ATE220108T1 - Enzymatische umsetzung von proteinen unter verwendung immobilisierter dipeptidylaminopeptidase aus dictyostelium - Google Patents

Enzymatische umsetzung von proteinen unter verwendung immobilisierter dipeptidylaminopeptidase aus dictyostelium

Info

Publication number
ATE220108T1
ATE220108T1 AT94309313T AT94309313T ATE220108T1 AT E220108 T1 ATE220108 T1 AT E220108T1 AT 94309313 T AT94309313 T AT 94309313T AT 94309313 T AT94309313 T AT 94309313T AT E220108 T1 ATE220108 T1 AT E220108T1
Authority
AT
Austria
Prior art keywords
ddap
proteins
human proinsulin
dipeptidylaminopeptidase
kpb
Prior art date
Application number
AT94309313T
Other languages
English (en)
Inventor
Paul Robert Atkinson
Lisa Kay Foster
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Application granted granted Critical
Publication of ATE220108T1 publication Critical patent/ATE220108T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/62Insulins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/18Ion-exchange chromatography
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/14Dipeptidyl-peptidases and tripeptidyl-peptidases (3.4.14)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
AT94309313T 1993-12-22 1994-12-14 Enzymatische umsetzung von proteinen unter verwendung immobilisierter dipeptidylaminopeptidase aus dictyostelium ATE220108T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/172,501 US5573923A (en) 1993-12-22 1993-12-22 Method for removing N-terminal dipeptides from precursor polypeptides with immobilized dipeptidylaminopeptidase from dictyostelium discoideum

Publications (1)

Publication Number Publication Date
ATE220108T1 true ATE220108T1 (de) 2002-07-15

Family

ID=22627963

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94309313T ATE220108T1 (de) 1993-12-22 1994-12-14 Enzymatische umsetzung von proteinen unter verwendung immobilisierter dipeptidylaminopeptidase aus dictyostelium

Country Status (11)

Country Link
US (1) US5573923A (de)
EP (1) EP0659886B1 (de)
JP (1) JPH07203986A (de)
KR (1) KR950018481A (de)
AT (1) ATE220108T1 (de)
BR (1) BR9405145A (de)
CA (1) CA2138099A1 (de)
DE (1) DE69430898T2 (de)
FI (1) FI945977L (de)
HU (1) HU219544B (de)
IL (1) IL111996A (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5840517A (en) * 1995-04-26 1998-11-24 Eli Lilly And Company Process for preparing obesity protein analogs
US5614379A (en) * 1995-04-26 1997-03-25 Eli Lilly And Company Process for preparing anti-obesity protein
US6743600B1 (en) 1999-04-30 2004-06-01 Monsanto Technologies Llc Method of removing N-terminal alanine residues from polypeptides with Aeromonas aminopeptidase
US6794159B1 (en) 1999-04-30 2004-09-21 Pharmacia Corporation Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase
HU225243B1 (en) * 2001-09-13 2006-08-28 Genentech Inc Gram-negative bacterium cell containing a gene coding an aminopeptidase
SE0201738D0 (sv) * 2002-06-07 2002-06-07 Aamic Ab Micro-fluid structures
WO2011103325A1 (en) * 2010-02-17 2011-08-25 Elona Biotechnologies Methods for preparing human growth hormone

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK55685A (da) * 1985-02-07 1986-08-08 Nordisk Gentofte Enzym eller enzymkompleks med proteolytisk aktivitet
KR900702014A (ko) * 1988-09-13 1990-12-05 원본미기재 아미노펩티다아제 ap1 및 ap122의 단리, 정제 및 특성확인
US5126249A (en) * 1989-05-09 1992-06-30 Eli Lilly And Company Enzymatic removal of a protein amino-terminal sequence
CA2089541A1 (en) * 1992-02-18 1993-08-19 Bruce H. Frank Selective removal of n-terminal extensions from folded insulin precursors using dipeptidyl-aminopeptidase-1
TW257792B (de) * 1992-10-01 1995-09-21 Lilly Co Eli

Also Published As

Publication number Publication date
HU9403696D0 (en) 1995-02-28
IL111996A (en) 1998-12-06
EP0659886A1 (de) 1995-06-28
EP0659886B1 (de) 2002-07-03
CA2138099A1 (en) 1995-06-23
KR950018481A (ko) 1995-07-22
DE69430898T2 (de) 2003-01-23
FI945977A0 (fi) 1994-12-20
US5573923A (en) 1996-11-12
FI945977A7 (fi) 1995-06-23
FI945977L (fi) 1995-06-23
HU219544B (hu) 2001-05-28
DE69430898D1 (de) 2002-08-08
JPH07203986A (ja) 1995-08-08
BR9405145A (pt) 1995-10-17
HUT69957A (en) 1995-09-28
IL111996A0 (en) 1995-03-15

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee