ES8802183A1 - Un procedimiento para preparar el plasmido pcr712 o pcr713 - Google Patents

Un procedimiento para preparar el plasmido pcr712 o pcr713

Info

Publication number
ES8802183A1
ES8802183A1 ES550866A ES550866A ES8802183A1 ES 8802183 A1 ES8802183 A1 ES 8802183A1 ES 550866 A ES550866 A ES 550866A ES 550866 A ES550866 A ES 550866A ES 8802183 A1 ES8802183 A1 ES 8802183A1
Authority
ES
Spain
Prior art keywords
prochymosin
cdna sequence
expression plasmids
plasmids containing
full cdna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES550866A
Other languages
English (en)
Other versions
ES550866A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of ES8802183A1 publication Critical patent/ES8802183A1/es
Publication of ES550866A0 publication Critical patent/ES550866A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cephalosporin Compounds (AREA)

Abstract

COMPRENDE LAS SIGUIENTES ETAPAS: A) DIGERIR EL PLASMIDO PCR701 (FIG. 1), CON HIND III MEDIANTE INCUBACION EN UNA SOLUCION QUE CONTIENE ADEMAS TAMPON HIND III B) INCUBAR LOS DIGERIDOS DE LA ETAPA A), EN PRESENCIA DE NUCLEASA S, EN UNA SOLUCION TAMPONADA; C) LIGAR LOS DNAS OBTENIDOS EN LA ETAPA ANTERIOR MEDIANTE INCUBACION EN UNA SOLUCION QUE CONTIENE ADEMAS DNA LIGASA T4, ATP Y TAMPON DE LIGACION. PARA CADA ETAPA SE PROPORCIONAN LOS INTERVALOS ADECUADOS DE TIEMPO Y TEMPERATURA. SE DESCRIBEN DOS TIPOS DISTINTOS DE SOLUCION TAMPONADA EN LA ETAPA B, QUE DEN LUGAR A POSIBILIDADES ALTERNATIVAS EN LA ETAPA C). DE APLICACION EN LA OBTENCION DE PROQUIMOSINA, QUE SE UTILIZA EN LA MANUFACTURA E INDUSTRIA DEL QUESO PARA LA COAGULACION DE LA LECHE.
ES550866A 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr712 o pcr713 Expired ES8802183A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP59044901A JPS60188077A (ja) 1984-03-09 1984-03-09 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド

Publications (2)

Publication Number Publication Date
ES8802183A1 true ES8802183A1 (es) 1988-04-01
ES550866A0 ES550866A0 (es) 1988-04-01

Family

ID=12704373

Family Applications (4)

Application Number Title Priority Date Filing Date
ES541121A Expired ES8705520A1 (es) 1984-03-09 1985-03-08 Un procedimiento para preparar el plasmido plr 711
ES550866A Expired ES8802183A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr712 o pcr713
ES550868A Expired ES8802181A1 (es) 1984-03-09 1986-01-14 Un procedimiento para la produccion de proquimosina
ES550867A Expired ES8704205A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr714

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES541121A Expired ES8705520A1 (es) 1984-03-09 1985-03-08 Un procedimiento para preparar el plasmido plr 711

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES550868A Expired ES8802181A1 (es) 1984-03-09 1986-01-14 Un procedimiento para la produccion de proquimosina
ES550867A Expired ES8704205A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr714

Country Status (11)

Country Link
US (1) US4757020A (es)
EP (1) EP0154350B1 (es)
JP (1) JPS60188077A (es)
AT (1) ATE74161T1 (es)
AU (1) AU594012B2 (es)
CA (1) CA1288072C (es)
DE (1) DE3585695D1 (es)
DK (1) DK107885A (es)
ES (4) ES8705520A1 (es)
IE (1) IE58820B1 (es)
IL (1) IL74537A (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3514113A1 (de) * 1985-04-19 1986-10-23 Hoechst Ag, 6230 Frankfurt Veraenderung der dna-sequenz zwischen shine-dalgarno-sequenz und startcodon des trp-operons zur steigerung der proteinexpression
IT1221765B (it) * 1986-05-07 1990-07-12 Eniricerche Spa Sequenza nucleotidica capace di indurre alti livelli di traduzione di un gene eterologo in bacillus subtilis ed escherichia coli
US5045471A (en) * 1986-12-02 1991-09-03 The Regents Of The University Of California Cloned DNA for P450scc and expression thereof
JPH05505308A (ja) * 1990-03-13 1993-08-12 ハワイ・バイオテクノロジー・グループ・インコーポレイテツド アオパンカビ発現システム
US7482148B2 (en) * 2004-03-30 2009-01-27 Sudershan Biotech Ltd. Recombinant calf-chymosin and a process for producing the same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
IE53517B1 (en) * 1981-06-17 1988-12-07 Celltech Ltd Process for the production of a polypeptide
JPS589687A (ja) * 1981-06-17 1983-01-20 セルテク　リミテッド ポリペプチドの生産方法
JPS58110600A (ja) * 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
JPS59166086A (ja) * 1983-03-09 1984-09-19 Teruhiko Beppu 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法

Also Published As

Publication number Publication date
AU594012B2 (en) 1990-03-01
ES8802181A1 (es) 1988-04-01
IL74537A0 (en) 1985-06-30
DE3585695D1 (de) 1992-04-30
US4757020A (en) 1988-07-12
AU3964085A (en) 1985-09-12
EP0154350A3 (en) 1987-04-22
IL74537A (en) 1992-03-29
DK107885D0 (da) 1985-03-08
JPS60188077A (ja) 1985-09-25
ES550868A0 (es) 1988-04-01
ES550867A0 (es) 1987-03-16
JPH0515432B2 (es) 1993-03-01
ES8704205A1 (es) 1987-03-16
IE58820B1 (en) 1993-11-17
ATE74161T1 (de) 1992-04-15
ES8705520A1 (es) 1987-05-01
EP0154350B1 (en) 1992-03-25
CA1288072C (en) 1991-08-27
ES541121A0 (es) 1987-05-01
EP0154350A2 (en) 1985-09-11
DK107885A (da) 1985-09-10
IE850606L (en) 1985-09-09
ES550866A0 (es) 1988-04-01

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20020401