IL107815A - Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing - Google Patents

Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing

Info

Publication number
IL107815A
IL107815A IL10781593A IL10781593A IL107815A IL 107815 A IL107815 A IL 107815A IL 10781593 A IL10781593 A IL 10781593A IL 10781593 A IL10781593 A IL 10781593A IL 107815 A IL107815 A IL 107815A
Authority
IL
Israel
Prior art keywords
stress
pct
methods
genetic constructs
responsive promoter
Prior art date
Application number
IL10781593A
Other languages
English (en)
Other versions
IL107815A0 (en
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26743124&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IL107815(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Du Pont filed Critical Du Pont
Publication of IL107815A0 publication Critical patent/IL107815A0/xx
Priority to IL13979400A priority Critical patent/IL139794A0/xx
Publication of IL107815A publication Critical patent/IL107815A/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
  • Enzymes And Modification Thereof (AREA)
IL10781593A 1992-12-04 1993-12-01 Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing IL107815A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
IL13979400A IL139794A0 (en) 1992-12-04 2000-11-20 A transformed bioluminescent microorganism comprising a stress-responsive promoter linked to a lux reporter operon and a tolc-mutation, which increases its bioluminescence upon environmental insult

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US98842892A 1992-12-04 1992-12-04
US6317393A 1993-05-14 1993-05-14

Publications (2)

Publication Number Publication Date
IL107815A0 IL107815A0 (en) 1994-04-12
IL107815A true IL107815A (en) 2003-12-10

Family

ID=26743124

Family Applications (2)

Application Number Title Priority Date Filing Date
IL10781593A IL107815A (en) 1992-12-04 1993-12-01 Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing
IL13979400A IL139794A0 (en) 1992-12-04 2000-11-20 A transformed bioluminescent microorganism comprising a stress-responsive promoter linked to a lux reporter operon and a tolc-mutation, which increases its bioluminescence upon environmental insult

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL13979400A IL139794A0 (en) 1992-12-04 2000-11-20 A transformed bioluminescent microorganism comprising a stress-responsive promoter linked to a lux reporter operon and a tolc-mutation, which increases its bioluminescence upon environmental insult

Country Status (11)

Country Link
US (1) US5683868A (de)
EP (1) EP0673439B2 (de)
JP (1) JPH08504101A (de)
KR (1) KR100262681B1 (de)
AT (1) ATE152181T1 (de)
AU (1) AU5730494A (de)
CA (1) CA2150232C (de)
DE (1) DE69310207T3 (de)
ES (1) ES2102811T5 (de)
IL (2) IL107815A (de)
WO (1) WO1994013831A1 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10509049A (ja) * 1994-11-23 1998-09-08 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 毒物検出のための凍結乾燥生物発光細菌試薬
WO1997041251A1 (en) * 1996-04-25 1997-11-06 Vlaamse Instelling Voor Technologisch Onderzoek (Vito) Recombinant nucleic acid sequences and methods for determining both genotoxicity and mutagenicity of a sample and the kinetics of the genetoxicity
US5817463A (en) * 1996-06-28 1998-10-06 Abbott Laboratories Nucleic acid primers and probes for detecting Mycoplasma pneumoniae
AUPO128096A0 (en) * 1996-07-29 1996-08-22 Crc For Waste Management And Pollution Control Limited Biosensors
US6025131A (en) * 1996-10-23 2000-02-15 E. I. Du Pont De Namours And Company Facile method for identifying regulated promoters
CA2267999A1 (en) * 1996-11-15 1998-05-22 E.I. Du Pont De Nemours And Company A small volume, highly sensitive method for detecting environmental insults
CA2279576A1 (en) * 1997-02-28 1998-09-03 E.I. Du Pont De Nemours And Company A method for identifying the site of action of xenobiotic chemicals
GB9713666D0 (en) * 1997-06-27 1997-09-03 Univ Cambridge Tech Biosensor materials and methods
JP2001516573A (ja) * 1997-09-18 2001-10-02 スミスクライン・ビーチャム・コーポレイション 抗微生物化合物のスクリーニング方法
IL137934A0 (en) * 1998-03-06 2001-10-31 Du Pont Methods for identifying polycationic, peptide-like compounds with antibacterial activity
US6703211B1 (en) * 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
EP0950717A1 (de) 1998-04-14 1999-10-20 "VLAAMSE INSTELLING VOOR TECHNOLOGISCH ONDERZOEK", afgekort "V.I.T.O." Diagnostisches System und Verfahren zur Bestimmung genotoxischer Substanzen in einer Probe
GB9819666D0 (en) * 1998-09-09 1998-11-04 Univ Aberdeen Biosensor
US6720415B2 (en) * 1998-12-02 2004-04-13 Princeton University Compositions and methods for regulating bacterial pathogenesis
US6602666B2 (en) 1998-12-02 2003-08-05 Vlaamse Instelling Technologisch Onderzoek (V.I.T.O.) Recombinant nucleic acid sequences and methods for determining both genotoxicity and mutagenicity of a sample and the kinetics of the genotoxicity
US6677431B2 (en) 1999-12-10 2004-01-13 The Trustees Of The University Of Pennsylvania Design, preparation, and properties of antibacterial β-peptides
GB0000908D0 (en) * 2000-01-14 2000-03-08 Scient Generics Ltd Parallel free-space optical communications
KR100414784B1 (ko) * 2000-01-18 2004-01-13 (주)바이오니아 연속식 수질 독성 검사 장치
KR100377539B1 (ko) * 2000-04-06 2003-03-26 광주과학기술원 식품내 잔류하는 농약에 대한 발광미생물을 이용한 독성탐지방법
US6716582B2 (en) * 2000-04-14 2004-04-06 E. I. Du Pont De Nemours And Company Cellular arrays for the identification of altered gene expression
KR20020041603A (ko) * 2000-11-28 2002-06-03 박호군 발광유전자 ｌｕｘＣＤＡＢＥ를 포함하는 재조합플라스미드, 이 플라스미드에 의해 형질 전환된 대장균ＳＢ０１(ＫＦＣＣ １１１７６) 및 이의 용도
CA2365791A1 (en) * 2000-12-22 2002-06-22 Pfizer Products Inc. Novel bioluminescent assays and bacterial strains useful therein
WO2002072789A2 (en) * 2001-03-12 2002-09-19 Irm, Llc. Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
DE10138766A1 (de) * 2001-08-07 2003-02-20 Bayer Cropscience Ag Verfahren zum Identifizieren von makrocyclischen Polyketiden
DE10242433A1 (de) * 2002-09-11 2004-03-25 Henkel Kgaa DNA-Chips zur Bioprozeßkontrolle
WO2005017198A2 (en) * 2003-08-18 2005-02-24 Aic Surface display expression system
WO2006018049A1 (en) * 2004-08-18 2006-02-23 Aic Surface display expression system
WO2006096063A1 (en) * 2005-03-11 2006-09-14 Stichting Voor De Technische Wetenschappen Construct and method for screening, selection and identification of stress factors
KR100697245B1 (ko) 2005-03-28 2007-03-21 한국생명공학연구원 중금속 유도성 프로모터와 이의 생물공학적 활용
WO2007058807A1 (en) * 2005-11-10 2007-05-24 Chaperone Technologies, Inc. Screening method for dnak inhibitors
KR100749493B1 (ko) 2006-03-22 2007-08-14 한국원자력연구원 대장균에서 ｒｐｏＳ 유전자의 발현을 조절함으로서프로피온산에 대한 미생물 감수성을 증진시키는 방법
RU2348687C1 (ru) * 2007-05-21 2009-03-10 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Штамм бактерий escherichia coli для тестирования присутствия в среде фенола и перекиси водорода
RU2355760C1 (ru) * 2007-10-29 2009-05-20 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП ГосНИИгенетика) НАБОР lux-БИОСЕНСОРОВ ДЛЯ ОПРЕДЕЛЕНИЯ ДЕТЕРГЕНТОВ ГИДРОФОБНОЙ ПРИРОДЫ В СРЕДЕ
US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
EP2748338B1 (de) * 2011-09-08 2017-04-26 Attagene, Inc. Systeme und verfahren für ein assay aus biokontaminantien in wasser
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria

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US4581335A (en) * 1982-12-01 1986-04-08 Texas A&M University System Process for producing a cloned luciferase-synthesizing microorganism
US5221623A (en) * 1986-07-22 1993-06-22 Boyce Thompson Institute For Plant Research, Inc. Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms
US5232833A (en) * 1988-09-14 1993-08-03 Stressgen Biotechnologies Corporation Accumulation of heat shock proteins for evaluating biological damage due to chronic exposure of an organism to sublethal levels of pollutants
DE3833628A1 (de) * 1988-10-03 1990-04-12 Genlux Forschungsgesellschaft Verfahren zum nachweis und zur identifikation toxischer substanzen mit hilfe klonierter mikroorganismen
DE3902982A1 (de) * 1989-02-01 1990-08-02 Genlux Forschungsgesellschaft Verfahren zum nachweis von quecksilber mit hilfe von durch quecksilber zu erhoehter biolumineszenz angeregter mikroorganismen
WO1992015687A1 (en) * 1991-03-01 1992-09-17 Vito Fused genes and their use for determining the presence of metals or of xenobiotic compounds
AU2422792A (en) * 1991-07-30 1993-03-02 Bio-Technical Resources Device for detecting aqueous contaminants
SG77101A1 (en) * 1992-07-06 2000-12-19 Harvard College Methods and diagnostic kits for determining toxicity utilizing bacterial stress promoters fused to reporter genes

Also Published As

Publication number Publication date
AU5730494A (en) 1994-07-04
IL107815A0 (en) 1994-04-12
EP0673439B1 (de) 1997-04-23
KR950704511A (ko) 1995-11-20
US5683868A (en) 1997-11-04
EP0673439B2 (de) 2001-07-04
DE69310207T2 (de) 1997-08-14
ES2102811T5 (es) 2001-10-16
WO1994013831A1 (en) 1994-06-23
KR100262681B1 (ko) 2000-08-01
CA2150232C (en) 2000-12-19
JPH08504101A (ja) 1996-05-07
ES2102811T3 (es) 1997-08-01
IL139794A0 (en) 2002-02-10
DE69310207T3 (de) 2001-11-15
ATE152181T1 (de) 1997-05-15
CA2150232A1 (en) 1994-06-23
DE69310207D1 (de) 1997-05-28
EP0673439A1 (de) 1995-09-27

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