JPH0272860A - Laser irradiating device for live cell - Google Patents
Laser irradiating device for live cellInfo
- Publication number
- JPH0272860A JPH0272860A JP22464488A JP22464488A JPH0272860A JP H0272860 A JPH0272860 A JP H0272860A JP 22464488 A JP22464488 A JP 22464488A JP 22464488 A JP22464488 A JP 22464488A JP H0272860 A JPH0272860 A JP H0272860A
- Authority
- JP
- Japan
- Prior art keywords
- live cell
- irradiating device
- laser irradiating
- cells
- living cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】 〔産業上の利用分野１ 本発明は生細胞レーザ照射装置に関する。[Detailed description of the invention] [Industrial application field 1 The present invention relates to a live cell laser irradiation device.
［従来の技術１
生細胞への遺伝子等の注入方法の中で、すぐれた機能を
有する方法として９例えば、特公昭６２−７８３７号公
報に記載のようなレーザ光による穿孔技術がある。[Prior Art 1] Among the methods of injecting genes, etc. into living cells, there is a method having excellent functionality9, for example, a perforation technique using a laser beam as described in Japanese Patent Publication No. 7837/1983.
この技術は遺伝子等を含む液中に生細胞を浸しておき、
生細胞にレーザ光をレンズを通して集光させることによ
り細胞膜の透過性を一時的に向上させ、遺伝子等を生細
胞内に取り込ませるものである。This technique involves immersing living cells in a solution containing genes, etc.
By focusing laser light on living cells through a lens, the permeability of cell membranes is temporarily improved and genes, etc. are incorporated into living cells.
［発明が解決しようとする課題］
上記従来技術において、生細胞を収納する容器としては
第３図に示すような穴あけ加工を施した培養ビン５が使
用されていた。この穴あけ加工をした部分にカバーグラ
ス６を貼り付け、その内側に生細胞２を付着させる。こ
の方法では上方よりレーザ光４を照射する場合、付着性
の細胞にしか適用できず、浮遊性の細胞には適用するこ
とができなかった。[Problems to be Solved by the Invention] In the above-mentioned prior art, a culture bottle 5 with holes as shown in FIG. 3 was used as a container for storing living cells. A cover glass 6 is attached to the hole-drilled area, and living cells 2 are attached to the inside of the cover glass 6. In this method, when the laser beam 4 is irradiated from above, it can only be applied to adherent cells and cannot be applied to floating cells.
本発明の目的は、浮遊性の細胞にも適用可能な生細胞収
納容器を提供することにある。An object of the present invention is to provide a living cell storage container that can also be applied to floating cells.
［課題を解決するための手段］
上記目的は、浮遊性細胞と生細胞収納容器の上面との距
離がレーザ光照射用レンズの焦点距離以下になるように
することにより達成される。[Means for Solving the Problems] The above object is achieved by making the distance between the floating cells and the upper surface of the living cell container equal to or less than the focal length of the laser beam irradiation lens.
〔作 用］
このための生細胞収納容器としては、生細胞をレーザ照
射用レンズの焦点距離より小さな間隔ではさみこむ構造
とすればよい。[Function] The living cell storage container for this purpose may have a structure in which living cells are sandwiched at an interval smaller than the focal length of the laser irradiation lens.
以下１本発明の一実施例を第１図により説明す杭
第１図に示すようにレーザ光の波長に対する透過率の優
れた透明筒ｌの中に生細胞２を含んだ溶液を入れ、その
両端を栓３で密閉する。この方法により浮遊性細胞に対
しても、レーザ光４を上方より照射することができる。Below, one embodiment of the present invention will be explained with reference to Fig. 1. As shown in Fig. 1, a solution containing living cells 2 is placed in a transparent cylinder l with excellent transmittance to the wavelength of laser light. Seal both ends with stoppers 3. By this method, even floating cells can be irradiated with laser light 4 from above.
透明筒１が円筒形の場合、レーザ光４の屈折により焦光
部の形状が変形するため、第２図のような矩形断面をし
たものを使用した方が望ましい。If the transparent tube 1 is cylindrical, the shape of the focal part will be deformed due to the refraction of the laser beam 4, so it is preferable to use one with a rectangular cross section as shown in FIG.
更に、透明筒１の両端の栓３を設ける代りに透明筒５の
端部を加熱融着して密閉するようにしてもよい。Furthermore, instead of providing the plugs 3 at both ends of the transparent tube 1, the ends of the transparent tube 5 may be heat-fused and sealed.
［発明の効果］
本発明によれば生細胞、特に浮遊性細胞へのレーザ光照
射が容易になり、実験効率の向上や安全性の向上に効果
がある。[Effects of the Invention] According to the present invention, it becomes easy to irradiate living cells, especially floating cells, with laser light, and it is effective in improving experimental efficiency and safety.
第１図は９本発明の一実施例を示す生細胞収納容器の縦
断面図、第２図は９本発明の他の実施例を示す生細胞収
納容器の第１図とは直交方向の断面図、第３図は、従来
の生細胞収納容器の縦断面図である。
ｉ −−−−−一透明筒、 ２−−−−−一生細胞。FIG. 1 is a vertical cross-sectional view of a living cell storage container showing one embodiment of the present invention, and FIG. 2 is a cross-sectional view in a direction perpendicular to FIG. FIG. 3 is a vertical sectional view of a conventional living cell storage container. i ---- One transparent cylinder, 2 ---- Life cell.
Claims (1)
を収納する容器として両端を閉塞した透明な筒を用いる
ことを特徴とする生細胞レーザ照射装置1. A laser beam irradiation device for living cells, characterized in that a transparent tube with both ends closed is used as a container for storing the living cells.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22464488A JPH0272860A (en) | 1988-09-09 | 1988-09-09 | Laser irradiating device for live cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22464488A JPH0272860A (en) | 1988-09-09 | 1988-09-09 | Laser irradiating device for live cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0272860A true JPH0272860A (en) | 1990-03-13 |
Family
ID=16816948
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP22464488A Pending JPH0272860A (en) | 1988-09-09 | 1988-09-09 | Laser irradiating device for live cell |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0272860A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3401665A1 (en) * | 2017-05-12 | 2018-11-14 | University College Dublin National University Of Ireland, Dublin | A system and device for analysis of specific matter in liquid samples by optical microscopy |
-
1988
- 1988-09-09 JP JP22464488A patent/JPH0272860A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3401665A1 (en) * | 2017-05-12 | 2018-11-14 | University College Dublin National University Of Ireland, Dublin | A system and device for analysis of specific matter in liquid samples by optical microscopy |
| WO2018206802A1 (en) | 2017-05-12 | 2018-11-15 | University College Dublin, National University Of Ireland, Dublin | A system and device |
| JP2020519899A (en) * | 2017-05-12 | 2020-07-02 | ユニバーシティ・カレッジ・ダブリン，ナショナル・ユニバーシティ・オブ・アイルランド，ダブリン | Systems and devices |
| AU2018265597B2 (en) * | 2017-05-12 | 2024-01-04 | University College Dublin, National University Of Ireland, Dublin | A system and device |
| US12370542B2 (en) | 2017-05-12 | 2025-07-29 | University College Dublin, National University Of Ireland, Dublin | System and device |
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