IL78571A - Human tissue plasminogen activator mutants resistant to enzymatic cleavage resulting in two-chain form - Google Patents

Human tissue plasminogen activator mutants resistant to enzymatic cleavage resulting in two-chain form

Info

Publication number
IL78571A
IL78571A IL78571A IL7857186A IL78571A IL 78571 A IL78571 A IL 78571A IL 78571 A IL78571 A IL 78571A IL 7857186 A IL7857186 A IL 7857186A IL 78571 A IL78571 A IL 78571A
Authority
IL
Israel
Prior art keywords
plasminogen activator
human tissue
tissue plasminogen
vectors
enzymatic cleavage
Prior art date
Application number
IL78571A
Other languages
English (en)
Other versions
IL78571A0 (en
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of IL78571A0 publication Critical patent/IL78571A0/xx
Publication of IL78571A publication Critical patent/IL78571A/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
IL78571A 1985-04-22 1986-04-21 Human tissue plasminogen activator mutants resistant to enzymatic cleavage resulting in two-chain form IL78571A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US72546885A 1985-04-22 1985-04-22
US84669786A 1986-04-01 1986-04-01

Publications (2)

Publication Number Publication Date
IL78571A0 IL78571A0 (en) 1986-08-31
IL78571A true IL78571A (en) 1991-06-30

Family

ID=27111150

Family Applications (1)

Application Number Title Priority Date Filing Date
IL78571A IL78571A (en) 1985-04-22 1986-04-21 Human tissue plasminogen activator mutants resistant to enzymatic cleavage resulting in two-chain form

Country Status (21)

Country Link
US (1) US5147643A (ja)
EP (1) EP0199574B1 (ja)
JP (2) JPH0740940B2 (ja)
AT (1) ATE68825T1 (ja)
AU (1) AU596356B2 (ja)
CA (1) CA1341580C (ja)
DD (1) DD258026A5 (ja)
DE (2) DE3613390C2 (ja)
DK (1) DK175380B1 (ja)
ES (2) ES8802184A1 (ja)
FI (1) FI98930C (ja)
FR (1) FR2581652B1 (ja)
GB (1) GB2173804B (ja)
GR (1) GR861037B (ja)
HU (1) HU206520B (ja)
IE (1) IE58574B1 (ja)
IL (1) IL78571A (ja)
MY (1) MY102550A (ja)
NO (1) NO175216C (ja)
NZ (1) NZ215920A (ja)
PT (1) PT82429B (ja)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58149871A (ja) * 1982-02-27 1983-09-06 本田技研工業株式会社 二，三輪車の盗難防止装置
JPS6087751A (ja) * 1983-10-20 1985-05-17 House Food Ind Co Ltd レトルト豆腐の製造法
EP0201153A3 (en) * 1985-02-09 1987-10-07 Beecham Group Plc Modified enzyme and process for its preparation
WO1986005096A1 (en) * 1985-03-06 1986-09-12 Survival Technology, Inc. Protein absorption enhancing agents
US5756093A (en) * 1985-04-22 1998-05-26 Genentech, Inc. Tissue plasminogen activator variants
US5736134A (en) * 1985-04-22 1998-04-07 Genentech, In.C Tissue plasminogen activator variants
DK175380B1 (da) * 1985-04-22 2004-09-20 Genentech Inc Mutant af human vævsplasminogenaktivator, DNA-sekvens som koder herfor, replicerbar udtrykkelsesvektor til at udtrykke denne DNA-sekvens, mikroorganisme transformeret med denne vektor, cellekulturer transformeret med denne....
WO1993012225A1 (en) * 1991-12-16 1993-06-24 Genentech, Inc. Novel tissue plasminogen activator variants
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
AU605124B2 (en) * 1985-12-23 1991-01-10 Behringwerke Aktiengesellschaft Novel peptide plasminogen activators
DK43387A (da) * 1986-01-29 1987-07-30 Beecham Group Plc Fibrinolytisk enzym
US5258298A (en) * 1986-01-31 1993-11-02 Genetics Institute, Inc. Deletion and glycosylation mutant of human tissue plasminogen activator
US5071972A (en) * 1986-01-31 1991-12-10 Genetics Institute, Inc. DNA sequences encoding novel thrombolytic proteins
US5837518A (en) * 1986-01-31 1998-11-17 Genetics Institute, Inc. Thrombolytic proteins
JP2527454B2 (ja) * 1986-01-31 1996-08-21 ジェネティックス・インスチチュ−ト・インコ−ポレ−テッド 新しい血栓溶解タンパク質
IE81073B1 (en) * 1986-03-18 2000-01-12 Genentech Inc Modified human tissue-type plasminogen activator and its preparation
US5589361A (en) * 1986-03-18 1996-12-31 Genentech, Inc. Human tissue-type plasminogen activator variant
PT84589B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
PT84588B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
US5169763A (en) * 1986-04-08 1992-12-08 Transgene S.A., Institut Pasteur Viral vector coding glycoprotein of HIV-1
GB8701811D0 (en) * 1987-01-28 1987-03-04 Beecham Group Plc Compounds
MY103358A (en) * 1987-04-15 1993-06-30 Novartis Ag Process for the production of protiens.
US5200340A (en) * 1987-05-22 1993-04-06 Zymogenetics, Inc. Thrombin-activated tissue plasminogen activators
EP0292009A1 (en) * 1987-05-22 1988-11-23 Zymogenetics, Inc. Fibrinolytic proteins
ZA883958B (en) * 1987-06-04 1989-07-26 Zymogenetics Inc Mutant t-pa with ringle replacement
NO179874C (no) * 1987-06-04 1997-01-02 Zymogenetics Inc Fremgangsmåte for fremstilling av en t-PA-analog, DNA-sekvens som koder for t-PA-analogen, samt ekspresjonsvektor omfattende DNA-sekvensen og vertcelle omfattende denne
JPH02504102A (ja) * 1987-06-30 1990-11-29 ジェネンテク，インコーポレイテッド 血管障害治療のための改良法
US5302390A (en) * 1987-07-01 1994-04-12 Beecham Group Plc Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
IL87276A (en) * 1987-08-03 1995-07-31 Fujisawa Pharmaceutical Co Analog of tissue plasminogen activator comprising only the kringle 2 and protease domain dna encoding the same processes for the preparation thereof and pharmaceutical compositions containing the same
JP2708749B2 (ja) * 1987-08-10 1998-02-04 エーザイ株式会社 修飾型ｔＰＡ含有注射用組成物
US4963357A (en) * 1987-10-09 1990-10-16 Monsanto Company Tissue plasminogen activator modified by the substitution of Arg for Cys at position 73, methods and pharmaceutical compositions therefor
US5244676A (en) * 1987-10-09 1993-09-14 Monsanto Company Modified tissue plasminogen activator with modified glycosylation site
US5037752A (en) * 1987-10-09 1991-08-06 Monsanto Company Modified tissue plasminogen activator substituted at cysteine-73 and lysine-277
EP0312346B1 (en) * 1987-10-16 1994-08-17 Genencor International, Inc. E. coli sequence specific acid protease
KR970005046B1 (ko) * 1987-10-29 1997-04-11 야마노우찌 세이야꾸 가부시끼가이샤 개량형 조직 플라스미노겐 활성화 인자
US5504001A (en) * 1987-11-25 1996-04-02 Zymogenetics, Inc. Hybrid plasminogen activator
US5094953A (en) * 1988-03-21 1992-03-10 Genentech, Inc. Human tissue plasminogen activator variants
CA1320162C (en) * 1988-08-11 1993-07-13 California Biotechnology Inc. Method for stabilizing heterologous protein expression and vectors for use therein
US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5108901A (en) * 1988-09-02 1992-04-28 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5258180A (en) * 1988-09-02 1993-11-02 Genetech, Inc. Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5087572A (en) * 1989-12-01 1992-02-11 Genentech, Inc. Dna encoding human plasminogen modified at the cleavage site
US5190756A (en) * 1989-12-01 1993-03-02 Genentech, Inc. Methods and materials for expression of human plasminogen variant
DE3942142A1 (de) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh Stabilisierung von glykosyliertem t-pa
ATE145669T1 (de) * 1991-12-16 1996-12-15 Genentech Inc T-pa substitutionsvarianten mit verbesserter fibrinspezifität
DK0643772T3 (da) * 1992-06-03 1998-02-02 Genentech Inc Glycosyleringsvarianter af vævsplasminogenaktivator med forbedrede terapeutiske egenskaber
GB2298821A (en) * 1995-03-15 1996-09-18 Prestek Ltd A ribbon winding mechanism
DE69719265T2 (de) * 1996-09-06 2003-12-04 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute, Kumamoto Medizinische Zusammensetzung die Gewebeplasminogenaktivator und Nikotinamid enthält
CN1244894A (zh) * 1996-11-12 2000-02-16 斯克里普斯研究所 组织型纤溶酶原活化剂(t－PA)变体:组合物及使用方法
US6706504B1 (en) 1996-11-12 2004-03-16 The Scripps Research Institute Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
US5837688A (en) * 1996-11-27 1998-11-17 Gelfand; Mathew I. Use of thrombolytic reagents for prevention of vascular disease
WO2002020802A1 (en) * 2000-09-04 2002-03-14 Hunan Royal Biotech A cell line expressing mutant human tissue-type plasminogen activator, constructing strategy and method of expressed protein preparation
US7597895B2 (en) * 2001-02-20 2009-10-06 The Governing Council Of The University Of Toronto Signal peptides, nucleic acid molecules and methods for treatment of caries
US7556807B2 (en) 2001-02-20 2009-07-07 Kane Biotech, Inc. Signal peptides, nucleic acid molecules and methods for treatment of caries

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GT198302091A (es) * 1982-05-05 1984-10-25 Activador de plasminogeno de tejido humano a),b),c).
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
IL90047A (en) * 1982-10-19 1992-12-01 Cetus Oncology Corp Cysteine-depleted biologically active muteins other than interferon - '
US4753879A (en) * 1984-08-27 1988-06-28 Biogen N.V. Modified tissue plasminogen activators
EP0201153A3 (en) * 1985-02-09 1987-10-07 Beecham Group Plc Modified enzyme and process for its preparation
DK175380B1 (da) * 1985-04-22 2004-09-20 Genentech Inc Mutant af human vævsplasminogenaktivator, DNA-sekvens som koder herfor, replicerbar udtrykkelsesvektor til at udtrykke denne DNA-sekvens, mikroorganisme transformeret med denne vektor, cellekulturer transformeret med denne....
DK43387A (da) * 1986-01-29 1987-07-30 Beecham Group Plc Fibrinolytisk enzym
JP2527454B2 (ja) * 1986-01-31 1996-08-21 ジェネティックス・インスチチュ−ト・インコ−ポレ−テッド 新しい血栓溶解タンパク質

Also Published As

Publication number Publication date
US5147643A (en) 1992-09-15
ES9000011A1 (es) 1990-03-01
GB2173804A (en) 1986-10-22
HUT40703A (en) 1987-01-28
AU5641686A (en) 1986-10-30
IL78571A0 (en) 1986-08-31
GR861037B (en) 1986-07-16
PT82429B (pt) 1988-03-03
FR2581652A1 (fr) 1986-11-14
IE58574B1 (en) 1993-10-06
FI861673A0 (fi) 1986-04-21
ATE68825T1 (de) 1991-11-15
DK181286D0 (da) 1986-04-21
NO861559L (no) 1986-10-23
AU596356B2 (en) 1990-05-03
DE3613390A1 (de) 1986-10-30
ES8802184A1 (es) 1988-04-16
FI861673L (fi) 1986-10-23
EP0199574B1 (en) 1991-10-23
FR2581652B1 (fr) 1989-12-22
DE3613390C2 (de) 2001-10-11
JP2529816B2 (ja) 1996-09-04
DK175380B1 (da) 2004-09-20
FI98930B (fi) 1997-05-30
EP0199574A3 (en) 1987-08-05
DE3682104D1 (de) 1991-11-28
DK181286A (da) 1986-10-23
NO175216B (ja) 1994-06-06
HU206520B (en) 1992-11-30
JPS6224A (ja) 1987-01-06
DD258026A5 (de) 1988-07-06
PT82429A (en) 1986-05-01
GB8609683D0 (en) 1986-05-29
GB2173804B (en) 1989-07-05
NZ215920A (en) 1989-07-27
JPH0740940B2 (ja) 1995-05-10
CA1341580C (en) 2008-09-30
JPH06277071A (ja) 1994-10-04
IE861055L (en) 1986-10-22
ES554251A0 (es) 1988-04-16
FI98930C (fi) 1997-09-10
MY102550A (en) 1992-07-31
NO175216C (no) 1994-09-14
EP0199574A2 (en) 1986-10-29
ES557800A0 (es) 1990-03-01

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