IT1251435B - Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono. - Google Patents

Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.

Info

Publication number
IT1251435B
IT1251435B ITMI912349A ITMI912349A IT1251435B IT 1251435 B IT1251435 B IT 1251435B IT MI912349 A ITMI912349 A IT MI912349A IT MI912349 A ITMI912349 A IT MI912349A IT 1251435 B IT1251435 B IT 1251435B
Authority
IT
Italy
Prior art keywords
plasmids
contain
dna sequences
gene
kluyveromyces lactis
Prior art date
Application number
ITMI912349A
Other languages
English (en)
Inventor
Cesira Luigia Galeotti
Eugenia Gallo
Gian Maria Rossolini
Maria Letizia Riccio
Maria Cristina Thaller
Original Assignee
Sclavo Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sclavo Spa filed Critical Sclavo Spa
Priority to ITMI912349A priority Critical patent/IT1251435B/it
Publication of ITMI912349A0 publication Critical patent/ITMI912349A0/it
Priority to EP92114838A priority patent/EP0537456A1/en
Priority to CA002077489A priority patent/CA2077489A1/en
Publication of ITMI912349A1 publication Critical patent/ITMI912349A1/it
Application granted granted Critical
Publication of IT1251435B publication Critical patent/IT1251435B/it

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

E' descritto un vettore genetico per l'integrazione stabile, per ricombinazione omologa, di specifiche sequenze di DNA nel genoma dei lieviti Kluyveromyces lactis e Saccharonmyces cerevisiae che consente di ottenere eventi integrativi multipli in numero elevato e i plasmidi che lo contengono.
ITMI912349A 1991-09-04 1991-09-04 Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono. IT1251435B (it)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ITMI912349A IT1251435B (it) 1991-09-04 1991-09-04 Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.
EP92114838A EP0537456A1 (en) 1991-09-04 1992-08-31 Genetic vector for multiple stable integration of DNA sequences into the genome of the yeasts kluyveromyces lactis and saccharomyces cerevisiae and plasmids containing the same
CA002077489A CA2077489A1 (en) 1991-09-04 1992-09-03 Genetic vector for multiple stable integration of dna sequences into the genome of the yeasts kluyveromyces lactis and saccharomyces cerevisiae and plasmids containing the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI912349A IT1251435B (it) 1991-09-04 1991-09-04 Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.

Publications (3)

Publication Number Publication Date
ITMI912349A0 ITMI912349A0 (it) 1991-09-04
ITMI912349A1 ITMI912349A1 (it) 1993-03-04
IT1251435B true IT1251435B (it) 1995-05-09

Family

ID=11360611

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI912349A IT1251435B (it) 1991-09-04 1991-09-04 Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.

Country Status (3)

Country Link
EP (1) EP0537456A1 (it)
CA (1) CA2077489A1 (it)
IT (1) IT1251435B (it)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6107057A (en) * 1995-09-25 2000-08-22 Chiron Corporation Pichia secretory leader for protein expression
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
EP0973918B1 (en) * 1997-04-11 2008-06-11 DSM IP Assets B.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal rna encoding domain, particularly with kluyveromyces
EP1318200A3 (en) 2001-12-07 2004-04-07 Daicel Chemical Industries, Ltd. Methods for producing optically active alcohols
CN100572535C (zh) 2002-10-22 2009-12-23 卫材R&D管理有限公司 在分裂停止后的产多巴胺型神经元前体细胞中特异性表达的基因
WO2008041385A1 (en) 2006-09-29 2008-04-10 National University Corporation NARA Institute of Science and Technology Novel alkane polyol dehydrogenase
EP2023143A1 (en) 2007-08-06 2009-02-11 Boehringer Ingelheim Vetmedica Gmbh Immunogenic streptococcus proteins
US20110262977A1 (en) 2008-09-01 2011-10-27 Daicel Chemical Industries, Ltd. Process for production of optically active amine derivative
EP2330190A1 (en) 2008-09-01 2011-06-08 Daicel Chemical Industries, Ltd. Process for production of optically active amine derivative
WO2011052718A1 (ja) 2009-10-30 2011-05-05 ダイセル化学工業株式会社 １，３－ブタンジオール生産機能を付与された遺伝子組換え微生物及びその利用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1196484B (it) * 1986-07-11 1988-11-16 Sclavo Spa Vettore ad espressione e secrezione in lieviti,utile per la preparazione di proteine eterologhe
NO302485B1 (no) * 1987-07-28 1998-03-09 Gist Brocades Bv Fremgangsmåte for fremstilling av et polypeptid av interesse i en Klyveromyces vertscelle, transformert Klyveromyces vertscelle og anvendelse derav, plasmid og DNA konstruksjon
ATE160176T1 (de) * 1989-07-07 1997-11-15 Unilever Nv Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes

Also Published As

Publication number Publication date
ITMI912349A1 (it) 1993-03-04
ITMI912349A0 (it) 1991-09-04
CA2077489A1 (en) 1993-03-05
EP0537456A1 (en) 1993-04-21

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