrid plasminogen activator
GB8701811D0 (en) * 1987-01-28 1987-03-04 Beecham Group Plc Compounds
IE880255L (en) * 1987-01-30 1988-07-30 American Home Prod Des-epidermal growth factor activators.
NL8701021A (nl) * 1987-04-29 1988-11-16 Stichting Centraal Lab Humane t-pa(u-pa) substitutie-mutant eiwitten, daarvoor coderend recombinant dna, getransfecteerde gastheercellen, bereiding van de mutant eiwitten, en farmaceutische preparaten.
ZA883958B (en) * 1987-06-04 1989-07-26 Zymogenetics Inc Mutant t-pa with ringle replacement
NO179874C (no) * 1987-06-04 1997-01-02 Zymogenetics Inc Fremgangsmåte for fremstilling av en t-PA-analog, DNA-sekvens som koder for t-PA-analogen, samt ekspresjonsvektor omfattende DNA-sekvensen og vertcelle omfattende denne
SE8702562L (sv) * 1987-06-18 1988-12-19 Kabigen Ab Nya fibrinolytiska enzymer
AU613942B2 (en) * 1987-06-30 1991-08-15 Genentech Inc. Improved processes for the treatment of vascular disease
JPH02504102A (ja) * 1987-06-30 1990-11-29 ジェネンテク，インコーポレイテッド 血管障害治療のための改良法
US4935237A (en) * 1988-03-21 1990-06-19 Genentech, Inc. Processes for the preparation of t-PA mutants
WO1989000191A1 (en) * 1987-07-06 1989-01-12 Genetics Institute, Inc. Novel thrombolytic proteins
GB8721023D0 (en) * 1987-09-07 1987-10-14 Lepetit Spa Modified plasminogen activators
US5100666A (en) * 1987-10-09 1992-03-31 Monsanto Company Modified tissue plasminogen activator K2K2SP
US5244676A (en) * 1987-10-09 1993-09-14 Monsanto Company Modified tissue plasminogen activator with modified glycosylation site
US5037646A (en) * 1988-04-29 1991-08-06 Genentech, Inc. Processes for the treatment of vascular disease
US5270198A (en) * 1988-05-20 1993-12-14 Genentech, Inc. DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells
US5346824A (en) * 1988-05-20 1994-09-13 Genentech, Inc. DNA encoding variants of tissue plasminogen activators and expression vectors and hosts thereof
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
DK0643772T3 (da) * 1992-06-03 1998-02-02 Genentech Inc Glycosyleringsvarianter af vævsplasminogenaktivator med forbedrede terapeutiske egenskaber
GB9614871D0 (en) 1996-07-15 1996-09-04 Smithkline Beecham Plc Compounds

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
AU572108B2 (en) * 1983-01-19 1988-05-05 Genentech Inc. Human tpa production using vectors coding for dhfr protein

Also Published As

Publication number Publication date
DK149786D0 (da) 1986-04-02
ES8801551A1 (es) 1987-12-16
AU5551486A (en) 1986-10-09
ES557677A0 (es) 1987-12-16
PT82326A (en) 1986-05-01
EP0207589A1 (en) 1987-01-07
ES8802537A1 (es) 1988-07-16
GR860875B (en) 1986-07-29
ES557675A0 (es) 1987-12-16
ES8802442A1 (es) 1988-06-01
AU596093B2 (en) 1990-04-26
DK149786A (da) 1986-10-04
ES8801374A1 (es) 1988-01-01
NZ215660A (en) 1990-03-27
ES553651A0 (es) 1988-01-01
ES557674A0 (es) 1988-06-01
PT82326B (pt) 1988-10-14
ES557676A0 (es) 1988-07-16

Similar Documents

Publication Publication Date Title
ES8801551A1 (es) Un procedimiento para preparar un vector de expresion replicable.
AU5330286A (en) Modified tissue-type plasminogen activator
AU4777785A (en) Modified tissue plasminogen activators
AU7090887A (en) Plasminogen activators modified in first kringle domain
GB2055850B (en) Thermo-stable micro-organism and proteolytic enzyme prepared therefrom
ES360055A1 (es) Un procedimiento para obtener preparados en enzimas proteo-liticas.
AU6772387A (en) Treatment of thrombosis with fibrinolytic agents and prostacyclines
AU7090687A (en) Modified tissue-type plasminogen activators
ES8703521A1 (es) Procedimiento para determinar un activador de plasminogeno
AU679512B2 (en) Bifunctional variants of urokinase with improved fibrinolytic properties and thrombin-inhibiting activity
EP0351246A3 (en) A tissue plasminogen activator analogue
AU2778889A (en) Fibrin selective two-chain plasminogen activator
AU2196688A (en) Modified plasminogen activators
EP0139447A3 (en) Urokinase zymogen and composition containing the same
AU2353688A (en) Modified tissue plasminogen activator
AU602681B2 (en) Modified plasminogen activators
ES8704542A1 (es) Un procedimiento para la preparacion de derivados enzimaticos.
IE873398L (en) New plasminogen activators.
EP0292326A3 (en) Modified enzyme
EP0277751A3 (en) Modified fibrinolytic enzyme
WO1991019793A3 (en) Hybrid plasminogen activators
BG103305A (en) Intermediate compounds for the preparation of hiv protease inhibitors and methods for the preparation of hiv protease inhibitors
IE860355L (en) Modified tissue-type plasminogen activator
HUT34950A (en) Process for the production of alpha-allene-alpha-amino acids and of therapeutic preparations containing such agents
IL111767A (en) Method for identifying improved activity tissue plasminogen activator variants with substituted amino acid residue in the protease domain

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970303