JPH0436634Y2 - - Google Patents

Description

[Detailed Description of the Invention] (Industrial Application Field) The present invention relates to a cell culture cell used in a method of transferring DNA, etc. into living cells by irradiating living cells with a laser beam under a microscope.

(Prior Art) The present inventors previously filed a patent application for a device for transferring DNA, etc. into living cells by irradiating them with a laser beam (Japanese Patent Application No. 191378/1982).

In this case, the living cells in the culture cell are irradiated with a laser beam, but the living cells must be placed within the focal plane of the laser beam, and in order to irradiate the laser while the cells are alive, they must be immersed in the culture medium. It is necessary that the However, since the culture solution has low transmittance to ultraviolet light, the cells are attached to a cover glass such as a quartz plate and a laser is irradiated through the plate. The culture cell used in this case has the configuration shown in FIG. 5, which is a longitudinal cross-sectional view, and FIG. 6, which is a cross-sectional view thereof. As shown in the figure, this cell culture cell 1 is provided with an opening 3 in addition to a culture solution inlet 2 for injecting a culture solution into the cell, and living cells 4 are planted in advance in this opening 3. A cover glass 5 is provided via a rubber packing 6. In use, the culture cell 1 is raised, the culture solution 7 is filled into the cell 1 through the culture solution inlet 2, and the culture solution inlet 2 is plugged with a stopper 8. This is placed under a microscope 9 as shown in the figure, and a laser beam 10 focused on the culture cell 1 by the microscope 9 is irradiated onto the cover glass 5, that is, the living cells.

(Problem that the invention aims to solve) However, in such a cell culture cell, when the culture solution inlet is plugged after filling with culture solution, air bubbles always remain, and if the cell is left to rest in this state, the opening, that is, the cover, will remain. These air bubbles accumulate in the lower part of the glass. In this case, some of the living cells attached to the cover glass dry and die. The present invention relates to the improvement of this cell culture cell,
The purpose is to keep living cells alive.

(Means for solving the problem) The above problem can be solved by using a cell culture cell in which a bubble reservoir is provided between the opening where the cover glass with living cells attached is installed and the culture solution inlet. resolved.

(Operations and Effects) When the cell culture cell of the present invention is used, air bubbles made of residual air in the culture medium enter the air bubble reservoir, preventing air bubbles from entering the opening, and keeping living cells in a completely alive state. It can be irradiated. Note that the present invention is also effective when used only for microscopic observation of living cells.

(Example) Hereinafter, the cell culture cell of the present invention will be explained in detail using the drawings. FIG. 1 is a longitudinal cross-sectional view of one embodiment of the cell culture cell of the present invention, and FIG.
FIG.

A cell culture cell 1 according to the present invention has an opening 3 protruding outward between an opening 3 in which a cover glass 5 with living cells 4 attached is installed and a culture solution inlet 2 for injecting a culture solution into the cell 1. It has a bubble reservoir 11.
Therefore, after injecting a culture solution into the culture cell 1 and then plugging the culture solution inlet with a stopper 8, the air bubbles present in the culture cell 1 are placed in the bubble reservoir 11, and other parts, especially the cover glass 5, are placed. Air bubbles located on the back side can be avoided.

FIG. 3 is a longitudinal cross-sectional view of another embodiment of the cell culture cell of the present invention, and FIG. 4 is a cross-sectional view thereof.

In this embodiment, the bubble reservoir 11 is continuous from the vicinity of the culture solution inlet 2 of the cell culture cell 1, and the upper wall of the bubble reservoir 11 becomes higher as the distance from the culture solution inlet 2 increases. Since the culture cell 1 is configured so that the culture cell 1 can be
The air bubbles inside naturally enter the air bubble reservoir 11.

[Brief explanation of the drawing]

FIG. 1 is a longitudinal sectional view of one embodiment of the cell culture cell of the present invention, FIG. 2 is a cross-sectional view thereof, and FIG. 3 is a longitudinal sectional view of another embodiment of the cell culture cell of the present invention. , FIG. 4 is a cross-sectional view thereof, FIG. 5 is a vertical cross-sectional view of a conventional cell culture cell, and FIG. 6 is a cross-sectional view of FIG. 5. 1...Cell culture cell, 2...Culture solution inlet, 3
...Opening, 4...Living cells, 5...Cover glass, 6...Rubber packing, 7...Culture solution, 8...
...Plug, 9...Microscope, 10...Laser beam,
11...Bubble reservoir.

Claims (1)

[Scope of utility model registration request] In a cell culture cell having an opening in which a cover glass with living cells attached is installed, an air bubble reservoir is provided between a culture solution inlet for injecting a culture solution into the cell culture cell and the opening. A cell culture cell characterized by: