JP2000511406A - レプチンアッセイ - Google Patents

レプチンアッセイ

Info

Publication number: JP2000511406A
Authority: JP; Japan
Prior art keywords: leptin; promoter; nucleic acid; response element; cells
Prior art date: 1996-04-22
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

JP09538203A

Other languages

English (en)

Japanese (ja)

Inventor

ローゼンブラム，チヤールズ・アイ

フアン・デル・プルウフ，レオナルドウス

クレシ，サジヤド・エイ

カリ，ドリス・エフ

ヘス，ジヨン・ダブリユ

トタ，マイケル・アール

チエン，フアン

Original Assignee

メルクエンドカンパニーインコーポレーテッド

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-04-22

Filing date

1997-04-18

Publication date

2000-09-05

1996-06-06 Priority claimed from GBGB9611785.8A external-priority patent/GB9611785D0/en

1997-04-18 Application filed by メルクエンドカンパニーインコーポレーテッド filed Critical メルクエンドカンパニーインコーポレーテッド

2000-09-05 Publication of JP2000511406A publication Critical patent/JP2000511406A/ja

Status Pending legal-status Critical Current

Links

229940039781 leptin Drugs 0.000 title claims abstract description 75
102000016267 Leptin Human genes 0.000 title claims abstract description 73
108010092277 Leptin Proteins 0.000 title claims abstract description 73
NRYBAZVQPHGZNS-ZSOCWYAHSA-N leptin Chemical compound O=C([C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CC(C)C)CCSC)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CS)C(O)=O NRYBAZVQPHGZNS-ZSOCWYAHSA-N 0.000 title claims abstract description 72
238000003556 assay Methods 0.000 title abstract description 19
108091027981 Response element Proteins 0.000 claims abstract description 33
108700008625 Reporter Genes Proteins 0.000 claims abstract description 30
108700039691 Genetic Promoter Regions Proteins 0.000 claims abstract description 23
108010019813 leptin receptors Proteins 0.000 claims description 52
102000005861 leptin receptors Human genes 0.000 claims description 51
108020004707 nucleic acids Proteins 0.000 claims description 20
102000039446 nucleic acids Human genes 0.000 claims description 20
150000007523 nucleic acids Chemical class 0.000 claims description 20
238000013518 transcription Methods 0.000 claims description 18
230000035897 transcription Effects 0.000 claims description 18
238000000034 method Methods 0.000 claims description 17
239000013598 vector Substances 0.000 claims description 17
230000027455 binding Effects 0.000 claims description 15
150000001875 compounds Chemical class 0.000 claims description 13
230000000694 effects Effects 0.000 claims description 13
108090000623 proteins and genes Proteins 0.000 claims description 10
108090000890 Interferon regulatory factor 1 Proteins 0.000 claims description 8
108010074328 Interferon-gamma Proteins 0.000 claims description 8
102000004289 Interferon regulatory factor 1 Human genes 0.000 claims description 7
102000008070 Interferon-gamma Human genes 0.000 claims description 7
108060001084 Luciferase Proteins 0.000 claims description 7
239000005089 Luciferase Substances 0.000 claims description 7
229940044627 gamma-interferon Drugs 0.000 claims description 7
230000003213 activating effect Effects 0.000 claims description 6
241000283984 Rodentia Species 0.000 claims description 2
235000020004 porter Nutrition 0.000 claims 1
230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 abstract 1
210000004027 cell Anatomy 0.000 description 55
241000700159 Rattus Species 0.000 description 9
239000012634 fragment Substances 0.000 description 9
108020003175 receptors Proteins 0.000 description 9
108020004414 DNA Proteins 0.000 description 8
238000013519 translation Methods 0.000 description 8
239000002299 complementary DNA Substances 0.000 description 7
102000005962 receptors Human genes 0.000 description 7
108091028043 Nucleic acid sequence Proteins 0.000 description 6
102000005936 beta-Galactosidase Human genes 0.000 description 5
108010005774 beta-Galactosidase Proteins 0.000 description 5
239000003446 ligand Substances 0.000 description 5
239000002773 nucleotide Substances 0.000 description 5
125000003729 nucleotide group Chemical group 0.000 description 5
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108091092195 Intron Proteins 0.000 description 4
241000699666 Mus <mouse, genus> Species 0.000 description 4
108091034117 Oligonucleotide Proteins 0.000 description 4
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108091026890 Coding region Proteins 0.000 description 3
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239000000556 agonist Substances 0.000 description 3
239000005557 antagonist Substances 0.000 description 3
239000001963 growth medium Substances 0.000 description 3
230000002267 hypothalamic effect Effects 0.000 description 3
238000012163 sequencing technique Methods 0.000 description 3
108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 2
239000006144 Dulbecco’s modiﬁed Eagle's medium Substances 0.000 description 2
102000004190 Enzymes Human genes 0.000 description 2
108090000790 Enzymes Proteins 0.000 description 2
108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
101001063991 Homo sapiens Leptin Proteins 0.000 description 2
101001129927 Homo sapiens Leptin receptor Proteins 0.000 description 2
208000008589 Obesity Diseases 0.000 description 2
241000700584 Simplexvirus Species 0.000 description 2
108020004440 Thymidine kinase Proteins 0.000 description 2
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
230000004913 activation Effects 0.000 description 2
239000013592 cell lysate Substances 0.000 description 2
230000036755 cellular response Effects 0.000 description 2
239000003153 chemical reaction reagent Substances 0.000 description 2
238000001514 detection method Methods 0.000 description 2
206010012601 diabetes mellitus Diseases 0.000 description 2
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
239000013604 expression vector Substances 0.000 description 2
210000002950 fibroblast Anatomy 0.000 description 2
239000005090 green fluorescent protein Substances 0.000 description 2
102000049953 human LEP Human genes 0.000 description 2
102000007318 human leptin receptor Human genes 0.000 description 2
210000003016 hypothalamus Anatomy 0.000 description 2
230000003834 intracellular effect Effects 0.000 description 2
239000002502 liposome Substances 0.000 description 2
238000003670 luciferase enzyme activity assay Methods 0.000 description 2
239000012528 membrane Substances 0.000 description 2
239000000203 mixture Substances 0.000 description 2
238000001823 molecular biology technique Methods 0.000 description 2
239000002953 phosphate buffered saline Substances 0.000 description 2
108090000765 processed proteins & peptides Proteins 0.000 description 2
102000004169 proteins and genes Human genes 0.000 description 2
238000012216 screening Methods 0.000 description 2
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
238000011144 upstream manufacturing Methods 0.000 description 2
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
101100366892 Anopheles gambiae Stat gene Proteins 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
206010006895 Cachexia Diseases 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
241000699802 Cricetulus griseus Species 0.000 description 1
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
101100366894 Drosophila melanogaster Stat92E gene Proteins 0.000 description 1
241000588724 Escherichia coli Species 0.000 description 1
239000004606 Fillers/Extenders Substances 0.000 description 1
108090000331 Firefly luciferases Proteins 0.000 description 1
241000282412 Homo Species 0.000 description 1
102100037850 Interferon gamma Human genes 0.000 description 1
108010047761 Interferon-alpha Proteins 0.000 description 1
102000006992 Interferon-alpha Human genes 0.000 description 1
241000254158 Lampyridae Species 0.000 description 1
FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
206010029260 Neuroblastoma Diseases 0.000 description 1
229930182555 Penicillin Natural products 0.000 description 1
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
101001129923 Rattus norvegicus Leptin receptor Proteins 0.000 description 1
235000002597 Solanum melongena Nutrition 0.000 description 1
244000061458 Solanum melongena Species 0.000 description 1
102000006601 Thymidine Kinase Human genes 0.000 description 1
230000003321 amplification Effects 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
208000022531 anorexia Diseases 0.000 description 1
230000004071 biological effect Effects 0.000 description 1
230000008512 biological response Effects 0.000 description 1
239000000872 buffer Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
239000011575 calcium Substances 0.000 description 1
244000309466 calf Species 0.000 description 1
238000004113 cell culture Methods 0.000 description 1
239000006143 cell culture medium Substances 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
208000012696 congenital leptin deficiency Diseases 0.000 description 1
238000010276 construction Methods 0.000 description 1
239000013601 cosmid vector Substances 0.000 description 1
230000009089 cytolysis Effects 0.000 description 1
206010061428 decreased appetite Diseases 0.000 description 1
238000013461 design Methods 0.000 description 1
230000029087 digestion Effects 0.000 description 1
231100000673 dose–response relationship Toxicity 0.000 description 1
239000003623 enhancer Substances 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
230000035558 fertility Effects 0.000 description 1
230000001605 fetal effect Effects 0.000 description 1
230000037406 food intake Effects 0.000 description 1
235000012631 food intake Nutrition 0.000 description 1
238000002825 functional assay Methods 0.000 description 1
230000006698 induction Effects 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
210000003734 kidney Anatomy 0.000 description 1
229910052749 magnesium Inorganic materials 0.000 description 1
239000011777 magnesium Substances 0.000 description 1
210000004962 mammalian cell Anatomy 0.000 description 1
238000005259 measurement Methods 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
108020004999 messenger RNA Proteins 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
208000001022 morbid obesity Diseases 0.000 description 1
231100000350 mutagenesis Toxicity 0.000 description 1
230000035772 mutation Effects 0.000 description 1
OHDXDNUPVVYWOV-UHFFFAOYSA-N n-methyl-1-(2-naphthalen-1-ylsulfanylphenyl)methanamine Chemical compound CNCC1=CC=CC=C1SC1=CC=CC2=CC=CC=C12 OHDXDNUPVVYWOV-UHFFFAOYSA-N 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
235000020824 obesity Nutrition 0.000 description 1
210000001672 ovary Anatomy 0.000 description 1
229940049954 penicillin Drugs 0.000 description 1
239000013600 plasmid vector Substances 0.000 description 1
239000000376 reactant Substances 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
230000001105 regulatory effect Effects 0.000 description 1
230000004044 response Effects 0.000 description 1
230000004043 responsiveness Effects 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
239000000243 solution Substances 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
239000000126 substance Substances 0.000 description 1
230000001629 suppression Effects 0.000 description 1
208000024891 symptom Diseases 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
208000001072 type 2 diabetes mellitus Diseases 0.000 description 1
239000013603 viral vector Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Wood Science & Technology (AREA)
Toxicology (AREA)
Immunology (AREA)
Gastroenterology & Hepatology (AREA)
Engineering & Computer Science (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Cell Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Peptides Or Proteins (AREA)

JP09538203A 1996-04-22 1997-04-18 レプチンアッセイ Pending JP2000511406A (ja)

Applications Claiming Priority (7)

Application Number	Priority Date	Filing Date	Title
US1605196P	1996-04-22	1996-04-22
GBGB9611785.8A GB9611785D0 (en)	1996-06-06	1996-06-06	Leptin assay
US3100296P	1996-11-15	1996-11-15
US60/016,051		1996-11-15
US60/031,002		1996-11-15
US9611785.8		1996-11-15
PCT/US1997/006505 WO1997040380A1 (fr)	1996-04-22	1997-04-18	Dosage de la leptine

Publications (1)

Publication Number	Publication Date
JP2000511406A true JP2000511406A (ja)	2000-09-05

Family

ID=27268306

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP09538203A Pending JP2000511406A (ja)	1996-04-22	1997-04-18	レプチンアッセイ

Country Status (4)

Country	Link
EP (1)	EP0914611A4 (fr)
JP (1)	JP2000511406A (fr)
CA (1)	CA2252443A1 (fr)
WO (1)	WO1997040380A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6310034B1 (en)	1993-05-21	2001-10-30	Ut-Battelle, Llc	Agouti polypeptide compositions
US6001968A (en) *	1994-08-17	1999-12-14	The Rockefeller University	OB polypeptides, modified forms and compositions
US7084252B1 (en)	1996-01-16	2006-08-01	The Rockefeller University	DB, the receptor for leptin
US7063958B1 (en)	1996-01-16	2006-06-20	The Rockefeller University	Nucleic acids db, the receptor for leptin
US7148004B1 (en)	1997-01-16	2006-12-12	The Rockefeller University	Oligonucleotides of the OB-R isoforms and methods of diagnosing body weight
US7619079B2 (en)	1996-02-14	2009-11-17	The Rockefeller University	Db, the receptor for leptin, nucleic acids encoding the receptor, and uses thereof
PL333187A1 (en) *	1996-11-01	1999-11-22	Smithkline Beecham Plc	Method of detecting compounds which modulate the performance of protein of obesity (ob)
EP1101112B1 (fr) *	1998-07-28	2004-10-06	Vlaams Interuniversitair Instituut voor Biotechnologie vzw.	Induction de genes mediee par la leptine
IL132313A0 (en) *	1999-10-11	2001-03-19	Yeda Res & Dev	Leptin assay

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5401629A (en) *	1990-08-07	1995-03-28	The Salk Institute Biotechnology/Industrial Associates, Inc.	Assay methods and compositions useful for measuring the transduction of an intracellular signal
US5643748A (en) *	1994-09-14	1997-07-01	Progenitor, Inc.	HU-B1.219, a novel human hematopoietin receptor
PL323638A1 (en) *	1995-05-30	1998-04-14	Smithkline Beecham Plc	Method of detecting obesity protein modulating compounds

1997
- 1997-04-18 EP EP97924507A patent/EP0914611A4/fr not_active Withdrawn
- 1997-04-18 WO PCT/US1997/006505 patent/WO1997040380A1/fr not_active Ceased
- 1997-04-18 CA CA 2252443 patent/CA2252443A1/fr not_active Abandoned
- 1997-04-18 JP JP09538203A patent/JP2000511406A/ja active Pending

Also Published As

Publication number	Publication date
EP0914611A1 (fr)	1999-05-12
EP0914611A4 (fr)	2002-10-16
WO1997040380A1 (fr)	1997-10-30
CA2252443A1 (fr)	1997-10-30

Publication	Publication Date	Title
Liu et al.	1995	Differential expression of human Fas mRNA species upon peripheral blood mononuclear cell activation
Li et al.	1998	Cloning and characterization of a novel human hepatocyte transcription factor, hB1F, which binds and activates enhancer II of hepatitis B virus
Cordle et al.	1991	Pancreatic β-cell-type-specific transcription of the insulin gene is mediated by basic helix-loop-helix DNA-binding proteins
US6007998A (en)	1999-12-28	Leptin assay
KR20040010401A (ko)	2004-01-31	안드로겐 수용체 복합체-결합 단백질
JP2000511406A (ja)	2000-09-05	レプチンアッセイ
Autelitano	1998	Cardiac expression of genes encoding putative adrenomedullin/calcitonin gene-related peptide receptors
CA2554380C (fr)	2015-05-19	Gene mecp2e1
Atanasoski et al.	1995	Isolation of the human genomic brain-2/N-Oct 3 gene (POUF3) and assignment to chromosome 6q16
CZ20021853A3 (cs)	2002-11-13	Způsob testování účinku látky, polynukleotid, DNA, protilátka, souprava pro testování, pouľití polynukleotidu, pouľití polypeptidu, pouľití protilátky, RNA a terapeutické činidlo
CN1192248A (zh)	1998-09-02	检测调节肥胖症蛋白作用的化合物的方法
JP2004187620A (ja)	2004-07-08	腎疾患の疾患マーカーおよびその利用
Matre et al.	2003	The human neuroendocrine thyrotropin-releasing hormone receptor promoter is activated by the haematopoietic transcription factor c-Myb
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