ATE160176T1 - Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes - Google Patents
Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzesInfo
- Publication number
- ATE160176T1 ATE160176T1 AT90911878T AT90911878T ATE160176T1 AT E160176 T1 ATE160176 T1 AT E160176T1 AT 90911878 T AT90911878 T AT 90911878T AT 90911878 T AT90911878 T AT 90911878T AT E160176 T1 ATE160176 T1 AT E160176T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- expression vector
- integration
- producing
- yeast
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 230000010354 integration Effects 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 239000013604 expression vector Substances 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 title 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 2
- 241000228212 Aspergillus Species 0.000 abstract 1
- 244000303965 Cyamopsis psoralioides Species 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 241000206602 Eukaryota Species 0.000 abstract 1
- 241000235649 Kluyveromyces Species 0.000 abstract 1
- 102000004882 Lipase Human genes 0.000 abstract 1
- 108090001060 Lipase Proteins 0.000 abstract 1
- 239000004367 Lipase Substances 0.000 abstract 1
- 102000004316 Oxidoreductases Human genes 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 241000235648 Pichia Species 0.000 abstract 1
- 241000235527 Rhizopus Species 0.000 abstract 1
- 108020001027 Ribosomal DNA Proteins 0.000 abstract 1
- 241000235070 Saccharomyces Species 0.000 abstract 1
- 241000223259 Trichoderma Species 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 235000019421 lipase Nutrition 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB898915659A GB8915659D0 (en) | 1989-07-07 | 1989-07-07 | Enzymes,their production and use |
| EP90201007 | 1990-04-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE160176T1 true ATE160176T1 (de) | 1997-11-15 |
Family
ID=26125849
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97200631T ATE196505T1 (de) | 1989-07-07 | 1990-07-09 | Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopie-integrierung eines expressionsvektors tranformierten pilzes |
| AT90911878T ATE160176T1 (de) | 1989-07-07 | 1990-07-09 | Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97200631T ATE196505T1 (de) | 1989-07-07 | 1990-07-09 | Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopie-integrierung eines expressionsvektors tranformierten pilzes |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6090574A (de) |
| EP (2) | EP0481008B1 (de) |
| JP (1) | JP3273609B2 (de) |
| AT (2) | ATE196505T1 (de) |
| CA (1) | CA2063592C (de) |
| DE (2) | DE69031710T2 (de) |
| DK (2) | DK0778348T3 (de) |
| ES (2) | ES2109238T3 (de) |
| IE (1) | IE81016B1 (de) |
| WO (1) | WO1991000920A2 (de) |
Families Citing this family (106)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991014781A1 (en) * | 1990-03-19 | 1991-10-03 | Henkel Research Corporation | METHOD FOR INCREASING THE OMEGA-HYDROXYLASE ACTIVITY IN $i(CANDIDA TROPICALIS) |
| ATE140974T1 (de) * | 1990-10-04 | 1996-08-15 | Japan Maize Prod | Wirt-vektor-system |
| JP3133774B2 (ja) * | 1990-10-04 | 2001-02-13 | 日本食品化工株式会社 | 新規な宿主−ベクター系 |
| IT1251435B (it) * | 1991-09-04 | 1995-05-09 | Sclavo Spa | Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono. |
| AU5811194A (en) * | 1992-12-11 | 1994-07-04 | Quest International B.V. | The use of the (kluyveromyces marxianus) inulinase gene promoter for protein production |
| US5786212A (en) * | 1993-09-02 | 1998-07-28 | Pfizer Inc. | Multiple integrative vectors and Yarrowia lipolytica transformants |
| US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
| JP2001501089A (ja) * | 1996-09-24 | 2001-01-30 | バハマイア，アンドレアス | Ｄｎａ構築物及びこれらのｄｎａ構築物を用いてタンパク質を生産するための方法 |
| US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| JP4307563B2 (ja) | 1997-04-07 | 2009-08-05 | ユニリーバー・ナームローゼ・ベンノートシャープ | 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換 |
| EP0870835A1 (de) * | 1997-04-07 | 1998-10-14 | Unilever N.V. | Transformation von Schimmeln durch Agrobacterium, insbesondere von der Gattung Aspergillus |
| ES2168236T3 (es) | 1997-04-09 | 2005-04-16 | Danisco A/S | Utilizacion de lipasa para mejorar las pastas de pan y los productos de panaderia. |
| JP5303084B2 (ja) | 1997-04-11 | 2013-10-02 | コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ | 工業的組み換え生物を溝築するための手段としての遺伝子変換 |
| ATE398181T1 (de) * | 1997-04-11 | 2008-07-15 | Dsm Ip Assets Bv | Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces |
| US6265186B1 (en) | 1997-04-11 | 2001-07-24 | Dsm N.V. | Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces |
| DE69732378D1 (de) * | 1997-05-27 | 2005-03-03 | Hanil Synthetic Fiber Co Ltd | Verfahren zur herstellung von rekombinanten eiweissen durch verwendung von hocheffizienten expressionsvektoren aus saccharomyces cerevisiae |
| US6228583B1 (en) * | 1997-08-04 | 2001-05-08 | Massachusetts Institute Of Technology | Assays for compounds which extend life span |
| EP1854890A1 (de) * | 1997-12-22 | 2007-11-14 | DSM IP Assets B.V. | Expressionsklonierung in filamentösen Pilzen |
| US6436643B1 (en) | 1997-12-22 | 2002-08-20 | Unilever Patent Holdings Bv | Process for site-directed integration of multiple copies of a gene in a mould |
| EP1073755A2 (de) * | 1998-04-28 | 2001-02-07 | Axys Pharmaceuticals, Inc. | Serinprotease mit der fähigkeit das bindungsprotein des insulinähnlichen wachstumsfaktor selektiv zu spalten |
| KR100618495B1 (ko) | 1998-10-06 | 2006-08-31 | 마크 아론 에말파브 | 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서 |
| US7220542B2 (en) | 2000-07-17 | 2007-05-22 | Van Den Brink Johannes Maarten | Expression cloning in filamentous fungi |
| WO2001020007A1 (en) * | 1999-09-13 | 2001-03-22 | Concordia University | A multifunctional system for the efficient manipulation of protein expression in filamentous fungi and method using same |
| DE19963690A1 (de) * | 1999-11-23 | 2001-06-13 | Rhein Biotech Proz & Prod Gmbh | Vektoren und Verfahren zur Herstellung rekombinanter Proteine in Pilzen |
| CN1524120A (zh) * | 2000-11-15 | 2004-08-25 | 凯敏工业公司 | 超量产酶的转基因微生物 |
| CZ303983B6 (cs) * | 2000-12-13 | 2013-07-31 | Unilever N. V. | Zpusob výroby heterologního proteinu |
| WO2002059297A2 (en) | 2001-01-25 | 2002-08-01 | Evolva Biotech A/S | A library of a collection of cells |
| WO2002059330A2 (en) * | 2001-01-25 | 2002-08-01 | Evolva Biotech A/S | Artificial chromosomes comprising concatemers of expressible nucleotide sequences |
| BR0209154A (pt) | 2001-05-18 | 2004-07-20 | Danisco | Processo de preparação de uma massa com uma enzima |
| ITMI20011728A1 (it) * | 2001-08-06 | 2003-02-06 | Consorzio Per Le Ricerche E Lo | Vettore per l'integrazione sito-specifica di sequenze di dna eterologhe in lieviti metilotrofi |
| DE602004030000D1 (de) | 2003-01-17 | 2010-12-23 | Danisco | Verfahren zur in-situ-herstellung eines emulgators in einem nahrungsmittel |
| WO2004072225A2 (en) * | 2003-02-12 | 2004-08-26 | Ramot At Tel Aviv University Ltd. | Transgenic fungi expressing bcl-2 and methods of using bcl-2 or portions thereof for improving biomass production, survival, longevity, stress resistance and pathogenicity of fungi |
| GB0405637D0 (en) | 2004-03-12 | 2004-04-21 | Danisco | Protein |
| CN101052702B (zh) | 2004-07-16 | 2013-01-09 | 杜邦营养生物科学有限公司 | 脂肪分解酶及其在食品工业中的应用 |
| EP2505651A3 (de) | 2006-12-10 | 2013-01-09 | Dyadic International, Inc. | Pilzisolate mit reduzierter Proteaseaktivität |
| US8551751B2 (en) | 2007-09-07 | 2013-10-08 | Dyadic International, Inc. | BX11 enzymes having xylosidase activity |
| WO2009065777A1 (en) | 2007-11-20 | 2009-05-28 | Dsm Ip Assets B.V. | Dicarboxylic acid production in a filamentous fungus |
| ATE555203T1 (de) | 2007-11-20 | 2012-05-15 | Dsm Ip Assets Bv | Dicarbonsäureproduktion in eukaryonten |
| BRPI0819717B1 (pt) | 2007-11-20 | 2022-05-17 | Dsm Ip Assets B.V. | Levedura recombinante e processo para a produção de ácido succínico |
| EP2116136A1 (de) | 2008-05-08 | 2009-11-11 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Neue Phytasen |
| BRPI0913818A2 (pt) | 2008-10-03 | 2017-03-28 | Agrisoma Biosciences Inc | método de produção de plantas transgênicas, planta transgênica e método de produção de óleo |
| US8735100B2 (en) | 2009-03-17 | 2014-05-27 | Nederlandse Organisatie Voor Toegepast -Natuurwetenschappelijk Onderzoek Tno | Cellulose and ligno-cellulose active proteins |
| WO2010118932A1 (en) | 2009-04-15 | 2010-10-21 | Dsm Ip Assets B.V. | Dicarboxylic acid production process |
| RU2439157C2 (ru) * | 2009-06-18 | 2012-01-10 | Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП ГосНИИгенетика) | ГЕНЕТИЧЕСКАЯ КОНСТРУКЦИЯ ДЛЯ ПОЛУЧЕНИЯ СТАБИЛЬНЫХ ТРАНСФОРМАНТОВ ГРИБОВ РОДА Rhizopus |
| UA108853C2 (uk) | 2009-07-10 | 2015-06-25 | Спосіб ферментації галактози | |
| DE102009035671A1 (de) * | 2009-07-30 | 2011-02-03 | Dechema Gesellschaft Für Chemische Technik Und Biotechnologie E.V. | Verfahren zur Expression von homologen Proteinen und/oder Peptiden in Pilzen der Klasse Dothideomycetes |
| RU2420567C1 (ru) * | 2009-12-09 | 2011-06-10 | Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП ГосНИИгенетика) | СПОСОБ КОНСТРУИРОВАНИЯ ШТАММОВ ДРОЖЖЕЙ Saccharomyces cerevisiae - СТАБИЛЬНЫХ ПРОДУЦЕНТОВ СОМАТОТРОПИНА ЧЕЛОВЕКА, ШТАММ ДРОЖЖЕЙ Saccharomyces cerevisiae - СТАБИЛЬНЫЙ ПРОДУЦЕНТ СОМАТОТРОПИНА ЧЕЛОВЕКА (ВАРИАНТЫ) |
| AR081827A1 (es) | 2010-04-21 | 2012-10-24 | Dsm Ip Assets Bv | Proceso para producir celulas capaces de convertir arabinosa |
| EP2388331A1 (de) | 2010-05-21 | 2011-11-23 | Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO | Konstitutiver Promoter |
| CN103154234B (zh) | 2010-10-13 | 2016-08-17 | 帝斯曼知识产权资产管理有限公司 | 发酵戊糖和葡萄糖的酵母细胞 |
| EP2699589A2 (de) | 2011-04-22 | 2014-02-26 | DSM IP Assets B.V. | Hefezelle mit fähigkeit zur umwandlung von zuckern, einschliesslich arabinose und xylose |
| EP2554668A1 (de) | 2011-08-04 | 2013-02-06 | DSM IP Assets B.V. | Zelle zur Fermentierung von Pentosezucker |
| AR087423A1 (es) | 2011-08-04 | 2014-03-19 | Dsm Ip Assets Bv | Celula capaz de fermentar azucares pentosas |
| AU2013211605B2 (en) | 2012-01-23 | 2017-04-06 | Dsm Ip Assets B.V. | Diterpene production |
| US20130273619A1 (en) | 2012-04-16 | 2013-10-17 | Basf Se | Process for the Preparation of (3E, 7E)-Homofarnesol |
| US10273507B2 (en) | 2012-10-16 | 2019-04-30 | Dsm Ip Assets B.V. | Cells with improved pentose conversion |
| EP2772545A1 (de) | 2013-03-01 | 2014-09-03 | Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO | Stärkeaktive Proteine |
| MX2015015274A (es) | 2013-05-02 | 2016-05-26 | Dutch Dna Biotech B V | Ruta de ácido organico novedosa. |
| US10689681B2 (en) | 2013-05-31 | 2020-06-23 | Dsm Ip Assets B.V. | Microorganisms for diterpene production |
| EP3021689B1 (de) | 2013-07-15 | 2021-03-24 | DSM IP Assets B.V. | Herstellung von diterpen |
| AU2014298420B2 (en) | 2013-07-31 | 2018-10-04 | Dsm Ip Assets B.V. | Recovery of steviol glycosides |
| CN107429273A (zh) | 2015-02-16 | 2017-12-01 | 帝斯曼知识产权资产管理有限公司 | 用于在厌氧条件下生产衣康酸的方法 |
| EP3718417B1 (de) | 2015-04-03 | 2025-09-03 | DSM IP Assets B.V. | Steviol-glycoside |
| US11332724B2 (en) | 2015-04-21 | 2022-05-17 | Dsm Ip Assets B.V. | Microbial production of terpenoids |
| US11319543B2 (en) | 2015-05-22 | 2022-05-03 | Dsm Ip Assets B.V. | Acetate consuming yeast cell |
| BR112018000550B1 (pt) | 2015-07-10 | 2022-05-10 | Dsm Ip Assets B.V. | Método para preparar uma composição de glicosídeo de esteviol |
| US20180230505A1 (en) | 2015-08-13 | 2018-08-16 | Dsm Ip Assets B.V. | Steviol glycoside transport |
| WO2017050652A1 (en) | 2015-09-25 | 2017-03-30 | Dsm Ip Assets B.V. | Asparaginase |
| WO2017060195A1 (en) | 2015-10-06 | 2017-04-13 | Dsm Ip Assets B.V. | Eukaryotic cell with increased production of fermentation product |
| WO2017167847A1 (en) | 2016-03-31 | 2017-10-05 | Dsm Ip Assets B.V. | Production of milk with low lactose content |
| WO2017167848A1 (en) | 2016-03-31 | 2017-10-05 | Dsm Ip Assets B.V. | Enzyme composition and preparation of a dairy product with improved properties |
| WO2017167849A1 (en) | 2016-03-31 | 2017-10-05 | Dsm Ip Assets B.V. | Enzyme composition and preparation of a dairy product with improved properties |
| CN109312296B (zh) | 2016-06-14 | 2023-05-05 | 帝斯曼知识产权资产管理有限公司 | 重组酵母细胞 |
| CN109689864B (zh) | 2016-07-13 | 2023-04-04 | 帝斯曼知识产权资产管理有限公司 | 苹果酸脱氢酶 |
| BR112019002432A2 (pt) | 2016-08-09 | 2019-06-04 | Dsm Ip Assets Bv | cristalização de glicosídeos de esteviol |
| BR112019002417B1 (pt) | 2016-08-09 | 2023-05-02 | Dsm Ip Assets B.V. | Cristalização de glicosídeos de esteviol |
| CN110036114B (zh) | 2016-08-26 | 2023-09-29 | 勒萨弗尔公司 | 提高的衣康酸生产 |
| WO2018073107A1 (en) | 2016-10-19 | 2018-04-26 | Dsm Ip Assets B.V. | Eukaryotic cell comprising xylose isomerase |
| WO2018114938A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114912A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114940A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114941A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114995A1 (en) | 2016-12-22 | 2018-06-28 | Dsm Ip Assets B.V. | Fermentation process for producing steviol glycosides |
| EP3638770B1 (de) | 2017-06-13 | 2023-01-18 | DSM IP Assets B.V. | Rekombinante hefezelle |
| BR112019027919A2 (pt) * | 2017-06-30 | 2020-07-21 | Ptt Global Chemical Public Company Limited | micro-organismo com número de cópias estabilizado de sequência de dna funcional e métodos associados |
| EP3688177A1 (de) | 2017-09-26 | 2020-08-05 | DSM IP Assets B.V. | Essigsäureverbrauchender stamm |
| EP3533878A1 (de) | 2018-02-28 | 2019-09-04 | Dutch DNA Biotech B.V. | Verfahren zur herstellung von 2-hydroxy-2-methylbutandisäure unter verwendung von aspergillus |
| BR112020025589A2 (pt) | 2018-06-19 | 2021-03-23 | Dsm Ip Assets B.V. | variantes de enzima lipolítica |
| CN111254128A (zh) * | 2018-11-30 | 2020-06-09 | 康码（上海）生物科技有限公司 | 蛋白激酶a催化亚基tpk1基因改造方法及其应用 |
| CN111254127B (zh) * | 2018-11-30 | 2022-06-10 | 康码（上海）生物科技有限公司 | 蛋白激酶a催化亚基tpk2基因改造方法及其应用 |
| AU2021367159A1 (en) | 2020-10-22 | 2023-05-04 | Cargill, Incorporated | Microorganisms for diterpene production |
| FR3122436B1 (fr) * | 2021-04-30 | 2025-04-11 | Ifp Energies Now | Insertion multicopies d’un gène d’intérêt dans le génome d’un champignon |
| AU2022297881A1 (en) | 2021-06-24 | 2024-01-18 | Fonterra Co-Operative Group Limited | Recombinant beta-lactoglobulin |
| CN117916381A (zh) | 2021-07-12 | 2024-04-19 | 丹尼斯科美国公司 | 重组酵母细胞 |
| WO2023285297A1 (en) | 2021-07-12 | 2023-01-19 | Dsm Ip Assets B.V. | Recombinant yeast cell |
| MX2024000606A (es) | 2021-07-12 | 2024-01-30 | Danisco Us Inc | Celula de levadura recombinante. |
| US20250283121A1 (en) | 2021-07-12 | 2025-09-11 | Danisco Us Inc. | Recombinant yeast cell |
| WO2023285282A1 (en) | 2021-07-12 | 2023-01-19 | Dsm Ip Assets B.V. | Recombinant yeast cell |
| WO2023285280A1 (en) | 2021-07-12 | 2023-01-19 | Dsm Ip Assets B.V. | Recombinant yeast cell |
| CN113564206B (zh) * | 2021-07-29 | 2023-09-26 | 山东大学 | 一种目的基因多拷贝整合到酿酒酵母染色体rDNA的方法 |
| WO2023079050A1 (en) | 2021-11-04 | 2023-05-11 | Dsm Ip Assets B.V. | Recombinant yeast cell |
| WO2023079048A1 (en) | 2021-11-04 | 2023-05-11 | Dsm Ip Assets B.V. | Process for the production of ethanol and recombinant yeast cell |
| US20240409965A1 (en) | 2021-11-04 | 2024-12-12 | Danisco Us Inc. | Variant polypeptide and recombinant yeast cell |
| EP4430174A1 (de) | 2021-11-09 | 2024-09-18 | Amgen Inc. | Herstellung von therapeutischen proteinen |
| US20250304938A1 (en) | 2022-05-16 | 2025-10-02 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2025012325A1 (en) | 2023-07-11 | 2025-01-16 | Dsm Ip Assets B.V. | Protein arginine deiminase |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU600885B2 (en) * | 1984-05-25 | 1990-08-30 | Zymogenetics Inc. | Stable DNA constructs |
| GB8415186D0 (en) * | 1984-06-14 | 1984-07-18 | Ciba Geigy Ag | Polypeptides |
| CA1341130C (en) * | 1984-07-27 | 2000-10-31 | Adrianus Marinus Ledeboer | Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology |
| JPS61134325A (ja) * | 1984-12-04 | 1986-06-21 | Teijin Ltd | ハイブリツド抗体遺伝子の発現方法 |
| JPH0614865B2 (ja) * | 1985-12-13 | 1994-03-02 | 麒麟麦酒株式会社 | α―アセト乳酸脱炭酸酵素をコードするＤＮＡ鎖およびこのＤＮＡ鎖により形質転換された酵母 |
| KR910002604B1 (ko) | 1986-05-28 | 1991-04-27 | 미쓰비시덴기 가부시기가이샤 | 영상 재생 장치 |
| CA1339101C (en) * | 1986-06-03 | 1997-07-29 | Nicolaas Overbeeke | Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods |
| GB8629536D0 (en) | 1986-12-10 | 1987-01-21 | Unilever Plc | Enzymatic detergent composition |
| PT88556B (pt) * | 1987-09-28 | 1992-11-30 | Smithkline Biolog | Processo de integracao genomica estavel e de expressao de uma sequencia de cadificacao estranha, em leveduras |
| DK571587D0 (da) | 1987-11-02 | 1987-11-02 | Novo Industri As | Enzymatisk detergentsammensaetning |
| EP0318775B1 (de) * | 1987-12-03 | 1994-02-02 | Chisso Corporation | Lipasegen |
| US5001230A (en) * | 1988-02-18 | 1991-03-19 | Schering Corporation | T cell activation markers |
| JP3079276B2 (ja) * | 1988-02-28 | 2000-08-21 | 天野製薬株式会社 | 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法 |
| JP2639677B2 (ja) * | 1988-03-14 | 1997-08-13 | 旭化成工業株式会社 | リパーゼの遺伝情報を有するｄｎａ |
-
1990
- 1990-07-09 WO PCT/EP1990/001138 patent/WO1991000920A2/en not_active Ceased
- 1990-07-09 ES ES90911878T patent/ES2109238T3/es not_active Expired - Lifetime
- 1990-07-09 JP JP51119190A patent/JP3273609B2/ja not_active Expired - Fee Related
- 1990-07-09 DK DK97200631T patent/DK0778348T3/da active
- 1990-07-09 AT AT97200631T patent/ATE196505T1/de not_active IP Right Cessation
- 1990-07-09 CA CA002063592A patent/CA2063592C/en not_active Expired - Lifetime
- 1990-07-09 AT AT90911878T patent/ATE160176T1/de not_active IP Right Cessation
- 1990-07-09 EP EP90911878A patent/EP0481008B1/de not_active Expired - Lifetime
- 1990-07-09 ES ES97200631T patent/ES2150188T3/es not_active Expired - Lifetime
- 1990-07-09 EP EP97200631A patent/EP0778348B1/de not_active Expired - Lifetime
- 1990-07-09 DE DE69031710T patent/DE69031710T2/de not_active Expired - Lifetime
- 1990-07-09 DK DK90911878.8T patent/DK0481008T3/da active
- 1990-07-09 IE IE249690A patent/IE81016B1/en not_active IP Right Cessation
- 1990-07-09 DE DE69033633T patent/DE69033633T2/de not_active Expired - Lifetime
-
1994
- 1994-12-27 US US08/364,010 patent/US6090574A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES2150188T3 (es) | 2000-11-16 |
| EP0481008A1 (de) | 1992-04-22 |
| EP0778348A1 (de) | 1997-06-11 |
| DE69031710D1 (de) | 1997-12-18 |
| JPH05501949A (ja) | 1993-04-15 |
| CA2063592C (en) | 2003-01-21 |
| DK0778348T3 (da) | 2000-12-04 |
| ATE196505T1 (de) | 2000-10-15 |
| DE69033633D1 (de) | 2000-10-26 |
| WO1991000920A2 (en) | 1991-01-24 |
| IE81016B1 (en) | 1999-09-22 |
| ES2109238T3 (es) | 1998-01-16 |
| IE902496A1 (en) | 1991-02-13 |
| DE69031710T2 (de) | 1998-03-05 |
| EP0481008B1 (de) | 1997-11-12 |
| DK0481008T3 (da) | 1998-05-04 |
| JP3273609B2 (ja) | 2002-04-08 |
| US6090574A (en) | 2000-07-18 |
| WO1991000920A3 (en) | 1991-03-07 |
| CA2063592A1 (en) | 1991-01-08 |
| EP0778348B1 (de) | 2000-09-20 |
| DE69033633T2 (de) | 2001-05-03 |
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