IL97677A - Method for recovering fetal cells from a blood sample containing a mixed population of maternal and fetal cells - Google Patents

Method for recovering fetal cells from a blood sample containing a mixed population of maternal and fetal cells

Info

Publication number: IL97677A
Authority: IL; Israel
Prior art keywords: cells; antibody; maternal; fetal; labeled
Prior art date: 1990-03-27

Application number

IL9767791A

Other languages

English (en)

Other versions

IL97677A0 (en

Original Assignee

Genetype Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-03-27

Filing date

1991-03-26

Publication date

1996-07-23

1991-03-26 Application filed by Genetype Ag filed Critical Genetype Ag

1992-06-21 Publication of IL97677A0 publication Critical patent/IL97677A0/xx

1996-07-23 Publication of IL97677A publication Critical patent/IL97677A/en

Links

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Classifications

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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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- G—PHYSICS
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- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
- G—PHYSICS
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- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/80—Fluorescent dyes, e.g. rhodamine
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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IL9767791A 1990-03-27 1991-03-26 Method for recovering fetal cells from a blood sample containing a mixed population of maternal and fetal cells IL97677A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US07/499,932 US5153117A (en)	1990-03-27	1990-03-27	Fetal cell recovery method

Publications (2)

Publication Number	Publication Date
IL97677A0 IL97677A0 (en)	1992-06-21
IL97677A true IL97677A (en)	1996-07-23

Family

ID=23987348

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL9767791A IL97677A (en)	1990-03-27	1991-03-26	Method for recovering fetal cells from a blood sample containing a mixed population of maternal and fetal cells

Country Status (16)

Country	Link
US (2)	US5153117A (fr)
EP (1)	EP0521909B1 (fr)
JP (1)	JP2965699B2 (fr)
AT (1)	ATE194166T1 (fr)
AU (1)	AU649027B2 (fr)
CA (1)	CA2059554C (fr)
DE (1)	DE69132269T2 (fr)
DK (1)	DK0521909T3 (fr)
ES (1)	ES2149760T3 (fr)
GR (1)	GR3034487T3 (fr)
IE (1)	IE910996A1 (fr)
IL (1)	IL97677A (fr)
NZ (1)	NZ237589A (fr)
SG (1)	SG79188A1 (fr)
WO (1)	WO1991014768A1 (fr)
ZA (1)	ZA912317B (fr)

Families Citing this family (118)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH05501612A (ja) *	1989-11-13	1993-03-25	チルドレンズ・メデイカル・センター・コーポレーシヨン	胎児ｄｎａ単離および検出のための非侵入性方法
US5153117A (en) *	1990-03-27	1992-10-06	Genetype A.G.	Fetal cell recovery method
US5627029A (en) *	1991-05-03	1997-05-06	Genzyme Corporation	Method for obtaining informative cells
AU680412B2 (en) *	1992-04-09	1997-07-31	Ig Laboratories, Inc.	Probes for detecting common liveborn chromosomal aneuploidies
US5629147A (en) *	1992-07-17	1997-05-13	Aprogenex, Inc.	Enriching and identifying fetal cells in maternal blood for in situ hybridization
BR9306867A (pt) *	1992-07-17	1998-12-08	Aprogenex Inc	Enriquecimento e identificação de células fetais do sangue materno para a hibridização in situ
US5275933A (en) *	1992-09-25	1994-01-04	The Board Of Trustees Of The Leland Stanford Junior University	Triple gradient process for recovering nucleated fetal cells from maternal blood
EP0690927A4 (fr) *	1993-01-06	2002-02-13	Boehringer Mannheim Corp	Detection par fluorescence de cellules non viables
US5457024A (en) *	1993-01-22	1995-10-10	Aprogenex, Inc.	Isolation of fetal erythrocytes
AU7921194A (en) *	1993-09-27	1995-04-18	Oncor, Inc.	Methods for detecting and analyzing individual rare cells in a population
US6136540A (en) *	1994-10-03	2000-10-24	Ikonisys Inc.	Automated fluorescence in situ hybridization detection of genetic abnormalities
US5352613A (en) *	1993-10-07	1994-10-04	Tafas Triantafillos P	Cytological screening method
GB9422504D0 (en) *	1994-11-08	1995-01-04	Robertson Patricia M B	Blood testing
US5750339A (en) *	1994-11-30	1998-05-12	Thomas Jefferson University	Methods for identifying fetal cells
US5639669A (en) *	1995-06-07	1997-06-17	Ledley; Robert	Separation of fetal cells from maternal blood
CN1153064C (zh) *	1996-04-05	2004-06-09	约翰斯·霍普金斯大学	富集稀少细胞的方法
US6060240A (en) *	1996-12-13	2000-05-09	Arcaris, Inc.	Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
EP2264427B1 (fr)	1997-01-31	2017-05-03	Xy, Llc	Dispositif optique avec réflecteur focalisant pour faire converger la radiation sur un débit de particule, et procédé d'analyse associé
JP2001514751A (ja) *	1997-03-08	2001-09-11	ザ・ユニバーシティー・オブ・ダンディー	胎児診断方法
US6890755B1 (en) *	1997-12-08	2005-05-10	The Trustees Of Columbia University In The City Of New York	Recovery of fetal cells from maternal blood using chaotic advection
US6149867A (en) *	1997-12-31	2000-11-21	Xy, Inc.	Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6071689A (en)	1997-12-31	2000-06-06	Xy, Inc.	System for improving yield of sexed embryos in mammals
US6210889B1 (en) *	1998-01-28	2001-04-03	The Universite Laval	Method for enrichment of fetal cells from maternal blood and use of same in determination of fetal sex and detection of chromosomal abnormalities
US6733967B1 (en)	1998-04-21	2004-05-11	Interleukin Genetics, Inc.	Fetal testing for prediction of low birth weight
PT1917974E (pt)	1998-07-30	2011-02-22	Xy Llc	Sistema de inseminação artificial não cirúrgica em equinos
AU2686100A (en) *	1999-02-18	2000-09-04	Bio-View Ltd.	System and method for identifying and analyzing rare cell types in a mixed population of cells
WO2000071987A2 (fr) *	1999-05-25	2000-11-30	John Philip	Isolation et culture de cellules embryonnaires
EP1283904A2 (fr) *	1999-11-03	2003-02-19	Oncotech, Inc.	Methodes de prognostic et de diagnostic du cancer
US7208265B1 (en)	1999-11-24	2007-04-24	Xy, Inc.	Method of cryopreserving selected sperm cells
AU2001277635A1 (en) *	2000-03-14	2001-10-08	Exiqon A/S	New methods for separation and isolation of subpopulations of cells in biological samples
IL152714A (en)	2000-05-09	2014-03-31	Xy Llc	High-purity spermatozoa populations carrying chromosome-x and chromosome-y
WO2002043486A1 (fr)	2000-11-29	2002-06-06	Xy, Inc.	Systeme permettant de realiser une fecondation in vitro avec des spermatozoides separes en population porteuse de chromosome x et en population porteuse de chromosome y
US7713687B2 (en)	2000-11-29	2010-05-11	Xy, Inc.	System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
WO2002074055A2 (fr) *	2001-03-19	2002-09-26	Ikonisys, Inc.	Systeme et procede pour accentuer le contraste d'une image produite par un microscope a epifluorescence
US20040214314A1 (en) *	2001-11-02	2004-10-28	Friedrich Srienc	High throughput bioreactor
WO2003040064A2 (fr) *	2001-11-07	2003-05-15	Bioview Ltd.	Kits et methodes de preparation d'echantillons cellulaires optimises pour une coloration duale
IL163600A0 (en) *	2002-03-01	2005-12-18	Ravgen Inc	Methods for detection of genetic disorders
US6977162B2 (en) *	2002-03-01	2005-12-20	Ravgen, Inc.	Rapid analysis of variations in a genome
US20070178478A1 (en) *	2002-05-08	2007-08-02	Dhallan Ravinder S	Methods for detection of genetic disorders
US7442506B2 (en)	2002-05-08	2008-10-28	Ravgen, Inc.	Methods for detection of genetic disorders
US7727720B2 (en)	2002-05-08	2010-06-01	Ravgen, Inc.	Methods for detection of genetic disorders
EP1532453B1 (fr) *	2002-05-31	2013-08-21	Genetic Technologies Limited	Anticorps maternels servant de marqueurs de cellules foetales permettant d'identifier et d'enrichir les cellules foetales du sang maternel
EP1533616A4 (fr) *	2002-06-07	2007-01-17	Towa Kagaku Co Ltd	Trousse de detection, plaque d'essai biologique utilisable avec cette trousse, procede de detection, procede d'evaluation, anticorps polyclonal de la vitellogenine de la grenouille et procede de production associe
WO2003106623A2 (fr) *	2002-06-13	2003-12-24	New York University	Teste precoce prenatal non effractif pour detecter des aneuploidies et des etats pathologiques hereditaires
US8486618B2 (en)	2002-08-01	2013-07-16	Xy, Llc	Heterogeneous inseminate system
JP4595067B2 (ja)	2002-08-01	2010-12-08	エックスワイ，エルエルシー	低圧精子細胞分離システム
CA2534394C (fr)	2002-08-15	2013-01-08	Xy, Inc.	Cytometre a flux haute resolution
US7169548B2 (en)	2002-09-13	2007-01-30	Xy, Inc.	Sperm cell processing and preservation systems
EP2359689B1 (fr)	2002-09-27	2015-08-26	The General Hospital Corporation	Dispositif microfluidique pour la séparation de cellules et usage du dispositif
CA2445204C (fr)	2002-10-16	2014-08-12	Streck Laboratories, Inc.	Methode et dispositif de prelevement et de conservation de cellules aux fins d'analyse
EP1599608A4 (fr) *	2003-03-05	2007-07-18	Genetic Technologies Ltd	Identification d'adn foetal et de marqueurs de cellule foetale dans du plasma ou du serum maternel
BRPI0408857B1 (pt)	2003-03-28	2018-09-11	Inguran Llc	aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo
ES2541121T3 (es)	2003-05-15	2015-07-16	Xy, Llc	Clasificación eficiente de células haploides por sistemas de citometría de flujo
DK2801363T3 (en)	2004-03-29	2018-05-28	Inguran Llc	PROCEDURE FOR STORING SORTED SPERMATOZOES
CA2574499C (fr)	2004-07-22	2016-11-29	Monsanto Technology Llc	Procede pour enrichir une population de spermatozoides
US7527978B2 (en) *	2004-10-07	2009-05-05	Children's Hospital & Research Center At Oakland	Flow cytometry based micronucleus assays and kits
US7439062B2 (en) *	2004-12-23	2008-10-21	Biocept, Inc.	Beads for capturing target cells from bodily fluid
EP1874920A4 (fr) *	2005-04-05	2009-11-04	Cellpoint Diagnostics	Dispositifs et procédés permettant d'enrichir et de modifier des cellules tumorales circulantes et d'autres particules
US20070196820A1 (en)	2005-04-05	2007-08-23	Ravi Kapur	Devices and methods for enrichment and alteration of cells and other particles
GB0509500D0 (en) *	2005-05-10	2005-06-15	Revealcyte	Method of fetal cell enrichment
EP1886138A4 (fr) *	2005-05-11	2009-04-15	Genetic Technologies Ltd	Procédés d'enrichissement de cellules fétales
US8921102B2 (en)	2005-07-29	2014-12-30	Gpb Scientific, Llc	Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US9424392B2 (en)	2005-11-26	2016-08-23	Natera, Inc.	System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111543B2 (en)	2005-07-29	2021-09-07	Natera, Inc.	System and method for cleaning noisy genetic data and determining chromosome copy number
US10081839B2 (en)	2005-07-29	2018-09-25	Natera, Inc	System and method for cleaning noisy genetic data and determining chromosome copy number
US10083273B2 (en)	2005-07-29	2018-09-25	Natera, Inc.	System and method for cleaning noisy genetic data and determining chromosome copy number
US11111544B2 (en)	2005-07-29	2021-09-07	Natera, Inc.	System and method for cleaning noisy genetic data and determining chromosome copy number
US7618770B2 (en)	2005-07-29	2009-11-17	Xy, Inc.	Methods and apparatus for reducing protein content in sperm cell extenders
WO2007062486A1 (fr) *	2005-12-02	2007-06-07	Simons Haplomics Limited	Procedes de cartographie genetique et d'haplotypage
EP2029779A4 (fr)	2006-06-14	2010-01-20	Living Microsystems Inc	Utilisation de génotypage snp fortement parallèle pour diagnostic fétal
EP2589668A1 (fr)	2006-06-14	2013-05-08	Verinata Health, Inc	Analyse de cellules rares utilisant la division d'échantillons et les marqueurs d'ADN
US8137912B2 (en)	2006-06-14	2012-03-20	The General Hospital Corporation	Methods for the diagnosis of fetal abnormalities
US20080050739A1 (en)	2006-06-14	2008-02-28	Roland Stoughton	Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
GB0616045D0 (en) *	2006-08-11	2006-09-20	Univ Bristol	Blood cell separation
DE102006062619B4 (de) *	2006-12-29	2012-04-26	Medion Diagnostics Ag	Verfahren zur Bestimmung von minoren Zellpopulationen in heterogenen Zellpopulationen
CA2752838A1 (fr) *	2008-02-18	2009-08-27	Genetic Technologies Limited	Procedes de traitement et/ou d'enrichissement de cellules
EP2265942B1 (fr)	2008-03-12	2017-10-18	University Of Virginia Patent Foundation	Détection d'analytes polymères
US20120149587A1 (en) *	2008-03-12	2012-06-14	University Of Virginia Patent Foundation	Method for detecting nucleated cells
EP2321642B1 (fr)	2008-08-04	2017-01-11	Natera, Inc.	Procédés pour une classification d'allèle et une classification de ploïdie
CA3069082C (fr)	2008-09-20	2022-03-22	The Board Of Trustees Of The Leland Stanford Junior University	Diagnostic non effractif d'aneuploidie foetale par sequencage
WO2010078194A1 (fr) *	2008-12-30	2010-07-08	Streck, Inc.	Procédé pour cribler du sang utilisant un conservateur qui peut être sous une forme à l'état sensiblement solide
US11634747B2 (en)	2009-01-21	2023-04-25	Streck Llc	Preservation of fetal nucleic acids in maternal plasma
DE202010018561U1 (de)	2009-01-21	2017-08-28	Streck Inc.	Blutsammelröhrchen
NO2398912T3 (fr)	2009-02-18	2018-02-10
WO2010121294A1 (fr)	2009-04-21	2010-10-28	Genetic Technologies Limited	Procédés d'obtention de matériel génétique foetal
WO2011041485A1 (fr)	2009-09-30	2011-04-07	Gene Security Network, Inc.	Méthode non invasive de détermination d'une ploïdie prénatale
US20110111410A1 (en) *	2009-11-09	2011-05-12	Streck, Inc.	Stabilization of rna in intact cells within a blood sample
US8774488B2 (en)	2010-03-11	2014-07-08	Cellscape Corporation	Method and device for identification of nucleated red blood cells from a maternal blood sample
US11332785B2 (en)	2010-05-18	2022-05-17	Natera, Inc.	Methods for non-invasive prenatal ploidy calling
US10316362B2 (en)	2010-05-18	2019-06-11	Natera, Inc.	Methods for simultaneous amplification of target loci
US9677118B2 (en)	2014-04-21	2017-06-13	Natera, Inc.	Methods for simultaneous amplification of target loci
US11326208B2 (en)	2010-05-18	2022-05-10	Natera, Inc.	Methods for nested PCR amplification of cell-free DNA
US11339429B2 (en)	2010-05-18	2022-05-24	Natera, Inc.	Methods for non-invasive prenatal ploidy calling
US11322224B2 (en)	2010-05-18	2022-05-03	Natera, Inc.	Methods for non-invasive prenatal ploidy calling
US11332793B2 (en)	2010-05-18	2022-05-17	Natera, Inc.	Methods for simultaneous amplification of target loci
WO2011146632A1 (fr)	2010-05-18	2011-11-24	Gene Security Network Inc.	Procédés de classification de ploïdie prénatale non invasive
US20190010543A1 (en)	2010-05-18	2019-01-10	Natera, Inc.	Methods for simultaneous amplification of target loci
US11408031B2 (en)	2010-05-18	2022-08-09	Natera, Inc.	Methods for non-invasive prenatal paternity testing
CN103608466B (zh)	2010-12-22	2020-09-18	纳特拉公司	非侵入性产前亲子鉴定方法
RU2671980C2 (ru)	2011-02-09	2018-11-08	Натера, Инк.	Способы неинвазивного пренатального установления плоидности
US9956281B2 (en)	2011-05-04	2018-05-01	Streck, Inc.	Inactivated virus compositions and methods of preparing such compositions
US10091984B2 (en)	2013-07-24	2018-10-09	Streck, Inc.	Compositions and methods for stabilizing circulating tumor cells
US10577655B2 (en)	2013-09-27	2020-03-03	Natera, Inc.	Cell free DNA diagnostic testing standards
US9499870B2 (en)	2013-09-27	2016-11-22	Natera, Inc.	Cell free DNA diagnostic testing standards
US10262755B2 (en)	2014-04-21	2019-04-16	Natera, Inc.	Detecting cancer mutations and aneuploidy in chromosomal segments
CA2945962C (fr)	2014-04-21	2023-08-29	Natera, Inc.	Detection de mutations et de la ploidie dans des segments chromosomiques
JP2017521091A (ja) *	2014-05-15	2017-08-03	ケルベンクスインコーポレイテッド	診断試験用の胎児有核赤血球（ｎｒｂｃ）の調製
US11168351B2 (en)	2015-03-05	2021-11-09	Streck, Inc.	Stabilization of nucleic acids in urine
US11479812B2 (en)	2015-05-11	2022-10-25	Natera, Inc.	Methods and compositions for determining ploidy
US20170145475A1 (en)	2015-11-20	2017-05-25	Streck, Inc.	Single spin process for blood plasma separation and plasma composition including preservative
WO2018022991A1 (fr)	2016-07-29	2018-02-01	Streck, Inc.	Composition de suspension pour contrôle d'analyse hématologique
WO2018067517A1 (fr)	2016-10-04	2018-04-12	Natera, Inc.	Procédés pour caractériser une variation de nombre de copies à l'aide d'un séquençage de ligature de proximité
GB201618485D0 (en)	2016-11-02	2016-12-14	Ucl Business Plc	Method of detecting tumour recurrence
US10011870B2 (en)	2016-12-07	2018-07-03	Natera, Inc.	Compositions and methods for identifying nucleic acid molecules
CN115119829B (zh)	2017-10-19	2024-07-12	斯特雷克股份有限公司	用于胞外囊泡的溶血和凝血调节以及稳定化的组合物
US12024738B2 (en)	2018-04-14	2024-07-02	Natera, Inc.	Methods for cancer detection and monitoring
US12234509B2 (en)	2018-07-03	2025-02-25	Natera, Inc.	Methods for detection of donor-derived cell-free DNA
US12305235B2 (en)	2019-06-06	2025-05-20	Natera, Inc.	Methods for detecting immune cell DNA and monitoring immune system

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4675286A (en) *	1985-01-28	1987-06-23	Aspen Diagnostics, Inc.	Fetal cell separation and testing
US4835098A (en) *	1985-09-04	1989-05-30	Regents Of University Of Minnesota	Characterization of HLA alleles with locus-specific DNA probes
US4987086A (en) *	1987-11-30	1991-01-22	Becton, Dickinson And Company	Method for analysis of subpopulations of cells
ES2112833T3 (es) *	1988-12-06	1998-04-16	Flinders Technologies Pty Ltd	Aislamiento de celulas fetales a partir de sangre materna para permitir el diagnostico prenatal.
US5153117A (en) *	1990-03-27	1992-10-06	Genetype A.G.	Fetal cell recovery method

1990
- 1990-03-27 US US07/499,932 patent/US5153117A/en not_active Expired - Fee Related
1991
- 1991-03-26 IL IL9767791A patent/IL97677A/en not_active IP Right Cessation
- 1991-03-26 NZ NZ237589A patent/NZ237589A/en unknown
- 1991-03-26 IE IE099691A patent/IE910996A1/en not_active IP Right Cessation
- 1991-03-27 SG SG9607782A patent/SG79188A1/en unknown
- 1991-03-27 DE DE69132269T patent/DE69132269T2/de not_active Expired - Lifetime
- 1991-03-27 DK DK91906073T patent/DK0521909T3/da active
- 1991-03-27 AT AT91906073T patent/ATE194166T1/de not_active IP Right Cessation
- 1991-03-27 ES ES91906073T patent/ES2149760T3/es not_active Expired - Lifetime
- 1991-03-27 WO PCT/AU1991/000115 patent/WO1991014768A1/fr not_active Ceased
- 1991-03-27 JP JP3505625A patent/JP2965699B2/ja not_active Expired - Lifetime
- 1991-03-27 ZA ZA912317A patent/ZA912317B/xx unknown
- 1991-03-27 US US07/927,313 patent/US5447842A/en not_active Expired - Lifetime
- 1991-03-27 CA CA002059554A patent/CA2059554C/fr not_active Expired - Lifetime
- 1991-03-27 EP EP91906073A patent/EP0521909B1/fr not_active Expired - Lifetime
- 1991-03-27 AU AU74716/91A patent/AU649027B2/en not_active Expired
2000
- 2000-09-26 GR GR20000402176T patent/GR3034487T3/el not_active IP Right Cessation

Also Published As

Publication number	Publication date
ES2149760T3 (es)	2000-11-16
AU7471691A (en)	1991-10-21
SG79188A1 (en)	2001-03-20
ZA912317B (en)	1991-12-24
NZ237589A (en)	1992-04-28
AU649027B2 (en)	1994-05-12
JPH05506144A (ja)	1993-09-16
EP0521909B1 (fr)	2000-06-28
DE69132269T2 (de)	2001-03-15
GR3034487T3 (en)	2000-12-29
DE69132269D1 (de)	2000-08-03
ATE194166T1 (de)	2000-07-15
WO1991014768A1 (fr)	1991-10-03
EP0521909A4 (en)	1993-11-18
IE910996A1 (en)	1991-10-09
CA2059554A1 (fr)	1991-09-28
EP0521909A1 (fr)	1993-01-13
IL97677A0 (en)	1992-06-21
US5153117A (en)	1992-10-06
JP2965699B2 (ja)	1999-10-18
DK0521909T3 (da)	2000-10-30
CA2059554C (fr)	2004-06-22
US5447842A (en)	1995-09-05

Legal Events

Date	Code	Title
1996-12-05	FF	Patent granted
1997-02-18	KB	Patent renewed
1997-08-14	KB	Patent renewed
2001-07-24	KB	Patent renewed
2005-05-17	KB	Patent renewed
2011-12-29	EXP	Patent expired

Publication	Publication Date	Title
US5447842A (en)	1995-09-05	Fetal cell recovery method
Bianchi et al.	1993	Erythroid‐specific antibodies enhance detection of fetal nucleated erythrocytes in maternal blood
US5437987A (en)	1995-08-01	Triple gradient process with antibody panning to recover nucleated fetal cells from maternal blood
US5714325A (en)	1998-02-03	Prenatal diagnosis by isolation of fetal granulocytes from maternal blood
EP0500727B1 (fr)	1998-01-21	Procede non envahissant d'isolation et de detection d'adn f tal
EP1007965B1 (fr)	2005-03-02	Utilisation d'anticorps diriges contre l'hemoglobine embryonnaire pour identifier des cellules foetales
US5962234A (en)	1999-10-05	Use of anti-embryonic epsilon hemoglobin antibodies to identify fetal cells
US5374531A (en)	1994-12-20	Immunoassay for determination of cells
US5385822A (en)	1995-01-31	Methods for detection and quantification of cell subsets within subpopulations of a mixed cell population
US5270169A (en)	1993-12-14	Detection of HLA antigen-containing immune complexes
US20030022245A1 (en)	2003-01-30	Method and compositions for analysis of pentraxin receptors as indicators of disease
WO1994025873A1 (fr)	1994-11-10	Procedes d'enrichissement de cellules souches f×tales provenant du sang maternel
AU691040B2 (en)	1998-05-07	Enrichment of fetal cells from maternal blood
US4797475A (en)	1989-01-10	Method and composition for isolating white cell elements
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EP0127358A2 (fr)	1984-12-05	Méthode et composition pour isoler les éléments des globules blancs
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