IL108726A - Electrobiochemical method and system for the determination of an analyte which is a member of a recognition pair in a liquid medium and electrodes therefor - Google Patents

Electrobiochemical method and system for the determination of an analyte which is a member of a recognition pair in a liquid medium and electrodes therefor

Info

Publication number: IL108726A
Authority: IL; Israel
Prior art keywords: electrode; analyte; immobilized; state; concentration
Prior art date: 1994-02-22

Application number

IL10872694A

Other languages

English (en)

Other versions

IL108726A0 (en

Inventor

Itamar Willner

Arie Dagan

Shai Rubin

Ron Blonder

Azalia Riklin

Yael Cohen

Original Assignee

Yissum Res Dev Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1994-02-22

Filing date

1994-02-22

Publication date

1999-12-31

1994-02-22 Application filed by Yissum Res Dev Co filed Critical Yissum Res Dev Co

1994-02-22 Priority to IL10872694A priority Critical patent/IL108726A/en

1994-05-30 Publication of IL108726A0 publication Critical patent/IL108726A0/xx

1995-02-20 Priority to DE69526747T priority patent/DE69526747T2/de

1995-02-20 Priority to ES95102343T priority patent/ES2177586T3/es

1995-02-20 Priority to EP95102343A priority patent/EP0668502B1/en

1995-02-20 Priority to AT95102343T priority patent/ATE217966T1/de

1995-02-20 Priority to DK95102343T priority patent/DK0668502T3/da

1995-02-21 Priority to US08/390,978 priority patent/US5942388A/en

1995-02-22 Priority to JP05815895A priority patent/JP3583496B2/ja

1999-12-31 Publication of IL108726A publication Critical patent/IL108726A/en

Links

239000012491 analyte Substances 0.000 title claims abstract description 251
239000007788 liquid Substances 0.000 title claims abstract description 31
238000000034 method Methods 0.000 title claims description 29
230000004044 response Effects 0.000 claims abstract description 80
230000008859 change Effects 0.000 claims abstract description 48
230000015572 biosynthetic process Effects 0.000 claims abstract description 7
230000027455 binding Effects 0.000 claims description 72
239000003795 chemical substances by application Substances 0.000 claims description 50
239000000523 sample Substances 0.000 claims description 45
108090000790 Enzymes Proteins 0.000 claims description 35
102000004190 Enzymes Human genes 0.000 claims description 35
239000012472 biological sample Substances 0.000 claims description 23
239000007772 electrode material Substances 0.000 claims description 23
239000010931 gold Substances 0.000 claims description 18
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 17
229910052737 gold Inorganic materials 0.000 claims description 17
230000007423 decrease Effects 0.000 claims description 15
239000003153 chemical reaction reagent Substances 0.000 claims description 11
230000001965 increasing effect Effects 0.000 claims description 10
239000002243 precursor Substances 0.000 claims description 9
239000000126 substance Substances 0.000 claims description 9
238000004891 communication Methods 0.000 claims description 8
230000006854 communication Effects 0.000 claims description 8
239000011521 glass Substances 0.000 claims description 8
230000001939 inductive effect Effects 0.000 claims description 8
239000002523 lectin Substances 0.000 claims description 8
BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 8
239000000758 substrate Substances 0.000 claims description 6
238000007669 thermal treatment Methods 0.000 claims description 5
230000002829 reductive effect Effects 0.000 claims description 4
230000027756 respiratory electron transport chain Effects 0.000 claims description 4
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 3
229910052697 platinum Inorganic materials 0.000 claims description 3
229910052709 silver Inorganic materials 0.000 claims description 3
239000004332 silver Substances 0.000 claims description 3
230000003247 decreasing effect Effects 0.000 claims description 2
238000006479 redox reaction Methods 0.000 claims description 2
230000001678 irradiating effect Effects 0.000 claims 3
239000000243 solution Substances 0.000 description 61
239000000427 antigen Substances 0.000 description 40
108091007433 antigens Proteins 0.000 description 39
102000036639 antigens Human genes 0.000 description 39
239000010410 layer Substances 0.000 description 38
229940088598 enzyme Drugs 0.000 description 33
108010062580 Concanavalin A Proteins 0.000 description 20
238000006243 chemical reaction Methods 0.000 description 16
102000004169 proteins and genes Human genes 0.000 description 13
108090000623 proteins and genes Proteins 0.000 description 13
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 12
238000002484 cyclic voltammetry Methods 0.000 description 11
238000006317 isomerization reaction Methods 0.000 description 11
125000005647 linker group Chemical group 0.000 description 11
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
150000001875 compounds Chemical class 0.000 description 10
150000002148 esters Chemical class 0.000 description 10
238000011534 incubation Methods 0.000 description 9
108090000765 processed proteins & peptides Proteins 0.000 description 9
229910021607 Silver chloride Inorganic materials 0.000 description 8
OOTFVKOQINZBBF-UHFFFAOYSA-N cystamine Chemical compound CCSSCCN OOTFVKOQINZBBF-UHFFFAOYSA-N 0.000 description 8
HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 8
239000002356 single layer Substances 0.000 description 8
239000003792 electrolyte Substances 0.000 description 7
238000005259 measurement Methods 0.000 description 7
DZVCFNFOPIZQKX-LTHRDKTGSA-M merocyanine Chemical compound [Na+].O=C1N(CCCC)C(=O)N(CCCC)C(=O)C1=C\C=C\C=C/1N(CCCS([O-])(=O)=O)C2=CC=CC=C2O\1 DZVCFNFOPIZQKX-LTHRDKTGSA-M 0.000 description 7
229910052751 metal Inorganic materials 0.000 description 7
239000002184 metal Substances 0.000 description 7
230000004048 modification Effects 0.000 description 7
238000012986 modification Methods 0.000 description 7
NMHMNPHRMNGLLB-UHFFFAOYSA-N phloretic acid Chemical group OC(=O)CCC1=CC=C(O)C=C1 NMHMNPHRMNGLLB-UHFFFAOYSA-N 0.000 description 7
230000002441 reversible effect Effects 0.000 description 7
230000035945 sensitivity Effects 0.000 description 7
238000011088 calibration curve Methods 0.000 description 6
229940099500 cystamine Drugs 0.000 description 6
150000002772 monosaccharides Chemical class 0.000 description 6
239000008055 phosphate buffer solution Substances 0.000 description 6
CNJJDQUUYRTYHA-UHFFFAOYSA-N C[N+](C=C1)=CC=C1C(C=C1)=CC[N+]1=CC([O-])=O Chemical compound C[N+](C=C1)=CC=C1C(C=C1)=CC[N+]1=CC([O-])=O CNJJDQUUYRTYHA-UHFFFAOYSA-N 0.000 description 5
NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 5
239000007864 aqueous solution Substances 0.000 description 5
238000002848 electrochemical method Methods 0.000 description 5
238000002474 experimental method Methods 0.000 description 5
238000003018 immunoassay Methods 0.000 description 5
238000002360 preparation method Methods 0.000 description 5
230000009467 reduction Effects 0.000 description 5
239000011780 sodium chloride Substances 0.000 description 5
HBOMLICNUCNMMY-KJFJCRTCSA-N 1-[(4s,5s)-4-azido-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical class O=C1NC(=O)C(C)=CN1C1O[C@H](CO)[C@@H](N=[N+]=[N-])C1 HBOMLICNUCNMMY-KJFJCRTCSA-N 0.000 description 4
CSPTZWQFHBVOLO-UHFFFAOYSA-N 4-phenyldiazenylbenzoic acid Chemical compound C1=CC(C(=O)O)=CC=C1N=NC1=CC=CC=C1 CSPTZWQFHBVOLO-UHFFFAOYSA-N 0.000 description 4
WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 4
239000004472 Lysine Substances 0.000 description 4
XBDQKXXYIPTUBI-UHFFFAOYSA-N Propionic acid Chemical compound CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 4
239000005864 Sulphur Substances 0.000 description 4
230000001419 dependent effect Effects 0.000 description 4
239000012153 distilled water Substances 0.000 description 4
125000000524 functional group Chemical group 0.000 description 4
239000000203 mixture Substances 0.000 description 4
239000008363 phosphate buffer Substances 0.000 description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
YCLSOMLVSHPPFV-UHFFFAOYSA-N 3-(2-carboxyethyldisulfanyl)propanoic acid Chemical compound OC(=O)CCSSCCC(O)=O YCLSOMLVSHPPFV-UHFFFAOYSA-N 0.000 description 3
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
-1 CI or OH Chemical class 0.000 description 3
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
150000001412 amines Chemical group 0.000 description 3
125000003277 amino group Chemical group 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
230000000890 antigenic effect Effects 0.000 description 3
239000001110 calcium chloride Substances 0.000 description 3
235000011148 calcium chloride Nutrition 0.000 description 3
229910001628 calcium chloride Inorganic materials 0.000 description 3
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
230000008878 coupling Effects 0.000 description 3
238000010168 coupling process Methods 0.000 description 3
238000005859 coupling reaction Methods 0.000 description 3
YAGKRVSRTSUGEY-UHFFFAOYSA-N ferricyanide Chemical compound [Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YAGKRVSRTSUGEY-UHFFFAOYSA-N 0.000 description 3
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
230000003100 immobilizing effect Effects 0.000 description 3
AMGQUBHHOARCQH-UHFFFAOYSA-N indium;oxotin Chemical compound [In].[Sn]=O AMGQUBHHOARCQH-UHFFFAOYSA-N 0.000 description 3
238000011068 loading method Methods 0.000 description 3
150000002739 metals Chemical class 0.000 description 3
108091005573 modified proteins Proteins 0.000 description 3
102000035118 modified proteins Human genes 0.000 description 3
239000000047 product Substances 0.000 description 3
WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 2
108090001090 Lectins Proteins 0.000 description 2
102000004856 Lectins Human genes 0.000 description 2
229910021380 Manganese Chloride Inorganic materials 0.000 description 2
GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 2
NQRYJNQNLNOLGT-UHFFFAOYSA-N Piperidine Chemical compound C1CCNCC1 NQRYJNQNLNOLGT-UHFFFAOYSA-N 0.000 description 2
125000002252 acyl group Chemical group 0.000 description 2
125000003342 alkenyl group Chemical group 0.000 description 2
125000004450 alkenylene group Chemical group 0.000 description 2
125000002947 alkylene group Chemical group 0.000 description 2
238000003556 assay Methods 0.000 description 2
239000000872 buffer Substances 0.000 description 2
150000001718 carbodiimides Chemical class 0.000 description 2
239000007795 chemical reaction product Substances 0.000 description 2
238000010276 construction Methods 0.000 description 2
XVOYSCVBGLVSOL-UHFFFAOYSA-N cysteic acid Chemical compound OC(=O)C(N)CS(O)(=O)=O XVOYSCVBGLVSOL-UHFFFAOYSA-N 0.000 description 2
238000001514 detection method Methods 0.000 description 2
230000000694 effects Effects 0.000 description 2
238000006911 enzymatic reaction Methods 0.000 description 2
150000004820 halides Chemical group 0.000 description 2
229910052736 halogen Inorganic materials 0.000 description 2
150000002367 halogens Chemical class 0.000 description 2
238000005286 illumination Methods 0.000 description 2
238000009413 insulation Methods 0.000 description 2
JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
150000002605 large molecules Chemical class 0.000 description 2
125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 2
229920002521 macromolecule Polymers 0.000 description 2
FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 2
229940107698 malachite green Drugs 0.000 description 2
239000011565 manganese chloride Substances 0.000 description 2
235000002867 manganese chloride Nutrition 0.000 description 2
238000004519 manufacturing process Methods 0.000 description 2
125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
238000007699 photoisomerization reaction Methods 0.000 description 2
229920001184 polypeptide Polymers 0.000 description 2
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RPENMORRBUTCPR-UHFFFAOYSA-M sodium;1-hydroxy-2,5-dioxopyrrolidine-3-sulfonate Chemical compound [Na+].ON1C(=O)CC(S([O-])(=O)=O)C1=O RPENMORRBUTCPR-UHFFFAOYSA-M 0.000 description 2
125000003003 spiro group Chemical group 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
238000012360 testing method Methods 0.000 description 2
UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 2
238000001075 voltammogram Methods 0.000 description 2
LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical compound CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 1
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
ZXVONLUNISGICL-UHFFFAOYSA-N 4,6-dinitro-o-cresol Chemical group CC1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O ZXVONLUNISGICL-UHFFFAOYSA-N 0.000 description 1
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244000125300 Argania sideroxylon Species 0.000 description 1
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
108090000317 Chymotrypsin Proteins 0.000 description 1
RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
239000007995 HEPES buffer Substances 0.000 description 1
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239000004365 Protease Substances 0.000 description 1
ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
241000700605 Viruses Species 0.000 description 1
238000011481 absorbance measurement Methods 0.000 description 1
239000002253 acid Substances 0.000 description 1
125000000217 alkyl group Chemical group 0.000 description 1
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WQZGKKKJIJFFOK-PQMKYFCFSA-N alpha-D-mannose Chemical compound OC[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-PQMKYFCFSA-N 0.000 description 1
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239000012298 atmosphere Substances 0.000 description 1
JXLHNMVSKXFWAO-UHFFFAOYSA-N azane;7-fluoro-2,1,3-benzoxadiazole-4-sulfonic acid Chemical compound N.OS(=O)(=O)C1=CC=C(F)C2=NON=C12 JXLHNMVSKXFWAO-UHFFFAOYSA-N 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
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125000004432 carbon atom Chemical group C* 0.000 description 1
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230000015556 catabolic process Effects 0.000 description 1
OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
229960001231 choline Drugs 0.000 description 1
229960002376 chymotrypsin Drugs 0.000 description 1
230000009137 competitive binding Effects 0.000 description 1
230000009918 complex formation Effects 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
229910052802 copper Inorganic materials 0.000 description 1
239000010949 copper Substances 0.000 description 1
125000004122 cyclic group Chemical group 0.000 description 1
UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
238000011161 development Methods 0.000 description 1
230000018109 developmental process Effects 0.000 description 1
PQZTVWVYCLIIJY-UHFFFAOYSA-N diethyl(propyl)amine Chemical compound CCCN(CC)CC PQZTVWVYCLIIJY-UHFFFAOYSA-N 0.000 description 1
238000010494 dissociation reaction Methods 0.000 description 1
230000005593 dissociations Effects 0.000 description 1
QFTYSVGGYOXFRQ-UHFFFAOYSA-N dodecane-1,12-diamine Chemical compound NCCCCCCCCCCCCN QFTYSVGGYOXFRQ-UHFFFAOYSA-N 0.000 description 1
238000001035 drying Methods 0.000 description 1
238000000835 electrochemical detection Methods 0.000 description 1
238000003891 environmental analysis Methods 0.000 description 1
125000004185 ester group Chemical group 0.000 description 1
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
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239000004310 lactic acid Substances 0.000 description 1
235000014655 lactic acid Nutrition 0.000 description 1
239000003446 ligand Substances 0.000 description 1
238000012886 linear function Methods 0.000 description 1
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239000002207 metabolite Substances 0.000 description 1
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239000012487 rinsing solution Substances 0.000 description 1
238000002444 silanisation Methods 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
NGDIAZZSCVVCEW-UHFFFAOYSA-M sodium;butyl sulfate Chemical compound [Na+].CCCCOS([O-])(=O)=O NGDIAZZSCVVCEW-UHFFFAOYSA-M 0.000 description 1
239000007790 solid phase Substances 0.000 description 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/004—Enzyme electrodes mediator-assisted
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/002—Electrode membranes
- C12Q1/003—Functionalisation
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/552—Glass or silica
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/553—Metal or metal coated
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/806—Electrical property or magnetic property
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/825—Pretreatment for removal of interfering factors from sample

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IL10872694A 1994-02-22 1994-02-22 Electrobiochemical method and system for the determination of an analyte which is a member of a recognition pair in a liquid medium and electrodes therefor IL108726A (en)

Priority Applications (8)

Application Number	Priority Date	Filing Date	Title
IL10872694A IL108726A (en)	1994-02-22	1994-02-22	Electrobiochemical method and system for the determination of an analyte which is a member of a recognition pair in a liquid medium and electrodes therefor
DE69526747T DE69526747T2 (de)	1994-02-22	1995-02-20	Elektrobiochemisches Verfahren, System und Elektroden zur Bestimmung eines Mitgliedes eines Bindungspaares
ES95102343T ES2177586T3 (es)	1994-02-22	1995-02-20	Un procedimiento electrobioquimico, sistema y electrodos para determinar un miembro de un par de reconocimiento.
EP95102343A EP0668502B1 (en)	1994-02-22	1995-02-20	Electrobiochemical method, system and electrodes for determination of a member of a recognition pair
AT95102343T ATE217966T1 (de)	1994-02-22	1995-02-20	Elektrobiochemisches verfahren, system und elektroden zur bestimmung eines mitgliedes eines bindungspaares
DK95102343T DK0668502T3 (da)	1994-02-22	1995-02-20	Elektrobiokemisk fremgangsmåde, system og elektroder til bestemmelse af en del af et bindingspar
US08/390,978 US5942388A (en)	1994-02-22	1995-02-21	Electrobiochemical method and system for the determination of an analyte which is a member of a recognition pair in a liquid medium, and electrodes thereof
JP05815895A JP3583496B2 (ja)	1994-02-22	1995-02-22	液体媒質中の認識対の構成員である被検体の決定のための電気生化学的方法および系ならびにその電極

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
IL10872694A IL108726A (en)	1994-02-22	1994-02-22	Electrobiochemical method and system for the determination of an analyte which is a member of a recognition pair in a liquid medium and electrodes therefor

Publications (2)

Publication Number	Publication Date
IL108726A0 IL108726A0 (en)	1994-05-30
IL108726A true IL108726A (en)	1999-12-31

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Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL10872694A IL108726A (en)	1994-02-22	1994-02-22	Electrobiochemical method and system for the determination of an analyte which is a member of a recognition pair in a liquid medium and electrodes therefor

Country Status (8)

Country	Link
US (1)	US5942388A (es)
EP (1)	EP0668502B1 (es)
JP (1)	JP3583496B2 (es)
AT (1)	ATE217966T1 (es)
DE (1)	DE69526747T2 (es)
DK (1)	DK0668502T3 (es)
ES (1)	ES2177586T3 (es)
IL (1)	IL108726A (es)

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1994
- 1994-02-22 IL IL10872694A patent/IL108726A/en not_active IP Right Cessation
1995
- 1995-02-20 DK DK95102343T patent/DK0668502T3/da active
- 1995-02-20 AT AT95102343T patent/ATE217966T1/de not_active IP Right Cessation
- 1995-02-20 DE DE69526747T patent/DE69526747T2/de not_active Expired - Fee Related
- 1995-02-20 EP EP95102343A patent/EP0668502B1/en not_active Expired - Lifetime
- 1995-02-20 ES ES95102343T patent/ES2177586T3/es not_active Expired - Lifetime
- 1995-02-21 US US08/390,978 patent/US5942388A/en not_active Expired - Fee Related
- 1995-02-22 JP JP05815895A patent/JP3583496B2/ja not_active Expired - Fee Related

Also Published As

Publication number	Publication date
IL108726A0 (en)	1994-05-30
EP0668502B1 (en)	2002-05-22
EP0668502A2 (en)	1995-08-23
EP0668502A3 (en)	1996-02-28
DE69526747T2 (de)	2002-10-10
JPH07301615A (ja)	1995-11-14
DE69526747D1 (de)	2002-06-27
ATE217966T1 (de)	2002-06-15
JP3583496B2 (ja)	2004-11-04
US5942388A (en)	1999-08-24
DK0668502T3 (da)	2002-08-19
ES2177586T3 (es)	2002-12-16

Legal Events

Date	Code	Title
2000-06-29	FF	Patent granted
2000-10-31	KB	Patent renewed
2002-09-12	HP	Change in proprietorship
2004-06-01	KB	Patent renewed
2008-03-23	KB	Patent renewed
2012-10-31	MM9K	Patent not in force due to non-payment of renewal fees

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