FI85287C - Molekulaer kloning och expression i industriella mikro-organismarter. - Google Patents

Molekulaer kloning och expression i industriella mikro-organismarter. Download PDF

Info

Publication number: FI85287C
Authority: FI; Finland
Prior art keywords: industrial; cells; bacillus; dna; polypeptide
Prior art date: 1983-07-06

Application number

FI850845A

Other languages

English (en)

Finnish (fi)

Swedish (sv)

Other versions

FI850845A0 (fi

FI85287B (fi

FI850845L (fi

Inventor

Johan Pieter Marinus Sanders

Ee Jan Hendrik Van

Yvonne Johanna Vos

Den Berg Johannes Abel Van

Peter Michael Andreoli

Leo J S M Mulleners

Original Assignee

Gist Brocades Nv

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-07-06

Filing date

1985-03-01

Publication date

1992-03-25

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8190976&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=FI85287(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1985-03-01 Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv

1985-03-01 Publication of FI850845A0 publication Critical patent/FI850845A0/fi

1985-03-01 Publication of FI850845L publication Critical patent/FI850845L/fi

1991-12-13 Publication of FI85287B publication Critical patent/FI85287B/fi

1992-03-25 Application granted granted Critical

1992-03-25 Publication of FI85287C publication Critical patent/FI85287C/fi

Links

230000014509 gene expression Effects 0.000 title claims description 14
241000193830 Bacillus <bacterium> Species 0.000 claims description 76
108090000623 proteins and genes Proteins 0.000 claims description 67
239000013612 plasmid Substances 0.000 claims description 58
210000004027 cell Anatomy 0.000 claims description 42
238000000034 method Methods 0.000 claims description 40
108090000637 alpha-Amylases Proteins 0.000 claims description 27
229920001184 polypeptide Polymers 0.000 claims description 25
108090000765 processed proteins & peptides Proteins 0.000 claims description 25
102000004196 processed proteins & peptides Human genes 0.000 claims description 25
102000004139 alpha-Amylases Human genes 0.000 claims description 21
235000014469 Bacillus subtilis Nutrition 0.000 claims description 20
238000004519 manufacturing process Methods 0.000 claims description 19
102000004169 proteins and genes Human genes 0.000 claims description 19
210000001938 protoplast Anatomy 0.000 claims description 19
229940024171 alpha-amylase Drugs 0.000 claims description 17
229940088598 enzyme Drugs 0.000 claims description 17
230000004927 fusion Effects 0.000 claims description 17
239000002609 medium Substances 0.000 claims description 17
102000004190 Enzymes Human genes 0.000 claims description 16
108090000790 Enzymes Proteins 0.000 claims description 16
108091005804 Peptidases Proteins 0.000 claims description 14
244000005700 microbiome Species 0.000 claims description 13
239000004365 Protease Substances 0.000 claims description 12
230000009466 transformation Effects 0.000 claims description 11
108090000746 Chymosin Proteins 0.000 claims description 9
230000010076 replication Effects 0.000 claims description 8
239000013598 vector Substances 0.000 claims description 8
102000003960 Ligases Human genes 0.000 claims description 6
108090000364 Ligases Proteins 0.000 claims description 6
239000003795 chemical substances by application Substances 0.000 claims description 6
229940080701 chymosin Drugs 0.000 claims description 6
GNOLWGAJQVLBSM-UHFFFAOYSA-N n,n,5,7-tetramethyl-1,2,3,4-tetrahydronaphthalen-1-amine Chemical group C1=C(C)C=C2C(N(C)C)CCCC2=C1C GNOLWGAJQVLBSM-UHFFFAOYSA-N 0.000 claims description 6
108091008146 restriction endonucleases Proteins 0.000 claims description 6
230000029087 digestion Effects 0.000 claims description 4
230000030414 genetic transfer Effects 0.000 claims description 4
238000009655 industrial fermentation Methods 0.000 claims description 4
238000002271 resection Methods 0.000 claims description 4
101710163270 Nuclease Proteins 0.000 claims description 3
230000015572 biosynthetic process Effects 0.000 claims description 3
208000015181 infectious disease Diseases 0.000 claims description 3
210000000349 chromosome Anatomy 0.000 claims description 2
238000012239 gene modification Methods 0.000 claims description 2
230000005017 genetic modification Effects 0.000 claims description 2
235000013617 genetically modified food Nutrition 0.000 claims description 2
239000001963 growth medium Substances 0.000 claims description 2
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 3
238000010348 incorporation Methods 0.000 claims 2
230000000415 inactivating effect Effects 0.000 claims 1
230000001172 regenerating effect Effects 0.000 claims 1
230000003248 secreting effect Effects 0.000 claims 1
230000001131 transforming effect Effects 0.000 claims 1
108020004414 DNA Proteins 0.000 description 28
239000000047 product Substances 0.000 description 25
241000194108 Bacillus licheniformis Species 0.000 description 12
230000003115 biocidal effect Effects 0.000 description 12
102000035195 Peptidases Human genes 0.000 description 10
235000018102 proteins Nutrition 0.000 description 8
239000012634 fragment Substances 0.000 description 7
229920001817 Agar Polymers 0.000 description 6
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
239000008272 agar Substances 0.000 description 6
230000001105 regulatory effect Effects 0.000 description 6
238000000855 fermentation Methods 0.000 description 5
230000004151 fermentation Effects 0.000 description 5
238000003780 insertion Methods 0.000 description 5
230000037431 insertion Effects 0.000 description 5
239000000203 mixture Substances 0.000 description 5
241000233866 Fungi Species 0.000 description 4
102000012479 Serine Proteases Human genes 0.000 description 4
108010022999 Serine Proteases Proteins 0.000 description 4
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 4
230000001580 bacterial effect Effects 0.000 description 4
239000003139 biocide Substances 0.000 description 4
238000005119 centrifugation Methods 0.000 description 4
239000013611 chromosomal DNA Substances 0.000 description 4
239000003550 marker Substances 0.000 description 4
239000000243 solution Substances 0.000 description 4
239000006228 supernatant Substances 0.000 description 4
230000004083 survival effect Effects 0.000 description 4
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
241000894006 Bacteria Species 0.000 description 3
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
241000588724 Escherichia coli Species 0.000 description 3
108091092566 Extrachromosomal DNA Proteins 0.000 description 3
102000008072 Lymphokines Human genes 0.000 description 3
108010074338 Lymphokines Proteins 0.000 description 3
108010014251 Muramidase Proteins 0.000 description 3
102000016943 Muramidase Human genes 0.000 description 3
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 3
108091028043 Nucleic acid sequence Proteins 0.000 description 3
241000831652 Salinivibrio sharmensis Species 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
229930006000 Sucrose Natural products 0.000 description 3
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
239000007984 Tris EDTA buffer Substances 0.000 description 3
YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 3
238000010367 cloning Methods 0.000 description 3
238000010276 construction Methods 0.000 description 3
238000007796 conventional method Methods 0.000 description 3
230000000694 effects Effects 0.000 description 3
238000001962 electrophoresis Methods 0.000 description 3
238000000605 extraction Methods 0.000 description 3
239000000499 gel Substances 0.000 description 3
230000002068 genetic effect Effects 0.000 description 3
238000009396 hybridization Methods 0.000 description 3
230000010354 integration Effects 0.000 description 3
238000002955 isolation Methods 0.000 description 3
229960000274 lysozyme Drugs 0.000 description 3
239000004325 lysozyme Substances 0.000 description 3
235000010335 lysozyme Nutrition 0.000 description 3
238000010369 molecular cloning Methods 0.000 description 3
235000015097 nutrients Nutrition 0.000 description 3
238000012545 processing Methods 0.000 description 3
230000028327 secretion Effects 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
241000894007 species Species 0.000 description 3
239000005720 sucrose Substances 0.000 description 3
239000000725 suspension Substances 0.000 description 3
102000004506 Blood Proteins Human genes 0.000 description 2
108010017384 Blood Proteins Proteins 0.000 description 2
108091003079 Bovine Serum Albumin Proteins 0.000 description 2
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
108020004705 Codon Proteins 0.000 description 2
241000195493 Cryptophyta Species 0.000 description 2
108010059378 Endopeptidases Proteins 0.000 description 2
102000005593 Endopeptidases Human genes 0.000 description 2
102000018389 Exopeptidases Human genes 0.000 description 2
108010091443 Exopeptidases Proteins 0.000 description 2
108060003951 Immunoglobulin Proteins 0.000 description 2
108010050904 Interferons Proteins 0.000 description 2
102000014150 Interferons Human genes 0.000 description 2
125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 2
229930193140 Neomycin Natural products 0.000 description 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
108020004511 Recombinant DNA Proteins 0.000 description 2
102000006382 Ribonucleases Human genes 0.000 description 2
108010083644 Ribonucleases Proteins 0.000 description 2
240000004808 Saccharomyces cerevisiae Species 0.000 description 2
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
239000007983 Tris buffer Substances 0.000 description 2
150000001413 amino acids Chemical class 0.000 description 2
239000003242 anti bacterial agent Substances 0.000 description 2
229940088710 antibiotic agent Drugs 0.000 description 2
229940098773 bovine serum albumin Drugs 0.000 description 2
239000000872 buffer Substances 0.000 description 2
239000005018 casein Substances 0.000 description 2
BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 2
235000021240 caseins Nutrition 0.000 description 2
230000015556 catabolic process Effects 0.000 description 2
230000007910 cell fusion Effects 0.000 description 2
-1 chymosins Proteins 0.000 description 2
230000000052 comparative effect Effects 0.000 description 2
238000006731 degradation reaction Methods 0.000 description 2
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
229940066758 endopeptidases Drugs 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
230000002255 enzymatic effect Effects 0.000 description 2
238000010353 genetic engineering Methods 0.000 description 2
125000001475 halogen functional group Chemical group 0.000 description 2
238000003505 heat denaturation Methods 0.000 description 2
108010002430 hemicellulase Proteins 0.000 description 2
102000018358 immunoglobulin Human genes 0.000 description 2
229940072221 immunoglobulins Drugs 0.000 description 2
238000001114 immunoprecipitation Methods 0.000 description 2
238000000338 in vitro Methods 0.000 description 2
238000011534 incubation Methods 0.000 description 2
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
PGLTVOMIXTUURA-UHFFFAOYSA-N iodoacetamide Chemical compound NC(=O)CI PGLTVOMIXTUURA-UHFFFAOYSA-N 0.000 description 2
VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 2
229960004927 neomycin Drugs 0.000 description 2
229920002401 polyacrylamide Polymers 0.000 description 2
229940093429 polyethylene glycol 6000 Drugs 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
230000008929 regeneration Effects 0.000 description 2
238000011069 regeneration method Methods 0.000 description 2
239000002689 soil Substances 0.000 description 2
210000000130 stem cell Anatomy 0.000 description 2
VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
238000013518 transcription Methods 0.000 description 2
230000035897 transcription Effects 0.000 description 2
238000012546 transfer Methods 0.000 description 2
230000014616 translation Effects 0.000 description 2
108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
108020005065 3' Flanking Region Proteins 0.000 description 1
108020005029 5' Flanking Region Proteins 0.000 description 1
108010088237 ATCGAT-specific type II deoxyribonucleases Proteins 0.000 description 1
101710081719 Alpha-amylase B Proteins 0.000 description 1
229920000856 Amylose Polymers 0.000 description 1
241000228212 Aspergillus Species 0.000 description 1
241000304886 Bacilli Species 0.000 description 1
241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
241000276408 Bacillus subtilis subsp. subtilis str. 168 Species 0.000 description 1
108700003860 Bacterial Genes Proteins 0.000 description 1
108020004256 Beta-lactamase Proteins 0.000 description 1
101000914103 Bos taurus Chymosin Proteins 0.000 description 1
101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
241000222120 Candida <Saccharomycetales> Species 0.000 description 1
108010053835 Catalase Proteins 0.000 description 1
102000016938 Catalase Human genes 0.000 description 1
108010001857 Cell Surface Receptors Proteins 0.000 description 1
108010084185 Cellulases Proteins 0.000 description 1
102000005575 Cellulases Human genes 0.000 description 1
108020003215 DNA Probes Proteins 0.000 description 1
239000003298 DNA probe Substances 0.000 description 1
108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 1
101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
241000588722 Escherichia Species 0.000 description 1
108060002716 Exonuclease Proteins 0.000 description 1
102000001690 Factor VIII Human genes 0.000 description 1
108010054218 Factor VIII Proteins 0.000 description 1
241000193385 Geobacillus stearothermophilus Species 0.000 description 1
108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
102000004157 Hydrolases Human genes 0.000 description 1
108090000604 Hydrolases Proteins 0.000 description 1
108090001061 Insulin Proteins 0.000 description 1
102000004877 Insulin Human genes 0.000 description 1
241000235649 Kluyveromyces Species 0.000 description 1
208000002720 Malnutrition Diseases 0.000 description 1
206010034133 Pathogen resistance Diseases 0.000 description 1
108010073038 Penicillin Amidase Proteins 0.000 description 1
108010087702 Penicillinase Proteins 0.000 description 1
241000228143 Penicillium Species 0.000 description 1
239000002202 Polyethylene glycol Substances 0.000 description 1
108010059820 Polygalacturonase Proteins 0.000 description 1
241000589516 Pseudomonas Species 0.000 description 1
241000235070 Saccharomyces Species 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
108010071390 Serum Albumin Proteins 0.000 description 1
102000007562 Serum Albumin Human genes 0.000 description 1
241000187747 Streptomyces Species 0.000 description 1
108010022394 Threonine synthase Proteins 0.000 description 1
102000003978 Tissue Plasminogen Activator Human genes 0.000 description 1
108090000373 Tissue Plasminogen Activator Proteins 0.000 description 1
108700040099 Xylose isomerases Proteins 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
239000002671 adjuvant Substances 0.000 description 1
238000005273 aeration Methods 0.000 description 1
239000011543 agarose gel Substances 0.000 description 1
230000003625 amylolytic effect Effects 0.000 description 1
238000000137 annealing Methods 0.000 description 1
108010019077 beta-Amylase Proteins 0.000 description 1
102000005936 beta-Galactosidase Human genes 0.000 description 1
108010005774 beta-Galactosidase Proteins 0.000 description 1
102000006635 beta-lactamase Human genes 0.000 description 1
238000004166 bioassay Methods 0.000 description 1
229960000182 blood factors Drugs 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
229960005091 chloramphenicol Drugs 0.000 description 1
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
230000002759 chromosomal effect Effects 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
239000002299 complementary DNA Substances 0.000 description 1
230000021615 conjugation Effects 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
210000000805 cytoplasm Anatomy 0.000 description 1
231100000433 cytotoxic Toxicity 0.000 description 1
229940127089 cytotoxic agent Drugs 0.000 description 1
239000002254 cytotoxic agent Substances 0.000 description 1
231100000599 cytotoxic agent Toxicity 0.000 description 1
230000001472 cytotoxic effect Effects 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
239000003599 detergent Substances 0.000 description 1
102000004419 dihydrofolate reductase Human genes 0.000 description 1
238000007865 diluting Methods 0.000 description 1
239000006185 dispersion Substances 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
238000012869 ethanol precipitation Methods 0.000 description 1
102000013165 exonuclease Human genes 0.000 description 1
108010093305 exopolygalacturonase Proteins 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
229960000301 factor viii Drugs 0.000 description 1
238000001914 filtration Methods 0.000 description 1
230000002538 fungal effect Effects 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
230000007614 genetic variation Effects 0.000 description 1
238000011331 genomic analysis Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
239000008103 glucose Substances 0.000 description 1
229910001385 heavy metal Inorganic materials 0.000 description 1
229940088597 hormone Drugs 0.000 description 1
239000005556 hormone Substances 0.000 description 1
230000014726 immortalization of host cell Effects 0.000 description 1
230000036039 immunity Effects 0.000 description 1
230000006698 induction Effects 0.000 description 1
230000001939 inductive effect Effects 0.000 description 1
238000009776 industrial production Methods 0.000 description 1
238000001802 infusion Methods 0.000 description 1
239000003112 inhibitor Substances 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
229940125396 insulin Drugs 0.000 description 1
229940079322 interferon Drugs 0.000 description 1
229940047124 interferons Drugs 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
230000001071 malnutrition Effects 0.000 description 1
235000000824 malnutrition Nutrition 0.000 description 1
102000006240 membrane receptors Human genes 0.000 description 1
108020004999 messenger RNA Proteins 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000002703 mutagenesis Methods 0.000 description 1
231100000350 mutagenesis Toxicity 0.000 description 1
208000015380 nutritional deficiency disease Diseases 0.000 description 1
239000001814 pectin Substances 0.000 description 1
229920001277 pectin Polymers 0.000 description 1
235000010987 pectin Nutrition 0.000 description 1
239000008188 pellet Substances 0.000 description 1
238000007747 plating Methods 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
230000006798 recombination Effects 0.000 description 1
238000005215 recombination Methods 0.000 description 1
230000008439 repair process Effects 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
230000000284 resting effect Effects 0.000 description 1
230000007017 scission Effects 0.000 description 1
238000010187 selection method Methods 0.000 description 1
239000006152 selective media Substances 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
238000010998 test method Methods 0.000 description 1
229960000187 tissue plasminogen activator Drugs 0.000 description 1
230000005026 transcription initiation Effects 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
238000011426 transformation method Methods 0.000 description 1
238000013519 translation Methods 0.000 description 1
230000014621 translational initiation Effects 0.000 description 1
IEDVJHCEMCRBQM-UHFFFAOYSA-N trimethoprim Chemical compound COC1=C(OC)C(OC)=CC(CC=2C(=NC(N)=NC=2)N)=C1 IEDVJHCEMCRBQM-UHFFFAOYSA-N 0.000 description 1
229960001082 trimethoprim Drugs 0.000 description 1
230000035899 viability Effects 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
108010082737 zymolyase Proteins 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Compounds Of Unknown Constitution (AREA)

FI850845A 1983-07-06 1985-03-01 Molekulaer kloning och expression i industriella mikro-organismarter. FI85287C (fi)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
EP83201016		1983-07-06
EP83201016		1983-07-06
PCT/NL1984/000021 WO1985000382A1 (en)	1983-07-06	1984-07-06	Molecular cloning and expression in industrial microorganism species
NL8400021		1984-07-06

Publications (4)

Publication Number	Publication Date
FI850845A0 FI850845A0 (fi)	1985-03-01
FI850845L FI850845L (fi)	1985-03-01
FI85287B FI85287B (fi)	1991-12-13
FI85287C true FI85287C (fi)	1992-03-25

Family

ID=8190976

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
FI850845A FI85287C (fi)	1983-07-06	1985-03-01	Molekulaer kloning och expression i industriella mikro-organismarter.

Country Status (11)

Country	Link
EP (1)	EP0134048B2 (lt)
JP (2)	JP2669457B2 (lt)
AU (1)	AU570709B2 (lt)
DE (1)	DE3480411D1 (lt)
DK (1)	DK169996B1 (lt)
ES (2)	ES8608579A1 (lt)
FI (1)	FI85287C (lt)
GR (1)	GR82200B (lt)
IE (1)	IE58014B1 (lt)
NZ (1)	NZ208806A (lt)
WO (1)	WO1985000382A1 (lt)

Families Citing this family (105)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5624829A (en) *	1984-07-03	1997-04-29	Gist-Brocades, B.V.	Transformed industrial bacillus strains and methods for making and using them
CA1311429C (en) *	1983-07-06	1992-12-15	Vasantha Nagarajan	Vector for polypeptides in bacilli
EP0149241B1 (en) *	1983-12-28	1991-03-27	Agency Of Industrial Science And Technology	Dna base sequence containing regions involved in the production and secretion of a protein, recombinant dna including the whole or a part of the dna base sequence, and method of producing proteins by use of the recombinant dna
JPS60188093A (ja) *	1984-03-09	1985-09-25	Teruhiko Beppu	枯草菌に於ける形質発現プラスミド
IE58099B1 (en) *	1984-06-06	1993-06-30	Cpc International Inc	Amylase-negative, asporogenous mutant of bacillus subtilis useful as a host in a host-vector system
US4801537A (en) *	1984-06-08	1989-01-31	Genex Corporation	Vector for expression of polypeptides in bacilli
JPH0616711B2 (ja) *	1985-09-27	1994-03-09	メルシャン株式会社	高温で自律増殖するプラスミド
US5024943A (en) *	1985-11-07	1991-06-18	Gist-Brocades	Regulatory region cloning and analysis plasmid for bacillus
DK311186D0 (da) *	1986-06-30	1986-06-30	Novo Industri As	Enzymer
BR8805647A (pt) *	1987-02-27	1989-10-31	Gist Brocades Nv	Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
AU620326B2 (en) *	1987-02-27	1992-02-20	Dsm Ip Assets B.V.	Stable gene amplification in chromosomal dna of prokaryotic microorganisms
ES2135386T3 (es) *	1987-02-27	1999-11-01	Genencor Int	Transformacion de cepas de bacillus alcalofilas.
CA2030554C (en) *	1989-06-29	2001-08-28	Wilhelmus J. Quax	Mutant microbial .alpha.-amylases with increased thermal, acid, and/or alkaline stability
DE4216246A1 (de) *	1992-05-16	1993-11-18	Solvay Enzymes Gmbh & Co Kg	Einfaches Verfahren zur stabilen chromosomalen Genamplifikation
GB9907461D0 (en)	1999-03-31	1999-05-26	King S College London	Neurite regeneration
US8372620B2 (en)	1998-12-23	2013-02-12	Dupont Nutrition Biosciences Aps	Bacterial xylanases
GB0020498D0 (en)	2000-08-18	2000-10-11	Sterix Ltd	Compound
ES2394481T3 (es)	2000-09-21	2013-02-01	Basf Se	Xilanasa de talaromyces
ATE475324T1 (de)	2000-12-07	2010-08-15	Dsm Ip Assets Bv	Mit carboxy-terminal prolin peptide angereicherte proteinhydrolysate
CA2706364A1 (en)	2000-12-07	2002-06-13	Dsm Ip Assets B.V.	Method for the prevention or reduction of haze in beverages
GB0116453D0 (en)	2001-07-05	2001-08-29	Imp College Innovations Ltd	Method
DK1495128T3 (da)	2002-03-29	2014-08-11	Genencor Int	Forstærket proteinekspression i Bacillus
AU2003228145B2 (en)	2002-06-07	2009-10-08	Dsm Ip Assets B.V.	Improved method for the prevention or reduction of haze in beverages
TWI328612B (en)	2002-07-25	2010-08-11	Dsm Ip Assets Bv	Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
EP1625202A4 (en)	2003-05-12	2010-10-20	Genencor Int	NEW LIPOLYTIC ENZYME LIP1
GB0403864D0 (en)	2004-02-20	2004-03-24	Ucl Ventures	Modulator
ES2454167T3 (es)	2005-11-28	2014-04-09	Dsm Ip Assets B.V.	Preparación de enzima que produce un sabor puro
GB0600406D0 (en)	2006-01-10	2006-02-15	Univ Bath	Crystal
BRPI0807768A2 (pt)	2007-02-20	2014-06-24	Dsm Ip Assests Bv	Nova sialidase.
CN103667323B (zh)	2007-03-12	2016-02-03	丹尼斯科美国公司	经修饰的蛋白酶
JP2010521188A (ja)	2007-03-22	2010-06-24	ディーエスエムアイピーアセッツビー．ブイ．	新規リシルオキシダーゼ
WO2008141281A1 (en)	2007-05-10	2008-11-20	Danisco Us Inc., Genencor Division	A modified secretion system to increase expression of polypeptides in bacteria
GB0715751D0 (en) *	2007-08-13	2007-09-19	Tmo Renewables Ltd	Thermophilic micro-organisms for ethanol production
CA2709804A1 (en)	2007-12-21	2009-07-30	Danisco Us Inc.	Enhanced protein production in bacillus
JP5659143B2 (ja)	2008-03-28	2015-01-28	ダニスコ・ユーエス・インク	細菌性細胞中の遺伝子座を増幅する方法
US8530218B2 (en)	2009-04-24	2013-09-10	Danisco Us Inc.	Proteases with modified pro regions
AR076941A1 (es)	2009-06-11	2011-07-20	Danisco Us Inc	Cepa de bacillus para una mayor produccion de proteina
US8728790B2 (en)	2009-12-09	2014-05-20	Danisco Us Inc.	Compositions and methods comprising protease variants
WO2011101339A2 (en)	2010-02-18	2011-08-25	Dsm Ip Assets B.V.	Fermentation broth and filtration filter cake and uses thereof
WO2011130222A2 (en)	2010-04-15	2011-10-20	Danisco Us Inc.	Compositions and methods comprising variant proteases
EP3095861B1 (en)	2010-05-06	2019-06-26	The Procter and Gamble Company	Consumer products with protease variants
WO2012007445A1 (en)	2010-07-13	2012-01-19	Dsm Ip Assets B.V.	Mutarotase in crystallization
WO2012007446A1 (en)	2010-07-13	2012-01-19	Dsm Ip Assets B.V.	Use of a mutarotase in the production of dried powders
CN103764823B (zh)	2011-05-05	2018-05-11	丹尼斯科美国公司	包含丝氨酸蛋白酶变体的组合物和方法
JP5933688B2 (ja)	2011-05-05	2016-06-15	ザプロクターアンドギャンブルカンパニー	セリンプロテアーゼ変異体を含む組成物及び方法
CN102296042B (zh) *	2011-08-26	2013-04-24	甘肃农业大学	地衣芽孢杆菌及用其制备凝乳酶冻干粉的方法
AR087745A1 (es)	2011-08-31	2014-04-16	Danisco Us Inc	Composiciones y metodos que comprenden una variante de enzima lipolitica
US20150017700A1 (en)	2011-12-22	2015-01-15	Danisco Us Inc.	Compositions and methods comprising a lipolytic enzyme variant
HK1209785A1 (en)	2012-10-12	2016-04-08	Danisco Us Inc.	Compositions and methods comprising a lipolytic enzyme variant
KR20150082502A (ko)	2012-11-05	2015-07-15	다니스코 유에스 인크.	써몰리신 프로테아제 변이체를 포함하는 조성물 및 방법
US20150344858A1 (en)	2012-12-19	2015-12-03	Danisco Us Inc.	Novel mannanase, compositions and methods of use thereof
WO2014194034A2 (en)	2013-05-29	2014-12-04	Danisco Us Inc.	Novel metalloproteases
DK3882346T3 (da)	2013-05-29	2025-12-22	Danisco Us Inc	Hidtil ukendte metalloproteaser
WO2014194032A1 (en)	2013-05-29	2014-12-04	Danisco Us Inc.	Novel metalloproteases
EP3004342B1 (en)	2013-05-29	2023-01-11	Danisco US Inc.	Novel metalloproteases
WO2015010009A2 (en)	2013-07-19	2015-01-22	Danisco Us Inc.	Compositions and methods comprising a lipolytic enzyme variant
WO2015038792A1 (en)	2013-09-12	2015-03-19	Danisco Us Inc.	Compositions and methods comprising lg12-clade protease variants
US20160312204A1 (en)	2013-12-13	2016-10-27	Danisco Us Inc.	Serine proteases of bacillus species
EP3080263B1 (en)	2013-12-13	2019-07-03	Danisco US Inc.	Serine proteases of the bacillus gibsonii-clade
EP3089991B1 (en)	2013-12-31	2019-08-28	Danisco US Inc.	Enhanced protein expression
MX2016012044A (es)	2014-03-21	2017-06-29	Danisco Us Inc	Serina proteasas de especies de bacillus.
DK3207129T3 (da)	2014-10-17	2020-02-24	Danisco Us Inc	Serinproteaser af bacillus-arten
WO2016069569A2 (en)	2014-10-27	2016-05-06	Danisco Us Inc.	Serine proteases
CN107148472A (zh)	2014-10-27	2017-09-08	丹尼斯科美国公司	芽孢杆菌属物种的丝氨酸蛋白酶
DK3212662T3 (da)	2014-10-27	2020-07-20	Danisco Us Inc	Serinproteaser
EP3212783B1 (en)	2014-10-27	2024-06-26	Danisco US Inc.	Serine proteases
EP3212781B1 (en)	2014-10-27	2019-09-18	Danisco US Inc.	Serine proteases
KR102588719B1 (ko)	2014-12-16	2023-10-12	다니스코 유에스 인크.	향상된 단백질 발현
WO2016100128A1 (en)	2014-12-19	2016-06-23	Danisco Us Inc	Enhanced protein expression
DK3268471T3 (da)	2015-03-12	2019-12-02	Danisco Us Inc	Sammensætninger og fremgangsmåder omfattende lg12-clade-proteasevarianter
EP4219704A3 (en)	2015-05-13	2023-08-23	Danisco US Inc	Aprl-clade protease variants and uses thereof
JP7015695B2 (ja)	2015-06-17	2022-02-03	ダニスコ・ユーエス・インク	バチルス・ギブソニイ（Ｂａｃｉｌｌｕｓｇｉｂｓｏｎｉｉ）クレードセリンプロテアーゼ
US10731144B2 (en)	2015-06-17	2020-08-04	Danisco Us Inc	Proteases with modified propeptide regions
BR112018072530A2 (pt)	2016-05-03	2019-03-26	Danisco Us Inc	variantes de protease e usos das mesmas
BR112018072586A2 (pt)	2016-05-05	2019-02-19	Danisco Us Inc	variantes de protease e usos das mesmas
CA3026064A1 (en)	2016-05-31	2017-12-07	Danisco Us Inc.	Protease variants and uses thereof
CA3027745A1 (en)	2016-06-17	2017-12-21	Danisco Us Inc.	Protease variants and uses thereof
EP4520820A3 (en)	2016-12-21	2025-04-16	Danisco Us Inc	Protease variants and uses thereof
US11946081B2 (en)	2016-12-21	2024-04-02	Danisco Us Inc.	Bacillus gibsonii-clade serine proteases
EP3717643A1 (en)	2017-11-29	2020-10-07	Danisco US Inc.	Subtilisin variants having improved stability
EP3799601A1 (en)	2018-06-19	2021-04-07	Danisco US Inc.	Subtilisin variants
WO2019245704A1 (en)	2018-06-19	2019-12-26	Danisco Us Inc	Subtilisin variants
WO2020046613A1 (en)	2018-08-30	2020-03-05	Danisco Us Inc	Compositions comprising a lipolytic enzyme variant and methods of use thereof
EP3887515A1 (en)	2018-11-28	2021-10-06	Danisco US Inc.	Subtilisin variants having improved stability
CN114174504B (zh)	2019-05-24	2026-04-17	丹尼斯科美国公司	枯草杆菌蛋白酶变体和使用方法
US20230049452A1 (en)	2020-01-13	2023-02-16	Danisco Us Inc	Compositions comprising a lipolytic enzyme variant and methods of use thereof
US20240034960A1 (en)	2020-08-27	2024-02-01	Danisco Us Inc	Enzymes and enzyme compositions for cleaning
WO2023278297A1 (en)	2021-06-30	2023-01-05	Danisco Us Inc	Variant lipases and uses thereof
EP4396320A2 (en)	2021-09-03	2024-07-10	Danisco US Inc.	Laundry compositions for cleaning
EP4448751A2 (en)	2021-12-16	2024-10-23	Danisco US Inc.	Subtilisin variants and methods of use
JP7842216B2 (ja)	2021-12-16	2026-04-07	ザプロクターアンドギャンブルカンパニー	プロテアーゼを含む自動食器洗浄組成物
EP4448750A2 (en)	2021-12-16	2024-10-23	Danisco US Inc.	Subtilisin variants and uses thereof
CA3240638A1 (en)	2021-12-16	2023-06-22	Michelle Jackson	Fabric and home care composition comprising a protease
WO2023114939A2 (en)	2021-12-16	2023-06-22	Danisco Us Inc.	Subtilisin variants and methods of use
WO2023168234A1 (en)	2022-03-01	2023-09-07	Danisco Us Inc.	Enzymes and enzyme compositions for cleaning
CN120112635A (zh)	2022-09-02	2025-06-06	丹尼斯科美国公司	枯草杆菌蛋白酶变体及其相关方法
EP4581119A1 (en)	2022-09-02	2025-07-09	Danisco US Inc.	Detergent compositions and methods related thereto
CN120303400A (zh)	2022-11-09	2025-07-11	丹尼斯科美国公司	枯草杆菌蛋白酶变体和使用方法
WO2024163584A1 (en)	2023-02-01	2024-08-08	Danisco Us Inc.	Subtilisin variants and methods of use
JP2026503673A (ja)	2023-02-01	2026-01-29	ザプロクターアンドギャンブルカンパニー	酵素を含有する洗剤組成物
EP4677080A1 (en)	2023-03-06	2026-01-14	Danisco US Inc.	Subtilisin variants and methods of use
WO2025071996A1 (en)	2023-09-28	2025-04-03	Danisco Us Inc.	Variant cutinase enzymes with improved solubility and uses thereof
WO2025085351A1 (en)	2023-10-20	2025-04-24	Danisco Us Inc.	Subtilisin variants and methods of use
WO2026024921A1 (en)	2024-07-25	2026-01-29	The Procter & Gamble Company	Detergent composition comprising a subtilisin variant and methods of use
WO2026050315A1 (en)	2024-08-29	2026-03-05	Danisco Us Inc.	Subtilisin variants and methods of use

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
NO159863C (no) *	1980-01-07	1989-02-15	Univ Rochester	Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme.
ZA81424B (en) *	1980-02-15	1982-02-24	Cpc International Inc	Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same
IL61982A (en) *	1980-02-15	1984-01-31	Cpc International Inc	Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
AU545912B2 (en) *	1980-03-10	1985-08-08	Cetus Corporation	Cloned heterologous jive products in bacillies
JPS56137896A (en) *	1980-03-10	1981-10-28	Cetus Corp	Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria
FI64813C (fi) *	1980-12-31	1984-01-10	Ilkka Antero Palva	Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
CA1206108A (en) *	1981-03-06	1986-06-17	Shuichi Aiba	Process for transforming microorganism by means of plasmid introduction
BR8205954A (pt) *	1981-10-14	1983-09-13	Unilever Nv	Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
JPS5889194A (ja) *	1981-11-24	1983-05-27	Ajinomoto Co Inc	微生物によるｌ−トリプトフアンの製造法
JPS58107192A (ja) *	1981-12-18	1983-06-25	Ajinomoto Co Inc	発酵法によるｌ−ヒスチジンの製造法

1984
- 1984-07-06 IE IE172684A patent/IE58014B1/en not_active IP Right Cessation
- 1984-07-06 GR GR75238A patent/GR82200B/el unknown
- 1984-07-06 DE DE8484200990T patent/DE3480411D1/de not_active Expired
- 1984-07-06 NZ NZ20880684A patent/NZ208806A/xx unknown
- 1984-07-06 EP EP19840200990 patent/EP0134048B2/en not_active Expired - Lifetime
- 1984-07-06 JP JP59502729A patent/JP2669457B2/ja not_active Expired - Fee Related
- 1984-07-06 AU AU31510/84A patent/AU570709B2/en not_active Expired
- 1984-07-06 WO PCT/NL1984/000021 patent/WO1985000382A1/en not_active Ceased
- 1984-07-06 ES ES534102A patent/ES8608579A1/es not_active Expired
1985
- 1985-03-01 FI FI850845A patent/FI85287C/fi not_active IP Right Cessation
- 1985-03-05 DK DK101385A patent/DK169996B1/da not_active IP Right Cessation
- 1985-07-16 ES ES545256A patent/ES8609467A1/es not_active Expired
1993
- 1993-10-27 JP JP5269181A patent/JP2664860B2/ja not_active Expired - Fee Related

Also Published As

Publication number	Publication date
ES8609467A1 (es)	1986-08-01
EP0134048B1 (en)	1989-11-08
WO1985000382A1 (en)	1985-01-31
FI850845A0 (fi)	1985-03-01
FI85287B (fi)	1991-12-13
AU570709B2 (en)	1988-03-24
ES545256A0 (es)	1986-08-01
GR82200B (lt)	1984-12-13
JP2664860B2 (ja)	1997-10-22
FI850845L (fi)	1985-03-01
DK101385A (da)	1985-03-05
JPS60501738A (ja)	1985-10-17
DE3480411D1 (en)	1989-12-14
EP0134048A1 (en)	1985-03-13
DK101385D0 (da)	1985-03-05
IE841726L (en)	1985-01-06
ES534102A0 (es)	1986-06-16
JP2669457B2 (ja)	1997-10-27
EP0134048B2 (en)	1996-08-14
ES8608579A1 (es)	1986-06-16
AU3151084A (en)	1985-02-07
NZ208806A (en)	1988-07-28
DK169996B1 (da)	1995-04-24
IE58014B1 (en)	1993-06-16
JPH06189783A (ja)	1994-07-12

Legal Events

Date	Code	Title	Description
1998-12-28	PC	Transfer of assignment of patent	Owner name: DSM N.V.
2004-08-31	MA	Patent expired	Owner name: DSM N.V.

Publication	Publication Date	Title
FI85287C (fi)	1992-03-25	Molekulaer kloning och expression i industriella mikro-organismarter.
RU2091487C1 (ru)	1997-09-27	Способ получения штамма bacillus, содержащего две копии последовательности днк, кодирующей фермент с гидролазной активностью
EP0479396B1 (en)	1999-06-09	Transformation of alkalophilic bacillus strains
FI105483B (fi)	2000-08-31	Menetelmä kiinnostavan endogeenisen entsyymin tuotannon tehostamiseksi alkalofiilisessa Bacillus-isäntäsolussa
EP0127328A2 (en)	1984-12-05	The use of chromosomal integration to stabilize heterologous genes
US5624829A (en)	1997-04-29	Transformed industrial bacillus strains and methods for making and using them
US5238833A (en)	1993-08-24	Molecular cloning and expression in industrial Bacillus species
JP3013008B2 (ja)	2000-02-28	無胞子性菌株バシラス・サチリスｓｍｓ２７５