JPH0142675B2 - - Google Patents

Info

Publication number
JPH0142675B2
JPH0142675B2 JP58232508A JP23250883A JPH0142675B2 JP H0142675 B2 JPH0142675 B2 JP H0142675B2 JP 58232508 A JP58232508 A JP 58232508A JP 23250883 A JP23250883 A JP 23250883A JP H0142675 B2 JPH0142675 B2 JP H0142675B2
Authority
JP
Japan
Prior art keywords
plasmid
dna
xylanase
pcx311
escherichia coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
JP58232508A
Other languages
English (en)
Japanese (ja)
Other versions
JPS60126085A (ja
Inventor
Koki Horikoshi
Toshiaki Kudo
Hiroshi Pponda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RIKEN
Original Assignee
RIKEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RIKEN filed Critical RIKEN
Priority to JP58232508A priority Critical patent/JPS60126085A/ja
Priority to DK138984A priority patent/DK138984A/da
Priority to FI840843A priority patent/FI85721C/fi
Priority to EP88121510A priority patent/EP0316023B1/fr
Priority to AT84102440T priority patent/ATE61410T1/de
Priority to EP84102440A priority patent/EP0121138B1/fr
Priority to AT88121510T priority patent/ATE90387T1/de
Priority to DE88121510T priority patent/DE3486163T2/de
Priority to DE8484102440T priority patent/DE3484207D1/de
Priority to CA000449095A priority patent/CA1226833A/fr
Priority to US06/590,636 priority patent/US4624922A/en
Publication of JPS60126085A publication Critical patent/JPS60126085A/ja
Priority to FI890246A priority patent/FI86438C/fi
Publication of JPH0142675B2 publication Critical patent/JPH0142675B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP58232508A 1983-03-08 1983-12-09 新規プラスミド及びその調製方法並びにそのプラスミドを含有する新規微生物 Granted JPS60126085A (ja)

Priority Applications (12)

Application Number Priority Date Filing Date Title
JP58232508A JPS60126085A (ja) 1983-12-09 1983-12-09 新規プラスミド及びその調製方法並びにそのプラスミドを含有する新規微生物
DK138984A DK138984A (da) 1983-03-08 1984-02-29 Plasmid, fremgangsmaade til fremstilling heraf, mikroorganismer indeholdende samme samt fremgangsmaade til dyrkning af mikroorganismen
FI840843A FI85721C (fi) 1983-03-08 1984-03-02 Foerfarande foer extracellulaer produktion av penicillinas, plasmid anvaend vid foerfarandet och foerfarande foer konstruktion av plasmiden.
DE88121510T DE3486163T2 (de) 1983-03-08 1984-03-07 Plasmide, Verfahren zu ihrer Herstellung, sie enthaltende Mikroorganismen und Methoden zur extrazellulären Herstellung von Xylanase durch Züchten dieser Mikroorganismen.
AT84102440T ATE61410T1 (de) 1983-03-08 1984-03-07 Plasmide, verfahren zu ihrer bereitung, mikroorganismen, die diese plasmide enthalten, und verfahren zur kultivierung der mikroorganismen.
EP84102440A EP0121138B1 (fr) 1983-03-08 1984-03-07 Plasmides, méthodes pour la construction de ces plasmides, micro-organismes portant ces plasmides et méthodes pour la cultivation desdits micro-organismes
AT88121510T ATE90387T1 (de) 1983-03-08 1984-03-07 Plasmide, verfahren zu ihrer herstellung, sie enthaltende mikroorganismen und methoden zur extrazellulaeren herstellung von xylanase durch zuechten dieser mikroorganismen.
EP88121510A EP0316023B1 (fr) 1983-03-08 1984-03-07 Plasmides, méthodes pour les construire, micro-organismes les contenant et méthodes de production extracellulaire de xylanase par culture de ces micro-organismes
DE8484102440T DE3484207D1 (de) 1983-03-08 1984-03-07 Plasmide, verfahren zu ihrer bereitung, mikroorganismen, die diese plasmide enthalten, und verfahren zur kultivierung der mikroorganismen.
CA000449095A CA1226833A (fr) 1983-03-08 1984-03-08 Plasmides, methode de construction, microorganismes porteurs desdits plasmides et methode de culture des microorganismes
US06/590,636 US4624922A (en) 1983-12-09 1984-03-19 Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism
FI890246A FI86438C (fi) 1983-03-08 1989-01-17 Plasmider som inducerar extracellulaer sekretion av xylanas, metoder foer framstaellning av dem och metoder foer produktion av xylanas.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP58232508A JPS60126085A (ja) 1983-12-09 1983-12-09 新規プラスミド及びその調製方法並びにそのプラスミドを含有する新規微生物

Publications (2)

Publication Number Publication Date
JPS60126085A JPS60126085A (ja) 1985-07-05
JPH0142675B2 true JPH0142675B2 (fr) 1989-09-13

Family

ID=16940426

Family Applications (1)

Application Number Title Priority Date Filing Date
JP58232508A Granted JPS60126085A (ja) 1983-03-08 1983-12-09 新規プラスミド及びその調製方法並びにそのプラスミドを含有する新規微生物

Country Status (1)

Country Link
JP (1) JPS60126085A (fr)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MOI.GEN.GENET=1983 *

Also Published As

Publication number Publication date
JPS60126085A (ja) 1985-07-05

Similar Documents

Publication Publication Date Title
BG60920B2 (bg) Метод за стабилна ген амплификация на хромозомна днк на прокариотични микроорганизми
US4624922A (en) Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism
JPH0142675B2 (fr)
JP2620852B2 (ja) 新規プラスミド及びそれにより形質転換された新規微生物
Lejeune et al. Cloning of an endoglucanase gene from Pseudomonas fluorescens var. cellulosa into Escherichia coli and Pseudomonas fluorescens
EP0121138A1 (fr) Plasmides, méthodes pour la construction de ces plasmides, micro-organismes portant ces plasmides et méthodes pour la cultivation desdits micro-organismes
JPH0375153B2 (fr)
JPH0142674B2 (fr)
JPH0355105B2 (fr)
DE69429479T2 (de) Dna fragment welches ein gen für eine alkaline pullulanase beinhaltet
JP2833793B2 (ja) ヘパリナーゼをコードするプラスミド、このプラスミドを保持するヘパリナーゼ生産株及びヘパリナーゼの製造法
JPH0817702B2 (ja) 新規プラスミド及びその調製方法並びにそのプラスミドを含有する新規微生物
US4962055A (en) Plasmid, method for construction of the same, microorganisms carrying the plasmid and method for cultivation of the microorganism
EP0316023B1 (fr) Plasmides, méthodes pour les construire, micro-organismes les contenant et méthodes de production extracellulaire de xylanase par culture de ces micro-organismes
JP3399685B2 (ja) コレステロールオキシダーゼ活性を有する改変酵素
US5246839A (en) Secretion plasmid comprising the kilgene
JP2913411B2 (ja) セルラーゼ遺伝子
FI86438B (fi) Plasmider som inducerar extracellulaer sekretion av xylanas, metoder foer framstaellning av dem och metoder foer produktion av xylanas.
JP2592289B2 (ja) アルカリセルラーゼ遺伝子を含有するｄｎａ断片並びに該ｄｎａ断片を組み込んだベクター及びその含有微生物
JP2814177B2 (ja) アルカリプルラナーゼ遺伝子を含有するｄｎａ断片、該ｄｎａ断片を組み込んだベクター及び該ベクターを有する微生物
JPH06102022B2 (ja) 組換え体ｄｎａ、その製造法、およびそれを含むバチルス属細菌
JP2681634B2 (ja) 耐熱性液化型アミラーゼ生産能力の増強された細菌新菌株
JP2928265B2 (ja) Ｄｎａ断片、微酸性微低温性セルラーゼ及びその製造方法
JPS62275684A (ja) 組換えベクタ−及びそれを有する細菌
JPS62277A (ja) 新規プラスミド及びそれにより形質転換された新規微生物及びそれを用いた有用生理活性物質の製造法