IL90832A - A genetic sequence induces a change after the translation of the amino acids and polypeptide encoded by it - Google Patents

A genetic sequence induces a change after the translation of the amino acids and polypeptide encoded by it

Info

Publication number: IL90832A
Authority: IL; Israel
Prior art keywords: polypeptide; sequence; precursor; gat; aaa
Prior art date: 1988-07-05

Application number

IL9083289A

Other languages

English (en)

Hebrew (he)

Other versions

IL90832A0 (en

Original Assignee

Univ Maryland

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-07-05

Filing date

1989-06-30

Publication date

1995-06-29

1989-06-30 Application filed by Univ Maryland filed Critical Univ Maryland

1990-01-18 Publication of IL90832A0 publication Critical patent/IL90832A0/xx

1995-06-29 Publication of IL90832A publication Critical patent/IL90832A/en

Links

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102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 46
229920001184 polypeptide Polymers 0.000 title claims abstract description 41
150000001413 amino acids Chemical class 0.000 title claims abstract description 37
230000004481 post-translational protein modification Effects 0.000 title claims abstract description 28
108091028043 Nucleic acid sequence Proteins 0.000 title claims description 21
108090000623 proteins and genes Proteins 0.000 claims abstract description 71
241000894006 Bacteria Species 0.000 claims abstract description 16
238000000034 method Methods 0.000 claims abstract description 16
239000002243 precursor Substances 0.000 claims description 41
102000004169 proteins and genes Human genes 0.000 claims description 39
108010082567 subtilin Proteins 0.000 claims description 33
108010053775 Nisin Proteins 0.000 claims description 31
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CRJZZXMAADSBBQ-SRVKXCTJSA-N Ser-Lys-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CO CRJZZXMAADSBBQ-SRVKXCTJSA-N 0.000 claims 1
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CXTXHTVXPMOOSW-JUEJINBGSA-N epidermin Chemical compound C([C@H]1C(=O)N[C@H](C(=O)N[C@@H](CSC[C@H](C(N[C@@H](CCCCN)C(=O)N1)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)[C@@H](C)CC)C(=O)N[C@H]1C(N2CCC[C@H]2C(=O)NCC(=O)N[C@@H](CS[C@H]1C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N\C(=C/C)C(=O)NCC(=O)N[C@H]1C(N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H]2C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@H](C(N\C=C/SC2)=O)CSC1)=O)=O)[C@@H](C)CC)C1=CC=CC=C1 CXTXHTVXPMOOSW-JUEJINBGSA-N 0.000 description 1
108010064962 epidermin Proteins 0.000 description 1
125000003700 epoxy group Chemical group 0.000 description 1
239000000284 extract Substances 0.000 description 1
238000005194 fractionation Methods 0.000 description 1
239000008103 glucose Substances 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
238000011534 incubation Methods 0.000 description 1
239000003112 inhibitor Substances 0.000 description 1
239000002054 inoculum Substances 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
238000002372 labelling Methods 0.000 description 1
239000008101 lactose Substances 0.000 description 1
239000011565 manganese chloride Substances 0.000 description 1
235000002867 manganese chloride Nutrition 0.000 description 1
108020004999 messenger RNA Proteins 0.000 description 1
238000002156 mixing Methods 0.000 description 1
108010009719 mutanolysin Proteins 0.000 description 1
230000035772 mutation Effects 0.000 description 1
239000013600 plasmid vector Substances 0.000 description 1
229920000647 polyepoxide Polymers 0.000 description 1
239000003910 polypeptide antibiotic agent Substances 0.000 description 1
239000000843 powder Substances 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
230000009465 prokaryotic expression Effects 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
239000011833 salt mixture Substances 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000012216 screening Methods 0.000 description 1
235000004400 serine Nutrition 0.000 description 1
150000003355 serines Chemical class 0.000 description 1
235000020183 skimmed milk Nutrition 0.000 description 1
230000008685 targeting Effects 0.000 description 1
150000003568 thioethers Chemical class 0.000 description 1
235000008521 threonine Nutrition 0.000 description 1
150000003588 threonines Chemical class 0.000 description 1
235000015193 tomato juice Nutrition 0.000 description 1
239000003053 toxin Substances 0.000 description 1
231100000765 toxin Toxicity 0.000 description 1
108700012359 toxins Proteins 0.000 description 1
230000014621 translational initiation Effects 0.000 description 1
239000013598 vector Substances 0.000 description 1
239000011592 zinc chloride Substances 0.000 description 1
235000005074 zinc chloride Nutrition 0.000 description 1
JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

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Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Biophysics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Medicinal Chemistry (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Crystallography & Structural Chemistry (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

IL9083289A 1988-07-05 1989-06-30 A genetic sequence induces a change after the translation of the amino acids and polypeptide encoded by it IL90832A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US07/214,959 US5218101A (en)	1988-07-05	1988-07-05	Leader sequence inducing a post-translational modification of polypeptides in bacteria, and gene therefor

Publications (2)

Publication Number	Publication Date
IL90832A0 IL90832A0 (en)	1990-01-18
IL90832A true IL90832A (en)	1995-06-29

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ID=22801074

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL9083289A IL90832A (en)	1988-07-05	1989-06-30	A genetic sequence induces a change after the translation of the amino acids and polypeptide encoded by it

Country Status (14)

Country	Link
US (1)	US5218101A (da)
EP (1)	EP0423224B1 (da)
JP (2)	JP3326172B2 (da)
KR (1)	KR0137961B1 (da)
AT (1)	ATE175417T1 (da)
AU (1)	AU640110B2 (da)
CA (1)	CA1339414C (da)
DE (2)	DE68928899D1 (da)
DK (1)	DK191D0 (da)
IL (1)	IL90832A (da)
NO (1)	NO309200B1 (da)
NZ (1)	NZ229538A (da)
WO (1)	WO1990000558A1 (da)
ZA (1)	ZA894721B (da)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5516682A (en) *	1988-07-05	1996-05-14	University Of Maryland	Subtilin variant of enhanced stability and activity
US5348881A (en) *	1990-03-13	1994-09-20	Quest International Flavors & Good Ingredients Company, Division Of Indopco, Inc.	Multiple bacteriocin producing lactococcus and compositions
NL9000753A (nl) *	1990-03-30	1991-10-16	Nl Zuivelonderzoek Inst	Dna-fragment, ten minste coderend voor een deel van het extracellulaire eiwit met een schijnbaar molecuulgewicht van 60 kda van lactococcus lactis stammen zoals de lactococcus lactis ssp. lactis stam mg1363, combinaties hiervan met voor homologe of heterologe eiwitten coderende dna-sequenties alsmede de hiermee verkregen homologe en heterologe eiwitten.
WO1992018633A1 (en) *	1991-04-11	1992-10-29	Stichting Nederlands Instituut Voor Zuivelonderzoek (Nizo)	Lantibiotics similar to nisin a, lactic acid bacteria which produce such lantibiotics, method for constructing such lactic acid bacteria and method for preserving foodstuffs with the aid of these lantibiotics and these lactic acid bacteria producing lantibiotics
US5861376A (en) *	1991-07-01	1999-01-19	Quest International Flavors & Food Ingredients Company, Division Of Indopco, Inc.	Synthetically derived peptide
GB9207267D0 (en) *	1992-04-02	1992-05-13	Agricultural & Food Res	Nisins
ES2207636T3 (es) *	1994-09-12	2004-06-01	Aventis Pharma Deutschland Gmbh	Mersacidina recombinante y un metodo para su produccion.
US5919695A (en) *	1997-04-22	1999-07-06	Quest International, B.V.	Method and culture for inhibiting undesired microorganisms with culture of Bacillus subtilis
WO1999003352A1 (en) *	1997-07-18	1999-01-28	University Of Maryland	Sublancin lantibiotic produced by bacillus subtilis 168
US6953683B2 (en) *	1997-07-18	2005-10-11	University Of Maryland	Compositions and methods of inhibiting bacterial spore germination
WO2000039152A1 (en) *	1998-12-24	2000-07-06	University Of Maryland	Stable subtilin and methods of manufacture
US7033766B2 (en) *	2000-06-29	2006-04-25	University Of Maryland Office Of Technology Commercialization	Construction and screening of lantibody display libraries
US6759205B2 (en)	2000-06-29	2004-07-06	University Of Maryland	Construction of a strain of Bacillus subtilis 168 that displays the sublancin lantibiotic on the surface of the cell
US6846804B2 (en) *	2000-06-29	2005-01-25	University Of Maryland, College Park Office Of Technology Commercialization	Construction of a structural variant of sublancin to facilitate its isolation and use in bioremediation of environmental contamination by gram-positive spore formers such as bacillus anthrasis
WO2003063887A1 (en) *	2002-01-14	2003-08-07	University Of Maryland, College Park	Compositions and method of inhibiting bacterial spore germination
US6861236B2 (en) *	2002-05-24	2005-03-01	Applied Nanosystems B.V.	Export and modification of (poly)peptides in the lantibiotic way
DK1819816T3 (da)	2004-12-07	2009-01-26	Applied Nanosystems Bv	Fremgangsmåder til fremstilling og udskillelse af modificerede peptider
KR20080106545A (ko) *	2006-02-13	2008-12-08	베링거잉겔하임베트메디카게엠베하	란티바이오틱의 제조 방법
US7910695B2 (en) *	2006-06-29	2011-03-22	The Invention Science Fund I, Llc	Methods for arbitrary peptide synthesis
US7879975B2 (en) *	2006-06-29	2011-02-01	The Invention Science Fund I, Llc	Methods for arbitrary peptide synthesis
US7923533B2 (en) *	2006-06-29	2011-04-12	The Invention Science Fund I, Llc	Methods for arbitrary peptide synthesis
US7993873B2 (en) *	2006-06-29	2011-08-09	The Invention Science Fund I, Llc	Apparatus for arbitrary peptide synthesis

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4716115A (en) *	1983-09-06	1987-12-29	Microlife Technics, Inc.	Derived nisin producing microorganisms, method of production and use and products obtained thereby

1988
- 1988-07-05 US US07/214,959 patent/US5218101A/en not_active Expired - Fee Related
1989
- 1989-06-14 NZ NZ229538A patent/NZ229538A/en unknown
- 1989-06-21 ZA ZA894721A patent/ZA894721B/xx unknown
- 1989-06-30 JP JP50801189A patent/JP3326172B2/ja not_active Expired - Fee Related
- 1989-06-30 KR KR1019900700460A patent/KR0137961B1/ko not_active Expired - Fee Related
- 1989-06-30 AU AU39684/89A patent/AU640110B2/en not_active Ceased
- 1989-06-30 DE DE68928899A patent/DE68928899D1/de not_active Expired - Fee Related
- 1989-06-30 DE DE68928899T patent/DE68928899T4/de not_active Expired - Lifetime
- 1989-06-30 AT AT89908558T patent/ATE175417T1/de not_active IP Right Cessation
- 1989-06-30 IL IL9083289A patent/IL90832A/en not_active IP Right Cessation
- 1989-06-30 EP EP89908558A patent/EP0423224B1/en not_active Expired - Lifetime
- 1989-06-30 WO PCT/US1989/002820 patent/WO1990000558A1/en not_active Ceased
- 1989-07-04 CA CA000604753A patent/CA1339414C/en not_active Expired - Fee Related
1990
- 1990-12-28 NO NO905638A patent/NO309200B1/no not_active IP Right Cessation
1991
- 1991-01-02 DK DK000191A patent/DK191D0/da not_active Application Discontinuation
2001
- 2001-11-08 JP JP2001343857A patent/JP3418388B2/ja not_active Expired - Fee Related

Also Published As

Publication number	Publication date
AU640110B2 (en)	1993-08-19
CA1339414C (en)	1997-09-02
NO309200B1 (no)	2000-12-27
KR900701819A (ko)	1990-12-04
DE68928899D1 (de)	1999-02-18
DK191A (da)	1991-01-02
JP3326172B2 (ja)	2002-09-17
IL90832A0 (en)	1990-01-18
DE68928899T2 (de)	1999-08-12
EP0423224A4 (en)	1991-11-06
ZA894721B (en)	1990-03-28
NO905638D0 (no)	1990-12-28
JPH03505670A (ja)	1991-12-12
JP3418388B2 (ja)	2003-06-23
AU3968489A (en)	1990-02-05
EP0423224A1 (en)	1991-04-24
NO905638L (no)	1990-12-28
WO1990000558A1 (en)	1990-01-25
KR0137961B1 (ko)	1998-04-30
JP2002191383A (ja)	2002-07-09
DE68928899T4 (de)	1999-11-25
DK191D0 (da)	1991-01-02
ATE175417T1 (de)	1999-01-15
EP0423224B1 (en)	1999-01-07
NZ229538A (en)	1992-01-29
US5218101A (en)	1993-06-08

Legal Events

Date	Code	Title
1996-03-31	KB	Patent renewed
1999-12-22	KB	Patent renewed
2004-02-19	RH1	Patent not in force

Publication	Publication Date	Title
US5218101A (en)	1993-06-08	Leader sequence inducing a post-translational modification of polypeptides in bacteria, and gene therefor
US5885811A (en)	1999-03-23	Leader sequence inducing a post-translational modification of polypeptides in bacteria gene therefor and subtilin variant of enhanced stability and activity
Buchman et al.	1988	Structure, expression, and evolution of a gene encoding the precursor of nisin, a small protein antibiotic.
Matsushita et al.	1998	A Study of the Collagen-binding Domain of a 116-kDaClostridium histolyticum Collagenase
US5821088A (en)	1998-10-13	Use of gram-positive bacteria to express recombinant proteins
Kupke et al.	1992	Purification and characterization of EpiD, a flavoprotein involved in the biosynthesis of the lantibiotic epidermin
KR100530598B1 (ko)	2005-11-23	초내열성 프로테아제 발현계
Nagasawa et al.	1989	Determination of the cleavage site involved in C-terminal processing of penicillin-binding protein 3 of Escherichia coli
US5232841A (en)	1993-08-03	Expression vectors containing a bacillus brevis signal sequence
CA2185343A1 (en)	1995-09-28	Enhanced secretion of polypeptides
Engel et al.	1992	Murein-metabolizing enzymes from Escherichia coli: existence of a second lytic transglycosylase
HU214368B (hu)	1998-03-30	Eljárás új hirudinszármazék előállítására
Lilley et al.	1992	Amino acid and DNA sequences of an extracellular basic protease of Dichelobacter nodosus show that it is a member of the subtilisin family of proteases
CA2081704C (en)	2007-05-01	Biosynthetic process for the preparation of lantibiotics
AU655312B2 (en)	1994-12-15	Isolation and characterization of a novel protease from streptomyces lividans
WO1991000913A1 (en)	1991-01-24	Inducible expression vectors
US5837485A (en)	1998-11-17	DNA encoding and biosynthetic process for the preparation of chemical compounds, lantibiotics
EP0342658B1 (en)	1994-05-11	Biosynthetic process for the preparation of chemical compounds
JPH0851982A (ja)	1996-02-27	ヒト血清アルブミンをコードする改変された遺伝子
US6077689A (en)	2000-06-20	Enhanced solubility of recombinant proteins
JPH0731480A (ja)	1995-02-03	Ｌ−グルタミルｔＲＮＡレダクターゼをコードするＤＮＡ断片
WO2004033682A1 (ja)	2004-04-22	超耐熱性エンドグルカナーゼの製造法
JPH084511B2 (ja)	1996-01-24	新規蛋白質遺伝子