IL111996A - Enzymatic conversion of proteins using immobilized dictyostelium dipeptidylaminopeptidase - Google Patents

Enzymatic conversion of proteins using immobilized dictyostelium dipeptidylaminopeptidase

Info

Publication number
IL111996A
IL111996A IL11199694A IL11199694A IL111996A IL 111996 A IL111996 A IL 111996A IL 11199694 A IL11199694 A IL 11199694A IL 11199694 A IL11199694 A IL 11199694A IL 111996 A IL111996 A IL 111996A
Authority
IL
Israel
Prior art keywords
met
ddap
precursor
human proinsulin
human
Prior art date
Application number
IL11199694A
Other languages
English (en)
Other versions
IL111996A0 (en
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Publication of IL111996A0 publication Critical patent/IL111996A0/xx
Publication of IL111996A publication Critical patent/IL111996A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/14—Dipeptidyl-peptidases and tripeptidyl-peptidases (3.4.14)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
IL11199694A 1993-12-22 1994-12-15 Enzymatic conversion of proteins using immobilized dictyostelium dipeptidylaminopeptidase IL111996A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/172,501 US5573923A (en) 1993-12-22 1993-12-22 Method for removing N-terminal dipeptides from precursor polypeptides with immobilized dipeptidylaminopeptidase from dictyostelium discoideum

Publications (2)

Publication Number Publication Date
IL111996A0 IL111996A0 (en) 1995-03-15
IL111996A true IL111996A (en) 1998-12-06

Family

ID=22627963

Family Applications (1)

Application Number Title Priority Date Filing Date
IL11199694A IL111996A (en) 1993-12-22 1994-12-15 Enzymatic conversion of proteins using immobilized dictyostelium dipeptidylaminopeptidase

Country Status (11)

Country Link
US (1) US5573923A (de)
EP (1) EP0659886B1 (de)
JP (1) JPH07203986A (de)
KR (1) KR950018481A (de)
AT (1) ATE220108T1 (de)
BR (1) BR9405145A (de)
CA (1) CA2138099A1 (de)
DE (1) DE69430898T2 (de)
FI (1) FI945977L (de)
HU (1) HU219544B (de)
IL (1) IL111996A (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5840517A (en) * 1995-04-26 1998-11-24 Eli Lilly And Company Process for preparing obesity protein analogs
US5614379A (en) * 1995-04-26 1997-03-25 Eli Lilly And Company Process for preparing anti-obesity protein
US6743600B1 (en) 1999-04-30 2004-06-01 Monsanto Technologies Llc Method of removing N-terminal alanine residues from polypeptides with Aeromonas aminopeptidase
US6794159B1 (en) 1999-04-30 2004-09-21 Pharmacia Corporation Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase
HU225243B1 (en) * 2001-09-13 2006-08-28 Genentech Inc Gram-negative bacterium cell containing a gene coding an aminopeptidase
SE0201738D0 (sv) * 2002-06-07 2002-06-07 Aamic Ab Micro-fluid structures
WO2011103325A1 (en) * 2010-02-17 2011-08-25 Elona Biotechnologies Methods for preparing human growth hormone

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK55685A (da) * 1985-02-07 1986-08-08 Nordisk Gentofte Enzym eller enzymkompleks med proteolytisk aktivitet
KR900702014A (ko) * 1988-09-13 1990-12-05 원본미기재 아미노펩티다아제 ap1 및 ap122의 단리, 정제 및 특성확인
US5126249A (en) * 1989-05-09 1992-06-30 Eli Lilly And Company Enzymatic removal of a protein amino-terminal sequence
CA2089541A1 (en) * 1992-02-18 1993-08-19 Bruce H. Frank Selective removal of n-terminal extensions from folded insulin precursors using dipeptidyl-aminopeptidase-1
TW257792B (de) * 1992-10-01 1995-09-21 Lilly Co Eli

Also Published As

Publication number Publication date
HU9403696D0 (en) 1995-02-28
EP0659886A1 (de) 1995-06-28
EP0659886B1 (de) 2002-07-03
CA2138099A1 (en) 1995-06-23
KR950018481A (ko) 1995-07-22
DE69430898T2 (de) 2003-01-23
FI945977A0 (fi) 1994-12-20
US5573923A (en) 1996-11-12
FI945977A7 (fi) 1995-06-23
FI945977L (fi) 1995-06-23
HU219544B (hu) 2001-05-28
DE69430898D1 (de) 2002-08-08
JPH07203986A (ja) 1995-08-08
BR9405145A (pt) 1995-10-17
ATE220108T1 (de) 2002-07-15
HUT69957A (en) 1995-09-28
IL111996A0 (en) 1995-03-15

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Legal Events

Date Code Title Description
FF Patent granted
KB Patent renewed
KB Patent renewed
MM9K Patent not in force due to non-payment of renewal fees