JP2016525344A5 - - Google Patents

Download PDF

Info

Publication number
JP2016525344A5
JP2016525344A5 JP2016519541A JP2016519541A JP2016525344A5 JP 2016525344 A5 JP2016525344 A5 JP 2016525344A5 JP 2016519541 A JP2016519541 A JP 2016519541A JP 2016519541 A JP2016519541 A JP 2016519541A JP 2016525344 A5 JP2016525344 A5 JP 2016525344A5
Authority
JP
Japan
Prior art keywords
target
nucleotide sequence
probe
sample
identified nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2016519541A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016525344A (ja
JP6600302B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2014/040731 external-priority patent/WO2014200767A1/en
Publication of JP2016525344A publication Critical patent/JP2016525344A/ja
Publication of JP2016525344A5 publication Critical patent/JP2016525344A5/ja
Application granted granted Critical
Publication of JP6600302B2 publication Critical patent/JP6600302B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2016519541A 2013-06-12 2014-06-03 標的分子のマルチプレックス検出のための方法、キット、およびシステム、ならびにそれらの使用 Active JP6600302B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361834111P 2013-06-12 2013-06-12
US61/834,111 2013-06-12
US201361912054P 2013-12-05 2013-12-05
US61/912,054 2013-12-05
US201461972940P 2014-03-31 2014-03-31
US61/972,940 2014-03-31
PCT/US2014/040731 WO2014200767A1 (en) 2013-06-12 2014-06-03 Methods, kits, and systems for multiplexed detection of target molecules and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019100854A Division JP6785338B2 (ja) 2013-06-12 2019-05-30 標的分子のマルチプレックス検出のための方法、キット、およびシステム、ならびにそれらの使用

Publications (3)

Publication Number Publication Date
JP2016525344A JP2016525344A (ja) 2016-08-25
JP2016525344A5 true JP2016525344A5 (2) 2017-07-13
JP6600302B2 JP6600302B2 (ja) 2019-10-30

Family

ID=52022666

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2016519541A Active JP6600302B2 (ja) 2013-06-12 2014-06-03 標的分子のマルチプレックス検出のための方法、キット、およびシステム、ならびにそれらの使用
JP2019100854A Active JP6785338B2 (ja) 2013-06-12 2019-05-30 標的分子のマルチプレックス検出のための方法、キット、およびシステム、ならびにそれらの使用

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2019100854A Active JP6785338B2 (ja) 2013-06-12 2019-05-30 標的分子のマルチプレックス検出のための方法、キット、およびシステム、ならびにそれらの使用

Country Status (7)

Country Link
US (2) US10266874B2 (2)
EP (2) EP3587585B1 (2)
JP (2) JP6600302B2 (2)
AU (3) AU2014278537B2 (2)
CA (1) CA2915033C (2)
ES (2) ES2754177T3 (2)
WO (1) WO2014200767A1 (2)

Families Citing this family (146)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
WO2011127099A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Spatially encoded biological assays
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4592400A3 (en) 2012-10-17 2025-10-29 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20200140929A (ko) 2013-02-08 2020-12-16 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
ES2966631T3 (es) 2013-06-19 2024-04-23 Univ California Estructuras químicas para el suministro localizado de agentes terapéuticos
CA2916662C (en) 2013-06-25 2022-03-08 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
KR102758333B1 (ko) 2013-08-28 2025-01-23 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
CN106659796B (zh) 2014-03-14 2021-04-30 加利福尼亚大学董事会 Tco缀合物和治疗剂递送方法
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
WO2015200893A2 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
JP6769969B2 (ja) 2015-01-12 2020-10-14 10エックス ジェノミクス, インコーポレイテッド 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP3901282B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
SG10202107053QA (en) * 2015-07-17 2021-08-30 Nanostring Technologies Inc Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
WO2017015097A1 (en) * 2015-07-17 2017-01-26 Nanostring Technologies, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
WO2017044983A1 (en) 2015-09-10 2017-03-16 Shasqi, Inc. Bioorthogonal compositions
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ, インコーポレイテッド ライブラリー正規化のための方法および組成物
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
JP6954899B2 (ja) 2015-12-04 2021-10-27 10エックス ゲノミクス,インコーポレイテッド 核酸の解析のための方法及び組成物
US20200408687A1 (en) * 2016-03-25 2020-12-31 The General Hospital Corporation Polarization-Based Fluorescent Nucleic Acid Detection
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CA3029178C (en) * 2016-06-30 2022-07-19 Biois Co.,Ltd Double-stranded nucleic acid signal probe and method for detecting target molecule using same
WO2018017469A1 (en) * 2016-07-18 2018-01-25 Yale University Multiplex detection of intracellular or surface molecular targets in single cells
AU2017302300B2 (en) * 2016-07-27 2023-08-17 The Board Of Trustees Of The Leland Stanford Junior University Highly-multiplexed fluorescent imaging
CN109791157B (zh) * 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
IL266197B2 (en) 2016-10-24 2024-03-01 Geneinfosec Inc Hiding information contained within nucleic acids
EP3554556B1 (en) * 2016-12-19 2022-03-09 Ventana Medical Systems, Inc. Peptide nucleic acid conjugates
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
CN110520541B (zh) 2017-02-13 2024-03-26 耶鲁大学 高通量单细胞多组学
AU2018250312C1 (en) 2017-04-07 2024-08-22 Tambo, Inc. Bioorthogonal compositions
JP6990456B2 (ja) * 2017-05-02 2022-01-12 国立大学法人 東京大学 細胞またはその由来物の動的変化のモニタリング方法およびそれを用いた細胞分類方法
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN110719959B (zh) 2017-06-05 2021-08-06 贝克顿迪金森公司 针对单细胞的样品索引
US11084037B2 (en) * 2017-09-25 2021-08-10 Plexium, Inc. Oligonucleotide encoded chemical libraries
US20200256862A1 (en) * 2017-10-23 2020-08-13 Massachusetts Institute Of Technology Functionalized solid support
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3724658A1 (en) * 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
JP7727385B2 (ja) 2017-12-18 2025-08-21 ヴェンタナ メディカル システムズ, インク. ペプチド核酸コンジュゲート
JP7158642B2 (ja) * 2018-01-16 2022-10-24 国立大学法人山梨大学 質量分析装置及び質量分析システム
KR101911859B1 (ko) * 2018-02-02 2018-12-28 주식회사 바이오이즈 이중가닥 핵산 신호 프로브 및 이를 이용한 표적분자의 검출방법
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
US20190249248A1 (en) 2018-02-12 2019-08-15 Nanostring Technologies, Inc. Biomolecular probes and methods of detecting gene and protein expression
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
JP7358388B2 (ja) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー 反対側の転写物末端における分子バーコーディング
CN110456034B (zh) * 2018-05-07 2022-07-22 上海市第十人民医院 一种循环肿瘤细胞的检测方法
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US12559787B2 (en) * 2018-05-21 2026-02-24 The Regents Of The University Of California Single cell mapping and transcriptome analysis
US20200020419A1 (en) * 2018-07-16 2020-01-16 Flagship Pioneering Innovations Vi, Llc. Methods of analyzing cells
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
CN113454238B (zh) 2019-01-25 2025-02-11 哈佛大学校长及研究员协会 用于合成核酸的组合物和方法
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
EP3938538A1 (en) 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
EP3887542A1 (en) 2019-03-22 2021-10-06 10X Genomics, Inc. Three-dimensional spatial analysis
JP7183899B2 (ja) * 2019-03-25 2022-12-06 株式会社Jvcケンウッド 分析装置及び分析方法
CA3134973A1 (en) * 2019-03-27 2020-10-01 Fluidigm Canada Inc. Lyophilized antibody panel
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
KR102406463B1 (ko) * 2019-09-03 2022-06-10 재단법인 오송첨단의료산업진흥재단 단백질 생산성 향상을 위한 개별 바코딩 시스템 도입한 시그널 펩타이드 초고속 선별방법
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
ES3024383T3 (en) 2019-11-13 2025-06-04 10X Genomics Inc Generating capture probes for spatial analysis
EP4073246A4 (en) 2019-12-12 2023-12-06 President And Fellows Of Harvard College COMPOSITIONS AND METHODS FOR LIGHT-DIRECTED BIOMOLECULAR BARCODING
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US12577702B2 (en) 2020-01-28 2026-03-17 Plexium, Inc. Oligonucleotide encoded chemical libraries, related systems, devices, and methods for detecting, analyzing, quantifying, and testing biologics/genetics
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
EP4105340A4 (en) * 2020-02-14 2023-08-09 Sony Group Corporation ANALYTICAL METHOD, ANALYTICAL SYSTEM AND INTERFACE FOR ANALYSIS
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US20240018607A1 (en) * 2020-03-31 2024-01-18 Logicink Corporation Molecular beacons
CN115997030A (zh) 2020-04-30 2023-04-21 德迈逊科技有限公司 用于大分子操作的装置和方法
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
WO2021243285A1 (en) * 2020-05-29 2021-12-02 Seer, Inc. Systems and methods for rapid identification of proteins
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN116056765A (zh) 2020-08-07 2023-05-02 坦伯公司 反式环辛烯生物正交剂及在癌症和免疫疗法中的用途
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
WO2022118862A1 (ja) * 2020-12-01 2022-06-09 国立大学法人九州大学 生体分子構造検出用プローブ、生体分子構造検出用キット、及び生体分子構造の検出方法
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022147296A1 (en) 2020-12-30 2022-07-07 10X Genomics, Inc. Cleavage of capture probes for spatial analysis
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
EP4341847A1 (en) * 2021-05-19 2024-03-27 Leica Microsystems CMS GmbH Marker, method and device for analyzing a biological sample
US20240255425A1 (en) * 2021-05-19 2024-08-01 Leica Microsystems Cms Gmbh Marker, method and device for analyzing a biological sample
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023034789A1 (en) * 2021-08-31 2023-03-09 Becton, Dickinson And Company Multiplexed proteomics analysis using oligonucleotide-conjugated antibodies
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
US20250144110A1 (en) * 2022-01-28 2025-05-08 Washington University Compositions of autophagy modulating agents and uses thereof
WO2023250077A1 (en) 2022-06-22 2023-12-28 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4343326B1 (en) * 2022-09-20 2025-03-12 Miltenyi Biotec B.V. & Co. KG Biological conjugates having an photogenerated acidic or basic releasable detection moiety on top of biological tissues
US20260098358A1 (en) * 2022-09-22 2026-04-09 Astrin Biosciences, Inc. Tumor cell profiling-based therapeutic targeting of cancer
WO2024073524A2 (en) * 2022-09-28 2024-04-04 Massachusetts Institute Of Technology High throughput stochastic bio-molecular sensor
EP4624590A3 (en) 2022-12-30 2025-12-24 10X Genomics, Inc. Methods, compositions, and kits for multiple barcoding and/or high-density spatial barcoding
KR102869006B1 (ko) * 2023-03-14 2025-10-14 재단법인 아산사회복지재단 다중면역형광염색을 이용한 세포 시료에서 표적 단백질을 검출하는 방법
WO2025049491A1 (en) * 2023-08-29 2025-03-06 Genopheno, Llc. Novel sampling and genetic comparison and analysis of cannabis components for commercial differentiation
WO2025188805A1 (en) * 2024-03-04 2025-09-12 University Of Denver Blood based rapid diagnostic system for brain hemorrhage detection and associated methods
US20250329467A1 (en) * 2024-04-23 2025-10-23 Delfi Diagnostics, Inc. Workflows for discovery and deployment of diagnostic assays combining proteomic and genomic information
CN120254253A (zh) * 2025-03-28 2025-07-04 无锡市人民医院 一种单细胞检测方法

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US5093268A (en) 1988-04-28 1992-03-03 Igen, Inc. Apparatus for conducting a plurality of simultaneous measurements of electrochemiluminescent phenomena
DE3920358A1 (de) 1989-06-22 1991-01-17 Behringwerke Ag Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung
ES2259800T3 (es) 1990-06-11 2006-10-16 Gilead Sciences, Inc. Procedimientos de uso de ligandos de acido nucleico.
ATE207080T1 (de) 1991-11-25 2001-11-15 Enzon Inc Multivalente antigen-bindende proteine
US6136540A (en) 1994-10-03 2000-10-24 Ikonisys Inc. Automated fluorescence in situ hybridization detection of genetic abnormalities
US5430175A (en) 1993-11-30 1995-07-04 Cornell Research Foundation, Inc. Caged carboxyl compounds and use thereof
US5888829A (en) 1994-11-08 1999-03-30 Molecular Probes, Inc. Photolabile caged ionophores and method of using in a membrane separation process
US5635608A (en) 1994-11-08 1997-06-03 Molecular Probes, Inc. α-carboxy caged compounds
JPH11502617A (ja) 1995-03-10 1999-03-02 メソ スケール テクノロジーズ,エルエルシー 多重アレイの多重特異的な電気化学発光試験
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US5872243A (en) 1995-06-30 1999-02-16 Molecular Probes, Inc. Caged nucleotides
US6043065A (en) 1997-12-23 2000-03-28 University Of Maryland Biotechnology Institute Photosensitive organic compounds that release 2,5,-di(tert-butyl)hydroquinone upon illumination
US6472541B2 (en) 1998-11-20 2002-10-29 The Regents Of The University Of California Protecting groups with increased photosensitivities
US20070009931A1 (en) * 2000-08-04 2007-01-11 Kirsch Wolff M Negative correlation between IRP-2 and Transferrin receptor expression as a diagnostic of Alzheimer's disease
WO2002079514A1 (en) 2001-01-10 2002-10-10 The Trustees Of Boston College Dna-bridged carbon nanotube arrays
EP1384022A4 (en) 2001-04-06 2004-08-04 California Inst Of Techn NUCLEIC ACID AMPLIFICATION USING MICROFLUID DEVICES
WO2003048295A1 (en) 2001-11-30 2003-06-12 Fluidigm Corporation Microfluidic device and methods of using same
US20030194711A1 (en) 2002-04-10 2003-10-16 Matthew Zapala System and method for analyzing gene expression data
AU2003286739A1 (en) 2002-10-28 2004-05-25 University Of Washington Wavelength tunable surface plasmon resonance sensor
EP1660858A4 (en) 2003-07-21 2007-10-24 Amplified Proteomics Inc MULTIPLEX analyte
US20080318340A1 (en) 2004-11-01 2008-12-25 Tokyo Metropolitan Organization For Medical Resear Method of Detecting Target Substances
WO2007076128A2 (en) 2005-12-23 2007-07-05 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
JP5700911B2 (ja) 2005-12-23 2015-04-15 ナノストリング テクノロジーズ,インコーポレーテッド 配向され、固定化された巨大分子を含む組成物とその製造法
CA2653095C (en) 2006-05-22 2013-07-16 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
CA2687292C (en) 2007-04-10 2017-07-04 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
US8865401B2 (en) * 2007-12-14 2014-10-21 The Johns Hopkins University Purification and concentration of proteins and DNA from a complex sample using isotachophoresis and a device to perform the purification
US7923562B2 (en) * 2008-06-16 2011-04-12 The Board Of Trustees Of The Leland Stanford Junior University Photocleavable linker methods and compositions
US9000142B2 (en) 2008-07-07 2015-04-07 Syntrix Biosystems, Inc. Photocleavable sense-antisense complex
US8519115B2 (en) 2008-08-14 2013-08-27 Nanostring Technologies, Inc. Stable nanoreporters
CA2749601C (en) 2009-01-14 2020-07-21 Stephen M. Hewitt Ratio based biomarkers and methods of use thereof
WO2011047087A2 (en) 2009-10-13 2011-04-21 Nanostring Technologies, Inc. Protein detection via nanoreporters
CA2789038A1 (en) * 2010-02-08 2011-08-11 Isis Pharmaceuticals, Inc. Selective reduction of allelic variants
EP2516681B1 (en) 2010-02-11 2017-10-18 Nanostring Technologies, Inc Compositions and methods for the detection of preferably small rnas by bridge hybridisation and ligation
US20110229888A1 (en) 2010-03-16 2011-09-22 Hengen Paul N Compositions and Methods for the Detection of Genomic Features
US20130059741A1 (en) * 2010-05-13 2013-03-07 Illumina, Inc. Binding assays for markers
US9110079B2 (en) * 2010-09-29 2015-08-18 Biomerieux Method and kit for establishing an in vitro prognosis on a patient exhibiting SIRS
US9758834B2 (en) 2011-03-28 2017-09-12 Nanostring Technologies, Inc. Compositions and methods for diagnosing cancer
JP2013027387A (ja) 2011-06-21 2013-02-07 Tohoku Univ 膵臓癌バイオマーカー
CA2839705A1 (en) 2011-06-24 2012-12-27 Nanostring Technologies, Inc. Multivariate diagnostic assays and methods for using same
US20130095483A1 (en) * 2011-10-17 2013-04-18 Nuclea Biotechnologies, Inc. Predictive biomarkers for breast cancer
WO2013078332A1 (en) 2011-11-23 2013-05-30 The General Hospital Corporation Analyte detection using magnetic hall effect
US20140037620A1 (en) 2012-06-29 2014-02-06 British Columbia Cancer Agency Branch Methods of Treating Breast Cancer with Gemcitabine Therapy
WO2014144325A1 (en) * 2013-03-15 2014-09-18 President And Fellows Of Harvard College Methods and compositions for improving detection and/or capture of a target entity

Similar Documents

Publication Publication Date Title
JP2016525344A5 (2)
AU2014373622B2 (en) Nucleic acid detection method and kit
CN104812915A (zh) 基于pcr用于平行检测生物材料的测定
EP3277834B1 (en) Methods of capturing sperm nucleic acids
CN104024426B (zh) Dna二代测序技术中待测样品的追踪方法及检测试剂盒
JP2022504078A5 (2)
WO2017181339A1 (zh) 蛋白配体和基因同时检测方法及试剂盒
CN117625764A (zh) 准确地平行检测和定量核酸的方法
CN109706269B (zh) 能检测多种禽呼吸道病原的多重连接探针扩增鉴别试剂盒
CN104278079A (zh) 用核酸层析技术检测核酸的试纸条和方法
CN113186253B (zh) 一种用于检测路易体病标志物的Cas12a-DNAzyme传感器及其制备方法
CN107312873A (zh) 一种快速区分5种小鼠呼吸道病原的多重液相基因芯片检测引物、试剂盒及方法
JP4672234B2 (ja) 細胞または組織試料におけるポリヌクレオチド量の変動の定量のための方法および試験キット
CN101215600B (zh) 核酸目的序列的检测探针及检测方法
Rodrigo et al. Electrochemical microarray for identification pathogens: a review
CN104498591B (zh) 基于液相芯片定量检测Alu基因甲基化水平的方法
AU2002226448A1 (en) A method and test kit for quantitative determination of variations in polynucleotide amounts in cell or tissue samples
CN105349700A (zh) 一种快速鉴别小鼠细小病毒mvm株与mpv株的hrm检测方法及引物
JP2005528909A (ja) コンビナトリアルオリゴヌクレオチドpcrの改善方法
CN106367530B (zh) 一种快速鉴别四种大鼠细小病毒株的引物组及检测方法
CN107034274B (zh) 一种水貂自咬行为诊断基因及其诊断方法
JP6468555B2 (ja) 検出キット,及び検出方法
KR101493903B1 (ko) 샬롯노란줄무늬 바이러스 검출용 프라이머 세트 및 이의 용도
KR101356396B1 (ko) Pcr 수행 없는 소위성 주형 반복 유전자 타이핑법
JP2010517556A5 (2)