IE48321B1 - Pretreatment of microcarriers for cell culture - Google Patents
Pretreatment of microcarriers for cell cultureInfo
- Publication number
- IE48321B1 IE48321B1 IE598/79A IE59879A IE48321B1 IE 48321 B1 IE48321 B1 IE 48321B1 IE 598/79 A IE598/79 A IE 598/79A IE 59879 A IE59879 A IE 59879A IE 48321 B1 IE48321 B1 IE 48321B1
- Authority
- IE
- Ireland
- Prior art keywords
- beads
- fcs
- cells
- microcarriers
- calf serum
- Prior art date
Links
- 238000004113 cell culture Methods 0.000 title 1
- 239000011324 bead Substances 0.000 claims abstract description 40
- MZVQCMJNVPIDEA-UHFFFAOYSA-N [CH2]CN(CC)CC Chemical group [CH2]CN(CC)CC MZVQCMJNVPIDEA-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229920002307 Dextran Polymers 0.000 claims abstract description 3
- 238000005349 anion exchange Methods 0.000 claims abstract description 3
- 239000011159 matrix material Substances 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 2
- 210000002966 serum Anatomy 0.000 claims 2
- 244000309466 calf Species 0.000 claims 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 abstract description 24
- 239000012894 fetal calf serum Substances 0.000 abstract description 24
- 241000251539 Vertebrata <Metazoa> Species 0.000 abstract description 2
- 230000001419 dependent effect Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 1
- 210000001840 diploid cell Anatomy 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 210000003837 chick embryo Anatomy 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 239000007757 Media 199 Substances 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 239000003957 anion exchange resin Substances 0.000 description 1
- 239000002800 charge carrier Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Farming Of Fish And Shellfish (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US88513778A | 1978-03-10 | 1978-03-10 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE790598L IE790598L (en) | 1979-09-10 |
| IE48321B1 true IE48321B1 (en) | 1984-12-12 |
Family
ID=25386226
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE598/79A IE48321B1 (en) | 1978-03-10 | 1979-08-08 | Pretreatment of microcarriers for cell culture |
Country Status (12)
| Country | Link |
|---|---|
| JP (1) | JPS54129184A (da) |
| BE (1) | BE874740A (da) |
| CH (1) | CH640264A5 (da) |
| DE (1) | DE2909340A1 (da) |
| DK (1) | DK98679A (da) |
| FR (1) | FR2419321A1 (da) |
| GB (1) | GB2016044B (da) |
| IE (1) | IE48321B1 (da) |
| IT (1) | IT7948280A0 (da) |
| LU (1) | LU81018A1 (da) |
| NL (1) | NL7901916A (da) |
| SE (1) | SE7902140L (da) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2470794A1 (fr) * | 1979-12-05 | 1981-06-12 | Pasteur Institut | Nouvelles microparticules, leur preparation et leurs applications en biologie, notamment a la culture de cellules diploides humaines |
| FR2543158B1 (fr) * | 1983-03-24 | 1985-11-15 | Inst Nat Sante Rech Med | Milieu de culture de cellules animales sans serum, sans hormones et sans facteurs de croissance et procedes de culture primaire et d'obtention de lignees cellulaires utilisant ce milieu |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4036693A (en) * | 1976-02-02 | 1977-07-19 | Massachusetts Institute Of Technology | Treatment of cell culture microcarries |
-
1979
- 1979-03-08 LU LU81018A patent/LU81018A1/xx unknown
- 1979-03-08 FR FR7905985A patent/FR2419321A1/fr active Granted
- 1979-03-09 GB GB7908389A patent/GB2016044B/en not_active Expired
- 1979-03-09 NL NL7901916A patent/NL7901916A/xx not_active Application Discontinuation
- 1979-03-09 DE DE19792909340 patent/DE2909340A1/de not_active Withdrawn
- 1979-03-09 SE SE7902140A patent/SE7902140L/ not_active Application Discontinuation
- 1979-03-09 DK DK98679A patent/DK98679A/da not_active Application Discontinuation
- 1979-03-09 BE BE0/193947A patent/BE874740A/xx not_active IP Right Cessation
- 1979-03-09 JP JP2680379A patent/JPS54129184A/ja active Pending
- 1979-03-09 CH CH228579A patent/CH640264A5/de not_active IP Right Cessation
- 1979-03-09 IT IT7948280A patent/IT7948280A0/it unknown
- 1979-08-08 IE IE598/79A patent/IE48321B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BE874740A (fr) | 1979-09-10 |
| IE790598L (en) | 1979-09-10 |
| DE2909340A1 (de) | 1979-09-13 |
| DK98679A (da) | 1979-09-11 |
| GB2016044B (en) | 1982-05-19 |
| SE7902140L (sv) | 1979-09-11 |
| FR2419321A1 (fr) | 1979-10-05 |
| NL7901916A (nl) | 1979-09-12 |
| GB2016044A (en) | 1979-09-19 |
| LU81018A1 (fr) | 1979-10-30 |
| JPS54129184A (en) | 1979-10-06 |
| CH640264A5 (de) | 1983-12-30 |
| FR2419321B1 (da) | 1984-02-24 |
| IT7948280A0 (it) | 1979-03-09 |
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