JPH01287100A - Production of soluble calcium caseinate - Google Patents

Abstract

PURPOSE:To obtain the subject compound excellent as a food additive, by heat- treating specific casein in an aqueous solution of an alkali, adding an acidic substance, recovering casein precipitates, suspending the casein precipitates in water and adding calcium hydroxide. CONSTITUTION:At least one selected from acid casein, potassium caseinate and sodium caseinate in an amount of preferably 10-30wt.% (based on an alkaline solution) is heat-treated in the alkaline solution, such as sodium hydroxide, preferably at 40-70 deg.C for 6-24hr and an acidic substance, such as hydrochloric or acetic acid, is added to regulate the pH to about 4.6. Casein precipitated thereby is suspended in water and calcium hydroxide is added so as to provide pH 7 and afford the aimed calcium caseinate.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention relates to a method for producing soluble calcium caseinate (calcium-containing casein).

(Prior Art) Calcium caseinate, which is soluble in water in the neutral range, is expected to be applied as a food additive as a source of protein or calcium. Conventional methods for producing calcium caseinate include adding a saturated solution of calcium hydroxide to qF electrofocus precipitated casein (acid casein) obtained by adding acid to skim milk, and adding a calcium chloride solution to a sodium caseinate solution. Methods are known. Another method is known in which rennet casein containing calcium is obtained by adding rennin to skim milk to coagulate casein.

(Conventional technology section N) Calcium caseinate obtained by conventional technology has a problem in that it has poor solubility in water and is therefore difficult to use as a food additive.

The present inventors completed the present invention as a result of intensive research in order to provide calcium caseinate with excellent solubility in water. caseinate,
At least one selected from sodium caseinate
The seeds are heat treated in an alkaline aqueous solution, an acidic substance is added,
This method is characterized by collecting casein precipitate, suspending the zein precipitate in water, and then adding calcium hydroxide, which will be described in detail below.

(Means for solving the problem) In the present invention, first, acid casein, potassium casein-1,
, sodium caseinate is heat-treated in an alkaline aqueous solution and hydrolyzed. There are no particular restrictions on casein precipitation in an alkaline aqueous solution, but a weight ratio of 10 to 30% is preferable so that the increase in viscosity of the aqueous solution accompanying dissolution of casein does not affect the operability of subsequent steps. . As the alkaline aqueous solution, an aqueous solution of an inorganic or organic alkaline substance can be used without any restriction, but considering that calcium caseinate is used as a food, it is necessary to use a solution of sodium hydroxide, hydroxide, etc. It is preferable to use an aqueous solution of potassium or the like. Among the alkaline substances that are not restricted by the Food Sanitation Law, using an aqueous solution of sodium hydroxide can shorten the reaction time required for hydrolysis, and is particularly preferred because excessive hydrolysis in an alkaline aqueous solution can cause bitterness and decomposition odor. occurs, which reduces the yield of casein precipitate.On the other hand, if hydrolysis is insufficient, the resulting calcium caseinate has poor solubility in water.

In the present invention, an element that adjusts the degree of hydrolysis,
For example, the heat treatment temperature, treatment time, and concentration of alkaline substances in the aqueous solution may be appropriately determined in consideration of the use of calcium caseinate, etc., and there are no particular restrictions on the conditions, but according to the studies of the present inventors. It is preferable to carry out heat treatment at 40 to 80°C for 2 to 48 hours in an alkaline aqueous solution with an alkaline substance concentration of 2 to 30% by weight in terms of yield of calcium caseinate, solubility in water, etc. Alkaline water with a substance concentration of 10-15% by weight! It is more preferable to perform heat treatment at 60 to 70° C. in liquid B for 6 to 24 hours from the viewpoint of the above-mentioned bitterness and decomposition odor.

After heat treatment in an alkaline aqueous solution, an acidic substance is added to the aqueous solution to lower the pH and precipitate casein decomposition products. As the acidic substance, organic and inorganic acidic substances can be used without restriction, but considering that calcium caseinate is used as a food, it is preferable to use hydrochloric acid, acetic acid, etc., which are not restricted by the Food Sanitation Act. The pH of the casein may be lowered to such an extent that casein decomposition products precipitate.
DIM is preferably carried out near the isoelectric point of casein (approximately PH4, 6) in terms of yield and the like.

The precipitated casein decomposition product may be recovered by any conventional method such as centrifugation or filtration.

Subsequently, the collected precipitate is suspended in water, and calcium hydroxide is added to increase the pH of the suspension. Calcium hydroxide may be added so that the pH of the solution is around neutral, preferably pH 7.

Regarding the acidic and alkaline substances that are preferably used in the present invention and are not subject to the restrictions of the Food Sanitation Act, please refer to "Analysis Methods of Food Additives in Foods J (Jll Dansha)."

(Effects of the Invention) According to the method of the present invention, calcium caseinate having excellent solubility in water can be obtained.

This calcium caseinate has little milk-derived taste and odor, and can be applied as a food or food additive.

(Examples) The present invention will be explained in more detail with reference to Examples below, but the present invention is not limited to these Examples.

Example 1 While suspending 1 kg of acid casein in water in an IOL,
Add 150g of sodium hydroxide and heat at 70℃ for 24 hours! ! ! : After stirring, the liquid temperature was lowered to room temperature and hydrochloric acid was added to lower the pH to 4.5. Subsequently, the precipitate of the casein decomposition product was separated by P, and the obtained precipitate was dispersed in water, and calcium hydroxide was added to adjust the pH to 7 to obtain a calcium caseinate solution. Furthermore, after passing through the solution, the powder of calcium caseinate 5 is obtained by spray drying.
00g was obtained. 100m of the obtained calcium caseinate
When dissolved in 1 water, more than 25g was dissolved.

The amount of sodium and calcium contained in the protein was measured using an atomic absorption spectrometer and was found to be 0.07%.
It was 50%.

Example 2 1 kg of skim milk powder was suspended in IOL water, the pH was adjusted to 4.5 by adding hydrochloric acid, and the precipitate was separated by centrifugation. The obtained precipitate was added to 15 L of water together with 100 g of potassium hydroxide, and heated and stirred at 80°C for 5 hours. After the reaction was completed, 40 L of water was added, and acetic acid was added to adjust the pH to 4.
It was lowered to 2.

Subsequently, the precipitate of the casein decomposition product is separated in a furnace, the obtained precipitate is dispersed in water, calcium hydroxide is added, and P
H was adjusted to 7 to obtain a calcium caseinate solution. The resulting solution was filtered and spray-dried to obtain 450 g of calcium caseinate powder. When the obtained calcium caseinate was dissolved in 100ml of water, 20g
The above dissolved. In addition, the amounts of thorium and cardim contained in the protein were measured using an atomic absorption spectrometer and were found to be 0.
10% and 2.45%.

Claims (1)

[Claims] 1. After heat-treating at least one selected from acid casein, potassium caseinate, and sodium caseinate in an alkaline aqueous solution, adding an acidic substance, collecting casein precipitate, and suspending the casein precipitate in water. A method for producing solubilized calcium caseinate, which comprises adding calcium hydroxide.