IL103603A - Proteins involved in the biosynthesis of a polypeptide antibiotic method for producing said protein and dna encoding them - Google Patents

Proteins involved in the biosynthesis of a polypeptide antibiotic method for producing said protein and dna encoding them

Info

Publication number: IL103603A
Authority: IL; Israel
Prior art keywords: epi; protein; dna; sequence; plasmid
Prior art date: 1991-10-31

Application number

IL10360392A

Other languages

English (en)

Other versions

IL103603A0 (en

Original Assignee

Thomae Gmbh Dr K

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-10-31

Filing date

1992-10-30

Publication date

1999-12-31

1992-10-30 Application filed by Thomae Gmbh Dr K filed Critical Thomae Gmbh Dr K

1993-03-15 Publication of IL103603A0 publication Critical patent/IL103603A0/xx

1999-12-31 Publication of IL103603A publication Critical patent/IL103603A/en

Links

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238000004811 liquid chromatography Methods 0.000 description 1
230000002934 lysing effect Effects 0.000 description 1
239000012139 lysis buffer Substances 0.000 description 1
VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
239000011976 maleic acid Substances 0.000 description 1
239000000463 material Substances 0.000 description 1
239000012528 membrane Substances 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
238000002156 mixing Methods 0.000 description 1
238000006011 modification reaction Methods 0.000 description 1
GNOLWGAJQVLBSM-UHFFFAOYSA-N n,n,5,7-tetramethyl-1,2,3,4-tetrahydronaphthalen-1-amine Chemical compound C1=C(C)C=C2C(N(C)C)CCCC2=C1C GNOLWGAJQVLBSM-UHFFFAOYSA-N 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
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239000002751 oligonucleotide probe Substances 0.000 description 1
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238000012261 overproduction Methods 0.000 description 1
XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 description 1
229960001723 oxytocin Drugs 0.000 description 1
238000012856 packing Methods 0.000 description 1
239000008188 pellet Substances 0.000 description 1
239000012071 phase Substances 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
150000008300 phosphoramidites Chemical class 0.000 description 1
238000000053 physical method Methods 0.000 description 1
230000004481 post-translational protein modification Effects 0.000 description 1
101150086745 pre gene Proteins 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
230000037452 priming Effects 0.000 description 1
235000019419 proteases Nutrition 0.000 description 1
230000017854 proteolysis Effects 0.000 description 1
210000001938 protoplast Anatomy 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
230000008707 rearrangement Effects 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
210000001995 reticulocyte Anatomy 0.000 description 1
238000010839 reverse transcription Methods 0.000 description 1
230000002441 reversible effect Effects 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
210000003705 ribosome Anatomy 0.000 description 1
JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 1
229960001225 rifampicin Drugs 0.000 description 1
108020000318 saccharopine dehydrogenase Proteins 0.000 description 1
235000019515 salmon Nutrition 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000012216 screening Methods 0.000 description 1
238000002741 site-directed mutagenesis Methods 0.000 description 1
FHHPUSMSKHSNKW-SMOYURAASA-M sodium deoxycholate Chemical compound [Na+].C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 FHHPUSMSKHSNKW-SMOYURAASA-M 0.000 description 1
238000010532 solid phase synthesis reaction Methods 0.000 description 1
241000894007 species Species 0.000 description 1
238000001228 spectrum Methods 0.000 description 1
230000002269 spontaneous effect Effects 0.000 description 1
239000007858 starting material Substances 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
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238000000856 sucrose gradient centrifugation Methods 0.000 description 1
239000011593 sulfur Substances 0.000 description 1
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230000004083 survival effect Effects 0.000 description 1
108010031354 thermitase Proteins 0.000 description 1
125000000101 thioether group Chemical group 0.000 description 1
125000003396 thiol group Chemical group [H]S* 0.000 description 1
229960004072 thrombin Drugs 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
229960000187 tissue plasminogen activator Drugs 0.000 description 1
VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
230000005030 transcription termination Effects 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
238000011426 transformation method Methods 0.000 description 1
230000005945 translocation Effects 0.000 description 1
239000001226 triphosphate Substances 0.000 description 1
235000011178 triphosphate Nutrition 0.000 description 1
125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
POSZUTFLHGNLHX-KSBRXOFISA-N tris maleate Chemical compound OCC(N)(CO)CO.OCC(N)(CO)CO.OC(=O)\C=C/C(O)=O POSZUTFLHGNLHX-KSBRXOFISA-N 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1
229960003726 vasopressin Drugs 0.000 description 1
238000005406 washing Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
- C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Gastroenterology & Hepatology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Cephalosporin Compounds (AREA)
Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
Compounds Of Unknown Constitution (AREA)
Epoxy Compounds (AREA)

IL10360392A 1991-10-31 1992-10-30 Proteins involved in the biosynthesis of a polypeptide antibiotic method for producing said protein and dna encoding them IL103603A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US78423491A	1991-10-31	1991-10-31

Publications (2)

Publication Number	Publication Date
IL103603A0 IL103603A0 (en)	1993-03-15
IL103603A true IL103603A (en)	1999-12-31

Family

ID=25131778

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL10360392A IL103603A (en)	1991-10-31	1992-10-30	Proteins involved in the biosynthesis of a polypeptide antibiotic method for producing said protein and dna encoding them

Country Status (18)

Country	Link
EP (1)	EP0543195B1 (de)
JP (1)	JP4117033B2 (de)
KR (1)	KR100242268B1 (de)
AT (1)	ATE162852T1 (de)
AU (1)	AU661054B2 (de)
CA (1)	CA2081704C (de)
CZ (2)	CZ286727B6 (de)
DE (1)	DE69224269T2 (de)
DK (1)	DK0543195T3 (de)
ES (1)	ES2112289T3 (de)
FI (1)	FI104498B (de)
GR (1)	GR3026465T3 (de)
HU (1)	HU215548B (de)
IL (1)	IL103603A (de)
MX (1)	MX9206265A (de)
NO (1)	NO307474B1 (de)
NZ (1)	NZ244976A (de)
ZA (1)	ZA928397B (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5639949A (en) *	1990-08-20	1997-06-17	Ciba-Geigy Corporation	Genes for the synthesis of antipathogenic substances
US5662898A (en) *	1990-08-20	1997-09-02	Ciba-Geigy Corporation	Genes for the synthesis of antipathogenic substances
US6117670A (en) *	1994-06-08	2000-09-12	Novartis Finance Corporation	Pyrrolnitrin biosynthesis genes and uses thereof
US6964760B2 (en)	1997-06-10	2005-11-15	University Of Florida Research Foundation	Antimicrobial polypeptide, nucleic acid, and methods of use
US6475771B1 (en)	1997-06-10	2002-11-05	University Of Florida Research Foundation	Antimicrobial polypeptides and methods of use
US5932469A (en)	1997-06-10	1999-08-03	University Of Florida	Antimicrobial polypeptide, nucleic acid, and methods of use
US6861236B2 (en)	2002-05-24	2005-03-01	Applied Nanosystems B.V.	Export and modification of (poly)peptides in the lantibiotic way
DE10261782A1 (de) *	2002-12-19	2004-07-01	Dr. Petry Genmedics Gmbh	Neue Gene und Genprodukte
DE602005009738D1 (de)	2004-12-07	2008-10-23	Applied Nanosystems Bv	Verfahren zur herstellung und sekretion modifizierter peptide
CN117511763A (zh) *	2022-08-05	2024-02-06	天康制药股份有限公司	一种表皮葡萄球菌高密度发酵培养基及其发酵方法和应用

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* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB8811761D0 (en) *	1988-05-18	1988-06-22	Thomae Gmbh Dr K	Biosynthetic process for preparation of chemical compounds

1992
- 1992-10-29 FI FI924909A patent/FI104498B/fi not_active IP Right Cessation
- 1992-10-29 KR KR1019920020041A patent/KR100242268B1/ko not_active Expired - Lifetime
- 1992-10-29 CA CA002081704A patent/CA2081704C/en not_active Expired - Lifetime
- 1992-10-30 AU AU27459/92A patent/AU661054B2/en not_active Expired
- 1992-10-30 ES ES92118598T patent/ES2112289T3/es not_active Expired - Lifetime
- 1992-10-30 DK DK92118598T patent/DK0543195T3/da active
- 1992-10-30 NO NO924192A patent/NO307474B1/no not_active IP Right Cessation
- 1992-10-30 IL IL10360392A patent/IL103603A/en not_active IP Right Cessation
- 1992-10-30 HU HU9203434A patent/HU215548B/hu unknown
- 1992-10-30 DE DE69224269T patent/DE69224269T2/de not_active Expired - Lifetime
- 1992-10-30 CZ CS19923269A patent/CZ286727B6/cs not_active IP Right Cessation
- 1992-10-30 CZ CZ963593A patent/CZ284861B6/cs not_active IP Right Cessation
- 1992-10-30 AT AT92118598T patent/ATE162852T1/de active
- 1992-10-30 MX MX9206265A patent/MX9206265A/es unknown
- 1992-10-30 EP EP92118598A patent/EP0543195B1/de not_active Expired - Lifetime
- 1992-10-30 ZA ZA928397A patent/ZA928397B/xx unknown
- 1992-11-02 NZ NZ244976A patent/NZ244976A/en not_active IP Right Cessation
- 1992-11-02 JP JP31553392A patent/JP4117033B2/ja not_active Expired - Lifetime
1998
- 1998-03-27 GR GR980400649T patent/GR3026465T3/el unknown

Also Published As

Publication number	Publication date
IL103603A0 (en)	1993-03-15
JPH05292952A (ja)	1993-11-09
EP0543195A3 (en)	1994-06-08
HUT65492A (en)	1994-06-28
CZ326992A3 (en)	1993-09-15
KR930008147A (ko)	1993-05-21
CZ359396A3 (cs)	1999-03-17
NO307474B1 (no)	2000-04-10
NO924192L (no)	1993-05-03
GR3026465T3 (en)	1998-06-30
EP0543195A2 (de)	1993-05-26
CA2081704A1 (en)	1993-05-01
CZ286727B6 (cs)	2000-06-14
FI104498B (fi)	2000-02-15
FI924909L (fi)	1993-05-01
MX9206265A (es)	1994-05-31
ES2112289T3 (es)	1998-04-01
HU9203434D0 (en)	1993-01-28
DK0543195T3 (da)	1998-09-23
EP0543195B1 (de)	1998-01-28
CA2081704C (en)	2007-05-01
AU661054B2 (en)	1995-07-13
AU2745992A (en)	1993-05-27
JP4117033B2 (ja)	2008-07-09
DE69224269D1 (de)	1998-03-05
NO924192D0 (no)	1992-10-30
HU215548B (hu)	1999-01-28
FI924909A0 (fi)	1992-10-29
KR100242268B1 (ko)	2000-02-01
ZA928397B (en)	1994-05-02
DE69224269T2 (de)	1998-06-04
ATE162852T1 (de)	1998-02-15
NZ244976A (en)	1995-07-26
CZ284861B6 (cs)	1999-03-17

Legal Events

Date	Code	Title
2000-06-29	FF	Patent granted
2003-01-12	KB	Patent renewed
2006-12-07	KB	Patent renewed
2013-05-30	EXP	Patent expired

Publication	Publication Date	Title
US5037760A (en)	1991-08-06	Secretory signal selection vectors for extracellular protein synthesis bacilli
US5631144A (en)	1997-05-20	Application of novel DNA fragments as a coding sequence for a signal peptide for the secretion of mature proteins by recombinant yeast, expression cassettes, transformed yeast and corresponding process for the preparation of proteins
Ottenwälder et al.	1995	Isolation and characterization of genetically engineered gallidermin and epidermin analogs
EP1507798A1 (de)	2005-02-23	Export und modifizierung von (poly)peptiden im lantibiotischen weg
CA2185343A1 (en)	1995-09-28	Enhanced secretion of polypeptides
IL190229A (en)	2012-12-31	Method for adding amide to their C-tip polypeptides with basic amino acid using specific endoproteases
US4801536A (en)	1989-01-31	Method for producing heterologous proteins
EP0543195B1 (de)	1998-01-28	Biosynthetisches Verfahren zur Herstellung von Protein
EP0546049B1 (de)	1995-07-19	Eine methode für die schnelle selektion von effizienten sekretionsvektoren
US5837485A (en)	1998-11-17	DNA encoding and biosynthetic process for the preparation of chemical compounds, lantibiotics
EP0342658B1 (de)	1994-05-11	Biosynthetisches Verfahren zur Herstellung von chemischen Verbindungen
JPH06501396A (ja)	1994-02-17	ストレプトミセスリビダンス由来の新規なプロテアーゼの単離及び特徴分析
US5962253A (en)	1999-10-05	Oxidative decarboxylation of peptides catalyzed by flavoprotein EpiD
EP0241546A1 (de)	1987-10-21	Verfahren zur herstellung heterologer proteine
SHIRozA et al.	2001	Construction of a chimeric shuttle plasmid via a heterodimer system: secretion of an scFv protein from Bacillus brevis cells capable of inhibiting hemagglutination
US4690898A (en)	1987-09-01	DNA coding for a signal peptide and DNA containing the same
Götz et al.	2001	Biosynthesis of the lantibiotics epidermin and gallidermin