IL77362A - Hygromycin B resistance given by VIHPA gene as a selectable marker in plants - Google Patents

Hygromycin B resistance given by VIHPA gene as a selectable marker in plants

Info

Publication number: IL77362A
Authority: IL; Israel
Prior art keywords: hygromycin; plant; cell; gene; plasmid
Prior art date: 1984-12-24

Application number

IL7736285A

Other languages

English (en)

Hebrew (he)

Original Assignee

Lilly Co Eli

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-12-24

Filing date

1985-12-17

Publication date

1994-01-25

1985-12-17 Application filed by Lilly Co Eli filed Critical Lilly Co Eli

1994-01-25 Publication of IL77362A publication Critical patent/IL77362A/en

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

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Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Cell Biology (AREA)
Medicinal Chemistry (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

IL7736285A 1984-12-24 1985-12-17 Hygromycin B resistance given by VIHPA gene as a selectable marker in plants IL77362A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US68582484A	1984-12-24	1984-12-24

Publications (1)

Publication Number	Publication Date
IL77362A true IL77362A (en)	1994-01-25

Family

ID=24753822

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL7736285A IL77362A (en)	1984-12-24	1985-12-17	Hygromycin B resistance given by VIHPA gene as a selectable marker in plants

Country Status (7)

Country	Link
US (2)	US6048730A (fr)
EP (1)	EP0186425B1 (fr)
JP (2)	JP3090926B2 (fr)
CA (1)	CA1340766C (fr)
DE (1)	DE3586716T2 (fr)
DK (1)	DK175506B1 (fr)
IL (1)	IL77362A (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0223417A1 (fr) *	1985-10-21	1987-05-27	Lubrizol Genetics Inc.	Plasmides sous-T-ADN à base de TL-ADN
EP0265556A1 (fr) *	1986-10-31	1988-05-04	Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.	Vecteurs binaires stables pour l'agrobactérium et leur utilisation
US5407454A (en) *	1989-11-07	1995-04-18	Pioneer Hi-Bred International, Inc.	Larvicidal lectins and plant insect resistance based thereon
CA2061862A1 (fr) *	1991-03-04	1992-09-05	Jonathan Duvick	Proteines naturelles et synthetiques ayant une activite inhibitrice vis-a-vis des microorganismes pathogenes
US7067719B1 (en) *	1999-05-05	2006-06-27	Seminis Vegetable Seeds, Inc.	Transformation of Allium sp. with Agrobacterium using embryogenic callus cultures
WO2001059086A2 (fr) *	2000-02-08	2001-08-16	Sakata Seed Corporation	Nouveaux procedes et nouvelles constructions permettant la transformation de vegetaux
US7188298B2 (en) *	2001-03-14	2007-03-06	Matsushita Electric Industrial Co., Ltd.	Error correction encoding and decoding methods and apparatuses for DVI audio data
EP1279737A1 (fr)	2001-07-27	2003-01-29	Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A.	Methode de transformation pour la génération des plants sans marqueurs selectionable
US7329798B2 (en) *	2002-06-28	2008-02-12	University Of Guelph	Harvest-inducible regulatory elements and methods of using same
CA2490781A1 (fr)	2002-06-28	2004-01-08	University Of Guelph	Nouveaux genes inductibles issus de la luzerne et procede d'utilisation de ces genes
DK1521603T3 (da) *	2002-07-12	2011-04-18	Cook Inc	Coated medicinsk anordning
KR100769584B1 (ko) *	2004-07-30	2007-10-23	학교법인 포항공과대학교	셀룰로오스의 자가가수분해를 위한 셀룰라아제 발현형질전환 식물체 및 이를 이용한 수용성 당의 생산방법
EP1949785A1 (fr)	2007-01-26	2008-07-30	Coöperatie AVEBE U.A.	Utilisation de gènes R pour marquer une sélection dans la transformation de plantes et utilisation de cisgènes pour la transformation de plantes
CN102666572B (zh)	2009-09-18	2016-08-10	瓦赫宁恩大学	来自Solanum chacoense的功能性R-基因的克隆和开发
EP2390332A1 (fr)	2010-05-31	2011-11-30	Coöperatie Avebe U.A.	Clonage et exploitation d'un gène R fonctionnel de Solanum x edinense
NL2006378C2 (en)	2011-03-11	2012-09-12	Univ Wageningen	Tyclv resistance.
EP2855682A1 (fr)	2012-05-25	2015-04-08	Wageningen Universiteit	Nouveau gène de résistance pour plantes
WO2014112875A1 (fr)	2013-01-17	2014-07-24	Wageningen Universiteit	Nouveau procédé pour conférer aux plantes une résistance à la pourriture molle bactérienne
NL2014107B1 (en)	2015-01-09	2016-09-29	Limgroup B V	New methods and products for breeding of asparagus.

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL66065A (en) *	1981-06-22	1989-06-30	Lilly Co Eli	Recombinant dna cloning vectors and the e.coli transformants thereof
US4727028A (en) *	1981-06-22	1988-02-23	Eli Lilly And Company	Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof
JPS5813598A (ja) *	1981-06-22	1983-01-26	イーライ・リリー・アンド・カンパニー	組み替えｄｎａクロ−ニング・ベクタ−ならびにその真核細胞性および原核細胞性被形質転換体
US4536475A (en) *	1982-10-05	1985-08-20	Phytogen	Plant vector
EP0131623B2 (fr) *	1983-01-17	1999-07-28	Monsanto Company	Genes chimeriques appropries a l'expression dans des cellules vegetales
US4960704A (en) *	1983-07-22	1990-10-02	Eli Lilly And Company	Modified antibiotic resistance gene
CA1278540C (fr) *	1983-07-22	1991-01-02	Eli Lilly And Company	Gene modifie conferant une resistance a un antibiotique
NZ209338A (en) *	1983-09-14	1988-02-12	Lubrizol Genetics Inc	Plasmid for the transformation of a plant cell
DE3343243A1 (de) *	1983-11-30	1985-06-05	Robert 8183 Rottach-Egern Neumeier	Kindersichere schaltuhr fuer fernsehgeraete o.dgl.
WO1986003516A1 (fr)	1984-12-13	1986-06-19	Biotechnica International, Inc.	Vecteur de transformation vegetale
US5668298A (en) *	1984-12-24	1997-09-16	Eli Lilly And Company	Selectable marker for development of vectors and transformation systems in plants
CA1341455C (fr) *	1986-06-30	2004-04-27	Stephen L. Goldman	Procede de transformation de graminees, ainsi que les produits qui en resultent
EP0267159A3 (fr) *	1986-11-07	1990-05-02	Ciba-Geigy Ag	Procédé de modification génétique de monocotylédones
EP0289478A3 (fr) *	1987-04-27	1990-10-24	Monsanto Company	Gènes marqueurs de gentamicine pour la transformation de plantes
EP0298478A3 (fr)	1987-07-10	1990-01-17	Ciba-Geigy Ag	Procédé pour la fabrication d'alpha-fluorcétones
CN1087735A (zh) *	1992-07-21	1994-06-08	太阳线集团有限公司	带有电子设备的装置

1985
- 1985-12-16 CA CA000497740A patent/CA1340766C/fr not_active Expired - Fee Related
- 1985-12-17 IL IL7736285A patent/IL77362A/en not_active IP Right Cessation
- 1985-12-18 EP EP85309217A patent/EP0186425B1/fr not_active Expired
- 1985-12-18 DK DK198505890A patent/DK175506B1/da not_active IP Right Cessation
- 1985-12-18 DE DE8585309217T patent/DE3586716T2/de not_active Expired - Lifetime
- 1985-12-20 JP JP60289086A patent/JP3090926B2/ja not_active Expired - Lifetime
1990
- 1990-09-19 US US07/586,317 patent/US6048730A/en not_active Expired - Lifetime
1995
- 1995-12-28 JP JP7342648A patent/JP2815837B2/ja not_active Expired - Lifetime
2000
- 2000-02-09 US US09/500,502 patent/US6365799B1/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
JPH08224085A (ja)	1996-09-03
JPS61162188A (ja)	1986-07-22
EP0186425B1 (fr)	1992-09-30
EP0186425A3 (en)	1987-01-14
DK175506B1 (da)	2004-11-15
JP2815837B2 (ja)	1998-10-27
DK589085D0 (da)	1985-12-18
DE3586716T2 (de)	1993-03-11
EP0186425A2 (fr)	1986-07-02
US6365799B1 (en)	2002-04-02
US6048730A (en)	2000-04-11
JP3090926B2 (ja)	2000-09-25
CA1340766C (fr)	1999-09-28
DK589085A (da)	1986-06-25
DE3586716D1 (de)	1992-11-05

Legal Events

Date	Code	Title	Description
2004-02-08	KB	Patent renewed
2006-03-12	EXP	Patent expired

Publication	Publication Date	Title
US5668298A (en)	1997-09-16	Selectable marker for development of vectors and transformation systems in plants
EP0186425B1 (fr)	1992-09-30	Marqueur de sélection pour développer des vecteurs et des systèmes de transformation de plantes
US5591605A (en)	1997-01-07	Plant structural gene expression
US5463174A (en)	1995-10-31	Transformation and foreign gene expression in Brassica species
US5750871A (en)	1998-05-12	Transformation and foreign gene expression in Brassica species
US5504200A (en)	1996-04-02	Plant gene expression
US6376234B1 (en)	2002-04-23	Method of inserting viral DNA into plant material
EP0270615B1 (fr)	1993-08-04	TRANSFORMATION DE CULTURES DE CELLULES ET EXPRESSION D'UN GENE ETRANGER DANS DES ESPECES $i(BRASSICA)
US20120192318A1 (en)	2012-07-26	Transformation system for Camelina sativa
Arokiaraj et al.	1998	CaMV 35S promoter directs β-glucuronidase expression in the laticiferous system of transgenic Hevea brasiliensis (rubber tree)
NZ207765A (en)	1987-03-06	Plant expression of transferred dna(t-dna)from plasmids associated with agrobacterium sp
JPH09238690A (ja)	1997-09-16	殺虫性タンパク質を発現する遺伝子、該タンパク質の製法および使用法
JP2001029092A (ja)	2001-02-06	植物細胞ゲノムへの発現可能な遺伝子の導入法
US20090151023A1 (en)	2009-06-11	Transformation system for Camelina sativa
US5789656A (en)	1998-08-04	Transgenic plants belonging to the species Cucumis melo
AU616444B2 (en)	1991-10-31	Insecticidal cotton plant cells
US6294712B1 (en)	2001-09-25	Nematode-resistant gene
EP0126546B2 (fr)	1994-03-30	Expression de gènes structurels de plantes
Mercuri et al.	2001	Modification of plant architecture in Limonium spp. induced by rol genes
TW319679B (fr)	1997-11-11
WO1990003725A1 (fr)	1990-04-19	Transformation de concombre par agrobacterium tumafaciens et regeneration de plantes de concombre transformees
JP2574130B2 (ja)	1997-01-22	植物細胞の遺伝的修飾方法
US5340730A (en)	1994-08-23	Process for transforming gladiolus
CA1341254C (fr)	2001-06-19	Methode genetique de modification d'une cellule vegetale, afin de permettre l'expression d'un gene de structure vegetal