IT1250936B - Sistema di controllo automatico per elettroforesi. - Google Patents

Sistema di controllo automatico per elettroforesi.

Info

Publication number
IT1250936B
IT1250936B ITRM910741A ITRM910741A IT1250936B IT 1250936 B IT1250936 B IT 1250936B IT RM910741 A ITRM910741 A IT RM910741A IT RM910741 A ITRM910741 A IT RM910741A IT 1250936 B IT1250936 B IT 1250936B
Authority
IT
Italy
Prior art keywords
voltage gradient
electrophoresis
macromolecules
switch time
control system
Prior art date
Application number
ITRM910741A
Other languages
English (en)
Inventor
Eric Lai
Bruce W Birren
Will H Stubblebine
George E Bers
Original Assignee
Bio Rad Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories filed Critical Bio Rad Laboratories
Publication of ITRM910741A0 publication Critical patent/ITRM910741A0/it
Publication of ITRM910741A1 publication Critical patent/ITRM910741A1/it
Application granted granted Critical
Publication of IT1250936B publication Critical patent/IT1250936B/it

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Engineering & Computer Science (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Viene illustrato un sistema per migliorare la separazione per via elettroforetica di macromolecole con l'aiuto di un calcolatore. Il sistema conserva una base di conoscenza ricavata da dati di calibrazione ottenuti separando macromolecole, in particolare DNA, di grandezza nota sotto una varietà di condizioni pianificate scelte per ottimizzare le separazioni. L'utilizzatore imposta le grandezze di DNA più piccole e più grandi che debbono essere risolte. Da questa informazione, sistema regola automaticamente il gradiente di tensione ottimale, Voltage Gradient, Angle, Initial Switch Time, Final Switch Time, Forward Voltage Gradient, e Reverse Voltage Gradient sotto forma di parametri, aumentando in tal modo la risoluzione della elettroforesi.
ITRM910741A 1990-10-02 1991-10-02 Sistema di controllo automatico per elettroforesi. IT1250936B (it)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US59158990A 1990-10-02 1990-10-02

Publications (3)

Publication Number Publication Date
ITRM910741A0 ITRM910741A0 (it) 1991-10-02
ITRM910741A1 ITRM910741A1 (it) 1993-04-02
IT1250936B true IT1250936B (it) 1995-04-22

Family

ID=24367064

Family Applications (1)

Application Number Title Priority Date Filing Date
ITRM910741A IT1250936B (it) 1990-10-02 1991-10-02 Sistema di controllo automatico per elettroforesi.

Country Status (6)

Country Link
JP (1) JPH04264254A (it)
CA (1) CA2052037A1 (it)
DE (1) DE4132694A1 (it)
FR (1) FR2670683B1 (it)
GB (1) GB2249395B (it)
IT (1) IT1250936B (it)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7077328B2 (en) * 1998-07-31 2006-07-18 Abbott Laboratories Analyte test instrument system including data management system
AU6574601A (en) * 2000-06-07 2001-12-17 Centro Nacional De Investigaciones Cientificas (Cnic) Pulsed field electrophoresis chambers, accessories and method of utilization for seperation of DNA molecules
CU23095A1 (es) 2000-11-07 2005-11-18 Cnic Ct Nac Investigaciones Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US9186685B2 (en) 2012-01-13 2015-11-17 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
EP1720636A4 (en) 2004-02-02 2012-06-20 Univ British Columbia SCODAPHORESIS AND METHOD AND DEVICES FOR MOVING AND CONCENTRATING PARTICLES
CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
JP4732406B2 (ja) * 2007-08-03 2011-07-27 株式会社堀場製作所 水質測定装置
US8475641B2 (en) 2008-02-01 2013-07-02 The University Of British Columbia Methods and apparatus for particle introduction and recovery
US8877028B2 (en) 2009-04-21 2014-11-04 The University Of British Columbia System and methods for detection of particles
CA2742327A1 (en) 2011-05-20 2012-11-20 The University Of British Columiba Systems and methods for enhanced scoda
WO2013166444A2 (en) 2012-05-04 2013-11-07 Boreal Genomics Corp. Biomarker analysis using scodaphoresis
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
WO2016185284A1 (en) 2015-05-20 2016-11-24 Boreal Genomics, Inc. Method for isolating target nucleic acid using heteroduplex binding proteins
US12011716B2 (en) 2019-10-29 2024-06-18 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4771384A (en) * 1986-07-24 1988-09-13 Dnastar, Inc. System and method for fragmentation mapping
JPH02176457A (ja) * 1988-09-15 1990-07-09 Carnegie Inst Of Washington パルス志向電気泳動
CA2012379C (en) * 1989-04-24 2000-01-25 Gary W. Slater Processes for the preparation and separation of macromolecules

Also Published As

Publication number Publication date
DE4132694A1 (de) 1992-04-16
JPH04264254A (ja) 1992-09-21
ITRM910741A0 (it) 1991-10-02
GB9120922D0 (en) 1991-11-13
GB2249395B (en) 1994-08-10
ITRM910741A1 (it) 1993-04-02
FR2670683B1 (fr) 1993-07-09
FR2670683A1 (fr) 1992-06-26
CA2052037A1 (en) 1992-04-03
GB2249395A (en) 1992-05-06

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Legal Events

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0001 Granted