JP6869379B2 - 血管モデル - Google Patents

血管モデル Download PDF

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Publication number
JP6869379B2
JP6869379B2 JP2019565825A JP2019565825A JP6869379B2 JP 6869379 B2 JP6869379 B2 JP 6869379B2 JP 2019565825 A JP2019565825 A JP 2019565825A JP 2019565825 A JP2019565825 A JP 2019565825A JP 6869379 B2 JP6869379 B2 JP 6869379B2
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Japan
Prior art keywords
porous membrane
blood vessel
cell layer
hole
vessel model
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JP2019565825A
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Japanese (ja)
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JP2020521974A (ja
Inventor
晃寿 伊藤
晃寿 伊藤
千早 柿沼
千早 柿沼
大地 引本
大地 引本
伸治 美馬
伸治 美馬
貴史 末広
貴史 末広
ネヴィル、クレイグ、エム．
サンドバック、キャスリン、エー．
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Fujifilm Corp
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Fujifilm Corp
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Priority claimed from US15/618,151 external-priority patent/US20180356399A1/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0691—Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/08—Investigating permeability, pore-volume, or surface area of porous materials
    • G01N15/0806—Details, e.g. sample holders, mounting samples for testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5064—Endothelial cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1347—Smooth muscle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1352—Mesenchymal stem cells
    • C12N2502/1358—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/08—Investigating permeability, pore-volume, or surface area of porous materials
    • G01N2015/086—Investigating permeability, pore-volume, or surface area of porous materials of films, membranes or pellicules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Vascular Medicine (AREA)
  • Fluid Mechanics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Materials For Medical Uses (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2019565825A 2017-06-09 2018-06-07 血管モデル Active JP6869379B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US15/618,151 US20180356399A1 (en) 2017-06-09 2017-06-09 Blood vessel model
US15/618,151 2017-06-09
US201862640589P 2018-03-09 2018-03-09
US62/640,589 2018-03-09
PCT/US2018/036364 WO2018226902A2 (fr) 2017-06-09 2018-06-07 Modèle de vaisseau sanguin

Publications (2)

Publication Number Publication Date
JP2020521974A JP2020521974A (ja) 2020-07-27
JP6869379B2 true JP6869379B2 (ja) 2021-05-12

Family

ID=64566007

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2019565825A Active JP6869379B2 (ja) 2017-06-09 2018-06-07 血管モデル

Country Status (6)

Country Link
EP (1) EP3635395A4 (fr)
JP (1) JP6869379B2 (fr)
KR (1) KR102345370B1 (fr)
CN (1) CN111263697B (fr)
CA (1) CA3066616C (fr)
WO (1) WO2018226902A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180356398A1 (en) * 2017-06-09 2018-12-13 Fujifilm Corporation Living tissue model device, vascular wall model, vascular wall model device and method of evaluating test substance
US20180356399A1 (en) 2017-06-09 2018-12-13 Fujifilm Corporation Blood vessel model
EP3936603A4 (fr) * 2019-03-28 2022-05-18 FUJIFILM Corporation Dispositif de mesure, programme de fonctionnement pour dispositif de mesure et procédé de mesure
JP2021024089A (ja) * 2019-07-31 2021-02-22 株式会社リコー 立体造形モデル及びその製造方法、並びにハイドロゲル造形物用コーティング剤
EP4174161A4 (fr) 2020-06-25 2025-05-07 Toyo Seikan Group Holdings, Ltd. Dispositif de formation de tissu biologique et procédé de formation de tissu biologique
JP7842545B2 (ja) * 2021-09-27 2026-04-08 東洋製罐グループホールディングス株式会社 マイクロ流体デバイス、及びマイクロ流体デバイスの使用方法
CN114149923B (zh) * 2021-11-19 2024-03-08 大连理工大学 内皮细胞-平滑肌细胞共培养的单流道微芯片模型的构建方法
EP4289934B1 (fr) * 2022-06-09 2025-12-31 Finnadvance Oy Structure de membrane de culture cellulaire, ses procédés de production, plaque de culture cellulaire, appareil de culture cellulaire comprenant la structure de membrane de culture cellulaire et procédés de culture cellulaire à l'aide de l'appareil de culture cellulaire

Family Cites Families (18)

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Publication number Priority date Publication date Assignee Title
JPH0696023B2 (ja) * 1986-11-10 1994-11-30 宇部日東化成株式会社 人工血管およびその製造方法
US7790443B2 (en) * 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
WO2005034624A2 (fr) * 2003-05-21 2005-04-21 The General Hospital Corporation Compositions microfabriquees et procedes de genie tissulaire permettant d'obtenir des tissus contenant des types cellulaires multiples
JP2005298554A (ja) * 2004-04-07 2005-10-27 Sumitomo Electric Ind Ltd 膜厚方向に弾性回復性を有する延伸ポリテトラフルオロエチレン多孔質膜、その製造方法、及び該多孔質膜の使用
JP4904025B2 (ja) * 2004-08-06 2012-03-28 富士フイルム株式会社 フィルムの製造方法
US20060266463A1 (en) * 2005-05-27 2006-11-30 Fuji Photo Film Co., Ltd. Honeycomb composite film, and method for producing the same
US20070243574A1 (en) * 2006-01-27 2007-10-18 University Of Arizona Vascular mimic for drug and device evaluation
EP1999200B1 (fr) * 2006-03-28 2013-07-03 FUJIFILM Corporation Procédé de fabrication d'une pellicule poreuse
JP4734157B2 (ja) * 2006-03-30 2011-07-27 富士フイルム株式会社 ハニカム状多孔質フィルム及びハニカム複合膜
US7943683B2 (en) 2006-12-01 2011-05-17 Tepha, Inc. Medical devices containing oriented films of poly-4-hydroxybutyrate and copolymers
EP2248121A2 (fr) * 2008-02-11 2010-11-10 The General Hospital Corporation Système et procédé de modélisation de vaisseau sanguin in vitro
ES2672201T3 (es) 2008-07-16 2018-06-13 Children's Medical Center Corporation Dispositivo de imitación de órganos con microcanales y métodos de uso
JP2010115624A (ja) * 2008-11-14 2010-05-27 Fuji Xerox Co Ltd マイクロ流路デバイス、分離装置、並びに、分離方法
CN101520960B (zh) * 2009-03-31 2010-09-08 四川大学 体外模拟血管微环境的实验装置
JP2017504320A (ja) * 2013-12-20 2017-02-09 プレジデント アンド フェローズ オブ ハーバード カレッジ 低剪断マイクロ流体デバイスならびにその使用および製造の方法
JP6327618B2 (ja) * 2014-01-08 2018-05-23 学校法人関西医科大学 血管系簡易モデル
US10465155B2 (en) 2015-08-10 2019-11-05 Board Of Regents, The University Of Texas System Non-linear flow path devices and methods for cell culture
WO2017062845A1 (fr) * 2015-10-07 2017-04-13 The Regents Of The University Of Michigan Échafaudages de réparation de nerf ayant un volume de microcanaux élevé et leurs procédés de fabrication

Also Published As

Publication number Publication date
CN111263697B (zh) 2022-04-08
WO2018226902A2 (fr) 2018-12-13
KR102345370B1 (ko) 2021-12-31
CA3066616C (fr) 2022-06-21
EP3635395A4 (fr) 2020-07-29
EP3635395A2 (fr) 2020-04-15
CN111263697A (zh) 2020-06-09
CA3066616A1 (fr) 2018-12-13
WO2018226902A3 (fr) 2020-03-26
KR20200005742A (ko) 2020-01-16
JP2020521974A (ja) 2020-07-27

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