JPH025759Y2 - - Google Patents

Description

[Detailed Description of the Invention] [Field of Application of the Invention] The present invention relates to a fermentation liquid sample collection device for a fermenter, and particularly to an aseptic fermentation liquid sample collection device suitable for a fermentation tank in a biohazard facility.

[Background of the idea]

The conventional fermentation liquid sample collection device for fermenters is the "Biochemical Engineering" Foundation, University of Tokyo Press, 1976.
Published on November 25th, page 322, this is explained using Figure 2.The fermentation liquid sample collection first valve 1 is provided with a steam inflow valve 2 and a sample collection second valve 3. Tank 4
Although it is possible to sample the fermentation liquid more aseptically, there is a drawback that the microorganisms in the fermentation liquid contaminate the surrounding air because the fermentation liquid sample comes into direct contact with the outside air. In particular, where the microorganisms are dangerous to the surrounding biological system, conventionally designed sample collection devices cannot be used.

[Purpose of invention]

The purpose of this invention is to treat the fermentation liquid aseptically, and
To provide a fermentation liquid sample collection device for collecting a fermentation liquid sample while preventing microorganisms in the fermentation liquid from contaminating the surrounding area.

[Summary of the idea]

In this invention, a fermentation liquid sampling line equipped with a steam line for heat sterilization, a sample collection pot unit, and a drain line are removably connected via a coupler to aseptically and ferment the fermentation liquid. Fermentation liquid samples are collected so that the liquid samples do not come into direct contact with the outside air.

[Example of idea]

An embodiment of the present invention will be described below with reference to FIG. The sampling line 15 connected to the fermentation liquid sample collection port provided on the side of the fermentation tank 4 includes a first fermentation liquid sample collection valve 1, a steam inflow valve 2, a sterile air inflow valve 5, and a second sample collection valve 3. , and a coupler 6 are provided. Furthermore, the coupler 6 has a T-joint 16.
A valve 7, an upper lid 8 provided with a breathing port 9, and a sample pot 10 are attached through the T.
A flexible hose 1 is connected to the other side of the coupling 16 via a coupler 11 having the same specifications as the coupler 6.
2. The drain valve 13 is connected to the drain line 14. In addition, the upper lid 8 and sample pot 10
It can be attached and detached by screw connection etc.
The sample pot 10 can be easily replaced. Furthermore, a breathing hole 9 provided in the upper lid 8
A cotton plug is provided during use to maintain sterility.

In the above-mentioned configuration, fermentation liquid sample collection will be explained. First, if there is no sample collection operation, connect the coupler 11 directly to the coupler 6 (in this case, the T-joint 16, valve 7, upper lid 8, and sample pot 10 are removed).
By opening the steam inlet valve 2 and the second sampling valve 3, introducing steam into the sampling line 15, and intermittently opening the drain valve 13, a steam seal is performed and the sterility of the system is maintained. Therefore, when performing a sample collection operation, the unit consisting of the T-joint 16, valve 7, upper lid 8, and sample pot 10, which has been sterilized in an autoclave (not shown), is removed by removing the coupler 6 and the coupler 11. Install as shown in Figure 1. At this time, valve 7 is closed. The first valve operation is to open the steam inlet valve 2, the second sample collection valve 3, and the drain valve 13 to introduce steam into the sampling line 15 in order to sterilize the coupler 6, coupler 11, and T-joint 16. When sterilization is completed, the steam inflow valve 2 is closed, the sterile air inflow valve 5 is opened, and the sampling line 15 is cooled. When cooling is completed, the second sample collection valve 3 and drain valve 13 are closed, the first fermentation liquid sample collection valve 1 is opened, liquid is pushed, and the sterile air inflow valve 5 is closed to complete the pre-operation. After that, in order to collect the fermentation liquor sample, the sample collection stage 2 is used.
Valve 3 and valve 7 are opened to introduce the fermentation liquid sample into sample pot 10. Sample collection is completed with the above operations. After collecting the sample,
After the sampling line 15 is sterilized, the unit consisting of the coupler 6, coupler 11, T-joint 16, valve 7, top lid 8, and sample pot 10 containing the sample is removed and brought into a biohazard safety cabinet for necessary analysis. can be done.

[Effect of idea]

As described above, according to the present invention, it is possible to maintain the fermentation liquid in a completely sterile manner during the sample collection process, and to prevent the fermentation liquid from scattering to the surroundings or coming into contact with the outside air during sample collection. Fermentation liquid samples can be collected aseptically from fermenters in biohazard facilities.

[Brief explanation of the drawing]

FIG. 1 is a system diagram showing an embodiment of the aseptic fermentation liquid sample collection device for a fermenter according to the present invention, and FIG. 2 is a system diagram of a conventional fermentation liquid sample collection device. 1... Fermentation liquid sample collection first valve, 2...
Steam inflow valve, 3... Second sample collection valve, 4... Fermenter, 5... Sterile air inflow valve, 6, 11... Coupler, 7... Valve, 8
... Top lid, 9 ... Breathing port, 10 ... Sample pot, 12 ... Flexible hose, 13 ... Drain valve, 14 ... Drain line, 15 ... Sampling line, 16 ... T-joint.

Claims (1)

[Scope of Claim for Utility Model Registration] A first fermentation liquid sample collection valve is provided on the sampling port side of a sampling line connected to the fermentation liquid sample collection port of the fermentation tank, and a second sample collection valve is provided on the end side of the sampling line. , a fermentation liquid sample collection device that collects a fermentation liquid sample aseptically by providing a steam line for heat sterilization between the first fermentation liquid sample collection valve and the second sample collection valve, wherein the fermentation liquid sample is collected in the sampling line. A sterile air line is connected between the first valve and the second sample collection valve, and the second sample collection valve provided at the end of the sampling line is removably connected to the sample collection pot unit and the drain line via a coupler. An aseptic fermentation liquid sample collection device for a fermentation tank, characterized in that: