JPH02154644A - Production of calcium caseinate hydrolyzate - Google Patents

Abstract

PURPOSE:To produce a calcium caseinate hydrolyzate, excellent in solubility and having expected use as a food additive, etc., by suspending acid casein in water in the presence of calcium hydroxide and then reacting the resultant suspension with a protease. CONSTITUTION:Acid casein is suspended in water and calcium hydroxide is then added thereto. The resultant suspension is heated at about 80 deg.C for about 15min. The obtained solution is subsequently cooled and a protease having activity within a neutral or weak alkaline region is then added and reacted therewith while being slowly stirred. The obtained calcium caseinate hydrolyzate is subsequently recovered according to a conventional method.

Description

Detailed Description of the Invention (Industrial Application Field) The present invention relates to a method for producing highly soluble caseinate calcium, and by producing a caseinate calcium decomposition product that is highly soluble in water, it can be used as a beverage or food additive. This is an attempt to expand its uses as a product.

(Prior art) Conventionally, as a method for producing calcium caseinate, iso-weighted precipitated casein (acid casein) obtained by adding acid to skim milk is used.
A method of adding a saturated aqueous solution of calcium hydroxide to a sodium caseinate solution, a method of adding a calcium chloride solution to a sodium caseinate solution, etc. Methods of converting net casein are also known.

(Problem to be solved by the invention) j) Calcium caseinate, which is soluble in water in the neutral fl'l range, is
Although it is expected to be used as a source of protein or calcium or as a food additive, conventional calcium caseinate has unsatisfactory solubility and contains sodium salt, which is undesirable as a food additive. there were.

(Means for Solving the Problem) In view of the above-mentioned current situation, as a result of intensive research, the present inventors suspended acid casein in water in the presence of calcium hydroxide, and then
By enzymatically decomposing acid casein by acting on a protease that is active in a neutral or weakly alkaline region,
It was discovered that calcium caseinate with excellent solubility can be obtained, and the present invention was completed.

That is, in the present invention, hydroxide is added to an aqueous solution in which acid casein, calcium caseinate, or a mixture thereof is suspended, and then a protease having ti'i properties is applied to a neutral or weakly alkaline region to remove decomposed products. The present invention provides a method for producing a decomposed product of calcium caseinate, which comprises recovering the decomposed product of calcium caseinate.

The present invention will be explained in detail below.

As the raw material used in the present invention, acid casein 2 calcium caseinate or a mixture thereof is used.

First, the raw materials are dispersed in an aqueous solution, and then calcium hydroxide is added and heated. It is appropriate to add the raw material in an amount of 2 to 10% of the liquid amount by weight. If the raw material concentration is too high, the operability of the equipment will be poor due to its viscosity, and if it is too low, the economic efficiency will be poor, so it is usually 106 to 15?6, preferably 59
6 to 10% is appropriate. The amount of calcium hydroxide added is not limited to samurai, but it is desirable to adjust the amount of calcium hydroxide added at this time so that the pH of the solution after the reaction is neutral. In addition, by adjusting the amount of calcium hydroxide added at this time, the calcium content of the casein calcium decomposition product i'1 can be adjusted to JQ at will. Usually, it is used in an amount of about 0.1% to 20% by weight based on casein as a raw material.

Since the heating also serves as sterilization L', it is appropriate to perform the heating at about 80° C. for about 15 minutes.

A protease active in a neutral or slightly alkaline region is added to the solution prepared as described above, and the reaction is carried out with gentle stirring. What is the degree of reaction? There are no particular limitations on the enzyme concentration, reaction temperature, reaction time, etc., which are the elements discussed in section 1, but according to the studies of the present inventors, the enzyme concentration is about 5 to 5000 mg per 1 kg of reaction solution. The reaction temperature is preferably 25 to 50°C and the reaction time is preferably about 0.5 to 24 hours.

After the reaction is completed, heat at about 80°C for about 15 minutes to inactivate the enzyme. After that, it is treated with activated carbon to remove the bitter components generated in the reaction, and the resulting solution is dried by a commonly used method such as spray drying to obtain a highly soluble caseinate calcium decomposition product. Can be done.

(Effects of the Invention) The calcium casein decomposition product produced by the method of the present invention has a high solubility in water and dissolves in a wide range of 1)H regions, and the calcium content etc. can be adjusted according to the purpose of use. It is expected to be used as a health food additive.

(Examples) Hereinafter, the present invention will be explained in more detail with reference to Examples, but the present invention is not limited to these Examples.

After cooling, the solution was heated at 80° C. for 15 minutes. A casein decomposition product was obtained by drying the obtained solution with a spray dryer. 100 ml ion exchange water and 1
1 g of the obtained decomposed product was added to each of 0.00 ml of 0.2% citric acid aqueous solution, and its solubility was measured using Biulet's reagent. In ion-exchanged water, it was 6.3+n.
g/ml, Q, 5.8 mg/m for 2% citric acid aqueous solution
It was l. In addition, when sodium ions, potassium ions, calcium ions, etc. were determined by aFI using an atomic absorption spectrometer, thorium ions and potassium ions were 0.1%.
Below, calcium ions were contained by 2.0%. The ash content was measured using a dry ashing method and was found to be 3.7%.

Example 1

Claims (1)

[Claims] A casein characterized in that calcium hydroxide is added to an aqueous solution in which acid casein, calcium caseinate, or a mixture thereof is suspended, and then a protease active in a neutral or weakly alkaline region is applied to recover the decomposed product. Method for producing calcium decomposition product.