FI62859C - Foerfarande och reagens foer bestaemning av heparinets biologiska aktivitet i plasma - Google Patents

Foerfarande och reagens foer bestaemning av heparinets biologiska aktivitet i plasma Download PDF

Info

Publication number: FI62859C
Authority: FI; Finland
Prior art keywords: reagent; plasma; arg; pna; heparin
Prior art date: 1978-03-23

Application number

FI790476A

Other languages

English (en)

Finnish (fi)

Other versions

FI62859B (fi

FI790476A7 (fi

Inventor

Knut Bartl

Helmut Lill

Joachim Ziegenhorn

Original Assignee

Boehringer Mannheim Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1978-03-23

Filing date

1979-02-13

Publication date

1983-03-10

1979-02-13 Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh

1979-09-24 Publication of FI790476A7 publication Critical patent/FI790476A7/fi

1982-11-30 Application granted granted Critical

1982-11-30 Publication of FI62859B publication Critical patent/FI62859B/fi

1983-03-10 Publication of FI62859C publication Critical patent/FI62859C/fi

Links

230000004071 biological effect Effects 0.000 title claims description 10
HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 34
229960002897 heparin Drugs 0.000 claims description 34
229920000669 heparin Polymers 0.000 claims description 34
238000000034 method Methods 0.000 claims description 27
108090000190 Thrombin Proteins 0.000 claims description 22
229960004072 thrombin Drugs 0.000 claims description 22
239000003153 chemical reaction reagent Substances 0.000 claims description 18
239000003593 chromogenic compound Substances 0.000 claims description 13
238000003556 assay Methods 0.000 claims description 12
108010074860 Factor Xa Proteins 0.000 claims description 10
108010039627 Aprotinin Proteins 0.000 claims description 8
108091005804 Peptidases Proteins 0.000 claims description 8
102000035195 Peptidases Human genes 0.000 claims description 8
229960004405 aprotinin Drugs 0.000 claims description 8
239000000872 buffer Substances 0.000 claims description 8
ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 claims description 8
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 7
VYLJFJGMPYBPMN-VXKWHMMOSA-N (2s)-n-[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]-1-[2-[(4-methylphenyl)sulfonylamino]acetyl]pyrrolidine-2-carboxamide Chemical compound C1=CC(C)=CC=C1S(=O)(=O)NCC(=O)N1[C@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=2C=CC(=CC=2)[N+]([O-])=O)CCC1 VYLJFJGMPYBPMN-VXKWHMMOSA-N 0.000 claims description 6
239000002202 Polyethylene glycol Substances 0.000 claims description 6
230000008033 biological extinction Effects 0.000 claims description 6
108010018472 chromozym TH Proteins 0.000 claims description 6
229920001223 polyethylene glycol Polymers 0.000 claims description 6
238000011534 incubation Methods 0.000 claims description 5
230000008569 process Effects 0.000 claims description 5
239000000758 substrate Substances 0.000 claims description 5
238000005259 measurement Methods 0.000 claims description 4
ZYECEUZMVSGTMR-LBDWYMBGSA-N (4s)-4-[[(2s,3s)-2-benzamido-3-methylpentanoyl]amino]-5-[[2-[[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-5-oxopentanoic acid Chemical compound N([C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=1C=CC(=CC=1)[N+]([O-])=O)C(=O)C1=CC=CC=C1 ZYECEUZMVSGTMR-LBDWYMBGSA-N 0.000 claims description 3
102000004411 Antithrombin III Human genes 0.000 claims description 3
108090000935 Antithrombin III Proteins 0.000 claims description 3
239000003513 alkali Substances 0.000 claims description 3
229960005348 antithrombin iii Drugs 0.000 claims description 3
125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 claims description 3
108010031969 benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide Proteins 0.000 claims description 3
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 3
108090000765 processed proteins & peptides Proteins 0.000 claims description 3
YDMBNDUHUNWWRP-VJBWXMMDSA-N (2s)-1-[(2r)-2-amino-3-phenylpropanoyl]-n-[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]piperidine-2-carboxamide Chemical compound C([C@@H](N)C(=O)N1[C@@H](CCCC1)C(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=1C=CC(=CC=1)[N+]([O-])=O)C1=CC=CC=C1 YDMBNDUHUNWWRP-VJBWXMMDSA-N 0.000 claims description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 2
108010039286 S 2238 Proteins 0.000 claims description 2
QQVNCBCBFNWLJX-KCHLEUMXSA-N n-[(2s)-1-[[(2s)-1-[[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]benzamide Chemical compound C([C@@H](C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=1C=CC(=CC=1)[N+]([O-])=O)NC(=O)C=1C=CC=CC=1)C1=CC=CC=C1 QQVNCBCBFNWLJX-KCHLEUMXSA-N 0.000 claims description 2
230000005855 radiation Effects 0.000 claims description 2
238000003756 stirring Methods 0.000 claims description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
239000000975 dye Substances 0.000 description 8
230000000694 effects Effects 0.000 description 7
RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 6
108090000790 Enzymes Proteins 0.000 description 5
102000004190 Enzymes Human genes 0.000 description 5
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 5
229940088598 enzyme Drugs 0.000 description 5
239000011780 sodium chloride Substances 0.000 description 5
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 5
208000007536 Thrombosis Diseases 0.000 description 4
238000006243 chemical reaction Methods 0.000 description 4
230000035945 sensitivity Effects 0.000 description 4
238000002560 therapeutic procedure Methods 0.000 description 4
TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 description 3
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
239000007983 Tris buffer Substances 0.000 description 3
239000008280 blood Substances 0.000 description 3
210000004369 blood Anatomy 0.000 description 3
230000008859 change Effects 0.000 description 3
239000000243 solution Substances 0.000 description 3
102000009123 Fibrin Human genes 0.000 description 2
108010073385 Fibrin Proteins 0.000 description 2
BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
230000008901 benefit Effects 0.000 description 2
230000023555 blood coagulation Effects 0.000 description 2
230000035602 clotting Effects 0.000 description 2
230000015271 coagulation Effects 0.000 description 2
238000005345 coagulation Methods 0.000 description 2
229950003499 fibrin Drugs 0.000 description 2
230000002779 inactivation Effects 0.000 description 2
238000001228 spectrum Methods 0.000 description 2
239000012085 test solution Substances 0.000 description 2
YSRMCZDHEZNRPS-HOTGVXAUSA-N (2s)-5-(diaminomethylideneamino)-2-[[(2s)-1-[2-[(4-methylphenyl)sulfonylamino]acetyl]pyrrolidine-2-carbonyl]amino]pentanoic acid Chemical compound C1=CC(C)=CC=C1S(=O)(=O)NCC(=O)N1[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)CCC1 YSRMCZDHEZNRPS-HOTGVXAUSA-N 0.000 description 1
201000005657 Antithrombin III deficiency Diseases 0.000 description 1
206010053567 Coagulopathies Diseases 0.000 description 1
108010049003 Fibrinogen Proteins 0.000 description 1
102000008946 Fibrinogen Human genes 0.000 description 1
241000234435 Lilium Species 0.000 description 1
-1 NaCl or KCl Chemical class 0.000 description 1
239000004365 Protease Substances 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
230000004913 activation Effects 0.000 description 1
239000000853 adhesive Substances 0.000 description 1
230000001070 adhesive effect Effects 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
239000004019 antithrombin Substances 0.000 description 1
230000007321 biological mechanism Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
230000000740 bleeding effect Effects 0.000 description 1
150000001805 chlorine compounds Chemical class 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
229940012952 fibrinogen Drugs 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
208000033666 hereditary antithrombin deficiency Diseases 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
239000000203 mixture Substances 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
230000002797 proteolythic effect Effects 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000001356 surgical procedure Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/81—Protease inhibitors
- G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- G01N2333/811—Serine protease (E.C. 3.4.21) inhibitors
- G01N2333/8114—Kunitz type inhibitors
- G01N2333/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
- G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
- G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
- G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
- G01N2333/96433—Serine endopeptidases (3.4.21)
- G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
- G01N2333/96444—Factor X (3.4.21.6)
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/974—Thrombin
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, Konjac gum, Locust bean gum or Guar gum
- G01N2400/40—Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Neurosurgery (AREA)
Biotechnology (AREA)
Immunology (AREA)
Analytical Chemistry (AREA)
Molecular Biology (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Hematology (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

FI790476A 1978-03-23 1979-02-13 Foerfarande och reagens foer bestaemning av heparinets biologiska aktivitet i plasma FI62859C (fi)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE2812943		1978-03-23
DE2812943A DE2812943C3 (de)	1978-03-23	1978-03-23	Verfahren und Reagens zur Bestimmung der biologischen Aktivität von Heparin im Plasma

Publications (3)

Publication Number	Publication Date
FI790476A7 FI790476A7 (fi)	1979-09-24
FI62859B FI62859B (fi)	1982-11-30
FI62859C true FI62859C (fi)	1983-03-10

Family

ID=6035376

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
FI790476A FI62859C (fi)	1978-03-23	1979-02-13	Foerfarande och reagens foer bestaemning av heparinets biologiska aktivitet i plasma

Country Status (22)

Country	Link
US (1)	US4234682A (fr)
EP (1)	EP0004271A3 (fr)
JP (1)	JPS54128796A (fr)
AR (1)	AR217503A1 (fr)
AT (1)	AT362080B (fr)
AU (1)	AU512206B2 (fr)
BE (1)	BE875014A (fr)
CA (1)	CA1111334A (fr)
CH (1)	CH642749A5 (fr)
DD (1)	DD141952A5 (fr)
DE (1)	DE2812943C3 (fr)
DK (1)	DK149475C (fr)
FI (1)	FI62859C (fr)
FR (1)	FR2420764A1 (fr)
GB (1)	GB2017298B (fr)
HU (1)	HU176068B (fr)
IE (1)	IE48067B1 (fr)
IT (1)	IT1112966B (fr)
LU (1)	LU81070A1 (fr)
NL (1)	NL7902259A (fr)
SE (1)	SE445560B (fr)
ZA (1)	ZA79622B (fr)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE2903701C2 (de) *	1979-01-31	1980-10-16	Boehringer Mannheim Gmbh, 6800 Mannheim	Kontrollreagens für die Bestimmung der Heparinaktivität
US4244865A (en) *	1979-12-03	1981-01-13	Abbott Laboratories	α hydroxy tripeptide substrates
DE3005540A1 (de) *	1980-02-14	1981-08-20	Boehringer Mannheim Gmbh, 6800 Mannheim	Verfahren und reagens zur bestimmung der biologischen aktivitaet von heparin im plasma
DE3019612A1 (de) *	1980-05-22	1981-11-26	Boehringer Mannheim Gmbh, 6800 Mannheim	Stabilisiertes thrombinpraeparat
WO1982001377A1 (fr) *	1980-10-09	1982-04-29	Lill Helmut	Procede de determination d'une bm ('binding moderately') antithrombine
US4473639A (en) *	1982-09-15	1984-09-25	Miles Laboratories, Inc.	Reagent strip test for antithrombin-III
US4543335A (en) *	1982-12-20	1985-09-24	Miles Laboratories, Inc.	Device and method for the quantitative determination of heparin in mammalian blood plasma
US4946775A (en) *	1985-09-05	1990-08-07	Yin E Thye	Composition, kit and method for assaying heparin and a method for making the composition
DE3679763D1 (de) *	1985-09-05	1991-07-18	E Thye Yin	Verfahren und zusammensetzungen zur bestimmung von heparin.
US4948724A (en) *	1985-09-05	1990-08-14	Yin E Thye	Composition, kit and method for assaying heparin and a method for making the composition
US4851336A (en) *	1985-09-05	1989-07-25	Yin E Thye	Composition, kit, and method for assaying heparinano a method for making the composition
AU6067190A (en) *	1989-07-20	1991-02-22	Analytical Control Systems, Inc.	Improved stable coagulation controls
US5939325A (en) *	1989-07-20	1999-08-17	Analytical Control Systems, Inc.	Stable whole blood coagulation controls
WO1992007954A1 (fr) *	1990-11-05	1992-05-14	Baxter Diagnostics Inc.	Procede de determination de la concentration d'anticoagulants
US5308755A (en) *	1992-06-08	1994-05-03	Research Corporation Technologies, Inc.	Method for measuring heparin
EP1325962A1 (fr) *	2001-12-17	2003-07-09	Gentium S.p.A.	Procédé pour la détermination de l'activité biologique de la defibrotide
EP1745145A4 (fr) *	2004-05-11	2008-03-26	Heptest Lab Inc	Compositions, kit et procede en une etape permettant de surveiller des composes possedant des activites d'antifacteur xa et/ou d'antifacteur iia
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1978
- 1978-03-23 DE DE2812943A patent/DE2812943C3/de not_active Expired
1979
- 1979-01-16 EP EP79100122A patent/EP0004271A3/fr not_active Withdrawn
- 1979-01-16 AT AT32179A patent/AT362080B/de not_active IP Right Cessation
- 1979-01-31 AU AU43825/79A patent/AU512206B2/en not_active Ceased
- 1979-02-07 CA CA321,067A patent/CA1111334A/fr not_active Expired
- 1979-02-07 JP JP1234279A patent/JPS54128796A/ja active Granted
- 1979-02-07 DD DD79210876A patent/DD141952A5/de not_active IP Right Cessation
- 1979-02-08 IE IE238/79A patent/IE48067B1/en not_active IP Right Cessation
- 1979-02-12 DK DK57079A patent/DK149475C/da active
- 1979-02-13 ZA ZA79622A patent/ZA79622B/xx unknown
- 1979-02-13 HU HU79BO1763A patent/HU176068B/hu not_active IP Right Cessation
- 1979-02-13 FI FI790476A patent/FI62859C/fi not_active IP Right Cessation
- 1979-02-14 AR AR275507A patent/AR217503A1/es active
- 1979-02-21 US US06/013,759 patent/US4234682A/en not_active Expired - Lifetime
- 1979-03-20 CH CH261079A patent/CH642749A5/de not_active IP Right Cessation
- 1979-03-21 SE SE7902555A patent/SE445560B/sv not_active IP Right Cessation
- 1979-03-21 GB GB7909974A patent/GB2017298B/en not_active Expired
- 1979-03-22 BE BE194147A patent/BE875014A/fr not_active IP Right Cessation
- 1979-03-22 IT IT21220/79A patent/IT1112966B/it active
- 1979-03-22 NL NL7902259A patent/NL7902259A/xx active Search and Examination
- 1979-03-23 FR FR7907424A patent/FR2420764A1/fr active Granted
- 1979-03-23 LU LU81070A patent/LU81070A1/de unknown

Also Published As

Publication number	Publication date
AU4382579A (en)	1979-09-27
US4234682A (en)	1980-11-18
SE7902555L (sv)	1979-09-24
FR2420764B1 (fr)	1983-07-29
BE875014A (fr)	1979-09-24
IE48067B1 (en)	1984-09-19
AT362080B (de)	1981-04-27
IT7921220A0 (it)	1979-03-22
AU512206B2 (en)	1980-09-25
IE790238L (en)	1979-09-23
NL7902259A (nl)	1979-09-25
GB2017298A (en)	1979-10-03
LU81070A1 (de)	1979-06-19
IT1112966B (it)	1986-01-20
FI62859B (fi)	1982-11-30
ATA32179A (de)	1980-09-15
DE2812943A1 (de)	1979-10-04
HU176068B (en)	1980-12-28
DK57079A (da)	1979-09-24
JPS5726120B2 (fr)	1982-06-02
EP0004271A2 (fr)	1979-10-03
JPS54128796A (en)	1979-10-05
EP0004271A3 (fr)	1980-04-02
DE2812943C3 (de)	1981-05-14
CA1111334A (fr)	1981-10-27
DK149475B (da)	1986-06-23
FR2420764A1 (fr)	1979-10-19
FI790476A7 (fi)	1979-09-24
DE2812943B2 (de)	1980-08-14
GB2017298B (en)	1982-07-07
DK149475C (da)	1986-12-01
AR217503A1 (es)	1980-03-31
SE445560B (sv)	1986-06-30
ZA79622B (en)	1980-03-26
DD141952A5 (de)	1980-05-28
CH642749A5 (de)	1984-04-30

Legal Events

Date	Code	Title	Description
1993-02-13	MM	Patent lapsed
2004-08-31	MM	Patent lapsed	Owner name: BOEHRINGER MANNHEIM GMBH

Publication	Publication Date	Title
FI62859C (fi)	1983-03-10	Foerfarande och reagens foer bestaemning av heparinets biologiska aktivitet i plasma
Depasse et al.	2021	Thrombin generation assays are versatile tools in blood coagulation analysis: a review of technical features, and applications from research to laboratory routine
US4784944A (en)	1988-11-15	Prothrombin time determining reagent and methods of using and preparing the same
US20090087870A1 (en)	2009-04-02	Hematological assay and kit
Abildgaard	1968	Inhibition of the thrombin‐fibrinogen reaction by heparin and purified cofactor
EP2029768B1 (fr)	2013-02-13	Procédé permettant de déterminer l'activité de la thrombine dans plasma
Kahle et al.	1978	Antithrombin III I. Evaluation of an automated antithrombin III method
JP3533012B2 (ja)	2004-05-31	プロテインｃ／プロテインｓ系の障害を検出する方法
US5646007A (en)	1997-07-08	Method for determination of antithrombin III activity and reagent kit therefor
US4918001A (en)	1990-04-17	Method for the determination of protease inhibitors
Friberger	1983	Synthetic peptide substrate assays in coagulation and fibrinolysis and their application on automates
US5308756A (en)	1994-05-03	Protein S chromogenic assay
Barenghi et al.	1986	Measurement of erythrocyte acetylcholinesterase and plasma cholinesterase activity by a differential pH technique
JP3041457B2 (ja)	2000-05-15	因子ｉｘ発色アッセイ
EP0318571B1 (fr)	1993-03-17	Determination des constituants actifs dans une proteolyse
US8163513B2 (en)	2012-04-24	Method for determining the total clotting activity of a blood or plasma sample
EP0166690B1 (fr)	1987-10-21	Procédé et moyens analytiques pour déterminer la capacité du sérum à inhiber les protéases
Pierson-Perry et al.	1987	Development and characterization of an automated assay of effective heparin activity in plasma.
Hutton	1987	Chromogenic substrates in haemostasis
US5312745A (en)	1994-05-17	Determination of components active in proteolysis
Tripodi et al.	1981	A chromogenic assay of prothrombin compared with coagulation tests during anticoagulant therapy and liver disease
Mitchell	1983	Development and clinical use of automated synthetic substrate methods for the evaluation of coagulation and fibrinolysis
Heidtmann et al.	1990	Assay of functional activity of alpha 1-antichymotrypsin in plasma
Bartl et al.	1983	Application of several chromogenic substrate assays to automated instrumentation for coagulation analysis
Latallo	1980	Assessment of inhibitors with chromogenic substrates