IL88559A - Vectors and compounds for direct expression of human activated C protein - Google Patents

Vectors and compounds for direct expression of human activated C protein

Info

Publication number: IL88559A
Authority: IL; Israel
Prior art keywords: leu; ser; gly; glu; arg
Prior art date: 1987-12-04

Application number

IL8855988A

Other languages

English (en)

Hebrew (he)

Other versions

IL88559A0 (en

Original Assignee

Lilly Co Eli

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-12-04

Filing date

1988-12-01

Publication date

1994-02-27

1988-12-01 Application filed by Lilly Co Eli filed Critical Lilly Co Eli

1989-07-31 Publication of IL88559A0 publication Critical patent/IL88559A0/xx

1994-02-27 Publication of IL88559A publication Critical patent/IL88559A/en

Links

229940100689 human protein c Drugs 0.000 title claims description 78
101500025568 Homo sapiens Saposin-D Proteins 0.000 title claims description 77
239000013598 vector Substances 0.000 title claims description 56
150000001875 compounds Chemical class 0.000 title claims description 34
239000013612 plasmid Substances 0.000 claims description 256
108020004414 DNA Proteins 0.000 claims description 219
210000004027 cell Anatomy 0.000 claims description 166
101800004937 Protein C Proteins 0.000 claims description 116
229960000856 protein c Drugs 0.000 claims description 105
101800001700 Saposin-D Proteins 0.000 claims description 103
238000000034 method Methods 0.000 claims description 77
108091026890 Coding region Proteins 0.000 claims description 53
108090000623 proteins and genes Proteins 0.000 claims description 53
102000004169 proteins and genes Human genes 0.000 claims description 36
235000018102 proteins Nutrition 0.000 claims description 35
238000003776 cleavage reaction Methods 0.000 claims description 29
230000007017 scission Effects 0.000 claims description 28
102000010911 Enzyme Precursors Human genes 0.000 claims description 25
108010062466 Enzyme Precursors Proteins 0.000 claims description 25
239000013604 expression vector Substances 0.000 claims description 21
108090000765 processed proteins & peptides Proteins 0.000 claims description 21
108091028043 Nucleic acid sequence Proteins 0.000 claims description 19
125000000539 amino acid group Chemical group 0.000 claims description 17
102000004196 processed proteins & peptides Human genes 0.000 claims description 15
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims description 14
229920001184 polypeptide Polymers 0.000 claims description 14
239000004475 Arginine Substances 0.000 claims description 13
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DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 13
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 13
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CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 12
101800001401 Activation peptide Proteins 0.000 claims description 11
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DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims description 10
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims description 10
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150000001413 amino acids Chemical class 0.000 claims description 7
235000013922 glutamic acid Nutrition 0.000 claims description 7
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WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 6
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QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 6
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239000004473 Threonine Substances 0.000 claims description 6
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6464—Protein C (3.4.21.69)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site

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Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Medicinal Chemistry (AREA)
Toxicology (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Enzymes And Modification Thereof (AREA)

IL8855988A 1987-12-04 1988-12-01 Vectors and compounds for direct expression of human activated C protein IL88559A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US07/129,027 US4992373A (en)	1987-12-04	1987-12-04	Vectors and compounds for direct expression of activated human protein C

Publications (2)

Publication Number	Publication Date
IL88559A0 IL88559A0 (en)	1989-07-31
IL88559A true IL88559A (en)	1994-02-27

Family

ID=22438133

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL8855988A IL88559A (en)	1987-12-04	1988-12-01	Vectors and compounds for direct expression of human activated C protein

Country Status (20)

Country	Link
US (1)	US4992373A (ja)
EP (1)	EP0319312B1 (ja)
JP (1)	JP2749083B2 (ja)
KR (1)	KR970009081B1 (ja)
CN (1)	CN1035527A (ja)
AR (1)	AR245217A1 (ja)
AT (1)	ATE96840T1 (ja)
AU (1)	AU610301B2 (ja)
CA (1)	CA1339938C (ja)
DE (1)	DE3885419T2 (ja)
DK (1)	DK672788A (ja)
ES (1)	ES2059538T3 (ja)
HU (1)	HU209587B (ja)
IE (1)	IE61627B1 (ja)
IL (1)	IL88559A (ja)
MX (1)	MX166847B (ja)
NZ (1)	NZ227177A (ja)
PT (1)	PT89135B (ja)
RU (1)	RU1830081C (ja)
ZA (1)	ZA889033B (ja)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS6437298A (en) *	1987-08-04	1989-02-07	Hiroshi Okamoto	Production of c-terminal amidated peptide
JP3045307B2 (ja) *	1989-08-11	2000-05-29	ザイモジェネティクス，インコーポレイティド	活性化プロテインｃを生成するための細胞培養法
ATE163048T1 (de) *	1989-12-29	1998-02-15	Zymogenetics Inc	Hybrides protein c
IL97311A0 (en) *	1990-02-23	1992-05-25	Lilly Co Eli	Vectors and compounds for expression of glycosylation mutants of human protein c
US5270178A (en) *	1990-02-23	1993-12-14	Eli Lilly And Company	Vectors and compounds for expression of zymogen forms of human protein C
IL97312A (en) *	1990-02-23	1999-01-26	Lilly Co Eli	A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
US5326700A (en) *	1990-11-06	1994-07-05	Eli Lilly And Company	DNA sequences encoding t-PA derivatives with cleavable sites
JP4236698B2 (ja) *	1990-11-26	2009-03-11	ジェネティックスインスティチュート，リミテッドライアビリティカンパニー	宿主細胞でのｐａｃｅの発現およびその使用法
AU1228592A (en) *	1991-01-11	1992-08-17	American Red Cross	Expression of active human protein c in mammary tissue of transgenic animals
US5831141A (en) *	1991-01-11	1998-11-03	United States Of America As Represented By The Department Of Health And Human Services	Expression of a heterologous polypeptide in mammary tissue of transgenic non-human mammals using a long whey acidic protein promoter
US6262336B1 (en)	1991-01-11	2001-07-17	American Red Cross	Expression of a heterologous protein C in mammary tissue of transgenic animals using a long whey acidic protein promoter
US5965789A (en) *	1991-01-11	1999-10-12	American Red Cross	Engineering protein posttranslational modification by PACE/furin in transgenic non-human mammals
MY110664A (en) *	1992-05-21	1999-01-30	Lilly Co Eli	Protein c derivatives
US5464934A (en) *	1993-05-14	1995-11-07	Mallinckrodt Medical, Inc.	Metal chelates as spacer compounds in biologically active peptides
US5618714A (en) *	1993-12-15	1997-04-08	Eli Lilly And Company	Methods for producing protein C
US5698444A (en) *	1993-12-23	1997-12-16	Eli Lilly And Company	Serotonin receptor protein and related nucleic acid compounds
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1987
- 1987-12-04 US US07/129,027 patent/US4992373A/en not_active Expired - Lifetime
1988
- 1988-12-01 HU HU886142A patent/HU209587B/hu not_active IP Right Cessation
- 1988-12-01 AR AR88312611A patent/AR245217A1/es active
- 1988-12-01 ZA ZA889033A patent/ZA889033B/xx unknown
- 1988-12-01 IL IL8855988A patent/IL88559A/en not_active IP Right Cessation
- 1988-12-02 ES ES88311421T patent/ES2059538T3/es not_active Expired - Lifetime
- 1988-12-02 PT PT89135A patent/PT89135B/pt active IP Right Grant
- 1988-12-02 DE DE88311421T patent/DE3885419T2/de not_active Expired - Fee Related
- 1988-12-02 IE IE361288A patent/IE61627B1/en not_active IP Right Cessation
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- 1988-12-02 AT AT88311421T patent/ATE96840T1/de not_active IP Right Cessation
- 1988-12-02 AU AU26509/88A patent/AU610301B2/en not_active Ceased
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- 1988-12-03 CN CN88108295A patent/CN1035527A/zh active Pending
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Also Published As

Publication number	Publication date
DE3885419D1 (de)	1993-12-09
ATE96840T1 (de)	1993-11-15
IL88559A0 (en)	1989-07-31
ES2059538T3 (es)	1994-11-16
PT89135A (pt)	1989-12-29
HUT50497A (en)	1990-02-28
KR970009081B1 (en)	1997-06-05
AR245217A1 (es)	1993-12-30
DK672788D0 (da)	1988-12-02
EP0319312B1 (en)	1993-11-03
HU209587B (en)	1994-08-29
ZA889033B (en)	1990-08-29
US4992373A (en)	1991-02-12
JP2749083B2 (ja)	1998-05-13
PT89135B (pt)	1994-04-29
AU2650988A (en)	1989-06-08
DK672788A (da)	1989-07-28
EP0319312A2 (en)	1989-06-07
IE883612L (en)	1989-06-04
CN1035527A (zh)	1989-09-13
RU1830081C (ru)	1993-07-23
NZ227177A (en)	1990-07-26
IE61627B1 (en)	1994-11-16
KR890010199A (ko)	1989-08-07
EP0319312A3 (en)	1990-09-19
DE3885419T2 (de)	1994-03-31
MX166847B (es)	1993-02-09
JPH022372A (ja)	1990-01-08
CA1339938C (en)	1998-07-07
AU610301B2 (en)	1991-05-16

Legal Events

Date	Code	Title	Description
2003-09-17	RH1	Patent not in force

Publication	Publication Date	Title
US4992373A (en)	1991-02-12	Vectors and compounds for direct expression of activated human protein C
US5225537A (en)	1993-07-06	Methods for producing hybrid phospholipid-binding proteins
US5516650A (en)	1996-05-14	Production of activated protein C
CA1340263C (en)	1998-12-15	Expression of protein c analogues
US4784950A (en)	1988-11-15	Expression of factor VII activity in mammalian cells
EP0215548B1 (en)	1993-08-18	Expression of protein c
US5196322A (en)	1993-03-23	Vectors and compounds for expression of zymogen forms of human protein C
US5358932A (en)	1994-10-25	Hybrid protein C
CA2036915A1 (en)	1991-08-24	Vectors and compounds for expression of zymogen forms of human protein c
CA2036894C (en)	2002-01-15	Vectors and compounds for expression of glycosylation mutants of human protein c
US5766921A (en)	1998-06-16	Hybrid protein C
US5326700A (en)	1994-07-05	DNA sequences encoding t-PA derivatives with cleavable sites
EP0323149B1 (en)	1994-12-28	Vectors and compounds for expression of zymogen forms of human protein C
CA2058079A1 (en)	1992-06-25	Method of treating thromboembolic disorders
WO1991012320A1 (en)	1991-08-22	Activated protein c with truncated light chain
EP0485504B1 (en)	1993-12-01	Cell culture methods for producing activated protein c
JP3153236B2 (ja)	2001-04-03	端が切り取られた軽鎖を有する組換えプロテインｃ