HUP0100079A2 - Új, fluoreszcens riportermolekulák és alkalmazásaik, többek között kaszpázok vizsgálatára - Google Patents

Új, fluoreszcens riportermolekulák és alkalmazásaik, többek között kaszpázok vizsgálatára

Info

Publication number
HUP0100079A2
HUP0100079A2 HU0100079A HUP0100079A HUP0100079A2 HU P0100079 A2 HUP0100079 A2 HU P0100079A2 HU 0100079 A HU0100079 A HU 0100079A HU P0100079 A HUP0100079 A HU P0100079A HU P0100079 A2 HUP0100079 A2 HU P0100079A2
Authority
HU
Hungary
Prior art keywords
cells
ass
rhodamine
compound
test
Prior art date
Application number
HU0100079A
Other languages
English (en)
Hungarian (hu)
Inventor
Sui Xiong Cai
John A. Drewe
John F. W. Keana
Eckard Weber
Han-Zhong Zhang
Original Assignee
Cytovia, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cytovia, Inc. filed Critical Cytovia, Inc.
Publication of HUP0100079A2 publication Critical patent/HUP0100079A2/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K9/00—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06026—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atom, i.e. Gly or Ala
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06034—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms
    • C07K5/06052—Val-amino acid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/0806—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/0808—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms, e.g. Val, Ile, Leu
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1005—Tetrapeptides with the first amino acid being neutral and aliphatic
    • C07K5/101—Tetrapeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms, e.g. Val, Ile, Leu
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1005—Tetrapeptides with the first amino acid being neutral and aliphatic
    • C07K5/1013—Tetrapeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1016—Tetrapeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1021—Tetrapeptides with the first amino acid being acidic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1488—Methods for deciding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
HU0100079A 1997-10-10 1998-10-09 Új, fluoreszcens riportermolekulák és alkalmazásaik, többek között kaszpázok vizsgálatára HUP0100079A2 (hu)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6158297P 1997-10-10 1997-10-10
US3366198A 1998-03-03 1998-03-03
PCT/US1998/021231 WO1999018856A1 (fr) 1997-10-10 1998-10-09 Nouvelles molecules reporters fluorescentes, applications de ces molecules et dosages des caspases

Publications (1)

Publication Number Publication Date
HUP0100079A2 true HUP0100079A2 (hu) 2001-05-28

Family

ID=26709974

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0100079A HUP0100079A2 (hu) 1997-10-10 1998-10-09 Új, fluoreszcens riportermolekulák és alkalmazásaik, többek között kaszpázok vizsgálatára

Country Status (15)

Country Link
EP (1) EP1026988A4 (fr)
JP (1) JP2001519368A (fr)
KR (1) KR20010031056A (fr)
CN (1) CN1281346A (fr)
AU (1) AU754634B2 (fr)
BR (1) BR9814816A (fr)
CA (1) CA2308125A1 (fr)
EA (1) EA200000408A1 (fr)
HU (1) HUP0100079A2 (fr)
IL (1) IL135365A0 (fr)
IS (1) IS5414A (fr)
NO (1) NO20001322L (fr)
NZ (1) NZ503619A (fr)
PL (1) PL341661A1 (fr)
WO (1) WO1999018856A1 (fr)

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EP1100520B1 (fr) 1998-07-21 2004-09-22 Cytovia, Inc. Nouveaux colorants fluorescents et leurs applications dans des methodes de criblage par fluorescence de cellules entieres pour des caspases, peptidases, proteases et autres enzymes ainsi que leurs procedes d' utilisation
DE50003728D1 (en) * 1999-03-12 2003-10-23 Evotec Technologies Gmbh Chemosensitivitätsmessung über caspase-aktivität
EP1055929A1 (fr) * 1999-05-26 2000-11-29 Tibotec N.V. Moyens et procédé pour la découverte des médicaments et la caractérisation des cellules
AU4906100A (en) * 1999-05-27 2000-12-18 Merck Frosst Canada & Co. Assays for caspase activity using green fluorescent proteins
US6261794B1 (en) * 1999-10-14 2001-07-17 Saint Louis University Methods for identifying inhibitors of methionine aminopeptidases
AU781986B2 (en) 1999-11-05 2005-06-23 Cytovia, Inc. Substituted 4H-chromene and analogs as activators of caspases and inducers of apoptosis and the use thereof
GB9927331D0 (en) 1999-11-18 2000-01-12 Fluorescience Ltd Assay for measuring enzyme activity in vivo
EP1324993A2 (fr) 2000-04-18 2003-07-09 Cytovia, Inc. Thiazepine-1,4 substituee et analogues de celle-ci activateurs de caspases et inducteurs d'apoptose, et utilisation
WO2002012545A2 (fr) * 2000-08-03 2002-02-14 Cytovia, Inc. Procédé d'identification d'agents immunosuppresseurs
DE10053747C2 (de) * 2000-10-30 2002-10-24 Deutsches Krebsforsch Verfahren zur gleichzeitigen Bestimmung zweier Fluoreszenz-Emissionen mit einem Einlaser-Durchflußzytometer
EP1205540A1 (fr) 2000-11-10 2002-05-15 Evotec OAI AG Procédé pour déterminer la vitalité de cellules
WO2002102301A2 (fr) 2000-12-07 2002-12-27 Cytovia, Inc. Benzylidene-hydrazides d'acide indole-2-carboxylique substitues et analogues utilises comme activateurs de caspases et inducteurs d'apoptose, et leur utilisation
US6716851B2 (en) 2000-12-12 2004-04-06 Cytovia, Inc. Substituted 2-aryl-4-arylaminopyrimidines and analogs as activators or caspases and inducers of apoptosis and the use thereof
US7256219B2 (en) 2001-03-14 2007-08-14 Cytovia, Inc. Multifluoro-substituted chalcones and analogs as activators of caspases and inducers of apoptosis and the use thereof
US6979530B2 (en) 2001-05-21 2005-12-27 Applera Corporation Peptide conjugates and fluorescence detection methods for intracellular caspase assay
EP1472238B1 (fr) 2002-02-01 2012-07-04 Promega Corporation Dosage bioluminescent de la protease
US7476741B2 (en) 2002-05-16 2009-01-13 Cytovia, Inc. Substituted 4H-chromens, 2H-chromenes, chromans and analogs as activators of caspases and inducers of apoptosis and the use thereof
CN100349887C (zh) 2002-05-16 2007-11-21 西托维亚公司 取代的4－芳基－4h－吡咯并[2,3－h]色烯和类似物及其药物组合物和用途
DE60235652D1 (de) * 2002-07-08 2010-04-22 Ge Healthcare Uk Ltd Reagenzien sowie ein verfahren zur sättigungsmarkierung von proteinen
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CA2531327A1 (fr) 2003-07-03 2005-01-13 Myriad Genetics, Inc. Composes et leur utilisation therapeutique
MXPA06001601A (es) 2003-08-13 2006-08-25 Takeda Pharmaceutical Derivados de 4-pirimidona y su uso como inhibidores de dipeptidilpeptidasa.
WO2005047245A2 (fr) 2003-11-14 2005-05-26 Wisconsin Alumni Research Foundation Dosages par fluorescence à sensibilité améliorée
EP1704257A2 (fr) 2004-01-16 2006-09-27 Applera Corporation Essais fluorogeniques pour kinase et substrats
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CN104458709A (zh) * 2013-09-12 2015-03-25 中国药科大学 一种筛选钙激活中性蛋白酶-1抑制剂高通量筛选方法
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CN104263356B (zh) * 2014-09-29 2016-02-24 辽宁大学 一种含有甲硫氨酸结构的罗丹明pH荧光探针及其应用
CN106146611B (zh) * 2015-05-14 2019-09-17 中国科学院大连化学物理研究所 一种测定二肽基肽酶iv活性的荧光探针底物及其应用
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WO2018071556A1 (fr) 2016-10-11 2018-04-19 The Regents Of The University Of California Détection, identification et purification d'enzymes de dégradation et de non dégradation dans des échantillons biologiques
CN107987019A (zh) * 2018-01-08 2018-05-04 深圳市佶达德科技有限公司 一种若丹明双吡唑乙酸酯有机激光材料的制备方法
CN108129388A (zh) * 2018-01-08 2018-06-08 深圳市佶达德科技有限公司 一种若丹明双吡唑乙酸酯有机激光材料及其应用
CN108760234B (zh) * 2018-06-05 2020-05-15 哈尔滨工程大学 一种基于piv和ptv技术的流体流动与固体运动信息同步测试方法和装置
KR102138256B1 (ko) * 2018-09-13 2020-08-13 한국과학기술연구원 케스페이즈-1 활성 측정용 프로브, 이를 포함하는 염증성 질환 진단용 조성물
CN112961209B (zh) * 2021-04-07 2022-06-24 中国科学院武汉病毒研究所 caspase抑制剂及其应用
CN114107435A (zh) * 2021-11-30 2022-03-01 广东省人民医院 一种用于免疫治疗实时监测的可激活光声-荧光双模探针及应用
CN117143949B (zh) * 2023-08-31 2024-04-05 青岛海洋食品营养与健康创新研究院 一种南极磷虾源高f值寡肽及其在护肝中的应用
CN121449684A (zh) * 2025-10-30 2026-02-03 浙江大学 一种基于FRET的caspase-1响应性荧光探针、制备方法、应用及产品

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Also Published As

Publication number Publication date
AU754634B2 (en) 2002-11-21
AU1072299A (en) 1999-05-03
JP2001519368A (ja) 2001-10-23
KR20010031056A (ko) 2001-04-16
NO20001322L (no) 2000-06-13
EA200000408A1 (ru) 2000-12-25
IL135365A0 (en) 2001-05-20
CN1281346A (zh) 2001-01-24
EP1026988A1 (fr) 2000-08-16
EP1026988A4 (fr) 2005-03-30
PL341661A1 (en) 2001-04-23
IS5414A (is) 2000-03-24
NO20001322D0 (no) 2000-03-14
WO1999018856A1 (fr) 1999-04-22
NZ503619A (en) 2001-11-30
BR9814816A (pt) 2004-06-22
CA2308125A1 (fr) 1999-04-22

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