JPH02135089A - ラッカーゼ及びその製造方法 - Google Patents

ラッカーゼ及びその製造方法

Info

Publication number: JPH02135089A
Authority: JP; Japan
Prior art keywords: laccase; enzyme; coccineus; culture; activity
Prior art date: 1988-11-16
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

JP28974588A

Other languages

English (en)

Japanese (ja)

Other versions

JPH0544271B2 (fr

Inventor

Kadoaki Oda

小田　圭昭

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

NIKKA KASEI KK

Original Assignee

NIKKA KASEI KK

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-11-16

Filing date

1988-11-16

Publication date

1990-05-23

1988-11-16 Application filed by NIKKA KASEI KK filed Critical NIKKA KASEI KK

1988-11-16 Priority to JP28974588A priority Critical patent/JPH02135089A/ja

1990-05-23 Publication of JPH02135089A publication Critical patent/JPH02135089A/ja

1993-07-05 Publication of JPH0544271B2 publication Critical patent/JPH0544271B2/ja

Status Granted legal-status Critical Current

Links

108010029541 Laccase Proteins 0.000 title claims abstract description 44
238000004519 manufacturing process Methods 0.000 title claims description 9
108090000790 Enzymes Proteins 0.000 claims abstract description 64
102000004190 Enzymes Human genes 0.000 claims abstract description 64
230000000694 effects Effects 0.000 claims abstract description 27
230000001590 oxidative effect Effects 0.000 claims abstract description 11
RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 10
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 10
239000001301 oxygen Substances 0.000 claims abstract description 10
229910052760 oxygen Inorganic materials 0.000 claims abstract description 10
QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 claims abstract description 9
229910052802 copper Inorganic materials 0.000 claims abstract description 9
239000010949 copper Substances 0.000 claims abstract description 9
RLSSMJSEOOYNOY-UHFFFAOYSA-N m-cresol Chemical compound CC1=CC=CC(O)=C1 RLSSMJSEOOYNOY-UHFFFAOYSA-N 0.000 claims abstract description 8
239000000758 substrate Substances 0.000 claims abstract description 7
XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims abstract description 6
244000005700 microbiome Species 0.000 claims abstract description 6
RZXMPPFPUUCRFN-UHFFFAOYSA-N p-toluidine Chemical compound CC1=CC=C(N)C=C1 RZXMPPFPUUCRFN-UHFFFAOYSA-N 0.000 claims abstract description 6
VOWZNBNDMFLQGM-UHFFFAOYSA-N 2,5-dimethylaniline Chemical group CC1=CC=C(C)C(N)=C1 VOWZNBNDMFLQGM-UHFFFAOYSA-N 0.000 claims abstract description 5
241000041999 Trametes coccinea Species 0.000 claims abstract description 4
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims abstract description 4
150000002989 phenols Chemical class 0.000 claims abstract description 4
GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 claims abstract description 3
239000004201 L-cysteine Substances 0.000 claims abstract description 3
235000013878 L-cysteine Nutrition 0.000 claims abstract description 3
AFBPFSWMIHJQDM-UHFFFAOYSA-N N-methyl-N-phenylamine Natural products CNC1=CC=CC=C1 AFBPFSWMIHJQDM-UHFFFAOYSA-N 0.000 claims abstract description 3
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-1 methoxyl group Chemical group 0.000 claims description 3
239000004922 lacquer Substances 0.000 abstract description 15
125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 abstract description 2
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YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 18
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238000000108 ultra-filtration Methods 0.000 description 4
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
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XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 3
238000000746 purification Methods 0.000 description 3
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CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 description 2
DXRKLUVKXMAMOV-UHFFFAOYSA-N 3-heptadecylcatechol Chemical compound CCCCCCCCCCCCCCCCCC1=CC=CC(O)=C1O DXRKLUVKXMAMOV-UHFFFAOYSA-N 0.000 description 2
241000221198 Basidiomycota Species 0.000 description 2
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COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
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239000012507 Sephadex™ Substances 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 2
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
229910052921 ammonium sulfate Inorganic materials 0.000 description 2
235000011130 ammonium sulphate Nutrition 0.000 description 2
238000004364 calculation method Methods 0.000 description 2
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239000003153 chemical reaction reagent Substances 0.000 description 2
238000002983 circular dichroism Methods 0.000 description 2
238000012258 culturing Methods 0.000 description 2
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239000001963 growth medium Substances 0.000 description 2
229960001867 guaiacol Drugs 0.000 description 2
239000000411 inducer Substances 0.000 description 2
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PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 description 1
QARRXYBJLBIVAK-UEMSJJPVSA-N 3-[(8e,11e)-pentadeca-8,11-dienyl]benzene-1,2-diol;3-[(8e,11e)-pentadeca-8,11,14-trienyl]benzene-1,2-diol;3-[(8e,11e,13e)-pentadeca-8,11,13-trienyl]benzene-1,2-diol;3-[(e)-pentadec-8-enyl]benzene-1,2-diol;3-pentadecylbenzene-1,2-diol Chemical compound CCCCCCCCCCCCCCCC1=CC=CC(O)=C1O.CCCCCC\C=C\CCCCCCCC1=CC=CC(O)=C1O.CCC\C=C\C\C=C\CCCCCCCC1=CC=CC(O)=C1O.C\C=C\C=C\C\C=C\CCCCCCCC1=CC=CC(O)=C1O.OC1=CC=CC(CCCCCCC\C=C\C\C=C\CC=C)=C1O QARRXYBJLBIVAK-UEMSJJPVSA-N 0.000 description 1
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QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
DQTMTQZSOJMZSF-UHFFFAOYSA-N urushiol Natural products CCCCCCCCCCCCCCCC1=CC=CC(O)=C1O DQTMTQZSOJMZSF-UHFFFAOYSA-N 0.000 description 1
238000005406 washing Methods 0.000 description 1
239000002023 wood Substances 0.000 description 1
239000011701 zinc Substances 0.000 description 1
229910052725 zinc Inorganic materials 0.000 description 1

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Enzymes And Modification Thereof (AREA)

JP28974588A 1988-11-16 1988-11-16 ラッカーゼ及びその製造方法 Granted JPH02135089A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP28974588A JPH02135089A (ja)	1988-11-16	1988-11-16	ラッカーゼ及びその製造方法

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Application Number	Priority Date	Filing Date	Title
JP28974588A JPH02135089A (ja)	1988-11-16	1988-11-16	ラッカーゼ及びその製造方法

Publications (2)

Publication Number	Publication Date
JPH02135089A true JPH02135089A (ja)	1990-05-23
JPH0544271B2 JPH0544271B2 (fr)	1993-07-05

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ID=17747211

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP28974588A Granted JPH02135089A (ja)	1988-11-16	1988-11-16	ラッカーゼ及びその製造方法

Country Status (1)

Country	Link
JP (1)	JPH02135089A (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS6394972A (ja) *	1986-10-13	1988-04-26	Bio-Le Kk	ラツカ−ゼの製造法

1988
- 1988-11-16 JP JP28974588A patent/JPH02135089A/ja active Granted

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS6394972A (ja) *	1986-10-13	1988-04-26	Bio-Le Kk	ラツカ−ゼの製造法

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Publication number	Publication date
JPH0544271B2 (fr)	1993-07-05

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